FvH4_6g22000

Thioredoxin-like 3-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011087
Physical Location & Seq
Reverse (-)
15678910 .. 15679946
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g22000.t1

Sequence Viewer

Length: 558 bp
ATGTCTGTTCTGGCAGCAAATTCTCACATTTTCTGTAGAGAAGTTCATCAAAGAGAGCATCAACAACAGTTTTGCAGTGCTGCAGGGGGCAGTTTTGCTTTGCCAAAATCTTCTGGGTTTGGTTTTTTCTATAGAAACAGAGATAGCAAGAAGATATTGAGAAGAGACTTGAAAGCGGAGGCCTTTTGGCCAGAGTCAAGACCCACTTTTGTAGAGATGGAATCAATAAACGACTCTGATCACCTTGATCATATATTAAGCCATGCTCAACAGCTCTCCCAGCCCATTGTCATTGATTGGATGGCAGCTTGGTGCCGCAAATGCATCTATCTGAAGCCTAAGTTGGAGAAATTGGCTGCTGAATATGAAACCAAGGTCAAATTCTATTATGTTGATGTCAACAAGGTGCCTCAAACTCTAGTGAAACGCGGGAACATTTCTAAAATGCCAACCATTCAGGTATGGAAAGATGGGGAGTTTAAAGAAGAAGTGATTGGAGGTCACAAAGCATGGCTTGTGATTGATGAAGTGCGAGAAATGATCCAAAAATTTGTGTGA

Protein Analysis

186

Amino Acids

21.55

Weight (kDa)

8.83

Isoelectric Point (pI)

52.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 90 - 169 1.3e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06690 AT5G06690 AT5G06690 AT5G06690
fragaria_vesca FvH4_6g22000
malus_domestica MD12G1055400.v1.1 MD14G1054800.v1.1
prunus_persica Prupe.7G079900_v2.0.a1
pyrus_communis pycom12g04840
rosa_chinensis RchiOBHm_Chr3g0479301
rosa_laevigata RLG00000023585
rosa_multiflora Rmu_sc0026945.1_g000002
rosa_roxburghii Rroxscaffold_6G00402420
rosa_rugosa Rorug03G0174200 Rorug03G0174200
rosa_samantha Rh3AG225300 Rh3BG257800 Rh3CG253500 Rh3DG249700
rosa_wichuraiana Rw3G020150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 312, 406
AccII CGCG 1 cut(s) 429
AciI CCGC 3 cut(s) 176, 316, 429
AclWI GGATC 1 cut(s) 535
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 3 cut(s) 19, 380, 548
AcuI CTGAAG 1 cut(s) 353
AgsI TTSAA 1 cut(s) 172
AjuI GAANNNNNNNTTGG 6 cut(s) 326, 358, 365, 397, 477, 509
AluBI AGCT 2 cut(s) 274, 308
AluI AGCT 2 cut(s) 274, 308
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 1 cut(s) 535
AoxI GGCC 2 cut(s) 180, 188
ApeKI GCWGC 4 cut(s) 14, 80, 305, 356
ApoI RAATTY 3 cut(s) 19, 380, 548
AsuHPI GGTGA 1 cut(s) 233
BalI TGGCCA 1 cut(s) 190
BanI GGYRCC 2 cut(s) 312, 406
BbvI GCAGC 4 cut(s) 26, 67, 317, 343
BccI CCATC 3 cut(s) 211, 295, 464
BclI TGATCA 2 cut(s) 238, 247
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 3 cut(s) 34, 81, 130
BisI GCNGC 5 cut(s) 15, 81, 306, 316, 357
BlsI GCNGC 5 cut(s) 16, 82, 307, 317, 358
BmiI GGNNCC 2 cut(s) 314, 408
BmsI GCATC 2 cut(s) 67, 333
BsaJI CCNNGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 306
BseXI GCAGC 4 cut(s) 26, 67, 317, 343
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 2 cut(s) 182, 190
BshNI GGYRCC 2 cut(s) 312, 406
BsmAI GTCTC 1 cut(s) 159
BsnI GGCC 2 cut(s) 182, 190
Bsp143I GATC 3 cut(s) 238, 247, 540
BspACI CCGC 3 cut(s) 176, 316, 429
BspANI GGCC 2 cut(s) 182, 190
BspFNI CGCG 1 cut(s) 429
BspLI GGNNCC 2 cut(s) 314, 408
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 1 cut(s) 535
BspT107I GGYRCC 2 cut(s) 312, 406
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 238, 247, 540
BssT1I CCWWGG 1 cut(s) 372
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 157
BstDEI CTNAG 1 cut(s) 339
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 429
BstKTI GATC 3 cut(s) 241, 250, 543
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 3 cut(s) 238, 247, 540
BstMWI GCNNNNNNNGC 2 cut(s) 280, 321
BstSFI CTRYAG 3 cut(s) 34, 81, 130
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 4 cut(s) 26, 67, 317, 343
BsuRI GGCC 2 cut(s) 182, 190
BtsCI GGATG 1 cut(s) 306
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
CviAII CATG 2 cut(s) 263, 510
CviJI RGCY 9 cut(s) 182, 190, 261, 274, 283, 308, 337, 356, 514
CviKI_1 RGCY 9 cut(s) 182, 190, 261, 274, 283, 308, 337, 356, 514
DdeI CTNAG 1 cut(s) 339
DpnI GATC 3 cut(s) 240, 249, 542
DpnII GATC 3 cut(s) 238, 247, 540
DraI TTTAAA 1 cut(s) 481
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 353
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 2 cut(s) 266, 513
FaiI YATR 8 cut(s) 132, 252, 254, 264, 366, 390, 463, 511
FalI AAGNNNNNCTT 4 cut(s) 190, 222, 498, 530
FatI CATG 2 cut(s) 262, 509
FauI CCCGC 1 cut(s) 422
FbaI TGATCA 2 cut(s) 238, 247
Fnu4HI GCNGC 5 cut(s) 15, 81, 306, 316, 357
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 15, 81, 306, 316, 357
FspBI CTAG 1 cut(s) 419
GluI GCNGC 5 cut(s) 15, 81, 306, 316, 357
GsaI CCCAGC 1 cut(s) 283
HaeIII GGCC 2 cut(s) 182, 190
Hin1II CATG 2 cut(s) 266, 513
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 3 cut(s) 194, 221, 233
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 2 cut(s) 238, 333
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 1 cut(s) 400
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 3 cut(s) 75, 83, 324
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 321
HpyF3I CTNAG 1 cut(s) 339
Hsp92II CATG 2 cut(s) 266, 513
Ksp22I TGATCA 2 cut(s) 238, 247
Kzo9I GATC 3 cut(s) 238, 247, 540
LpnPI CCDG 5 cut(s) 69, 99, 204, 293, 443
Lsp1109I GCAGC 4 cut(s) 26, 67, 317, 343
LweI GCATC 2 cut(s) 67, 333
MaeI CTAG 1 cut(s) 419
MaeIII GTNAC 1 cut(s) 500
MalI GATC 3 cut(s) 240, 249, 542
MboI GATC 3 cut(s) 238, 247, 540
MboII GAAGA 4 cut(s) 102, 163, 174, 497
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 4 cut(s) 19, 350, 380, 548
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 2 cut(s) 203, 227
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 3 cut(s) 172, 420, 491
Mox20I TGGCCA 1 cut(s) 190
Mph1103I ATGCAT 1 cut(s) 326
MscI TGGCCA 1 cut(s) 190
MseI TTAA 2 cut(s) 257, 480
Msp20I TGGCCA 1 cut(s) 190
MvnI CGCG 1 cut(s) 429
MwoI GCNNNNNNNGC 2 cut(s) 280, 321
NdeII GATC 3 cut(s) 238, 247, 540
NlaIII CATG 2 cut(s) 266, 513
NlaIV GGNNCC 2 cut(s) 314, 408
NmuCI GTSAC 1 cut(s) 500
NsiI ATGCAT 1 cut(s) 326
PceI AGGCCT 1 cut(s) 182
PfeI GAWTC 1 cut(s) 221
PkrI GCNGC 5 cut(s) 16, 82, 307, 317, 358
PleI GAGTC 2 cut(s) 202, 227
PpsI GAGTC 2 cut(s) 202, 227
PspFI CCCAGC 1 cut(s) 279
PspN4I GGNNCC 2 cut(s) 314, 408
PstI CTGCAG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 257, 480
SatI GCNGC 5 cut(s) 15, 81, 306, 316, 357
Sau3AI GATC 3 cut(s) 238, 247, 540
SchI GAGTC 2 cut(s) 203, 227
SetI ASST 7 cut(s) 246, 276, 310, 378, 408, 462, 502
SfaNI GCATC 2 cut(s) 67, 333
SfcI CTRYAG 3 cut(s) 34, 81, 130
Sse9I AATT 4 cut(s) 19, 350, 380, 548
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 3 cut(s) 176, 316, 429
SspMI CTAG 1 cut(s) 419
StuI AGGCCT 1 cut(s) 182
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 69
TasI AATT 4 cut(s) 19, 350, 380, 548
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 221
Tru1I TTAA 2 cut(s) 257, 480
Tru9I TTAA 2 cut(s) 257, 480
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 4 cut(s) 14, 80, 305, 356
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 3 cut(s) 35, 381, 540
TspRI CASTG 1 cut(s) 82
XapI RAATTY 3 cut(s) 19, 380, 548
XspI CTAG 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.