MD14G1054800.v1.1

Thioredoxin-like 3-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011087
Physical Location & Seq
Reverse (-)
5541353 .. 5542962
1610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1054800.v1.1.491

Sequence Viewer

Length: 573 bp
ATGTCTGTTTTGGCAGCCAATTCTCACGTTCTGTGTAGCAGACAAGTCCGTCACAGTCACAGAGACCAACAGCCACAGTTCTGGAGCAATGGCGGCGGTTTGCTGTTGTTGCCAAAAGCTTCTGGATTAGGGTTTATGGACAGAAACAGAGATGGCAACAAAACGTTGAAGAGGGACTTGAAAGTTGAGGCCTTTTGGGATACGGATACCTCGAGTCCCACGGTTGTGGAGTTGGAAGCAATCAATGACTGTCATCAGCTTGATCAGATTCTTAGCCATGCACAGGAGAGCTCTCTGCCAGTTGTCATTGATTGGATGGCATCTTGGTGCCGCAAATGCATCTATTTGAAGCCTAAATTGGAGAAATTCGCTGCTGAATATGACACCAAGATCAAATTCTACTATGTTGATGTCAACAAAGTACCTCAAGCTCTAGTAAAGCGTGGAAATATCTCTAAAATGCCTACAATTCAGGTATGGAAAGATGGAGAGTACAAAGCAGAAGTGATTGGGGGACATAAAGCATGGCTTGTGCTTGAAGAAGTGAGAGAAATGCTCAAAAACTTTGTGTGA

Protein Analysis

191

Amino Acids

21.76

Weight (kDa)

8.61

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 95 - 174 3.5e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06690 AT5G06690 AT5G06690 AT5G06690
fragaria_vesca FvH4_6g22000
malus_domestica MD12G1055400.v1.1 MD14G1054800.v1.1
prunus_persica Prupe.7G079900_v2.0.a1
pyrus_communis pycom12g04840
rosa_chinensis RchiOBHm_Chr3g0479301
rosa_laevigata RLG00000023585
rosa_multiflora Rmu_sc0026945.1_g000002
rosa_roxburghii Rroxscaffold_6G00402420
rosa_rugosa Rorug03G0174200 Rorug03G0174200
rosa_samantha Rh3AG225300 Rh3BG257800 Rh3CG253500 Rh3DG249700
rosa_wichuraiana Rw3G020150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 327
AciI CCGC 3 cut(s) 93, 96, 331
AclI AACGTT 1 cut(s) 164
AcsI RAATTY 2 cut(s) 365, 395
AfaI GTAC 2 cut(s) 423, 494
AfiI CCNNNNNNNGG 1 cut(s) 283
AgsI TTSAA 4 cut(s) 169, 181, 349, 539
AjuI GAANNNNNNNTTGG 4 cut(s) 341, 373, 380, 412
AleI CACNNNNGTG 1 cut(s) 224
AluBI AGCT 4 cut(s) 119, 259, 291, 431
AluI AGCT 4 cut(s) 119, 259, 291, 431
Alw21I GWGCWC 1 cut(s) 293
Alw26I GTCTC 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 1 cut(s) 189
ApeKI GCWGC 2 cut(s) 14, 371
ApoI RAATTY 2 cut(s) 365, 395
AvaI CYCGRG 1 cut(s) 211
BanI GGYRCC 1 cut(s) 327
BanII GRGCYC 1 cut(s) 293
Bbv12I GWGCWC 1 cut(s) 293
BbvI GCAGC 2 cut(s) 26, 358
BccI CCATC 3 cut(s) 146, 310, 479
BciVI GTATCC 2 cut(s) 193, 199
BclI TGATCA 1 cut(s) 262
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 434
BfuI GTATCC 2 cut(s) 193, 199
BisI GCNGC 4 cut(s) 15, 94, 331, 372
BlsI GCNGC 4 cut(s) 16, 95, 332, 373
BmeT110I CYCGRG 1 cut(s) 211
BmiI GGNNCC 1 cut(s) 329
BmsI GCATC 2 cut(s) 329, 348
BplI GAGNNNNNCTC 2 cut(s) 540, 572
BpmI CTGGAG 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 299
Bse3DI GCAATG 1 cut(s) 94
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 1 cut(s) 321
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 94
BseNI ACTGG 1 cut(s) 299
BseXI GCAGC 2 cut(s) 26, 358
BshFI GGCC 1 cut(s) 191
BshNI GGYRCC 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 293
BsiHKCI CYCGRG 1 cut(s) 211
BslFI GGGAC 3 cut(s) 188, 201, 528
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 1 cut(s) 57
BsmFI GGGAC 3 cut(s) 188, 201, 528
BsnI GGCC 1 cut(s) 191
Bso31I GGTCTC 1 cut(s) 57
BsoBI CYCGRG 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 293
Bsp143I GATC 2 cut(s) 262, 390
BspACI CCGC 3 cut(s) 93, 96, 331
BspANI GGCC 1 cut(s) 191
BspLI GGNNCC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 327
BspTNI GGTCTC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 94
BsrI ACTGG 1 cut(s) 299
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 262, 390
Bst4CI ACNGT 4 cut(s) 56, 78, 223, 251
Bst6I CTCTTC 1 cut(s) 164
BstDEI CTNAG 1 cut(s) 272
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 321
BstKTI GATC 2 cut(s) 265, 393
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 2 cut(s) 262, 390
BstMWI GCNNNNNNNGC 3 cut(s) 93, 109, 336
BstV1I GCAGC 2 cut(s) 26, 358
BstXI CCANNNNNNTGG 2 cut(s) 81, 226
BsuI GTATCC 2 cut(s) 193, 199
BsuRI GGCC 1 cut(s) 191
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 321
Csp6I GTAC 2 cut(s) 422, 493
CviAII CATG 2 cut(s) 278, 525
CviQI GTAC 2 cut(s) 422, 493
DdeI CTNAG 1 cut(s) 272
DpnI GATC 2 cut(s) 264, 392
DpnII GATC 2 cut(s) 262, 390
DrdI GACNNNNNNGTC 1 cut(s) 48
DseDI GACNNNNNNGTC 1 cut(s) 48
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 291
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 293
FaeI CATG 2 cut(s) 281, 528
FaiI YATR 7 cut(s) 137, 279, 381, 405, 478, 519, 526
FalI AAGNNNNNCTT 4 cut(s) 161, 193, 513, 545
FaqI GGGAC 3 cut(s) 188, 201, 528
FatI CATG 2 cut(s) 277, 524
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 4 cut(s) 15, 94, 331, 372
FokI GGATG 1 cut(s) 328
FriOI GRGCYC 1 cut(s) 293
Fsp4HI GCNGC 4 cut(s) 15, 94, 331, 372
FspBI CTAG 1 cut(s) 434
GluI GCNGC 4 cut(s) 15, 94, 331, 372
GsuI CTGGAG 1 cut(s) 103
HaeIII GGCC 1 cut(s) 191
Hin1II CATG 2 cut(s) 281, 528
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 2 cut(s) 214, 268
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 2 cut(s) 82, 123
Hpy8I GTNNAC 1 cut(s) 415
HpyCH4III ACNGT 4 cut(s) 56, 78, 223, 251
HpyCH4IV ACGT 2 cut(s) 27, 164
HpyCH4V TGCA 2 cut(s) 281, 339
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 109, 336
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 2 cut(s) 27, 164
Hsp92II CATG 2 cut(s) 281, 528
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 2 cut(s) 262, 390
LmnI GCTCC 1 cut(s) 84
LpnPI CCDG 5 cut(s) 67, 108, 269, 312, 458
Lsp1109I GCAGC 2 cut(s) 26, 358
LweI GCATC 2 cut(s) 329, 348
MaeI CTAG 1 cut(s) 434
MaeII ACGT 2 cut(s) 27, 164
MaeIII GTNAC 2 cut(s) 50, 56
MalI GATC 2 cut(s) 264, 392
MboI GATC 2 cut(s) 262, 390
MboII GAAGA 2 cut(s) 181, 551
MhlI GDGCHC 1 cut(s) 293
MluCI AATT 5 cut(s) 19, 356, 365, 395, 468
MlyI GAGTC 1 cut(s) 223
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 4 cut(s) 165, 181, 220, 435
Mph1103I ATGCAT 1 cut(s) 341
MslI CAYNNNNRTG 2 cut(s) 224, 325
MwoI GCNNNNNNNGC 3 cut(s) 93, 109, 336
NdeII GATC 2 cut(s) 262, 390
NlaIII CATG 2 cut(s) 281, 528
NlaIV GGNNCC 1 cut(s) 329
NmuCI GTSAC 2 cut(s) 50, 56
NsiI ATGCAT 1 cut(s) 341
OliI CACNNNNGTG 1 cut(s) 224
PaeR7I CTCGAG 1 cut(s) 211
PceI AGGCCT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PfeI GAWTC 1 cut(s) 268
PkrI GCNGC 4 cut(s) 16, 95, 332, 373
PleI GAGTC 1 cut(s) 222
PpsI GAGTC 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 293
Psp1406I AACGTT 1 cut(s) 164
PspN4I GGNNCC 1 cut(s) 329
PspXI VCTCGAGB 1 cut(s) 211
RsaI GTAC 2 cut(s) 423, 494
RsaNI GTAC 2 cut(s) 422, 493
RseI CAYNNNNRTG 2 cut(s) 224, 325
SacI GAGCTC 1 cut(s) 293
SatI GCNGC 4 cut(s) 15, 94, 331, 372
Sau3AI GATC 2 cut(s) 262, 390
SchI GAGTC 1 cut(s) 223
SduI GDGCHC 1 cut(s) 293
SetI ASST 9 cut(s) 30, 121, 167, 212, 261, 293, 427, 433, 477
SfaNI GCATC 2 cut(s) 329, 348
Sfr274I CTCGAG 1 cut(s) 211
SlaI CTCGAG 1 cut(s) 211
SmiMI CAYNNNNRTG 2 cut(s) 224, 325
SmlI CTYRAG 2 cut(s) 211, 426
SmoI CTYRAG 2 cut(s) 211, 426
Sse9I AATT 5 cut(s) 19, 356, 365, 395, 468
SseBI AGGCCT 1 cut(s) 191
SsiI CCGC 3 cut(s) 93, 96, 331
SspMI CTAG 1 cut(s) 434
SstI GAGCTC 1 cut(s) 293
StuI AGGCCT 1 cut(s) 191
TaaI ACNGT 4 cut(s) 56, 78, 223, 251
TaiI ACGT 2 cut(s) 30, 167
TaqI TCGA 1 cut(s) 212
TasI AATT 5 cut(s) 19, 356, 365, 395, 468
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 96, 333
TfiI GAWTC 1 cut(s) 268
TseFI GTSAC 2 cut(s) 50, 56
TseI GCWGC 2 cut(s) 14, 371
Tsp45I GTSAC 2 cut(s) 50, 56
TspGWI ACGGA 2 cut(s) 38, 218
XapI RAATTY 2 cut(s) 365, 395
XhoI CTCGAG 1 cut(s) 211
XspI CTAG 1 cut(s) 434
Zsp2I ATGCAT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.