FvH4_6g25100

Cysteine-rich TM module stress tolerance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0010151
Physical Location & Seq
Forward (+)
19052159 .. 19053549
1391 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g25100.t1

Sequence Viewer

Length: 345 bp
ATGGAGGCACTTGCTTTTAGCGCTTGTGAGTTGGTTCTTCATTTACGGTTTGTGAATGTTGCATTCGAGACTGATATCGTTTATCTAGTTGTTCAAGTACTCAGCATACACAATAATACTACAGTCACCAAGGCAAGTCTTAGCTCCAAGTGTTTCTTCATCAAAATGGCCAAGACCACAGAGACACAAGACAACCAACCTCCACCAGGATACCCAACTGCAACCAATGATCCTCCAACTAGGAAGAAGTTTCGGCCCCAGACTAAAAAGAAAGGAGACAGGGGCTTCATTGAGGGCTGCTTATTTGCCTTGTGCTGCTGTTGGCTCTGCGAGGAATGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

115

Amino Acids

12.84

Weight (kDa)

8.12

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 69 - 114 3.6e-15 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 168
AfaI GTAC 1 cut(s) 99
AfeI AGCGCT 1 cut(s) 22
AfiI CCNNNNNNNGG 1 cut(s) 206
AgsI TTSAA 1 cut(s) 95
AjnI CCWGG 1 cut(s) 205
AjuI GAANNNNNNNTTGG 2 cut(s) 140, 172
AluBI AGCT 1 cut(s) 144
AluI AGCT 1 cut(s) 144
Alw26I GTCTC 3 cut(s) 62, 176, 270
AlwI GGATC 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 22
AoxI GGCC 2 cut(s) 168, 254
ApeKI GCWGC 2 cut(s) 297, 315
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 118
BalI TGGCCA 1 cut(s) 170
BbvI GCAGC 2 cut(s) 284, 302
BciT130I CCWGG 1 cut(s) 207
BciVI GTATCC 1 cut(s) 203
BcoDI GTCTC 3 cut(s) 62, 176, 270
BfaI CTAG 2 cut(s) 86, 240
BfmI CTRYAG 1 cut(s) 120
BfoI RGCGCY 1 cut(s) 24
BfuI GTATCC 1 cut(s) 203
BisI GCNGC 2 cut(s) 298, 316
BlsI GCNGC 2 cut(s) 299, 317
BmcAI AGTACT 1 cut(s) 99
Bme1390I CCNGG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 255
BmiI GGNNCC 1 cut(s) 257
BmrFI CCNGG 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 129
Bsc4I CCNNNNNNNGG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 129
BseLI CCNNNNNNNGG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 115
BseXI GCAGC 2 cut(s) 284, 302
BshFI GGCC 2 cut(s) 170, 256
BslI CCNNNNNNNGG 1 cut(s) 206
BsmAI GTCTC 3 cut(s) 62, 176, 270
BsmI GAATGC 1 cut(s) 62
BsnI GGCC 2 cut(s) 170, 256
Bsp143I GATC 1 cut(s) 229
BspANI GGCC 2 cut(s) 170, 256
BspCNI CTCAG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 257
BspPI GGATC 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 1 cut(s) 229
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 48, 124
BstDEI CTNAG 2 cut(s) 101, 140
BstENI CCTNNNNNAGG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 3 cut(s) 62, 176, 270
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 284, 302
BsuI GTATCC 1 cut(s) 203
BsuRI GGCC 2 cut(s) 170, 256
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 255
Csp6I GTAC 1 cut(s) 98
CviJI RGCY 6 cut(s) 144, 170, 256, 285, 297, 325
CviKI_1 RGCY 6 cut(s) 144, 170, 256, 285, 297, 325
CviQI GTAC 1 cut(s) 98
DdeI CTNAG 2 cut(s) 101, 140
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 168
Eco130I CCWWGG 1 cut(s) 129
Eco32I GATATC 1 cut(s) 76
Eco47III AGCGCT 1 cut(s) 22
EcoNI CCTNNNNNAGG 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 205
EcoRV GATATC 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaiI YATR 1 cut(s) 107
FalI AAGNNNNNCTT 2 cut(s) 140, 172
Fnu4HI GCNGC 2 cut(s) 298, 316
Fsp4HI GCNGC 2 cut(s) 298, 316
FspBI CTAG 2 cut(s) 86, 240
GlaI GCGC 1 cut(s) 22
GluI GCNGC 2 cut(s) 298, 316
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 2 cut(s) 170, 256
HhaI GCGC 1 cut(s) 23
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HphI GGTGA 1 cut(s) 118
Hpy188I TCNGA 1 cut(s) 344
Hpy188III TCNNGA 1 cut(s) 67
HpyCH4III ACNGT 2 cut(s) 48, 124
HpyCH4V TGCA 2 cut(s) 62, 221
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 2 cut(s) 101, 140
HspAI GCGC 1 cut(s) 21
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 1 cut(s) 149
LpnPI CCDG 4 cut(s) 192, 219, 265, 272
Lsp1109I GCAGC 2 cut(s) 284, 302
MaeI CTAG 2 cut(s) 86, 240
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 3 cut(s) 29, 148, 256
MlsI TGGCCA 1 cut(s) 170
MluNI TGGCCA 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 4 cut(s) 210, 243, 286, 325
Mox20I TGGCCA 1 cut(s) 170
MscI TGGCCA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 170
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 62
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 20
NdeII GATC 1 cut(s) 229
NlaIV GGNNCC 1 cut(s) 257
NmuCI GTSAC 1 cut(s) 124
PctI GAATGC 1 cut(s) 62
PkrI GCNGC 2 cut(s) 299, 317
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 257
PspPI GGNCC 1 cut(s) 255
RsaI GTAC 1 cut(s) 99
RsaNI GTAC 1 cut(s) 98
RseI CAYNNNNRTG 1 cut(s) 164
SatI GCNGC 2 cut(s) 298, 316
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 1 cut(s) 255
ScaI AGTACT 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 207
SetI ASST 2 cut(s) 146, 202
SfcI CTRYAG 1 cut(s) 120
SmiMI CAYNNNNRTG 1 cut(s) 164
SspMI CTAG 2 cut(s) 86, 240
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 48, 124
TaqI TCGA 1 cut(s) 66
TatI WGTACW 1 cut(s) 97
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 297, 315
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 3 cut(s) 29, 148, 277
XagI CCTNNNNNAGG 1 cut(s) 204
XspI CTAG 2 cut(s) 86, 240
ZrmI AGTACT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.