Rh2BG333300

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0006101
Physical Location & Seq
Reverse (-)
44054340 .. 44059015
4676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG333300.1

Sequence Viewer

Length: 1185 bp
ATGTCTGAATTAGTTTCTTCAACTCACAAGGTTGACGATGGTGAATCCATTCAGTTTGACAGCCACATCAATCCATGGTCCAGTTCCACAATGAAAGCCCTATTACAGAAAATTACTCCTCAACTTATGAAATACGATGGATATCACTCCACTAATAAAGCTTACCCAGGAAAAGTGGCATTGTCTTCTTTGAAGAACTCATCAAGGAACAAGATTATTGAGATAGGTCGTACAAAATACCAGATTACGGGTTACGCAGGTCAAGGTGGTTTTGCTCAAGTATATAAAGCATATGTCAACTGTAACCCTGATGATGTTGTTGCATTAAAGATACAAAAGCCTGCTTTCCCGTGGGAATTCTATATGTACCGTGAACTTGATCAACGAATCTCAGACAAGGAAAGGTCAAGCTTTGGTTTCGCTCAAAGAATGCATCTCTATTCTGACTGTAGTATACTTGTCTGCAACTATATTGCTAATGGGACACTTCAGGATGTAATAAACTCATTTGCAGTCACCGGAAAATCCATGGAAGAAGTGTTATGCATTTATTACACCATAGAAATGCTCTACATGTTAGAAACTCTGCATGACGTTGGCATCATTCATGGAGATTTCAAGCCTGATAATCTGCTTATTCGCTATTCTCGGGACTTCCCCACAAAAAATGGATTTCATGACAGAAATGAGCTTACAAAAGATGGATTTCGGGACAGAAGTGGCCCTTGGCTTGATCAGGGTCTTTGCCTTGTGGACTGGGGAAGAGGGATAGATCTGCGTCTCTTTCCTGACAATATGGAGTTTAAGGGAGATTGCCGAACTTCTGGATTTCGTTGTGTGGAGATGCAAGAGAATAAGCCTTGGACATTTCAGGTAGACACATATGGACTCTGTGTTGTTGTCCATATGATGCTGCATAATTCTTACATGGAGATTGACAAGAAACCATCACCTGATGGTGGTTACGCTTATCTACCCAAGGCATCTTTTAAAAGATACTGGAAGGTTGAGCTCTGGAAGAATTTCTTTGTGAAACTGCTTAACAGTAATCCTGGCGGCAATGACAAGAAATTGTTGCAAAATCTGAGGGAGTCCTTTCAGGAATACATGTGCTCGGACCCTCACCTTATAAAGAAACTAAGCGATTTACTGGCAAAGCAAAGGAAATCAATGTGTTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

394

Amino Acids

45.43

Weight (kDa)

8.36

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 81 - 307 3.3e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
Acc36I ACCTGC 1 cut(s) 248
AccI GTMKAC 2 cut(s) 454, 876
AciI CCGC 1 cut(s) 1056
AcsI RAATTY 2 cut(s) 356, 1021
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 2 cut(s) 232, 368
AfiI CCNNNNNNNGG 2 cut(s) 247, 959
AflIII ACRYGT 2 cut(s) 573, 1107
AgsI TTSAA 3 cut(s) 21, 193, 619
AjnI CCWGG 2 cut(s) 166, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 709, 741
AluBI AGCT 4 cut(s) 161, 411, 691, 1012
AluI AGCT 4 cut(s) 161, 411, 691, 1012
Alw21I GWGCWC 2 cut(s) 1014, 1115
Alw26I GTCTC 1 cut(s) 785
Ama87I CYCGRG 1 cut(s) 648
AoxI GGCC 1 cut(s) 721
ApeKI GCWGC 1 cut(s) 913
ApoI RAATTY 2 cut(s) 356, 1021
Asp700I GAANNNNTTC 2 cut(s) 48, 1022
AspS9I GGNCC 3 cut(s) 78, 722, 1117
AsuHPI GGTGA 4 cut(s) 53, 508, 942, 1115
AvaI CYCGRG 1 cut(s) 648
AvaII GGWCC 2 cut(s) 78, 1117
BanII GRGCYC 1 cut(s) 1014
BbsI GAAGAC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 1014, 1115
BbvI GCAGC 1 cut(s) 900
BccI CCATC 5 cut(s) 32, 131, 695, 950, 955
BciT130I CCWGG 2 cut(s) 168, 1053
BclI TGATCA 2 cut(s) 379, 733
BcoDI GTCTC 1 cut(s) 785
BfmI CTRYAG 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 248
BglII AGATCT 1 cut(s) 772
BisI GCNGC 2 cut(s) 914, 1057
BlsI GCNGC 2 cut(s) 915, 1058
Bme1390I CCNGG 2 cut(s) 168, 1053
Bme18I GGWCC 2 cut(s) 78, 1117
BmeT110I CYCGRG 1 cut(s) 648
BmgT120I GGNCC 3 cut(s) 78, 722, 1117
BmiI GGNNCC 1 cut(s) 1119
BmrFI CCNGG 2 cut(s) 168, 1053
BmrI ACTGGG 1 cut(s) 766
BmsI GCATC 5 cut(s) 442, 609, 834, 900, 992
BmuI ACTGGG 1 cut(s) 766
BpiI GAAGAC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 141
BsaJI CCNNGG 7 cut(s) 74, 166, 350, 528, 725, 860, 978
BsaWI WCCGGW 1 cut(s) 518
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 959
Bse1I ACTGG 4 cut(s) 81, 761, 1004, 1155
Bse3DI GCAATG 1 cut(s) 1066
Bse8I GATNNNNATC 1 cut(s) 141
BseBI CCWGG 2 cut(s) 168, 1053
BseDI CCNNGG 7 cut(s) 74, 166, 350, 528, 725, 860, 978
BseGI GGATG 1 cut(s) 499
BseJI GATNNNNATC 1 cut(s) 141
BseLI CCNNNNNNNGG 2 cut(s) 247, 959
BseMI GCAATG 1 cut(s) 1066
BseMII CTCAG 2 cut(s) 405, 1076
BseNI ACTGG 4 cut(s) 81, 761, 1004, 1155
BseRI GAGGAG 1 cut(s) 108
BseXI GCAGC 1 cut(s) 900
BshFI GGCC 1 cut(s) 723
BsiHKAI GWGCWC 2 cut(s) 1014, 1115
BsiHKCI CYCGRG 1 cut(s) 648
BsiSI CCGG 1 cut(s) 519
BslFI GGGAC 3 cut(s) 496, 665, 725
BslI CCNNNNNNNGG 2 cut(s) 247, 959
BsmAI GTCTC 1 cut(s) 785
BsmBI CGTCTC 1 cut(s) 785
BsmFI GGGAC 3 cut(s) 496, 665, 725
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 1 cut(s) 723
BsoBI CYCGRG 1 cut(s) 648
Bsp1286I GDGCHC 2 cut(s) 1014, 1115
Bsp143I GATC 3 cut(s) 379, 733, 772
Bsp19I CCATGG 2 cut(s) 74, 528
BspACI CCGC 1 cut(s) 1056
BspANI GGCC 1 cut(s) 723
BspCNI CTCAG 2 cut(s) 404, 1077
BspHI TCATGA 1 cut(s) 676
BspLI GGNNCC 1 cut(s) 1119
BspMI ACCTGC 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 1066
BsrI ACTGG 4 cut(s) 81, 761, 1004, 1155
BssECI CCNNGG 7 cut(s) 74, 166, 350, 528, 725, 860, 978
BssMI GATC 3 cut(s) 379, 733, 772
BssNAI GTATAC 1 cut(s) 455
BssT1I CCWWGG 5 cut(s) 74, 528, 725, 860, 978
Bst1107I GTATAC 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 168, 1053
Bst4CI ACNGT 4 cut(s) 302, 371, 449, 1046
Bst6I CTCTTC 1 cut(s) 757
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 391, 1085, 1139, 1182
BstDSI CCRYGG 3 cut(s) 74, 350, 528
BstF5I GGATG 1 cut(s) 499
BstKTI GATC 3 cut(s) 382, 736, 775
BstMAI GTCTC 1 cut(s) 785
BstMBI GATC 3 cut(s) 379, 733, 772
BstNI CCWGG 2 cut(s) 168, 1053
BstNSI RCATGY 2 cut(s) 577, 1111
BstSCI CCNGG 2 cut(s) 166, 1051
BstSFI CTRYAG 1 cut(s) 448
BstV1I GCAGC 1 cut(s) 900
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
BstZ17I GTATAC 1 cut(s) 455
BsuRI GGCC 1 cut(s) 723
BtgI CCRYGG 3 cut(s) 74, 350, 528
BtsCI GGATG 1 cut(s) 499
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 78, 722, 1117
CseI GACGC 1 cut(s) 767
Csp6I GTAC 2 cut(s) 231, 367
CviAII CATG 8 cut(s) 75, 529, 574, 590, 608, 677, 928, 1108
CviQI GTAC 2 cut(s) 231, 367
DdeI CTNAG 4 cut(s) 391, 1085, 1139, 1182
DpnI GATC 3 cut(s) 381, 735, 774
DpnII GATC 3 cut(s) 379, 733, 772
DraI TTTAAA 1 cut(s) 991
Eam1104I CTCTTC 1 cut(s) 757
EarI CTCTTC 1 cut(s) 757
Ecl136II GAGCTC 1 cut(s) 1012
Eco130I CCWWGG 5 cut(s) 74, 528, 725, 860, 978
Eco24I GRGCYC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 78, 1117
Eco53kI GAGCTC 1 cut(s) 1012
Eco57I CTGAAG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 648
EcoICRI GAGCTC 1 cut(s) 1012
EcoRI GAATTC 1 cut(s) 356
EcoRII CCWGG 2 cut(s) 166, 1051
EcoRV GATATC 1 cut(s) 143
EcoT14I CCWWGG 5 cut(s) 74, 528, 725, 860, 978
EcoT22I ATGCAT 2 cut(s) 435, 548
EcoT38I GRGCYC 1 cut(s) 1014
ErhI CCWWGG 5 cut(s) 74, 528, 725, 860, 978
Esp3I CGTCTC 1 cut(s) 785
FaeI CATG 8 cut(s) 78, 532, 577, 593, 611, 680, 931, 1111
FalI AAGNNNNNCTT 4 cut(s) 709, 741, 1010, 1042
FaqI GGGAC 3 cut(s) 496, 665, 725
FatI CATG 8 cut(s) 74, 528, 573, 589, 607, 676, 927, 1107
FauNDI CATATG 3 cut(s) 292, 883, 906
FbaI TGATCA 2 cut(s) 379, 733
FblI GTMKAC 2 cut(s) 454, 876
Fnu4HI GCNGC 2 cut(s) 914, 1057
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 1014
Fsp4HI GCNGC 2 cut(s) 914, 1057
GluI GCNGC 2 cut(s) 914, 1057
HaeIII GGCC 1 cut(s) 723
HapII CCGG 1 cut(s) 519
HgaI GACGC 1 cut(s) 767
Hin1II CATG 8 cut(s) 78, 532, 577, 593, 611, 680, 931, 1111
HincII GTYRAC 2 cut(s) 34, 298
HindII GTYRAC 2 cut(s) 34, 298
HindIII AAGCTT 2 cut(s) 159, 409
HinfI GANTC 4 cut(s) 44, 387, 888, 1091
HpaII CCGG 1 cut(s) 519
HphI GGTGA 4 cut(s) 53, 508, 942, 1115
Hpy166II GTNNAC 6 cut(s) 34, 298, 374, 455, 754, 877
Hpy188I TCNGA 5 cut(s) 7, 394, 445, 1086, 1117
Hpy188III TCNNGA 8 cut(s) 491, 650, 677, 710, 788, 825, 1015, 1100
Hpy8I GTNNAC 6 cut(s) 34, 298, 374, 455, 754, 877
HpyAV CCTTC 1 cut(s) 997
HpyCH4III ACNGT 4 cut(s) 302, 371, 449, 1046
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 9 cut(s) 323, 433, 465, 512, 546, 589, 847, 916, 1078
HpyF3I CTNAG 4 cut(s) 391, 1085, 1139, 1182
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 8 cut(s) 78, 532, 577, 593, 611, 680, 931, 1111
Ksp22I TGATCA 2 cut(s) 379, 733
Kzo9I GATC 3 cut(s) 379, 733, 772
Lsp1109I GCAGC 1 cut(s) 900
LweI GCATC 5 cut(s) 442, 609, 834, 900, 992
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 4 cut(s) 251, 302, 514, 962
MalI GATC 3 cut(s) 381, 735, 774
MboI GATC 3 cut(s) 379, 733, 772
MboII GAAGA 6 cut(s) 9, 177, 205, 545, 774, 1030
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 2 cut(s) 1014, 1115
MluCI AATT 6 cut(s) 8, 111, 356, 919, 1021, 1070
MlyI GAGTC 2 cut(s) 882, 1100
MnlI CCTC 4 cut(s) 129, 758, 1080, 1131
Mph1103I ATGCAT 2 cut(s) 435, 548
MroXI GAANNNNTTC 2 cut(s) 48, 1022
MseI TTAA 4 cut(s) 326, 804, 990, 1041
MslI CAYNNNNRTG 1 cut(s) 563
MspI CCGG 1 cut(s) 519
MspR9I CCNGG 2 cut(s) 168, 1053
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 2 cut(s) 168, 1053
NcoI CCATGG 2 cut(s) 74, 528
NdeI CATATG 3 cut(s) 292, 883, 906
NdeII GATC 3 cut(s) 379, 733, 772
NlaIII CATG 8 cut(s) 78, 532, 577, 593, 611, 680, 931, 1111
NlaIV GGNNCC 1 cut(s) 1119
NmuCI GTSAC 1 cut(s) 514
NsiI ATGCAT 2 cut(s) 435, 548
NspI RCATGY 2 cut(s) 577, 1111
PagI TCATGA 1 cut(s) 676
PciI ACATGT 2 cut(s) 573, 1107
PctI GAATGC 1 cut(s) 435
PdmI GAANNNNTTC 2 cut(s) 48, 1022
PfeI GAWTC 2 cut(s) 44, 387
PkrI GCNGC 2 cut(s) 915, 1058
PleI GAGTC 2 cut(s) 882, 1099
PpsI GAGTC 2 cut(s) 882, 1099
PscI ACATGT 2 cut(s) 573, 1107
PsiI TTATAA 1 cut(s) 1130
Psp124BI GAGCTC 1 cut(s) 1014
Psp6I CCWGG 2 cut(s) 166, 1051
PspGI CCWGG 2 cut(s) 166, 1051
PspN4I GGNNCC 1 cut(s) 1119
PspPI GGNCC 3 cut(s) 78, 722, 1117
PsuI RGATCY 1 cut(s) 772
RsaI GTAC 2 cut(s) 232, 368
RsaNI GTAC 2 cut(s) 231, 367
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 1014
SaqAI TTAA 4 cut(s) 326, 804, 990, 1041
SatI GCNGC 2 cut(s) 914, 1057
Sau3AI GATC 3 cut(s) 379, 733, 772
Sau96I GGNCC 3 cut(s) 78, 722, 1117
SchI GAGTC 2 cut(s) 882, 1100
ScrFI CCNGG 2 cut(s) 168, 1053
SduI GDGCHC 2 cut(s) 1014, 1115
SfaNI GCATC 5 cut(s) 442, 609, 834, 900, 992
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 2 cut(s) 78, 1117
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 276
SmoI CTYRAG 1 cut(s) 276
Sse9I AATT 6 cut(s) 8, 111, 356, 919, 1021, 1070
SsiI CCGC 1 cut(s) 1056
SstI GAGCTC 1 cut(s) 1014
StyD4I CCNGG 2 cut(s) 166, 1051
StyI CCWWGG 5 cut(s) 74, 528, 725, 860, 978
TaaI ACNGT 4 cut(s) 302, 371, 449, 1046
TaiI ACGT 1 cut(s) 597
TasI AATT 6 cut(s) 8, 111, 356, 919, 1021, 1070
TauI GCSGC 1 cut(s) 1059
TfiI GAWTC 2 cut(s) 44, 387
Tru1I TTAA 4 cut(s) 326, 804, 990, 1041
Tru9I TTAA 4 cut(s) 326, 804, 990, 1041
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 1 cut(s) 913
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 4 cut(s) 107, 143, 596, 665
VpaK11BI GGWCC 2 cut(s) 78, 1117
XapI RAATTY 2 cut(s) 356, 1021
XceI RCATGY 2 cut(s) 577, 1111
XmiI GTMKAC 2 cut(s) 454, 876
XmnI GAANNNNTTC 2 cut(s) 48, 1022
Zsp2I ATGCAT 2 cut(s) 435, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.