pycom09g16270

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006101
Physical Location & Seq
Forward (+)
16464429 .. 16466051
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g16270.2

Sequence Viewer

Length: 681 bp
ATGTTCACTGCCCACACTGTACGTCGTCCGAAGTACCAGATCACTGGTTGTGCAGGCCAGGGTGGCTTTGCTCAAGTACTCAAGGCATATGTCAACTGTAATCCTGAAGATGTTGTTGTTGCATTGAAGCCTGCTTTCCCATGGGAGTTTTATATGTATTATTGTATCATCAACTTGATCAACGGATCTCAGGCAAGGAATTCAAGCTTTGGTTTCGCTCATAGAATGCATCTCTATCCTGACTGTAGCATCCTTGTCTCCTACTATCTTGCTAGTGGAACACTTCAGGAATGGCCTTACAAAAGCAGGATCGATCGTGATAGAATTGACTTCACAGAAGAAGAATTTTGTGAGCGAAGTGGTCCGTGGCTTGATCAGGGTCTATGCCTAGTCGACTGGGGAAGAGGGATAGATCTCCATCTCTTTCCTGACAATGTGGAGTTTACAGGAGATTGCCGGACTTCCAGATTTCATTGTGTGGAAATGCAAGAAAATAGGCCATGGACATATCAAGTAGACACATATGGCCTCTGTGTTGTTGTCCATACTATGCTGCATAATTCTTACATGGAAATTGACAAGAAACCATCACCCGAGGTGGACGCTTCTATCTGCCCAAGTCATCTTTTAAAAGGTAGACACATATGGCCTCTGTGTTGTTGTGCATACTATGCTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

227

Amino Acids

26.1

Weight (kDa)

5.96

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 393, 516, 637
AclWI GGATC 2 cut(s) 193, 317
AcsI RAATTY 2 cut(s) 199, 344
AcuI CTGAAG 2 cut(s) 126, 269
AfaI GTAC 3 cut(s) 21, 35, 78
AgsI TTSAA 2 cut(s) 127, 204
AjnI CCWGG 1 cut(s) 57
AluBI AGCT 1 cut(s) 207
AluI AGCT 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 2 cut(s) 193, 317
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 5 cut(s) 55, 293, 497, 526, 647
ApeKI GCWGC 2 cut(s) 553, 674
ApoI RAATTY 2 cut(s) 199, 344
AspS9I GGNCC 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 582
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 362
BbvI GCAGC 2 cut(s) 540, 661
BccI CCATC 2 cut(s) 426, 595
BciT130I CCWGG 1 cut(s) 59
BclI TGATCA 2 cut(s) 177, 373
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 2 cut(s) 273, 389
BfmI CTRYAG 1 cut(s) 244
BglI GCCNNNNNGGC 1 cut(s) 63
BglII AGATCT 1 cut(s) 412
BisI GCNGC 2 cut(s) 554, 675
BlsI GCNGC 2 cut(s) 555, 676
BmcAI AGTACT 1 cut(s) 78
Bme1390I CCNGG 1 cut(s) 59
Bme18I GGWCC 1 cut(s) 362
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 362
BmrFI CCNGG 1 cut(s) 59
BmrI ACTGGG 1 cut(s) 406
BmsI GCATC 2 cut(s) 238, 258
BmuI ACTGGG 1 cut(s) 406
BpuEI CTTGAG 2 cut(s) 57, 65
Bsa29I ATCGAT 1 cut(s) 312
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 5 cut(s) 58, 140, 365, 500, 594
Bse1I ACTGG 2 cut(s) 49, 401
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 1 cut(s) 59
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 5 cut(s) 58, 140, 365, 500, 594
BseGI GGATG 1 cut(s) 249
BseJI GATNNNNATC 1 cut(s) 417
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 2 cut(s) 49, 401
BseXI GCAGC 2 cut(s) 540, 661
BsgI GTGCAG 1 cut(s) 72
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 5 cut(s) 57, 295, 499, 528, 649
BshVI ATCGAT 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 1 cut(s) 457
BsmAI GTCTC 1 cut(s) 262
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 5 cut(s) 57, 295, 499, 528, 649
BsoBI CYCGRG 1 cut(s) 593
Bsp143I GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
Bsp19I CCATGG 2 cut(s) 140, 500
BspANI GGCC 5 cut(s) 57, 295, 499, 528, 649
BspCNI CTCAG 1 cut(s) 202
BspDI ATCGAT 1 cut(s) 312
BspPI GGATC 2 cut(s) 193, 317
BsrI ACTGG 2 cut(s) 49, 401
BssECI CCNNGG 5 cut(s) 58, 140, 365, 500, 594
BssMI GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
BssT1I CCWWGG 2 cut(s) 140, 500
Bst2UI CCWGG 1 cut(s) 59
Bst4CI ACNGT 3 cut(s) 19, 98, 245
Bst6I CTCTTC 1 cut(s) 397
BstAPI GCANNNNNTGC 1 cut(s) 671
BstC8I GCNNGC 2 cut(s) 55, 132
BstDEI CTNAG 1 cut(s) 189
BstDSI CCRYGG 3 cut(s) 140, 365, 500
BstF5I GGATG 1 cut(s) 249
BstKTI GATC 7 cut(s) 42, 180, 188, 312, 316, 376, 415
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 2 cut(s) 63, 671
BstNI CCWGG 1 cut(s) 59
BstSCI CCNGG 1 cut(s) 57
BstSFI CTRYAG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 540, 661
BstX2I RGATCY 2 cut(s) 185, 412
BstXI CCANNNNNNTGG 1 cut(s) 44
BstYI RGATCY 2 cut(s) 185, 412
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 5 cut(s) 57, 295, 499, 528, 649
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 3 cut(s) 140, 365, 500
BtsCI GGATG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 3 cut(s) 6, 15, 42
Cac8I GCNNGC 2 cut(s) 55, 132
Cfr13I GGNCC 1 cut(s) 362
ClaI ATCGAT 1 cut(s) 312
CseI GACGC 1 cut(s) 611
Csp6I GTAC 3 cut(s) 20, 34, 77
CviAII CATG 3 cut(s) 141, 501, 568
CviJI RGCY 9 cut(s) 57, 66, 130, 207, 295, 370, 499, 528, 649
CviKI_1 RGCY 9 cut(s) 57, 66, 130, 207, 295, 370, 499, 528, 649
CviQI GTAC 3 cut(s) 20, 34, 77
DdeI CTNAG 1 cut(s) 189
DpnI GATC 7 cut(s) 41, 179, 187, 311, 315, 375, 414
DpnII GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
DraI TTTAAA 1 cut(s) 630
Eam1104I CTCTTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 140, 500
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 126, 269
Eco88I CYCGRG 1 cut(s) 593
EcoRI GAATTC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 57
EcoT14I CCWWGG 2 cut(s) 140, 500
EcoT22I ATGCAT 1 cut(s) 231
ErhI CCWWGG 2 cut(s) 140, 500
FaeI CATG 3 cut(s) 144, 504, 571
FatI CATG 3 cut(s) 140, 500, 567
FauNDI CATATG 3 cut(s) 88, 523, 644
FbaI TGATCA 2 cut(s) 177, 373
FblI GTMKAC 3 cut(s) 393, 516, 637
Fnu4HI GCNGC 2 cut(s) 554, 675
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 554, 675
FspBI CTAG 2 cut(s) 273, 389
GluI GCNGC 2 cut(s) 554, 675
HaeIII GGCC 5 cut(s) 57, 295, 499, 528, 649
HapII CCGG 1 cut(s) 457
HgaI GACGC 1 cut(s) 611
Hin1II CATG 3 cut(s) 144, 504, 571
HincII GTYRAC 2 cut(s) 94, 394
HindII GTYRAC 2 cut(s) 94, 394
HindIII AAGCTT 1 cut(s) 205
HpaII CCGG 1 cut(s) 457
HphI GGTGA 1 cut(s) 582
Hpy166II GTNNAC 7 cut(s) 6, 94, 394, 444, 517, 601, 638
Hpy188I TCNGA 1 cut(s) 30
Hpy188III TCNNGA 6 cut(s) 104, 239, 287, 317, 428, 465
Hpy8I GTNNAC 7 cut(s) 6, 94, 394, 444, 517, 601, 638
Hpy99I CGWCG 1 cut(s) 27
HpyCH4III ACNGT 3 cut(s) 19, 98, 245
HpyCH4IV ACGT 1 cut(s) 22
HpyCH4V TGCA 7 cut(s) 53, 122, 229, 487, 556, 665, 677
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 671
HpyF3I CTNAG 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 22
Hsp92II CATG 3 cut(s) 144, 504, 571
Ksp22I TGATCA 2 cut(s) 177, 373
Kzo9I GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
Lsp1109I GCAGC 2 cut(s) 540, 661
LweI GCATC 2 cut(s) 238, 258
MaeI CTAG 2 cut(s) 273, 389
MaeII ACGT 1 cut(s) 22
MalI GATC 7 cut(s) 41, 179, 187, 311, 315, 375, 414
MboI GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
MboII GAAGA 4 cut(s) 119, 350, 353, 414
MflI RGATCY 2 cut(s) 185, 412
MluCI AATT 5 cut(s) 199, 324, 344, 559, 573
MnlI CCTC 4 cut(s) 398, 539, 589, 660
Mph1103I ATGCAT 1 cut(s) 231
MseI TTAA 1 cut(s) 629
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 63, 671
NcoI CCATGG 2 cut(s) 140, 500
NdeI CATATG 3 cut(s) 88, 523, 644
NdeII GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
NlaIII CATG 3 cut(s) 144, 504, 571
NsiI ATGCAT 1 cut(s) 231
PctI GAATGC 1 cut(s) 231
PkrI GCNGC 2 cut(s) 555, 676
Ple19I CGATCG 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 57
PspGI CCWGG 1 cut(s) 57
PspPI GGNCC 1 cut(s) 362
PsuI RGATCY 2 cut(s) 185, 412
PvuI CGATCG 1 cut(s) 316
RsaI GTAC 3 cut(s) 21, 35, 78
RsaNI GTAC 3 cut(s) 20, 34, 77
SalI GTCGAC 1 cut(s) 392
SaqAI TTAA 1 cut(s) 629
SatI GCNGC 2 cut(s) 554, 675
Sau3AI GATC 7 cut(s) 39, 177, 185, 309, 313, 373, 412
Sau96I GGNCC 1 cut(s) 362
ScaI AGTACT 1 cut(s) 78
ScrFI CCNGG 1 cut(s) 59
SetI ASST 4 cut(s) 25, 209, 600, 637
SfaNI GCATC 2 cut(s) 238, 258
SfcI CTRYAG 1 cut(s) 244
SinI GGWCC 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 72, 80
SmoI CTYRAG 2 cut(s) 72, 80
Sse9I AATT 5 cut(s) 199, 324, 344, 559, 573
SspMI CTAG 2 cut(s) 273, 389
StyD4I CCNGG 1 cut(s) 57
StyI CCWWGG 2 cut(s) 140, 500
TaaI ACNGT 3 cut(s) 19, 98, 245
TaiI ACGT 1 cut(s) 25
TaqI TCGA 2 cut(s) 312, 393
TasI AATT 5 cut(s) 199, 324, 344, 559, 573
TatI WGTACW 1 cut(s) 76
Tru1I TTAA 1 cut(s) 629
Tru9I TTAA 1 cut(s) 629
TscAI CASTG 3 cut(s) 13, 22, 49
TseI GCWGC 2 cut(s) 553, 674
TspDTI ATGAA 1 cut(s) 461
TspGWI ACGGA 2 cut(s) 198, 354
TspRI CASTG 3 cut(s) 13, 22, 49
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 2 cut(s) 199, 344
XmiI GTMKAC 3 cut(s) 393, 516, 637
XspI CTAG 2 cut(s) 273, 389
ZrmI AGTACT 1 cut(s) 78
Zsp2I ATGCAT 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.