RchiOBHm_Chr2g0127641

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006101
Physical Location & Seq
Forward (+)
42519344 .. 42524158
4815 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49949

Sequence Viewer

Length: 1617 bp
ATGGCTGCCGTCTTCCAACACTCGCCGGCCACCGCCACTCCTCTTCACGACCCTCTCTTGCCCTCCCTCTGGTTGATAAAGCAAGCGCTCGATGACTTCAACTCCGGAAACCATTCCGGGTCGGATCTCACTAAGCTTCTATCCGATTGCATCGCAGCTTTCAAGGACAGCGTTCAGTACCGAGACGACACCAGATTCCTCAAGATTTGGCTCCTCTATATGGGGTTTATTGAGGATTTCGAAAGCTTGTTTATGGAAATGTTGGATAGAAAGATATGTATTGGACACTCTTTGCTTTATGTTTGGTATGCCTCCTTTCTAGAGTCCAAGGGCAAGTTATACGATGCGCACATGGTTTATCAGATGGGTTTTTCAAGGAATGCTAAGCCTGCTGAGTGGGTGAAGAAAGCACACGGCTTATTTCTTGAGAGGATGTCTGAATTAGTTTCTTCAACTCACAAGGTTGACGATGGTGAATCCATTCAGTTTGACAGCCACATCAATCCATGGTCCAGTTCCACAATGAAAGCCCTATTACAGAAAATTACTCCTCAACTTATGAAATACGATGGATATCACTCCACTAATAAAGCTTACCCAGGAAAAGTGGCATTGTCTTCTTTGAAGAACTCATCAAGGAACAAGATTATTGAGATAGGTCGTACAAAATACCAGATTACGGGTTACGCAGGTCAAGGTGGTTTTGCTCAAGTATATAAAGCATATGTCAACTGTAACCCTGATGATGTTGTTGCATTAAAGATACAAAAGCCTGCTTTCCCGTGGGAATTCTATATGTACCGTGAACTTGATCAACGAATCTCAGACAAGGAAAGGTCAAGCTTTGGTTTCGCTCAAAGAATGCATCTCTATTCTGACTGTAGTATACTTGTCTGCAACTATATTGCTAATGGGACACTTCAGGATGTAATAAACTCATTTGCAGTCACCGGAAAATCCATGGAAGAAGTGTTATGCATTTATTACACCATAGAAATGCTCTACATGTTAGAAACTCTGCATGACGTTGGCATCATTCATGGAGATTTCAAGCCTGATAATCTGCTTATTCGCTATTCTCGGGACTTCCCCACAAAAAATGGATTTCATGACAGAAATGAGCTTACAAAAGATGGATTTCGGGACAGAAGTGGCCCTTGGCTTGATCAGGGTCTTTGCCTTGTGGACTGGGGAAGAGGGATAGATCTGCGTCTCTTTCCTGACAATATGGAGTTTAAGGGAGATTGCCGAACTTCTGGATTTCGTTGTGTGGAGATGCAAGAGAATAAGCCTTGGACATTTCAGGTAGACACATATGGACTCTGTGTTGTTGTCCATATGATGCTGCATAATTCTTACATGGAGATTGACAAGAAACCATCACCTGATGGTGGTTACGCTTATCTACCCAAGGCATCTTTTAAAAGATACTGGAAGGTTGAGCTCTGGAAGAATTTCTTTGTGAAACTGCTTAACAGTAATCCTGGCGGCAATGACAAGAAATTGTTGCAAAATCTGAGGGAGTCCTTTCAGGAATACATGTGCTCGGACCCTCACCTTATAAAGAAACTAAGCGATTTACTGGCAAAGCAAAGGAAATCAATGTGTTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

538

Amino Acids

62.03

Weight (kDa)

7.87

Isoelectric Point (pI)

32.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 15 - 123 1.2e-23 Mad3/BUB1 homology region 1
Pkinase PF00069 225 - 451 7.3e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1562
Acc16I TGCGCA 1 cut(s) 348
Acc36I ACCTGC 1 cut(s) 680
AccI GTMKAC 2 cut(s) 886, 1308
AccIII TCCGGA 1 cut(s) 104
AciI CCGC 2 cut(s) 33, 1488
AclWI GGATC 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 2 cut(s) 788, 1453
AcuI CTGAAG 1 cut(s) 905
AfaI GTAC 3 cut(s) 179, 664, 800
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1391
AflIII ACRYGT 2 cut(s) 1005, 1539
AgsI TTSAA 6 cut(s) 100, 163, 375, 453, 625, 1051
AjnI CCWGG 2 cut(s) 598, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 1141, 1173
AluBI AGCT 7 cut(s) 136, 158, 246, 593, 843, 1123, 1444
AluI AGCT 7 cut(s) 136, 158, 246, 593, 843, 1123, 1444
Alw21I GWGCWC 2 cut(s) 1446, 1547
Alw26I GTCTC 2 cut(s) 177, 1217
AlwI GGATC 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 1080
Aor13HI TCCGGA 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 2 cut(s) 27, 1153
ApeKI GCWGC 3 cut(s) 5, 155, 1345
ApoI RAATTY 2 cut(s) 788, 1453
Asp700I GAANNNNTTC 3 cut(s) 112, 480, 1454
AspLEI GCGC 2 cut(s) 88, 349
AspS9I GGNCC 3 cut(s) 510, 1154, 1549
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 5 cut(s) 412, 485, 940, 1374, 1547
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 1080
AvaII GGWCC 2 cut(s) 510, 1549
BanII GRGCYC 1 cut(s) 1446
BbsI GAAGAC 2 cut(s) 4, 609
Bbv12I GWGCWC 2 cut(s) 1446, 1547
BbvI GCAGC 2 cut(s) 167, 1332
BccI CCATC 6 cut(s) 358, 464, 563, 1127, 1382, 1387
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 2 cut(s) 600, 1485
BclI TGATCA 2 cut(s) 811, 1165
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 2 cut(s) 177, 1217
BfaI CTAG 1 cut(s) 320
BfmI CTRYAG 1 cut(s) 880
BfoI RGCGCY 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 680
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 4 cut(s) 6, 156, 1346, 1489
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 4 cut(s) 7, 157, 1347, 1490
Bme1390I CCNGG 3 cut(s) 118, 600, 1485
Bme18I GGWCC 2 cut(s) 510, 1549
BmeT110I CYCGRG 1 cut(s) 1080
BmgT120I GGNCC 3 cut(s) 510, 1154, 1549
BmiI GGNNCC 2 cut(s) 212, 1551
BmrFI CCNGG 3 cut(s) 118, 600, 1485
BmrI ACTGGG 1 cut(s) 1198
BmsI GCATC 7 cut(s) 159, 334, 874, 1041, 1266, 1332, 1424
BmuI ACTGGG 1 cut(s) 1198
BpiI GAAGAC 2 cut(s) 4, 609
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 185, 446, 693
BpuMI CCSGG 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 8 cut(s) 327, 506, 598, 782, 960, 1157, 1292, 1410
BsaWI WCCGGW 2 cut(s) 104, 950
BsaXI ACNNNNNCTCC 4 cut(s) 22, 52, 86, 116
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1391
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 4 cut(s) 513, 1193, 1436, 1587
Bse3DI GCAATG 1 cut(s) 1498
Bse8I GATNNNNATC 1 cut(s) 573
BseAI TCCGGA 1 cut(s) 104
BseBI CCWGG 2 cut(s) 600, 1485
BseDI CCNNGG 8 cut(s) 327, 506, 598, 782, 960, 1157, 1292, 1410
BseGI GGATG 2 cut(s) 438, 931
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1391
BseMI GCAATG 1 cut(s) 1498
BseMII CTCAG 3 cut(s) 384, 837, 1508
BseNI ACTGG 4 cut(s) 513, 1193, 1436, 1587
BseRI GAGGAG 3 cut(s) 30, 203, 540
BseXI GCAGC 2 cut(s) 167, 1332
BshFI GGCC 2 cut(s) 29, 1155
BsiHKAI GWGCWC 2 cut(s) 1446, 1547
BsiHKCI CYCGRG 1 cut(s) 1080
BsiSI CCGG 4 cut(s) 26, 105, 117, 951
BslFI GGGAC 3 cut(s) 928, 1097, 1157
BslI CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1391
BsmAI GTCTC 2 cut(s) 177, 1217
BsmBI CGTCTC 2 cut(s) 177, 1217
BsmFI GGGAC 3 cut(s) 928, 1097, 1157
BsmI GAATGC 2 cut(s) 385, 867
BsnI GGCC 2 cut(s) 29, 1155
BsoBI CYCGRG 1 cut(s) 1080
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 2 cut(s) 1446, 1547
Bsp13I TCCGGA 1 cut(s) 104
Bsp143I GATC 4 cut(s) 124, 811, 1165, 1204
Bsp1720I GCTNAGC 1 cut(s) 384
Bsp19I CCATGG 2 cut(s) 506, 960
BspACI CCGC 2 cut(s) 33, 1488
BspANI GGCC 2 cut(s) 29, 1155
BspCNI CTCAG 3 cut(s) 385, 836, 1509
BspEI TCCGGA 1 cut(s) 104
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 2 cut(s) 212, 1551
BspMI ACCTGC 1 cut(s) 680
BspPI GGATC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 1498
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 4 cut(s) 513, 1193, 1436, 1587
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 8 cut(s) 327, 506, 598, 782, 960, 1157, 1292, 1410
BssMI GATC 4 cut(s) 124, 811, 1165, 1204
BssNAI GTATAC 1 cut(s) 887
BssT1I CCWWGG 6 cut(s) 327, 506, 960, 1157, 1292, 1410
Bst1107I GTATAC 1 cut(s) 887
Bst2UI CCWGG 2 cut(s) 600, 1485
Bst4CI ACNGT 4 cut(s) 734, 803, 881, 1478
Bst6I CTCTTC 2 cut(s) 48, 1189
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 4 cut(s) 27, 84, 390, 774
BstDEI CTNAG 7 cut(s) 132, 384, 393, 823, 1517, 1571, 1614
BstDSI CCRYGG 3 cut(s) 506, 782, 960
BstF5I GGATG 2 cut(s) 438, 931
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 2 cut(s) 88, 349
BstKTI GATC 4 cut(s) 127, 814, 1168, 1207
BstMAI GTCTC 2 cut(s) 177, 1217
BstMBI GATC 4 cut(s) 124, 811, 1165, 1204
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 2 cut(s) 600, 1485
BstNSI RCATGY 2 cut(s) 1009, 1543
BstSCI CCNGG 3 cut(s) 116, 598, 1483
BstSFI CTRYAG 1 cut(s) 880
BstV1I GCAGC 2 cut(s) 167, 1332
BstV2I GAAGAC 2 cut(s) 4, 609
BstX2I RGATCY 2 cut(s) 124, 1204
BstYI RGATCY 2 cut(s) 124, 1204
BstZ17I GTATAC 1 cut(s) 887
BsuRI GGCC 2 cut(s) 29, 1155
BtgI CCRYGG 3 cut(s) 506, 782, 960
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 2 cut(s) 438, 931
BveI ACCTGC 1 cut(s) 680
Cac8I GCNNGC 4 cut(s) 27, 84, 390, 774
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 2 cut(s) 88, 349
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 510, 1154, 1549
CseI GACGC 1 cut(s) 1199
Csp6I GTAC 3 cut(s) 178, 663, 799
CviAII CATG 9 cut(s) 352, 507, 961, 1006, 1022, 1040, 1109, 1360, 1540
CviQI GTAC 3 cut(s) 178, 663, 799
DdeI CTNAG 7 cut(s) 132, 384, 393, 823, 1517, 1571, 1614
DpnI GATC 4 cut(s) 126, 813, 1167, 1206
DpnII GATC 4 cut(s) 124, 811, 1165, 1204
DraI TTTAAA 1 cut(s) 1423
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 48, 1189
EarI CTCTTC 2 cut(s) 48, 1189
Ecl136II GAGCTC 1 cut(s) 1444
Eco130I CCWWGG 6 cut(s) 327, 506, 960, 1157, 1292, 1410
Eco24I GRGCYC 1 cut(s) 1446
Eco32I GATATC 1 cut(s) 575
Eco47I GGWCC 2 cut(s) 510, 1549
Eco47III AGCGCT 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 1080
EcoICRI GAGCTC 1 cut(s) 1444
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 598, 1483
EcoRV GATATC 1 cut(s) 575
EcoT14I CCWWGG 6 cut(s) 327, 506, 960, 1157, 1292, 1410
EcoT22I ATGCAT 2 cut(s) 867, 980
EcoT38I GRGCYC 1 cut(s) 1446
ErhI CCWWGG 6 cut(s) 327, 506, 960, 1157, 1292, 1410
Esp3I CGTCTC 2 cut(s) 177, 1217
FaeI CATG 9 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112, 1363, 1543
FalI AAGNNNNNCTT 4 cut(s) 1141, 1173, 1442, 1474
FaqI GGGAC 3 cut(s) 928, 1097, 1157
FatI CATG 9 cut(s) 351, 506, 960, 1005, 1021, 1039, 1108, 1359, 1539
FauNDI CATATG 3 cut(s) 724, 1315, 1338
FbaI TGATCA 2 cut(s) 811, 1165
FblI GTMKAC 2 cut(s) 886, 1308
Fnu4HI GCNGC 4 cut(s) 6, 156, 1346, 1489
FokI GGATG 2 cut(s) 445, 938
FriOI GRGCYC 1 cut(s) 1446
Fsp4HI GCNGC 4 cut(s) 6, 156, 1346, 1489
FspAI RTGCGCAY 1 cut(s) 348
FspBI CTAG 1 cut(s) 320
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 2 cut(s) 87, 348
GluI GCNGC 4 cut(s) 6, 156, 1346, 1489
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 2 cut(s) 29, 1155
HapII CCGG 4 cut(s) 26, 105, 117, 951
HgaI GACGC 1 cut(s) 1199
HhaI GCGC 2 cut(s) 88, 349
Hin1II CATG 9 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112, 1363, 1543
Hin6I GCGC 2 cut(s) 86, 347
HinP1I GCGC 2 cut(s) 86, 347
HincII GTYRAC 2 cut(s) 466, 730
HindII GTYRAC 2 cut(s) 466, 730
HindIII AAGCTT 4 cut(s) 134, 244, 591, 841
HinfI GANTC 6 cut(s) 195, 323, 476, 819, 1320, 1523
HpaII CCGG 4 cut(s) 26, 105, 117, 951
HphI GGTGA 5 cut(s) 412, 485, 940, 1374, 1547
Hpy166II GTNNAC 6 cut(s) 466, 730, 806, 887, 1186, 1309
Hpy188I TCNGA 8 cut(s) 124, 145, 363, 439, 826, 877, 1518, 1549
Hpy8I GTNNAC 6 cut(s) 466, 730, 806, 887, 1186, 1309
HpyAV CCTTC 1 cut(s) 1429
HpyCH4III ACNGT 4 cut(s) 734, 803, 881, 1478
HpyCH4IV ACGT 1 cut(s) 1026
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 7 cut(s) 132, 384, 393, 823, 1517, 1571, 1614
HpySE526I ACGT 1 cut(s) 1026
Hsp92II CATG 9 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112, 1363, 1543
HspAI GCGC 2 cut(s) 86, 347
Kpn2I TCCGGA 1 cut(s) 104
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 811, 1165
Kzo9I GATC 4 cut(s) 124, 811, 1165, 1204
LmnI GCTCC 1 cut(s) 216
Lsp1109I GCAGC 2 cut(s) 167, 1332
LweI GCATC 7 cut(s) 159, 334, 874, 1041, 1266, 1332, 1424
MaeI CTAG 1 cut(s) 320
MaeII ACGT 1 cut(s) 1026
MaeIII GTNAC 4 cut(s) 683, 734, 946, 1394
MalI GATC 4 cut(s) 126, 813, 1167, 1206
MboI GATC 4 cut(s) 124, 811, 1165, 1204
MboII GAAGA 9 cut(s) 4, 35, 415, 441, 609, 637, 977, 1206, 1462
MflI RGATCY 2 cut(s) 124, 1204
MhlI GDGCHC 2 cut(s) 1446, 1547
MluCI AATT 6 cut(s) 440, 543, 788, 1351, 1453, 1502
MlyI GAGTC 3 cut(s) 332, 1314, 1532
MmeI TCCRAC 3 cut(s) 40, 102, 243
Mph1103I ATGCAT 2 cut(s) 867, 980
MroI TCCGGA 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 3 cut(s) 112, 480, 1454
MseI TTAA 4 cut(s) 758, 1236, 1422, 1473
MslI CAYNNNNRTG 1 cut(s) 995
MspI CCGG 4 cut(s) 26, 105, 117, 951
MspR9I CCNGG 3 cut(s) 118, 600, 1485
Mva1269I GAATGC 2 cut(s) 385, 867
MvaI CCWGG 2 cut(s) 600, 1485
MwoI GCNNNNNNNGC 1 cut(s) 389
NaeI GCCGGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 2 cut(s) 506, 960
NdeI CATATG 3 cut(s) 724, 1315, 1338
NdeII GATC 4 cut(s) 124, 811, 1165, 1204
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 9 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112, 1363, 1543
NlaIV GGNNCC 2 cut(s) 212, 1551
NmuCI GTSAC 1 cut(s) 946
NsbI TGCGCA 1 cut(s) 348
NsiI ATGCAT 2 cut(s) 867, 980
NspI RCATGY 2 cut(s) 1009, 1543
NspV TTCGAA 1 cut(s) 240
PagI TCATGA 1 cut(s) 1108
PciI ACATGT 2 cut(s) 1005, 1539
PctI GAATGC 2 cut(s) 385, 867
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 3 cut(s) 112, 480, 1454
PfeI GAWTC 3 cut(s) 195, 476, 819
PkrI GCNGC 4 cut(s) 7, 157, 1347, 1490
PleI GAGTC 3 cut(s) 331, 1314, 1531
PpsI GAGTC 3 cut(s) 331, 1314, 1531
PscI ACATGT 2 cut(s) 1005, 1539
PsiI TTATAA 1 cut(s) 1562
Psp124BI GAGCTC 1 cut(s) 1446
Psp6I CCWGG 2 cut(s) 598, 1483
PspGI CCWGG 2 cut(s) 598, 1483
PspN4I GGNNCC 2 cut(s) 212, 1551
PspPI GGNCC 3 cut(s) 510, 1154, 1549
PsuI RGATCY 2 cut(s) 124, 1204
RsaI GTAC 3 cut(s) 179, 664, 800
RsaNI GTAC 3 cut(s) 178, 663, 799
RseI CAYNNNNRTG 1 cut(s) 995
SacI GAGCTC 1 cut(s) 1446
SaqAI TTAA 4 cut(s) 758, 1236, 1422, 1473
SatI GCNGC 4 cut(s) 6, 156, 1346, 1489
Sau3AI GATC 4 cut(s) 124, 811, 1165, 1204
Sau96I GGNCC 3 cut(s) 510, 1154, 1549
SchI GAGTC 3 cut(s) 332, 1314, 1532
ScrFI CCNGG 3 cut(s) 118, 600, 1485
SduI GDGCHC 2 cut(s) 1446, 1547
SfaNI GCATC 7 cut(s) 159, 334, 874, 1041, 1266, 1332, 1424
SfcI CTRYAG 1 cut(s) 880
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 2 cut(s) 510, 1549
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 3 cut(s) 200, 425, 708
SmoI CTYRAG 3 cut(s) 200, 425, 708
Sse9I AATT 6 cut(s) 440, 543, 788, 1351, 1453, 1502
SsiI CCGC 2 cut(s) 33, 1488
SspMI CTAG 1 cut(s) 320
SstI GAGCTC 1 cut(s) 1446
StyD4I CCNGG 3 cut(s) 116, 598, 1483
StyI CCWWGG 6 cut(s) 327, 506, 960, 1157, 1292, 1410
TaaI ACNGT 4 cut(s) 734, 803, 881, 1478
TaiI ACGT 1 cut(s) 1029
TaqI TCGA 2 cut(s) 90, 240
TasI AATT 6 cut(s) 440, 543, 788, 1351, 1453, 1502
TauI GCSGC 1 cut(s) 1491
TfiI GAWTC 3 cut(s) 195, 476, 819
Tru1I TTAA 4 cut(s) 758, 1236, 1422, 1473
Tru9I TTAA 4 cut(s) 758, 1236, 1422, 1473
TseFI GTSAC 1 cut(s) 946
TseI GCWGC 3 cut(s) 5, 155, 1345
Tsp45I GTSAC 1 cut(s) 946
TspDTI ATGAA 4 cut(s) 539, 575, 1028, 1097
VpaK11BI GGWCC 2 cut(s) 510, 1549
XapI RAATTY 2 cut(s) 788, 1453
XbaI TCTAGA 1 cut(s) 319
XceI RCATGY 2 cut(s) 1009, 1543
XmiI GTMKAC 2 cut(s) 886, 1308
XmnI GAANNNNTTC 3 cut(s) 112, 480, 1454
XspI CTAG 1 cut(s) 320
Zsp2I ATGCAT 2 cut(s) 867, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.