Prupe.3G139600_v2.0.a1

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0006101
Physical Location & Seq
Reverse (-)
14727730 .. 14729655
1926 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G139600.1

Sequence Viewer

Length: 939 bp
GTCAATGTATTCCTGCTGATTGTCCAAACCGTGGTACTCACAGGTTGTACGAAGTACCAGATCATAGGTTGCGCAGGTCAGGGTGGTTCTGCTCAAGTGTATAAAGCATATGTCAGCTGTAATCCTGACGATGTTGTTGCATTGAAGATACAAAAGCCTGCTTTCCCTTGGGAGTTCTATATGTATCGTCAACTGGATCAACGGATCTCAGACAAGGAAGTCAAGCTTTGGTCTTGCTATCTTTATTCTGACTGTAGCATACTTGTCTGTGATTATCTTGCTAATGGAACACTTCAGGATGCAAAAAACTCATTTGCAGTCATTGGCAAGTCCATGGAAGAAGTGTTATGCATTTATTACACCATAGAAATGCTCTACATGCTAGAAACTCTGCTTGGTGTTGGCATCGTTCGTGGAGATTTCAAGCCTGATAATTTGCTTATTCGTTATGCTAGGAATGACTTTACAACAGATGGATTTTCTGATAGAAGTGGTCCCTGGCATGATCAGGGTCTTTGCCTTGTCGACTGGGGAAGAGGGATAGACATGCATCTCTTTCCTGACAATATGGAGTTTAAGGGAGATTGCCGGACTTCTGGATTTCGTTGTGCGGAGATGCAAGAGAATAGGCCATGGACATTTCAGGTAGACACATATGGCCTCTGTGTTGTTGTCCATTTGATGCTGCATAATTCTTACATGGAAATTGAAAAGAAACTATCACCTGAAGGTGGTTACATTTATCAACCCAAATATTGGAATGCTGAACTCTGGAGGAATTTATTTACAAAACGGCTTAACAGTGGCCCCCGCGCTAATGACAAGAAGTTGCTGCAGGATTTGAGGGAGTCCTTCCAGGATTACATGTCCTCGGACCCTCTGCTTATAAAGAAACTTAGTGGTTTACTGGTTAAGCAAAGGGCCACATTGGGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

313

Amino Acids

35.8

Weight (kDa)

6.15

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
AasI GACNNNNNNGTC 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 65
AccB7I CCANNNNNTGG 2 cut(s) 31, 756
AccI GTMKAC 2 cut(s) 525, 648
AccII CGCG 1 cut(s) 813
AciI CCGC 2 cut(s) 611, 811
AclWI GGATC 2 cut(s) 204, 212
AcsI RAATTY 1 cut(s) 778
AcuI CTGAAG 2 cut(s) 278, 747
AfaI GTAC 3 cut(s) 36, 49, 56
AfiI CCNNNNNNNGG 3 cut(s) 31, 731, 756
AflIII ACRYGT 1 cut(s) 864
AgsI TTSAA 3 cut(s) 145, 424, 710
AjnI CCWGG 2 cut(s) 497, 855
AjuI GAANNNNNNNTTGG 2 cut(s) 378, 410
AluBI AGCT 2 cut(s) 117, 226
AluI AGCT 2 cut(s) 117, 226
AlwI GGATC 2 cut(s) 204, 212
AoxI GGCC 4 cut(s) 629, 658, 805, 921
ApeKI GCWGC 2 cut(s) 685, 832
ApoI RAATTY 1 cut(s) 778
AspLEI GCGC 2 cut(s) 74, 815
AspS9I GGNCC 4 cut(s) 494, 806, 874, 921
AsuHPI GGTGA 1 cut(s) 714
AvaII GGWCC 2 cut(s) 494, 874
BbvI GCAGC 2 cut(s) 672, 819
BccI CCATC 1 cut(s) 467
BceAI ACGGC 1 cut(s) 809
BcgI CGANNNNNNTGC 2 cut(s) 119, 153
BciT130I CCWGG 2 cut(s) 499, 857
BclI TGATCA 1 cut(s) 505
BfaI CTAG 2 cut(s) 383, 453
BfmI CTRYAG 2 cut(s) 253, 833
BfuAI ACCTGC 1 cut(s) 65
BisI GCNGC 2 cut(s) 686, 833
BlsI GCNGC 2 cut(s) 687, 834
Bme1390I CCNGG 2 cut(s) 499, 857
Bme18I GGWCC 2 cut(s) 494, 874
BmgT120I GGNCC 4 cut(s) 494, 806, 874, 921
BmiI GGNNCC 3 cut(s) 496, 808, 876
BmrFI CCNGG 2 cut(s) 499, 857
BmrI ACTGGG 1 cut(s) 538
BmsI GCATC 5 cut(s) 289, 414, 559, 606, 672
BmuI ACTGGG 1 cut(s) 538
BpmI CTGGAG 1 cut(s) 793
BpuEI CTTGAG 1 cut(s) 78
BsaJI CCNNGG 6 cut(s) 30, 167, 333, 497, 632, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 731, 756
Bse1I ACTGG 3 cut(s) 198, 533, 912
BseBI CCWGG 2 cut(s) 499, 857
BseDI CCNNGG 6 cut(s) 30, 167, 333, 497, 632, 870
BseGI GGATG 1 cut(s) 304
BseLI CCNNNNNNNGG 3 cut(s) 31, 731, 756
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 3 cut(s) 198, 533, 912
BseXI GCAGC 2 cut(s) 672, 819
Bsh1236I CGCG 1 cut(s) 813
BshFI GGCC 4 cut(s) 631, 660, 807, 923
BsiSI CCGG 1 cut(s) 589
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 3 cut(s) 31, 731, 756
BsmFI GGGAC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 4 cut(s) 631, 660, 807, 923
Bsp143I GATC 4 cut(s) 60, 196, 204, 505
Bsp19I CCATGG 2 cut(s) 333, 632
BspACI CCGC 2 cut(s) 611, 811
BspANI GGCC 4 cut(s) 631, 660, 807, 923
BspCNI CTCAG 1 cut(s) 221
BspFNI CGCG 1 cut(s) 813
BspLI GGNNCC 3 cut(s) 496, 808, 876
BspMAI CTGCAG 1 cut(s) 837
BspMI ACCTGC 1 cut(s) 65
BspPI GGATC 2 cut(s) 204, 212
BsrI ACTGG 3 cut(s) 198, 533, 912
BssECI CCNNGG 6 cut(s) 30, 167, 333, 497, 632, 870
BssMI GATC 4 cut(s) 60, 196, 204, 505
BssT1I CCWWGG 3 cut(s) 167, 333, 632
Bst2UI CCWGG 2 cut(s) 499, 857
Bst4CI ACNGT 3 cut(s) 31, 254, 803
Bst6I CTCTTC 1 cut(s) 529
BstC8I GCNNGC 1 cut(s) 159
BstDEI CTNAG 3 cut(s) 208, 896, 936
BstDSI CCRYGG 3 cut(s) 30, 333, 632
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 1 cut(s) 813
BstHHI GCGC 2 cut(s) 74, 815
BstKTI GATC 4 cut(s) 63, 199, 207, 508
BstMBI GATC 4 cut(s) 60, 196, 204, 505
BstMWI GCNNNNNNNGC 1 cut(s) 379
BstNI CCWGG 2 cut(s) 499, 857
BstNSI RCATGY 3 cut(s) 382, 550, 868
BstSCI CCNGG 2 cut(s) 497, 855
BstSFI CTRYAG 2 cut(s) 253, 833
BstUI CGCG 1 cut(s) 813
BstV1I GCAGC 2 cut(s) 672, 819
BstX2I RGATCY 1 cut(s) 204
BstYI RGATCY 1 cut(s) 204
BsuRI GGCC 4 cut(s) 631, 660, 807, 923
BtgI CCRYGG 3 cut(s) 30, 333, 632
BtsCI GGATG 1 cut(s) 304
BtsIMutI CAGTG 1 cut(s) 808
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 159
CfoI GCGC 2 cut(s) 74, 815
Cfr13I GGNCC 4 cut(s) 494, 806, 874, 921
Csp6I GTAC 3 cut(s) 35, 48, 55
CviAII CATG 7 cut(s) 334, 379, 503, 547, 633, 700, 865
CviJI RGCY 9 cut(s) 117, 157, 226, 427, 631, 660, 796, 807, 923
CviKI_1 RGCY 9 cut(s) 117, 157, 226, 427, 631, 660, 796, 807, 923
CviQI GTAC 3 cut(s) 35, 48, 55
DdeI CTNAG 3 cut(s) 208, 896, 936
DpnI GATC 4 cut(s) 62, 198, 206, 507
DpnII GATC 4 cut(s) 60, 196, 204, 505
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 529
Eco130I CCWWGG 3 cut(s) 167, 333, 632
Eco47I GGWCC 2 cut(s) 494, 874
Eco57I CTGAAG 2 cut(s) 278, 747
EcoRII CCWGG 2 cut(s) 497, 855
EcoT14I CCWWGG 3 cut(s) 167, 333, 632
EcoT22I ATGCAT 2 cut(s) 353, 552
ErhI CCWWGG 3 cut(s) 167, 333, 632
FaeI CATG 7 cut(s) 337, 382, 506, 550, 636, 703, 868
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 480
FatI CATG 7 cut(s) 333, 378, 502, 546, 632, 699, 864
FauI CCCGC 1 cut(s) 818
FauNDI CATATG 2 cut(s) 109, 655
FbaI TGATCA 1 cut(s) 505
FblI GTMKAC 2 cut(s) 525, 648
Fnu4HI GCNGC 2 cut(s) 686, 833
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 686, 833
FspBI CTAG 2 cut(s) 383, 453
FspI TGCGCA 1 cut(s) 73
GlaI GCGC 2 cut(s) 73, 814
GluI GCNGC 2 cut(s) 686, 833
GsuI CTGGAG 1 cut(s) 793
HaeIII GGCC 4 cut(s) 631, 660, 807, 923
HapII CCGG 1 cut(s) 589
HhaI GCGC 2 cut(s) 74, 815
Hin1II CATG 7 cut(s) 337, 382, 506, 550, 636, 703, 868
Hin6I GCGC 2 cut(s) 72, 813
HinP1I GCGC 2 cut(s) 72, 813
HincII GTYRAC 2 cut(s) 191, 526
HindII GTYRAC 2 cut(s) 191, 526
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 1 cut(s) 848
HpaII CCGG 1 cut(s) 589
HphI GGTGA 1 cut(s) 714
Hpy166II GTNNAC 4 cut(s) 191, 526, 649, 905
Hpy188I TCNGA 4 cut(s) 211, 250, 484, 874
Hpy188III TCNNGA 5 cut(s) 125, 296, 560, 597, 772
Hpy8I GTNNAC 4 cut(s) 191, 526, 649, 905
HpyAV CCTTC 2 cut(s) 722, 862
HpyCH4III ACNGT 3 cut(s) 31, 254, 803
HpyCH4V TGCA 8 cut(s) 140, 302, 317, 351, 550, 619, 688, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 379
HpyF3I CTNAG 3 cut(s) 208, 896, 936
Hsp92II CATG 7 cut(s) 337, 382, 506, 550, 636, 703, 868
HspAI GCGC 2 cut(s) 72, 813
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 4 cut(s) 60, 196, 204, 505
Lsp1109I GCAGC 2 cut(s) 672, 819
LweI GCATC 5 cut(s) 289, 414, 559, 606, 672
MaeI CTAG 2 cut(s) 383, 453
MaeIII GTNAC 1 cut(s) 734
MalI GATC 4 cut(s) 62, 198, 206, 507
MboI GATC 4 cut(s) 60, 196, 204, 505
MboII GAAGA 3 cut(s) 157, 350, 546
MflI RGATCY 1 cut(s) 204
MluCI AATT 4 cut(s) 433, 691, 705, 778
MlyI GAGTC 1 cut(s) 857
MnlI CCTC 6 cut(s) 530, 671, 768, 837, 880, 888
Mph1103I ATGCAT 2 cut(s) 353, 552
MseI TTAA 3 cut(s) 576, 798, 912
MslI CAYNNNNRTG 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 589
MspR9I CCNGG 2 cut(s) 499, 857
Mva1269I GAATGC 1 cut(s) 766
MvaI CCWGG 2 cut(s) 499, 857
MvnI CGCG 1 cut(s) 813
MwoI GCNNNNNNNGC 1 cut(s) 379
NcoI CCATGG 2 cut(s) 333, 632
NdeI CATATG 2 cut(s) 109, 655
NdeII GATC 4 cut(s) 60, 196, 204, 505
NlaIII CATG 7 cut(s) 337, 382, 506, 550, 636, 703, 868
NlaIV GGNNCC 3 cut(s) 496, 808, 876
NsbI TGCGCA 1 cut(s) 73
NsiI ATGCAT 2 cut(s) 353, 552
NspI RCATGY 3 cut(s) 382, 550, 868
PciI ACATGT 1 cut(s) 864
PctI GAATGC 1 cut(s) 766
PflMI CCANNNNNTGG 2 cut(s) 31, 756
PfoI TCCNGGA 1 cut(s) 855
PkrI GCNGC 2 cut(s) 687, 834
PleI GAGTC 1 cut(s) 856
PpsI GAGTC 1 cut(s) 856
PscI ACATGT 1 cut(s) 864
PsiI TTATAA 1 cut(s) 887
Psp6I CCWGG 2 cut(s) 497, 855
PspGI CCWGG 2 cut(s) 497, 855
PspN4I GGNNCC 3 cut(s) 496, 808, 876
PspPI GGNCC 4 cut(s) 494, 806, 874, 921
PstI CTGCAG 1 cut(s) 837
PsuI RGATCY 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 3 cut(s) 36, 49, 56
RsaNI GTAC 3 cut(s) 35, 48, 55
RseI CAYNNNNRTG 1 cut(s) 368
SalI GTCGAC 1 cut(s) 524
SaqAI TTAA 3 cut(s) 576, 798, 912
SatI GCNGC 2 cut(s) 686, 833
Sau3AI GATC 4 cut(s) 60, 196, 204, 505
Sau96I GGNCC 4 cut(s) 494, 806, 874, 921
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 2 cut(s) 499, 857
SetI ASST 8 cut(s) 46, 70, 79, 119, 228, 648, 727, 733
SfaNI GCATC 5 cut(s) 289, 414, 559, 606, 672
SfcI CTRYAG 2 cut(s) 253, 833
SinI GGWCC 2 cut(s) 494, 874
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 93
SmoI CTYRAG 1 cut(s) 93
Sse9I AATT 4 cut(s) 433, 691, 705, 778
SsiI CCGC 2 cut(s) 611, 811
SspI AATATT 1 cut(s) 755
SspMI CTAG 2 cut(s) 383, 453
StyD4I CCNGG 2 cut(s) 497, 855
StyI CCWWGG 3 cut(s) 167, 333, 632
TaaI ACNGT 3 cut(s) 31, 254, 803
TaqI TCGA 1 cut(s) 525
TasI AATT 4 cut(s) 433, 691, 705, 778
Tru1I TTAA 3 cut(s) 576, 798, 912
Tru9I TTAA 3 cut(s) 576, 798, 912
TscAI CASTG 1 cut(s) 808
TseI GCWGC 2 cut(s) 685, 832
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 1 cut(s) 808
Van91I CCANNNNNTGG 2 cut(s) 31, 756
VpaK11BI GGWCC 2 cut(s) 494, 874
XapI RAATTY 1 cut(s) 778
XceI RCATGY 3 cut(s) 382, 550, 868
XmiI GTMKAC 2 cut(s) 525, 648
XspI CTAG 2 cut(s) 383, 453
Zsp2I ATGCAT 2 cut(s) 353, 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.