MD12G1091200.v1.1

Cysteine-rich TM module stress tolerance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010151
Physical Location & Seq
Forward (+)
11536135 .. 11537087
953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1091200.v1.1.491

Sequence Viewer

Length: 177 bp
ATGGCCAAGACAGAGACACAAGAAACCCAACCTCCATCAGGATACCCAACTGAAACCGAGAATCCCCCAACTGGGAAGAAGTTTAGACCATGGACAAAAAAGAAAGGAGACAGAGGCTTCATTGAGGGATGCCTCTTCGCCTTATGCTGCTGCTGGCTATGTGAGGAATGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

59

Amino Acids

6.62

Weight (kDa)

6.17

Isoelectric Point (pI)

59.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 14 - 58 1.8e-11 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 3
AfiI CCNNNNNNNGG 3 cut(s) 38, 71, 72
Alw26I GTCTC 2 cut(s) 8, 102
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 147, 150
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 2 cut(s) 134, 137
BccI CCATC 1 cut(s) 43
BciVI GTATCC 1 cut(s) 35
BcoDI GTCTC 2 cut(s) 8, 102
BfuI GTATCC 1 cut(s) 35
BisI GCNGC 2 cut(s) 148, 151
BlsI GCNGC 2 cut(s) 149, 152
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 1 cut(s) 119
BmuI ACTGGG 1 cut(s) 81
BsaJI CCNNGG 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 16, 46
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 71, 72
Bse1I ACTGG 1 cut(s) 76
BseDI CCNNGG 1 cut(s) 89
BseGI GGATG 1 cut(s) 134
BseLI CCNNNNNNNGG 3 cut(s) 38, 71, 72
BseNI ACTGG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 134, 137
BshFI GGCC 1 cut(s) 5
BslI CCNNNNNNNGG 3 cut(s) 38, 71, 72
BsmAI GTCTC 2 cut(s) 8, 102
BsmI GAATGC 1 cut(s) 173
BsnI GGCC 1 cut(s) 5
Bsp19I CCATGG 1 cut(s) 89
BspANI GGCC 1 cut(s) 5
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 155
BstDSI CCRYGG 1 cut(s) 89
BstENI CCTNNNNNAGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 134
BstMAI GTCTC 2 cut(s) 8, 102
BstV1I GCAGC 2 cut(s) 134, 137
BsuI GTATCC 1 cut(s) 35
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 155
CviAII CATG 1 cut(s) 90
CviJI RGCY 3 cut(s) 5, 117, 157
CviKI_1 RGCY 3 cut(s) 5, 117, 157
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 1 cut(s) 89
EcoNI CCTNNNNNAGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 1 cut(s) 93
FaiI YATR 3 cut(s) 91, 145, 160
FatI CATG 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 148, 151
FokI GGATG 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 148, 151
GluI GCNGC 2 cut(s) 148, 151
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 1 cut(s) 93
HinfI GANTC 1 cut(s) 61
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 1 cut(s) 39
Hsp92II CATG 1 cut(s) 93
LpnPI CCDG 3 cut(s) 24, 57, 139
Lsp1109I GCAGC 2 cut(s) 134, 137
LweI GCATC 1 cut(s) 119
MboII GAAGA 2 cut(s) 88, 127
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 5 cut(s) 42, 107, 118, 143, 157
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
Mva1269I GAATGC 1 cut(s) 173
NcoI CCATGG 1 cut(s) 89
NlaIII CATG 1 cut(s) 93
PctI GAATGC 1 cut(s) 173
PfeI GAWTC 1 cut(s) 61
PkrI GCNGC 2 cut(s) 149, 152
SatI GCNGC 2 cut(s) 148, 151
SetI ASST 1 cut(s) 34
SfaNI GCATC 1 cut(s) 119
SgeI CNNG 7 cut(s) 19, 32, 51, 70, 84, 102, 166
StyI CCWWGG 1 cut(s) 89
TfiI GAWTC 1 cut(s) 61
TseI GCWGC 2 cut(s) 147, 150
TspDTI ATGAA 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.