pycom12g04840

Thioredoxin-like 3-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011087
Physical Location & Seq
Reverse (-)
4752880 .. 4754510
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04840.1

Sequence Viewer

Length: 567 bp
ATGTCTGTTTTGGCAGCCAATTCTCATGTCCTGTGTAGCAGAGAAGTCCATCACAGAGAGCAACAGCCACAGTTCTGGAGCAACGGCGGCGGTTTCTTGCTGTTGCCAAAAGCTTCCGGATTAGGGTTTTTGAACAGAAACAGAGATGGGAACAGGACGTTGAAGATGGACTTGAAAGTTGAGGCCTTTTGGGGCACGGATACCTCGAGACCCACAGTTGTGGAGTTGGAAGCAATCAACGACTGTGATCAGCTTGACCAGATTCTCAGCCAGGCACAGGAGAGATCCCAGCCCATTGTCATTGATTGGATGGCATCTTGGTGCCGCAAATGCATATATTTGAAGCCTAAATTGGAGAAGTTGGCTGCTGAATATGACACCAAGGTCAAATTCTACTACGTTGATGTCAACAAAGTACCTCAAGCTCTAGTAAAGCGGGGAAACATCTCTAAAATGCCAACAATTCAGGTATGGAAAGATGGAGAGTTTAAAGCCGAAGTGATTGGGGGACATAAAGCATGGCTTGTGCTTGAAGAAGTGAGAGAAATGCTCCAAAACTTTGTGTGA

Protein Analysis

189

Amino Acids

21.56

Weight (kDa)

8.31

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 93 - 172 1.5e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G06690 AT5G06690 AT5G06690 AT5G06690
fragaria_vesca FvH4_6g22000
malus_domestica MD12G1055400.v1.1 MD14G1054800.v1.1
prunus_persica Prupe.7G079900_v2.0.a1
pyrus_communis pycom12g04840
rosa_chinensis RchiOBHm_Chr3g0479301
rosa_laevigata RLG00000023585
rosa_multiflora Rmu_sc0026945.1_g000002
rosa_roxburghii Rroxscaffold_6G00402420
rosa_rugosa Rorug03G0174200 Rorug03G0174200
rosa_samantha Rh3AG225300 Rh3BG257800 Rh3CG253500 Rh3DG249700
rosa_wichuraiana Rw3G020150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 383
AccB1I GGYRCC 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 116
AciI CCGC 4 cut(s) 87, 90, 325, 436
AclWI GGATC 1 cut(s) 279
AcsI RAATTY 1 cut(s) 389
AfaI GTAC 1 cut(s) 417
AfiI CCNNNNNNNGG 2 cut(s) 123, 277
AgsI TTSAA 5 cut(s) 133, 163, 175, 343, 533
AjnI CCWGG 1 cut(s) 270
AjuI GAANNNNNNNTTGG 4 cut(s) 335, 367, 374, 406
AleI CACNNNNGTG 1 cut(s) 218
AluBI AGCT 3 cut(s) 113, 253, 425
AluI AGCT 3 cut(s) 113, 253, 425
Alw26I GTCTC 1 cut(s) 202
AlwI GGATC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 116
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 2 cut(s) 14, 365
ApoI RAATTY 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 197
BanI GGYRCC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 26, 352
BccI CCATC 5 cut(s) 57, 140, 160, 304, 473
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 1 cut(s) 272
BciVI GTATCC 1 cut(s) 193
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 202
BfaI CTAG 1 cut(s) 428
BfuI GTATCC 1 cut(s) 193
BisI GCNGC 4 cut(s) 15, 88, 325, 366
BlsI GCNGC 4 cut(s) 16, 89, 326, 367
Bme1390I CCNGG 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 205
BmiI GGNNCC 1 cut(s) 323
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 1 cut(s) 323
BplI GAGNNNNNCTC 2 cut(s) 534, 566
BpmI CTGGAG 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 381
BsaWI WCCGGW 1 cut(s) 116
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 277
BseAI TCCGGA 1 cut(s) 116
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 123, 277
BseMII CTCAG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 2 cut(s) 26, 352
BseYI CCCAGC 1 cut(s) 288
BshFI GGCC 1 cut(s) 185
BshNI GGYRCC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 205
BsiSI CCGG 1 cut(s) 117
BslFI GGGAC 1 cut(s) 522
BslI CCNNNNNNNGG 2 cut(s) 123, 277
BsmAI GTCTC 1 cut(s) 202
BsmFI GGGAC 1 cut(s) 522
BsnI GGCC 1 cut(s) 185
Bso31I GGTCTC 1 cut(s) 202
BsoBI CYCGRG 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 197
Bsp13I TCCGGA 1 cut(s) 116
Bsp143I GATC 2 cut(s) 247, 284
BspACI CCGC 4 cut(s) 87, 90, 325, 436
BspANI GGCC 1 cut(s) 185
BspCNI CTCAG 1 cut(s) 279
BspEI TCCGGA 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 323
BspPI GGATC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 202
BssECI CCNNGG 1 cut(s) 381
BssMI GATC 2 cut(s) 247, 284
BssT1I CCWWGG 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 3 cut(s) 72, 217, 245
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 315
BstKTI GATC 2 cut(s) 250, 287
BstMAI GTCTC 1 cut(s) 202
BstMBI GATC 2 cut(s) 247, 284
BstMWI GCNNNNNNNGC 2 cut(s) 87, 330
BstNI CCWGG 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 197
BstV1I GCAGC 2 cut(s) 26, 352
BstX2I RGATCY 1 cut(s) 284
BstXI CCANNNNNNTGG 2 cut(s) 75, 220
BstYI RGATCY 1 cut(s) 284
BsuI GTATCC 1 cut(s) 193
BsuRI GGCC 1 cut(s) 185
BtsCI GGATG 1 cut(s) 315
Csp6I GTAC 1 cut(s) 416
CviAII CATG 2 cut(s) 26, 519
CviQI GTAC 1 cut(s) 416
DdeI CTNAG 1 cut(s) 266
DpnI GATC 2 cut(s) 249, 286
DpnII GATC 2 cut(s) 247, 284
DraI TTTAAA 1 cut(s) 490
DrdI GACNNNNNNGTC 1 cut(s) 383
DseDI GACNNNNNNGTC 1 cut(s) 383
Eco130I CCWWGG 1 cut(s) 381
Eco147I AGGCCT 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 270
EcoT14I CCWWGG 1 cut(s) 381
EcoT22I ATGCAT 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 2 cut(s) 29, 522
FaiI YATR 7 cut(s) 27, 335, 337, 375, 472, 513, 520
FalI AAGNNNNNCTT 4 cut(s) 155, 187, 507, 539
FaqI GGGAC 1 cut(s) 522
FatI CATG 2 cut(s) 25, 518
FauI CCCGC 1 cut(s) 429
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 4 cut(s) 15, 88, 325, 366
FokI GGATG 1 cut(s) 322
Fsp4HI GCNGC 4 cut(s) 15, 88, 325, 366
FspBI CTAG 1 cut(s) 428
GluI GCNGC 4 cut(s) 15, 88, 325, 366
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 1 cut(s) 185
HapII CCGG 1 cut(s) 117
Hin1II CATG 2 cut(s) 29, 522
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 1 cut(s) 262
HpaII CCGG 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 409
Hpy188III TCNNGA 3 cut(s) 76, 117, 207
Hpy8I GTNNAC 1 cut(s) 409
HpyCH4III ACNGT 3 cut(s) 72, 217, 245
HpyCH4IV ACGT 2 cut(s) 158, 399
HpyCH4V TGCA 1 cut(s) 333
HpyF10VI GCNNNNNNNGC 2 cut(s) 87, 330
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 2 cut(s) 158, 399
Hsp92II CATG 2 cut(s) 29, 522
Kpn2I TCCGGA 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 247, 284
LmnI GCTCC 2 cut(s) 78, 555
Lsp1109I GCAGC 2 cut(s) 26, 352
LweI GCATC 1 cut(s) 323
MaeI CTAG 1 cut(s) 428
MaeII ACGT 2 cut(s) 158, 399
MalI GATC 2 cut(s) 249, 286
MboI GATC 2 cut(s) 247, 284
MboII GAAGA 2 cut(s) 175, 545
MflI RGATCY 1 cut(s) 284
MhlI GDGCHC 1 cut(s) 197
MluCI AATT 4 cut(s) 19, 350, 389, 462
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 3 cut(s) 175, 214, 429
Mph1103I ATGCAT 1 cut(s) 335
MroI TCCGGA 1 cut(s) 116
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 2 cut(s) 218, 319
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 272
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 87, 330
NdeII GATC 2 cut(s) 247, 284
NlaIII CATG 2 cut(s) 29, 522
NlaIV GGNNCC 1 cut(s) 323
NsiI ATGCAT 1 cut(s) 335
OliI CACNNNNGTG 1 cut(s) 218
PaeR7I CTCGAG 1 cut(s) 205
PceI AGGCCT 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 4 cut(s) 16, 89, 326, 367
Psp6I CCWGG 1 cut(s) 270
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 323
PsuI RGATCY 1 cut(s) 284
RsaI GTAC 1 cut(s) 417
RsaNI GTAC 1 cut(s) 416
RseI CAYNNNNRTG 2 cut(s) 218, 319
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 4 cut(s) 15, 88, 325, 366
Sau3AI GATC 2 cut(s) 247, 284
ScrFI CCNGG 1 cut(s) 272
SduI GDGCHC 1 cut(s) 197
SetI ASST 9 cut(s) 115, 161, 206, 255, 387, 402, 421, 427, 471
SfaNI GCATC 1 cut(s) 323
Sfr274I CTCGAG 1 cut(s) 205
SlaI CTCGAG 1 cut(s) 205
SmiMI CAYNNNNRTG 2 cut(s) 218, 319
SmlI CTYRAG 2 cut(s) 205, 420
SmoI CTYRAG 2 cut(s) 205, 420
Sse9I AATT 4 cut(s) 19, 350, 389, 462
SseBI AGGCCT 1 cut(s) 185
SsiI CCGC 4 cut(s) 87, 90, 325, 436
SspMI CTAG 1 cut(s) 428
StuI AGGCCT 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 270
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 3 cut(s) 72, 217, 245
TaiI ACGT 2 cut(s) 161, 402
TaqI TCGA 1 cut(s) 206
TasI AATT 4 cut(s) 19, 350, 389, 462
TauI GCSGC 2 cut(s) 90, 327
TfiI GAWTC 1 cut(s) 262
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseI GCWGC 2 cut(s) 14, 365
TspGWI ACGGA 1 cut(s) 212
XapI RAATTY 1 cut(s) 389
XhoI CTCGAG 1 cut(s) 205
XspI CTAG 1 cut(s) 428
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.