FvH4_6g26520

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006101
Physical Location & Seq
Reverse (-)
20263308 .. 20268102
4795 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26520.t7

Sequence Viewer

Length: 1587 bp
ATGGCTGCCGTCGAAGACCCTCTCCTGTCCTCCCTCTGGTCGATAAAGGAAGCGCTGGATGACTTTAACTCCGGGAAGGATTCCGGGTCGGATCTCTCTAGGCTGCTATCCGATTGCATCGCAGCTTTCAAGCACGATCCTCACTACCGAGACGACACCAGATTCCTCAAGATTTGGTTCCTCTATGTAATGGGATTCATCCAAGATTACGAAACCGTGTTTCTGGAAATGCTGGATAGGAAGATATGTATTGGGCAGTCCTTGCTCTATGTTTGGTATGCCTCCTTTCTAGAATCCAAAGGCAAATTGTACGATGCGCAAATGGTTTATCACTTCGGTATTTCAAGCAATGCTAAGCCTGCTGAGTGGGTGACCAAAGCGCATGCCTTTTTTCTTGAGAGGATGTCTGAATTAGTGTCTTCAACTCACGAGGTTGACAACGGTCAATGCATTCAGTTTGAGAGCCACATCAATCCATGGTCCAATTCCACAATGAAAGCCCTTTTAAAGAAAATCACTCCTCGACTAGCGAAATTTGATGGATATCATGCCACTAATAAAGCTTATTCAGGAAAAGTGGCATTGTCTTCTTTGAAGAAATCATCAAGGAACAAGACTATTGAGATAGGTCGTACGAAATACCAGATCACGGGTTGTGCAGGTCAAGGTGCTTTTGCTGAAGTTTATAAAGCATATGTCAACTGTAACCCTGATGATGTTGTTGCATTAAAGATACAAAAGCCTGCTTTCCCGTGGGAATTCTATATGTACCGTGAACTTGATGAACGAATCTCAGACAAGGAAAGGTCAAGCTTTGGTTTCGCTCAAAGAATGAATCACTATTCTGACTGTAGTATTCTCGTCTGCGACTATATTGCTAATGGGACACTTCAGGATGTAATAAACTCATTTGCAGTCACCGGAAAATCCATGGAAGAAGAGTTATGCATTTATTACACCATAGAAATGCTCTACATGTTAGAAACTCTGCATGATGTTGACATCATTCATGGAGATTTCAAGCCTGATAATCTGCTTATTCGCTATTCTCGGGACTTCCTCACAAAAAGTGGATTCCATGACAGAAATGAGCTTACAGAAGATGGATTTCGGGATAGAAGTGGCCCCTGGCTTGATCAGGGTCTTTGCCTTGTGGACTGGGGAAGAGGGATAGATCTGCGTCTCTTTCCTGAAAATATAGAGTTTAAGGGAGATTGCCGAACTTCTGGATTTCGTTGTGTGGAGATGCAAGAGAATAAGCCTTGGAAATTTCAGGTAGACACGTATGGACTCTGTGTCGTTGTTCATATGATGCTGCATAGTTCTTACATGGAGATTTACAAGAAACCATCACCTGATGGTGGTTATGTTTATCAACCCAAGGCATCTTTTAAAAGATACTGGAAAGTTGAACTCTGGAAGAATTTCTTTGTGAAACTGCTTAACAGTAATCCTGGCTGCAATAACAAGAAATTGTTGCAAAATCTGAGGGAGTCTTTCCAGGAATATATCTGCTCGGACCCCCACCTTCTAAAGAAACTAAGTGATTTACTGGCAAAGCAAAGGAAATCAATGTGTTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

529

Amino Acids

61.09

Weight (kDa)

6.71

Isoelectric Point (pI)

29.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 153 - 378 3.4e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
Acc16I TGCGCA 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 650
AccI GTMKAC 1 cut(s) 1278
AclWI GGATC 2 cut(s) 99, 131
AcsI RAATTY 4 cut(s) 533, 758, 1268, 1423
AcuI CTGAAG 2 cut(s) 699, 875
AfaI GTAC 3 cut(s) 311, 634, 770
AfeI AGCGCT 1 cut(s) 54
AfiI CCNNNNNNNGG 3 cut(s) 36, 649, 1361
AflIII ACRYGT 2 cut(s) 975, 1281
AgsI TTSAA 6 cut(s) 130, 345, 423, 595, 1021, 1412
AhdI GACNNNNNGTC 1 cut(s) 1295
AjnI CCWGG 3 cut(s) 1127, 1453, 1500
AluBI AGCT 4 cut(s) 125, 563, 813, 1093
AluI AGCT 4 cut(s) 125, 563, 813, 1093
Alw26I GTCTC 2 cut(s) 144, 1187
AlwI GGATC 2 cut(s) 99, 131
Ama87I CYCGRG 1 cut(s) 1050
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 1 cut(s) 1123
ApeKI GCWGC 5 cut(s) 5, 103, 122, 1315, 1458
ApoI RAATTY 4 cut(s) 533, 758, 1268, 1423
Asp700I GAANNNNTTC 1 cut(s) 1424
AspLEI GCGC 3 cut(s) 55, 319, 382
AspS9I GGNCC 3 cut(s) 480, 1124, 1519
AsuC2I CCSGG 2 cut(s) 73, 85
AsuHPI GGTGA 3 cut(s) 382, 910, 1344
AvaI CYCGRG 1 cut(s) 1050
AvaII GGWCC 2 cut(s) 480, 1519
BauI CACGAG 1 cut(s) 428
BbsI GAAGAC 3 cut(s) 21, 411, 579
BbvI GCAGC 4 cut(s) 90, 134, 1302, 1445
BccI CCATC 4 cut(s) 533, 1097, 1352, 1357
BcgI CGANNNNNNTGC 2 cut(s) 857, 891
BciT130I CCWGG 3 cut(s) 1129, 1455, 1502
BclI TGATCA 1 cut(s) 1135
BcnI CCSGG 2 cut(s) 73, 85
BcoDI GTCTC 2 cut(s) 144, 1187
BfaI CTAG 3 cut(s) 99, 290, 527
BfmI CTRYAG 1 cut(s) 850
BfoI RGCGCY 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 650
BglII AGATCT 1 cut(s) 1174
BisI GCNGC 5 cut(s) 6, 104, 123, 1316, 1459
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 5 cut(s) 7, 105, 124, 1317, 1460
Bme1390I CCNGG 5 cut(s) 73, 85, 1129, 1455, 1502
Bme18I GGWCC 2 cut(s) 480, 1519
BmeRI GACNNNNNGTC 1 cut(s) 1295
BmeT110I CYCGRG 1 cut(s) 1050
BmgT120I GGNCC 3 cut(s) 480, 1124, 1519
BmiI GGNNCC 3 cut(s) 179, 1126, 1521
BmrFI CCNGG 5 cut(s) 73, 85, 1129, 1455, 1502
BmrI ACTGGG 1 cut(s) 1168
BmsI GCATC 5 cut(s) 126, 304, 1236, 1302, 1394
BmuI ACTGGG 1 cut(s) 1168
BoxI GACNNNNGTC 1 cut(s) 441
BpiI GAAGAC 3 cut(s) 21, 411, 579
Bpu1102I GCTNAGC 1 cut(s) 354
BpuEI CTTGAG 2 cut(s) 152, 416
BpuMI CCSGG 2 cut(s) 73, 85
BsaAI YACGTR 1 cut(s) 1284
BsaBI GATNNNNATC 1 cut(s) 543
BsaJI CCNNGG 6 cut(s) 476, 752, 930, 1127, 1262, 1380
BsaWI WCCGGW 1 cut(s) 920
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 649, 1361
Bse1I ACTGG 3 cut(s) 1163, 1406, 1557
Bse3DI GCAATG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 543
BseBI CCWGG 3 cut(s) 1129, 1455, 1502
BseDI CCNNGG 6 cut(s) 476, 752, 930, 1127, 1262, 1380
BseGI GGATG 4 cut(s) 64, 198, 408, 901
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 3 cut(s) 36, 649, 1361
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 3 cut(s) 354, 807, 1478
BseNI ACTGG 3 cut(s) 1163, 1406, 1557
BseRI GAGGAG 1 cut(s) 510
BseXI GCAGC 4 cut(s) 90, 134, 1302, 1445
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 1 cut(s) 1125
BsiHKCI CYCGRG 1 cut(s) 1050
BsiSI CCGG 3 cut(s) 72, 84, 921
BsiWI CGTACG 1 cut(s) 632
BslFI GGGAC 2 cut(s) 898, 1067
BslI CCNNNNNNNGG 3 cut(s) 36, 649, 1361
BsmAI GTCTC 2 cut(s) 144, 1187
BsmBI CGTCTC 2 cut(s) 144, 1187
BsmFI GGGAC 2 cut(s) 898, 1067
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 1 cut(s) 1125
BsoBI CYCGRG 1 cut(s) 1050
Bsp143I GATC 5 cut(s) 91, 136, 645, 1135, 1174
Bsp1720I GCTNAGC 1 cut(s) 354
Bsp19I CCATGG 2 cut(s) 476, 930
BspANI GGCC 1 cut(s) 1125
BspCNI CTCAG 3 cut(s) 355, 806, 1479
BspLI GGNNCC 3 cut(s) 179, 1126, 1521
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 2 cut(s) 99, 131
BsrDI GCAATG 1 cut(s) 355
BsrI ACTGG 3 cut(s) 1163, 1406, 1557
BssECI CCNNGG 6 cut(s) 476, 752, 930, 1127, 1262, 1380
BssMI GATC 5 cut(s) 91, 136, 645, 1135, 1174
BssSI CACGAG 1 cut(s) 428
BssT1I CCWWGG 4 cut(s) 476, 930, 1262, 1380
Bst2BI CACGAG 1 cut(s) 428
Bst2UI CCWGG 3 cut(s) 1129, 1455, 1502
Bst4CI ACNGT 6 cut(s) 217, 443, 704, 773, 851, 1448
Bst6I CTCTTC 2 cut(s) 933, 1159
BstAPI GCANNNNNTGC 1 cut(s) 262
BstBAI YACGTR 1 cut(s) 1284
BstC8I GCNNGC 3 cut(s) 360, 384, 744
BstDEI CTNAG 6 cut(s) 354, 363, 793, 1487, 1541, 1584
BstDSI CCRYGG 3 cut(s) 476, 752, 930
BstEII GGTNACC 1 cut(s) 370
BstF5I GGATG 4 cut(s) 64, 198, 408, 901
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 3 cut(s) 55, 319, 382
BstKTI GATC 5 cut(s) 94, 139, 648, 1138, 1177
BstMAI GTCTC 2 cut(s) 144, 1187
BstMBI GATC 5 cut(s) 91, 136, 645, 1135, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 262, 359
BstNI CCWGG 3 cut(s) 1129, 1455, 1502
BstNSI RCATGY 2 cut(s) 386, 979
BstPAI GACNNNNGTC 1 cut(s) 441
BstPI GGTNACC 1 cut(s) 370
BstSCI CCNGG 5 cut(s) 71, 83, 1127, 1453, 1500
BstSFI CTRYAG 1 cut(s) 850
BstV1I GCAGC 4 cut(s) 90, 134, 1302, 1445
BstV2I GAAGAC 3 cut(s) 21, 411, 579
BstX2I RGATCY 2 cut(s) 91, 1174
BstYI RGATCY 2 cut(s) 91, 1174
BsuRI GGCC 1 cut(s) 1125
BtgI CCRYGG 3 cut(s) 476, 752, 930
BtgZI GCGATG 1 cut(s) 103
BtsCI GGATG 4 cut(s) 64, 198, 408, 901
BveI ACCTGC 1 cut(s) 650
Cac8I GCNNGC 3 cut(s) 360, 384, 744
CfoI GCGC 3 cut(s) 55, 319, 382
Cfr13I GGNCC 3 cut(s) 480, 1124, 1519
CseI GACGC 1 cut(s) 1169
Csp6I GTAC 3 cut(s) 310, 633, 769
CviAII CATG 9 cut(s) 383, 477, 548, 931, 976, 992, 1010, 1079, 1330
CviQI GTAC 3 cut(s) 310, 633, 769
DdeI CTNAG 6 cut(s) 354, 363, 793, 1487, 1541, 1584
DpnI GATC 5 cut(s) 93, 138, 647, 1137, 1176
DpnII GATC 5 cut(s) 91, 136, 645, 1135, 1174
DraI TTTAAA 2 cut(s) 507, 1393
DriI GACNNNNNGTC 1 cut(s) 1295
Eam1104I CTCTTC 2 cut(s) 933, 1159
Eam1105I GACNNNNNGTC 1 cut(s) 1295
EarI CTCTTC 2 cut(s) 933, 1159
Eco130I CCWWGG 4 cut(s) 476, 930, 1262, 1380
Eco32I GATATC 1 cut(s) 545
Eco47I GGWCC 2 cut(s) 480, 1519
Eco47III AGCGCT 1 cut(s) 54
Eco57I CTGAAG 2 cut(s) 699, 875
Eco88I CYCGRG 1 cut(s) 1050
Eco91I GGTNACC 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 758
EcoRII CCWGG 3 cut(s) 1127, 1453, 1500
EcoRV GATATC 1 cut(s) 545
EcoT14I CCWWGG 4 cut(s) 476, 930, 1262, 1380
EcoT22I ATGCAT 2 cut(s) 452, 950
ErhI CCWWGG 4 cut(s) 476, 930, 1262, 1380
Esp3I CGTCTC 2 cut(s) 144, 1187
FaeI CATG 9 cut(s) 386, 480, 551, 934, 979, 995, 1013, 1082, 1333
FalI AAGNNNNNCTT 2 cut(s) 1412, 1444
FaqI GGGAC 2 cut(s) 898, 1067
FatI CATG 9 cut(s) 382, 476, 547, 930, 975, 991, 1009, 1078, 1329
FauNDI CATATG 2 cut(s) 694, 1308
FbaI TGATCA 1 cut(s) 1135
FblI GTMKAC 1 cut(s) 1278
Fnu4HI GCNGC 5 cut(s) 6, 104, 123, 1316, 1459
FokI GGATG 4 cut(s) 71, 185, 415, 908
Fsp4HI GCNGC 5 cut(s) 6, 104, 123, 1316, 1459
FspBI CTAG 3 cut(s) 99, 290, 527
FspI TGCGCA 1 cut(s) 318
GlaI GCGC 3 cut(s) 54, 318, 381
GluI GCNGC 5 cut(s) 6, 104, 123, 1316, 1459
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 1125
HapII CCGG 3 cut(s) 72, 84, 921
HgaI GACGC 1 cut(s) 1169
HhaI GCGC 3 cut(s) 55, 319, 382
Hin1II CATG 9 cut(s) 386, 480, 551, 934, 979, 995, 1013, 1082, 1333
Hin6I GCGC 3 cut(s) 53, 317, 380
HinP1I GCGC 3 cut(s) 53, 317, 380
HincII GTYRAC 3 cut(s) 436, 700, 1000
HindII GTYRAC 3 cut(s) 436, 700, 1000
HindIII AAGCTT 2 cut(s) 561, 811
HinfI GANTC 9 cut(s) 80, 162, 195, 293, 789, 835, 1074, 1290, 1493
HpaII CCGG 3 cut(s) 72, 84, 921
HphI GGTGA 3 cut(s) 382, 910, 1344
Hpy166II GTNNAC 6 cut(s) 436, 700, 776, 1000, 1156, 1279
Hpy188I TCNGA 7 cut(s) 91, 112, 409, 796, 847, 1488, 1519
Hpy8I GTNNAC 6 cut(s) 436, 700, 776, 1000, 1156, 1279
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 2 cut(s) 70, 1538
HpyCH4III ACNGT 6 cut(s) 217, 443, 704, 773, 851, 1448
HpyCH4IV ACGT 1 cut(s) 1283
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 359
HpyF3I CTNAG 6 cut(s) 354, 363, 793, 1487, 1541, 1584
HpySE526I ACGT 1 cut(s) 1283
Hsp92II CATG 9 cut(s) 386, 480, 551, 934, 979, 995, 1013, 1082, 1333
HspAI GCGC 3 cut(s) 53, 317, 380
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 5 cut(s) 91, 136, 645, 1135, 1174
Lsp1109I GCAGC 4 cut(s) 90, 134, 1302, 1445
LweI GCATC 5 cut(s) 126, 304, 1236, 1302, 1394
MaeI CTAG 3 cut(s) 99, 290, 527
MaeII ACGT 1 cut(s) 1283
MaeIII GTNAC 3 cut(s) 370, 704, 916
MalI GATC 5 cut(s) 93, 138, 647, 1137, 1176
MboI GATC 5 cut(s) 91, 136, 645, 1135, 1174
MflI RGATCY 2 cut(s) 91, 1174
MluCI AATT 8 cut(s) 305, 410, 484, 533, 758, 1268, 1423, 1472
MlyI GAGTC 2 cut(s) 1284, 1502
MmeI TCCRAC 1 cut(s) 69
Mph1103I ATGCAT 2 cut(s) 452, 950
MroXI GAANNNNTTC 1 cut(s) 1424
MseI TTAA 6 cut(s) 66, 506, 728, 1206, 1392, 1443
MslI CAYNNNNRTG 1 cut(s) 965
MspI CCGG 3 cut(s) 72, 84, 921
MspR9I CCNGG 5 cut(s) 73, 85, 1129, 1455, 1502
Mva1269I GAATGC 1 cut(s) 450
MvaI CCWGG 3 cut(s) 1129, 1455, 1502
MwoI GCNNNNNNNGC 2 cut(s) 262, 359
NciI CCSGG 2 cut(s) 73, 85
NcoI CCATGG 2 cut(s) 476, 930
NdeI CATATG 2 cut(s) 694, 1308
NdeII GATC 5 cut(s) 91, 136, 645, 1135, 1174
NlaIII CATG 9 cut(s) 386, 480, 551, 934, 979, 995, 1013, 1082, 1333
NlaIV GGNNCC 3 cut(s) 179, 1126, 1521
NmuCI GTSAC 2 cut(s) 370, 916
NsbI TGCGCA 1 cut(s) 318
NsiI ATGCAT 2 cut(s) 452, 950
NspI RCATGY 2 cut(s) 386, 979
PaeI GCATGC 1 cut(s) 386
PciI ACATGT 1 cut(s) 975
PctI GAATGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 1424
PfeI GAWTC 7 cut(s) 80, 162, 195, 293, 789, 835, 1074
Pfl23II CGTACG 1 cut(s) 632
PfoI TCCNGGA 2 cut(s) 71, 1500
PkrI GCNGC 5 cut(s) 7, 105, 124, 1317, 1460
PleI GAGTC 2 cut(s) 1284, 1501
PpsI GAGTC 2 cut(s) 1284, 1501
Ppu21I YACGTR 1 cut(s) 1284
PscI ACATGT 1 cut(s) 975
PshAI GACNNNNGTC 1 cut(s) 441
PsiI TTATAA 1 cut(s) 687
Psp6I CCWGG 3 cut(s) 1127, 1453, 1500
PspEI GGTNACC 1 cut(s) 370
PspGI CCWGG 3 cut(s) 1127, 1453, 1500
PspLI CGTACG 1 cut(s) 632
PspN4I GGNNCC 3 cut(s) 179, 1126, 1521
PspPI GGNCC 3 cut(s) 480, 1124, 1519
PsuI RGATCY 2 cut(s) 91, 1174
RsaI GTAC 3 cut(s) 311, 634, 770
RsaNI GTAC 3 cut(s) 310, 633, 769
RseI CAYNNNNRTG 1 cut(s) 965
SaqAI TTAA 6 cut(s) 66, 506, 728, 1206, 1392, 1443
SatI GCNGC 5 cut(s) 6, 104, 123, 1316, 1459
Sau3AI GATC 5 cut(s) 91, 136, 645, 1135, 1174
Sau96I GGNCC 3 cut(s) 480, 1124, 1519
SchI GAGTC 2 cut(s) 1284, 1502
ScrFI CCNGG 5 cut(s) 73, 85, 1129, 1455, 1502
SfaNI GCATC 5 cut(s) 126, 304, 1236, 1302, 1394
SfcI CTRYAG 1 cut(s) 850
SinI GGWCC 2 cut(s) 480, 1519
SmiMI CAYNNNNRTG 1 cut(s) 965
SmlI CTYRAG 2 cut(s) 167, 395
SmoI CTYRAG 2 cut(s) 167, 395
SphI GCATGC 1 cut(s) 386
Sse9I AATT 8 cut(s) 305, 410, 484, 533, 758, 1268, 1423, 1472
SspMI CTAG 3 cut(s) 99, 290, 527
StyD4I CCNGG 5 cut(s) 71, 83, 1127, 1453, 1500
StyI CCWWGG 4 cut(s) 476, 930, 1262, 1380
TaaI ACNGT 6 cut(s) 217, 443, 704, 773, 851, 1448
TaiI ACGT 1 cut(s) 1286
TaqI TCGA 3 cut(s) 12, 41, 523
TasI AATT 8 cut(s) 305, 410, 484, 533, 758, 1268, 1423, 1472
TfiI GAWTC 7 cut(s) 80, 162, 195, 293, 789, 835, 1074
Tru1I TTAA 6 cut(s) 66, 506, 728, 1206, 1392, 1443
Tru9I TTAA 6 cut(s) 66, 506, 728, 1206, 1392, 1443
TseFI GTSAC 2 cut(s) 370, 916
TseI GCWGC 5 cut(s) 5, 103, 122, 1315, 1458
Tsp45I GTSAC 2 cut(s) 370, 916
TspDTI ATGAA 6 cut(s) 187, 509, 798, 848, 998, 1295
VpaK11BI GGWCC 2 cut(s) 480, 1519
XapI RAATTY 4 cut(s) 533, 758, 1268, 1423
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 2 cut(s) 386, 979
XmiI GTMKAC 1 cut(s) 1278
XmnI GAANNNNTTC 1 cut(s) 1424
XspI CTAG 3 cut(s) 99, 290, 527
Zsp2I ATGCAT 2 cut(s) 452, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.