Rroxscaffold_6G00402420

Thioredoxin-like 3-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011087
Physical Location & Seq
Forward (+)
24908194 .. 24909829
1636 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_6G00402420.1

Sequence Viewer

Length: 558 bp
ATGTCTGTTCTGGCAGCAAATTCTCACATTTTGTGTAGAGAAGTTCATCGCAGGGAGCATCAACAACAGTTTTCGAGCGCTGCAGGGGGCAGTTGTGTTTTGCCGAAATCTTCTGGGTTTGGTTTCTTCCATAGAAACAGAGATAGCAAGAAGATATTGAGAAGAGACTTGAAAGTAGAGGCCTTTTGGCCAGAATCCAGACCCACTTTTGTAGAGATGGAACCTATAAACGACTCTGATCACCTTGATCATATATTAAGCCAGGCTCAACAGCTCTCCCAGCCCATCGTAATTGATTGGATGGCAGCTTGGTGCCGCAAATGCATCTATTTGAAGCCTAAATTGGAGAAATTGGCTGCTGAATATGAAACCAAGGTCAAATTCTATTATGTAGATGTCAACAAGGTGCCTCAAACTCTAGTGAAACGCGGAAACATCTCTAAAATGCCAACCATTCAGGTGTGGAAAGATGGAGAGTTCAAGGAAGAAGTGATTGGAGGTCATAAAGCATGGCTTGTGATTGATGAAGTGCGAGAAATGATCCAAAAATTTGTATGA

Protein Analysis

185

Amino Acids

21.51

Weight (kDa)

9.02

Isoelectric Point (pI)

58.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 90 - 169 1.2e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06690 AT5G06690 AT5G06690 AT5G06690
fragaria_vesca FvH4_6g22000
malus_domestica MD12G1055400.v1.1 MD14G1054800.v1.1
prunus_persica Prupe.7G079900_v2.0.a1
pyrus_communis pycom12g04840
rosa_chinensis RchiOBHm_Chr3g0479301
rosa_laevigata RLG00000023585
rosa_multiflora Rmu_sc0026945.1_g000002
rosa_roxburghii Rroxscaffold_6G00402420
rosa_rugosa Rorug03G0174200 Rorug03G0174200
rosa_samantha Rh3AG225300 Rh3BG257800 Rh3CG253500 Rh3DG249700
rosa_wichuraiana Rw3G020150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 312, 406
AccII CGCG 1 cut(s) 429
AciI CCGC 2 cut(s) 316, 429
AclWI GGATC 1 cut(s) 535
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 3 cut(s) 19, 380, 548
AfeI AGCGCT 1 cut(s) 79
AgsI TTSAA 3 cut(s) 172, 334, 481
AjnI CCWGG 1 cut(s) 261
AjuI GAANNNNNNNTTGG 6 cut(s) 326, 358, 365, 397, 477, 509
AluBI AGCT 2 cut(s) 274, 308
AluI AGCT 2 cut(s) 274, 308
Alw26I GTCTC 1 cut(s) 159
AlwI GGATC 1 cut(s) 535
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 2 cut(s) 180, 188
ApeKI GCWGC 4 cut(s) 14, 80, 305, 356
ApoI RAATTY 3 cut(s) 19, 380, 548
AspLEI GCGC 1 cut(s) 80
AsuHPI GGTGA 1 cut(s) 233
BalI TGGCCA 1 cut(s) 190
BanI GGYRCC 2 cut(s) 312, 406
BbvI GCAGC 4 cut(s) 26, 67, 317, 343
BccI CCATC 4 cut(s) 211, 293, 295, 464
BciT130I CCWGG 1 cut(s) 263
BclI TGATCA 2 cut(s) 238, 247
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 5 cut(s) 15, 81, 306, 316, 357
BlsI GCNGC 5 cut(s) 16, 82, 307, 317, 358
Bme1390I CCNGG 1 cut(s) 263
BmiI GGNNCC 3 cut(s) 222, 314, 408
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 2 cut(s) 67, 333
BsaJI CCNNGG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 1 cut(s) 306
BseXI GCAGC 4 cut(s) 26, 67, 317, 343
BseYI CCCAGC 1 cut(s) 279
Bsh1236I CGCG 1 cut(s) 429
BshFI GGCC 2 cut(s) 182, 190
BshNI GGYRCC 2 cut(s) 312, 406
BsmAI GTCTC 1 cut(s) 159
BsnI GGCC 2 cut(s) 182, 190
Bsp143I GATC 3 cut(s) 238, 247, 540
BspACI CCGC 2 cut(s) 316, 429
BspANI GGCC 2 cut(s) 182, 190
BspFNI CGCG 1 cut(s) 429
BspLI GGNNCC 3 cut(s) 222, 314, 408
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 1 cut(s) 535
BspT107I GGYRCC 2 cut(s) 312, 406
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 3 cut(s) 238, 247, 540
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 69
Bst6I CTCTTC 1 cut(s) 157
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 429
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 3 cut(s) 241, 250, 543
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 3 cut(s) 238, 247, 540
BstMWI GCNNNNNNNGC 2 cut(s) 280, 321
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 81
BstUI CGCG 1 cut(s) 429
BstV1I GCAGC 4 cut(s) 26, 67, 317, 343
BsuRI GGCC 2 cut(s) 182, 190
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 1 cut(s) 306
CfoI GCGC 1 cut(s) 80
CviAII CATG 1 cut(s) 510
DpnI GATC 3 cut(s) 240, 249, 542
DpnII GATC 3 cut(s) 238, 247, 540
EaeI YGGCCR 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 182
Eco47III AGCGCT 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 1 cut(s) 513
FaiI YATR 9 cut(s) 132, 227, 252, 254, 366, 390, 504, 511, 556
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FatI CATG 1 cut(s) 509
FbaI TGATCA 2 cut(s) 238, 247
Fnu4HI GCNGC 5 cut(s) 15, 81, 306, 316, 357
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 15, 81, 306, 316, 357
FspBI CTAG 1 cut(s) 419
GlaI GCGC 1 cut(s) 79
GluI GCNGC 5 cut(s) 15, 81, 306, 316, 357
GsaI CCCAGC 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 2 cut(s) 182, 190
HhaI GCGC 1 cut(s) 80
Hin1II CATG 1 cut(s) 513
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 2 cut(s) 194, 233
HphI GGTGA 1 cut(s) 233
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 1 cut(s) 400
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4V TGCA 2 cut(s) 83, 324
HpyF10VI GCNNNNNNNGC 2 cut(s) 280, 321
Hsp92II CATG 1 cut(s) 513
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 238, 247
Kzo9I GATC 3 cut(s) 238, 247, 540
LmnI GCTCC 1 cut(s) 55
LpnPI CCDG 9 cut(s) 37, 69, 99, 204, 211, 248, 275, 293, 443
Lsp1109I GCAGC 4 cut(s) 26, 67, 317, 343
LweI GCATC 2 cut(s) 67, 333
MaeI CTAG 1 cut(s) 419
MalI GATC 3 cut(s) 240, 249, 542
MboI GATC 3 cut(s) 238, 247, 540
MboII GAAGA 5 cut(s) 102, 118, 163, 174, 497
MlsI TGGCCA 1 cut(s) 190
MluCI AATT 6 cut(s) 19, 291, 341, 350, 380, 548
MluNI TGGCCA 1 cut(s) 190
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 3 cut(s) 172, 420, 491
Mox20I TGGCCA 1 cut(s) 190
Mph1103I ATGCAT 1 cut(s) 326
MscI TGGCCA 1 cut(s) 190
MseI TTAA 1 cut(s) 257
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 190
MspR9I CCNGG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 429
MwoI GCNNNNNNNGC 2 cut(s) 280, 321
NdeII GATC 3 cut(s) 238, 247, 540
NlaIII CATG 1 cut(s) 513
NlaIV GGNNCC 3 cut(s) 222, 314, 408
NsiI ATGCAT 1 cut(s) 326
PceI AGGCCT 1 cut(s) 182
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 5 cut(s) 16, 82, 307, 317, 358
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
Psp6I CCWGG 1 cut(s) 261
PspFI CCCAGC 1 cut(s) 279
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 3 cut(s) 222, 314, 408
PstI CTGCAG 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 5 cut(s) 15, 81, 306, 316, 357
Sau3AI GATC 3 cut(s) 238, 247, 540
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 263
SetI ASST 8 cut(s) 226, 246, 276, 310, 378, 408, 462, 502
SfaNI GCATC 2 cut(s) 67, 333
SfcI CTRYAG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 458
Sse9I AATT 6 cut(s) 19, 291, 341, 350, 380, 548
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 2 cut(s) 316, 429
SspMI CTAG 1 cut(s) 419
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 74
TasI AATT 6 cut(s) 19, 291, 341, 350, 380, 548
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TseI GCWGC 4 cut(s) 14, 80, 305, 356
TspDTI ATGAA 3 cut(s) 35, 381, 540
XapI RAATTY 3 cut(s) 19, 380, 548
XspI CTAG 1 cut(s) 419
Zsp2I ATGCAT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.