MD17G1236100.v1.1

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0006101
Physical Location & Seq
Forward (+)
28498142 .. 28502763
4622 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1236100.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGGACTCCGACACCACTCCTCTCCACGACCCTCTCTTGCCCTGGCTCTGGTCCGTGAAGCAAGCCCTAGAAGATTTTCACTCCGGAAAGGATTCCGGGTTGGATCTCACCAAGCTTCTATCCGACTGCATCGCCACATTCAAGGACAATGCTCAGTACAGGGACGACATCAGATTTCTCAAGATTTGGTTCCTCTATATGGATACCAGTGAAGATTTCGAAAGCGTTTTTCTGGAAATGTTGCACAGGGAGATTTGTGTTGGCCACTCTTTGCTTTATGTCTGGTACGCGATCTTTCTCGAAGCCAAAGAGAAATTGTACGATGCACACATGGTTTATCAGATGGGTATTTCAAGGAATGCTAAGCCAGTTGAGTGGGTGGAGAAGGCGCATGCCTTATTTCTTGCTCGGATGTATAGAATAGCTTCTTCAGCTCAGAAGATTGGAAAAGATGAAGCAGTTCCTTTTGGGAGCCTCATCAACCCATGGTCCAGCTCCACCATGAAAGACCTATTGAAGAAAGTTAATCGTCAACTTACAAAATATGATGGATACCATTCTACTACTAAAGCTTACTCGGGAAAAGTGGCTTTGTCTTCTCTCAAGAACTCGTCCAGGAACAAAACTATTGAGATAGGTCGTACGAAGTACCAGATCACTGGTTGTGCAGGTCAGGGTGGCTTTGCTCAAGTATATAAAGCATATGTCAATTGTAATCCTGAAGATGTTGTTGCATTGAAGATACAAAAGCCTGCTTTCCCTTGGGAGTTCTATATGTATCGTCAACTTGATCAACGGATCTCAGACAAGGAAAGGTCAAGCTTTGGGTTTGCTCATAGAATGCATCTCTATCCCGACTGTAGCATTCTAGTCTCCGACTATCTTGCTAGTGGAACACTTCAGGATGCAATAAATTCATATGCCTTCAAAGGCAAGTCCATGGAAGAAGTGTTATGCATTTATTACACCATAGAAATGCTCTACATGCTAGAAACTCTGCATGGTGTTGGCATCATTCATGGAGATTTCAAGCCTGATAATTTGCTTATTCGTTATGCTAGGAATGGCCTTACAAAAGCCGGATCTTGTGACAGAACTGACTTTACAGAAGAAGAATTTCGTGAGAGAAGTGGTCCGTGGCTTGATCAGGGTCTATGCCTTGTCGACTGGGGAAGAGGGATAGATCTGCATCTCTTTCCTGACAATGTGGAGTTTACGGGAGATTGCCGGACTTCTGGATTTCGTTGTGTGGAAATGCAAGAGAATAGGCCATGGACATATCAAGTAGACACATACGGACTCTGTGTTGTTGTCCACACGATGCTGCATAATTCTTACATGGAAATTGACAAGAAACCATCACCCGAAGGTGGTCACTTCTATCTGCCCAAGTCATCTTTTAAAAGATACTGGAATGTCGAACTCTGGAGGAACTTCTTTAAGAAACTGCTTAATAGTAGCCCCGGCTGTAACCACAAGAAGCTGTTGCAGGATCTGAGGGAGTCCTTCCAGGATTACTTGTGCTCGGAGCCTCAGCTTATAAGGAAACTAAGCGATTTACTGGTCAAGCAAAGGGCCTCACTGTGTTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

532

Amino Acids

61.38

Weight (kDa)

6.96

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 8 - 117 2.3e-24 Mad3/BUB1 homology region 1
Pkinase PF00069 220 - 444 5.3e-11 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1541
Acc36I ACCTGC 1 cut(s) 659
AccI GTMKAC 2 cut(s) 1164, 1287
AccII CGCG 1 cut(s) 290
AccIII TCCGGA 1 cut(s) 83
AclWI GGATC 4 cut(s) 111, 806, 1090, 1500
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 913, 1115
AcuI CTGAAG 3 cut(s) 414, 741, 884
AfaI GTAC 5 cut(s) 158, 287, 320, 643, 650
AfiI CCNNNNNNNGG 3 cut(s) 48, 199, 1370
AgsI TTSAA 6 cut(s) 142, 354, 517, 739, 928, 1030
AjnI CCWGG 3 cut(s) 41, 614, 1509
AluBI AGCT 8 cut(s) 115, 425, 434, 495, 572, 822, 1483, 1537
AluI AGCT 8 cut(s) 115, 425, 434, 495, 572, 822, 1483, 1537
Alw21I GWGCWC 1 cut(s) 1526
Alw26I GTCTC 1 cut(s) 877
AlwI GGATC 4 cut(s) 111, 806, 1090, 1500
AlwNI CAGNNNCTG 1 cut(s) 1495
Ama87I CYCGRG 1 cut(s) 577
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 4 cut(s) 262, 1066, 1268, 1575
ApeKI GCWGC 1 cut(s) 1324
ApoI RAATTY 2 cut(s) 913, 1115
Asp700I GAANNNNTTC 5 cut(s) 75, 91, 424, 459, 1116
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 4 cut(s) 51, 489, 1133, 1575
AsuC2I CCSGG 2 cut(s) 97, 1464
AsuHPI GGTGA 2 cut(s) 100, 1353
AsuII TTCGAA 1 cut(s) 219
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 3 cut(s) 51, 489, 1133
BalI TGGCCA 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 1526
BbvCI CCTCAGC 1 cut(s) 1533
BbvI GCAGC 1 cut(s) 1311
BccI CCATC 3 cut(s) 337, 542, 1366
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BciT130I CCWGG 3 cut(s) 43, 616, 1511
BciVI GTATCC 2 cut(s) 196, 545
BclI TGATCA 2 cut(s) 790, 1144
BcnI CCSGG 2 cut(s) 97, 1464
BcoDI GTCTC 1 cut(s) 877
BfaI CTAG 5 cut(s) 68, 869, 888, 989, 1059
BfmI CTRYAG 1 cut(s) 859
BfuAI ACCTGC 1 cut(s) 659
BfuI GTATCC 2 cut(s) 196, 545
BglII AGATCT 1 cut(s) 1183
BisI GCNGC 1 cut(s) 1325
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 1326
Bme1390I CCNGG 5 cut(s) 43, 97, 616, 1464, 1511
Bme18I GGWCC 3 cut(s) 51, 489, 1133
BmeT110I CYCGRG 1 cut(s) 577
BmgT120I GGNCC 4 cut(s) 51, 489, 1133, 1575
BmiI GGNNCC 3 cut(s) 191, 473, 1530
BmrFI CCNGG 5 cut(s) 43, 97, 616, 1464, 1511
BmrI ACTGGG 1 cut(s) 1177
BmsI GCATC 7 cut(s) 138, 313, 853, 895, 1020, 1198, 1311
BmuI ACTGGG 1 cut(s) 1177
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 1 cut(s) 1447
Bpu10I CCTNAGC 1 cut(s) 1533
Bpu1102I GCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 219
BpuEI CTTGAG 3 cut(s) 164, 587, 672
BpuMI CCSGG 2 cut(s) 97, 1464
BsaBI GATNNNNATC 1 cut(s) 1188
BsaJI CCNNGG 7 cut(s) 41, 485, 761, 939, 1136, 1271, 1462
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 199, 1370
Bse1I ACTGG 6 cut(s) 207, 368, 664, 1172, 1415, 1566
Bse8I GATNNNNATC 1 cut(s) 1188
BseAI TCCGGA 1 cut(s) 83
BseBI CCWGG 3 cut(s) 43, 616, 1511
BseDI CCNNGG 7 cut(s) 41, 485, 761, 939, 1136, 1271, 1462
BseGI GGATG 2 cut(s) 417, 910
BseJI GATNNNNATC 1 cut(s) 1188
BseLI CCNNNNNNNGG 3 cut(s) 48, 199, 1370
BseMII CTCAG 5 cut(s) 167, 449, 816, 1487, 1547
BseNI ACTGG 6 cut(s) 207, 368, 664, 1172, 1415, 1566
BseRI GAGGAG 1 cut(s) 9
BseXI GCAGC 1 cut(s) 1311
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 1 cut(s) 290
BshFI GGCC 4 cut(s) 264, 1068, 1270, 1577
BsiHKAI GWGCWC 1 cut(s) 1526
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 5 cut(s) 84, 96, 1080, 1228, 1464
BsiWI CGTACG 1 cut(s) 641
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 3 cut(s) 48, 199, 1370
BsmAI GTCTC 1 cut(s) 877
BsmFI GGGAC 1 cut(s) 176
BsmI GAATGC 3 cut(s) 364, 846, 864
BsnI GGCC 4 cut(s) 264, 1068, 1270, 1577
BsoBI CYCGRG 1 cut(s) 577
Bsp119I TTCGAA 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 1526
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
Bsp1720I GCTNAGC 1 cut(s) 363
Bsp19I CCATGG 3 cut(s) 485, 939, 1271
BspANI GGCC 4 cut(s) 264, 1068, 1270, 1577
BspCNI CTCAG 5 cut(s) 166, 448, 815, 1488, 1546
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 3 cut(s) 191, 473, 1530
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 4 cut(s) 111, 806, 1090, 1500
BspT104I TTCGAA 1 cut(s) 219
BsrI ACTGG 6 cut(s) 207, 368, 664, 1172, 1415, 1566
BssECI CCNNGG 7 cut(s) 41, 485, 761, 939, 1136, 1271, 1462
BssMI GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
BssT1I CCWWGG 4 cut(s) 485, 761, 939, 1271
Bst2UI CCWGG 3 cut(s) 43, 616, 1511
Bst4CI ACNGT 2 cut(s) 860, 1584
Bst6I CTCTTC 1 cut(s) 1168
BstBI TTCGAA 1 cut(s) 219
BstC8I GCNNGC 3 cut(s) 63, 393, 753
BstDEI CTNAG 7 cut(s) 153, 363, 435, 802, 1496, 1533, 1550
BstDSI CCRYGG 4 cut(s) 485, 939, 1136, 1271
BstF5I GGATG 2 cut(s) 417, 910
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 9 cut(s) 106, 294, 657, 793, 801, 1085, 1147, 1186, 1495
BstMAI GTCTC 1 cut(s) 877
BstMBI GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
BstMWI GCNNNNNNNGC 2 cut(s) 431, 985
BstNI CCWGG 3 cut(s) 43, 616, 1511
BstNSI RCATGY 2 cut(s) 395, 988
BstSCI CCNGG 5 cut(s) 41, 95, 614, 1462, 1509
BstSFI CTRYAG 1 cut(s) 859
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 1 cut(s) 1311
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 5 cut(s) 103, 798, 1082, 1183, 1492
BstXI CCANNNNNNTGG 2 cut(s) 375, 659
BstYI RGATCY 5 cut(s) 103, 798, 1082, 1183, 1492
BsuI GTATCC 2 cut(s) 196, 545
BsuRI GGCC 4 cut(s) 264, 1068, 1270, 1577
BtgI CCRYGG 4 cut(s) 485, 939, 1136, 1271
BtgZI GCGATG 1 cut(s) 115
BtsCI GGATG 2 cut(s) 417, 910
BtsIMutI CAGTG 3 cut(s) 214, 657, 1580
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 3 cut(s) 63, 393, 753
CaiI CAGNNNCTG 1 cut(s) 1495
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 4 cut(s) 51, 489, 1133, 1575
Csp6I GTAC 5 cut(s) 157, 286, 319, 642, 649
CviQI GTAC 5 cut(s) 157, 286, 319, 642, 649
DdeI CTNAG 7 cut(s) 153, 363, 435, 802, 1496, 1533, 1550
DpnI GATC 9 cut(s) 105, 293, 656, 792, 800, 1084, 1146, 1185, 1494
DpnII GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
DraI TTTAAA 1 cut(s) 1402
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 1168
EarI CTCTTC 1 cut(s) 1168
Eco130I CCWWGG 4 cut(s) 485, 761, 939, 1271
Eco47I GGWCC 3 cut(s) 51, 489, 1133
Eco57I CTGAAG 3 cut(s) 414, 741, 884
Eco88I CYCGRG 1 cut(s) 577
EcoO109I RGGNCCY 1 cut(s) 1575
EcoRII CCWGG 3 cut(s) 41, 614, 1509
EcoT14I CCWWGG 4 cut(s) 485, 761, 939, 1271
EcoT22I ATGCAT 2 cut(s) 846, 959
ErhI CCWWGG 4 cut(s) 485, 761, 939, 1271
FaqI GGGAC 1 cut(s) 176
FauNDI CATATG 2 cut(s) 703, 919
FbaI TGATCA 2 cut(s) 790, 1144
FblI GTMKAC 2 cut(s) 1164, 1287
Fnu4HI GCNGC 1 cut(s) 1325
FokI GGATG 2 cut(s) 424, 917
Fsp4HI GCNGC 1 cut(s) 1325
FspBI CTAG 5 cut(s) 68, 869, 888, 989, 1059
GlaI GCGC 1 cut(s) 390
GluI GCNGC 1 cut(s) 1325
GsuI CTGGAG 1 cut(s) 1447
HaeIII GGCC 4 cut(s) 264, 1068, 1270, 1577
HapII CCGG 5 cut(s) 84, 96, 1080, 1228, 1464
HhaI GCGC 1 cut(s) 391
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 3 cut(s) 533, 785, 1165
HindII GTYRAC 3 cut(s) 533, 785, 1165
HindIII AAGCTT 3 cut(s) 113, 570, 820
HinfI GANTC 4 cut(s) 5, 92, 1299, 1502
HpaII CCGG 5 cut(s) 84, 96, 1080, 1228, 1464
HphI GGTGA 2 cut(s) 100, 1353
Hpy166II GTNNAC 6 cut(s) 533, 785, 1165, 1215, 1288, 1315
Hpy8I GTNNAC 6 cut(s) 533, 785, 1165, 1215, 1288, 1315
HpyAV CCTTC 4 cut(s) 379, 934, 1361, 1516
HpyCH4III ACNGT 2 cut(s) 860, 1584
HpyF10VI GCNNNNNNNGC 2 cut(s) 431, 985
HpyF3I CTNAG 7 cut(s) 153, 363, 435, 802, 1496, 1533, 1550
HspAI GCGC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 2 cut(s) 790, 1144
Kzo9I GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
LmnI GCTCC 3 cut(s) 471, 500, 1528
Lsp1109I GCAGC 1 cut(s) 1311
LweI GCATC 7 cut(s) 138, 313, 853, 895, 1020, 1198, 1311
MaeI CTAG 5 cut(s) 68, 869, 888, 989, 1059
MaeIII GTNAC 3 cut(s) 1088, 1373, 1469
MalI GATC 9 cut(s) 105, 293, 656, 792, 800, 1084, 1146, 1185, 1494
MboI GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
MfeI CAATTG 1 cut(s) 709
MflI RGATCY 5 cut(s) 103, 798, 1082, 1183, 1492
MhlI GDGCHC 1 cut(s) 1526
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 7 cut(s) 314, 709, 913, 1039, 1115, 1330, 1344
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 1293, 1511
MmeI TCCRAC 4 cut(s) 33, 81, 147, 900
MnlI CCTC 9 cut(s) 30, 42, 203, 485, 1169, 1422, 1491, 1542, 1588
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 2 cut(s) 846, 959
MroI TCCGGA 1 cut(s) 83
MroXI GAANNNNTTC 5 cut(s) 75, 91, 424, 459, 1116
MscI TGGCCA 1 cut(s) 264
MseI TTAA 5 cut(s) 525, 1401, 1440, 1452, 1594
MslI CAYNNNNRTG 1 cut(s) 974
Msp20I TGGCCA 1 cut(s) 264
MspI CCGG 5 cut(s) 84, 96, 1080, 1228, 1464
MspR9I CCNGG 5 cut(s) 43, 97, 616, 1464, 1511
MunI CAATTG 1 cut(s) 709
Mva1269I GAATGC 3 cut(s) 364, 846, 864
MvaI CCWGG 3 cut(s) 43, 616, 1511
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 2 cut(s) 431, 985
NciI CCSGG 2 cut(s) 97, 1464
NcoI CCATGG 3 cut(s) 485, 939, 1271
NdeI CATATG 2 cut(s) 703, 919
NdeII GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
NlaIV GGNNCC 3 cut(s) 191, 473, 1530
NmuCI GTSAC 2 cut(s) 1088, 1373
NsiI ATGCAT 2 cut(s) 846, 959
NspI RCATGY 2 cut(s) 395, 988
NspV TTCGAA 1 cut(s) 219
PaeI GCATGC 1 cut(s) 395
PctI GAATGC 3 cut(s) 364, 846, 864
PdmI GAANNNNTTC 5 cut(s) 75, 91, 424, 459, 1116
PfeI GAWTC 1 cut(s) 92
Pfl23II CGTACG 1 cut(s) 641
PfoI TCCNGGA 2 cut(s) 614, 1509
PkrI GCNGC 1 cut(s) 1326
PleI GAGTC 2 cut(s) 1293, 1510
PpsI GAGTC 2 cut(s) 1293, 1510
PsiI TTATAA 1 cut(s) 1541
Psp6I CCWGG 3 cut(s) 41, 614, 1509
PspGI CCWGG 3 cut(s) 41, 614, 1509
PspLI CGTACG 1 cut(s) 641
PspN4I GGNNCC 3 cut(s) 191, 473, 1530
PspPI GGNCC 4 cut(s) 51, 489, 1133, 1575
PsrI GAACNNNNNNTAC 2 cut(s) 1087, 1119
PstNI CAGNNNCTG 1 cut(s) 1495
PsuI RGATCY 5 cut(s) 103, 798, 1082, 1183, 1492
RsaI GTAC 5 cut(s) 158, 287, 320, 643, 650
RsaNI GTAC 5 cut(s) 157, 286, 319, 642, 649
RseI CAYNNNNRTG 1 cut(s) 974
SalI GTCGAC 1 cut(s) 1163
SaqAI TTAA 5 cut(s) 525, 1401, 1440, 1452, 1594
SatI GCNGC 1 cut(s) 1325
Sau3AI GATC 9 cut(s) 103, 291, 654, 790, 798, 1082, 1144, 1183, 1492
Sau96I GGNCC 4 cut(s) 51, 489, 1133, 1575
SchI GAGTC 2 cut(s) 1293, 1511
ScrFI CCNGG 5 cut(s) 43, 97, 616, 1464, 1511
SduI GDGCHC 1 cut(s) 1526
SfaNI GCATC 7 cut(s) 138, 313, 853, 895, 1020, 1198, 1311
SfcI CTRYAG 1 cut(s) 859
SfuI TTCGAA 1 cut(s) 219
SinI GGWCC 3 cut(s) 51, 489, 1133
SmiMI CAYNNNNRTG 1 cut(s) 974
SmlI CTYRAG 3 cut(s) 179, 602, 687
SmoI CTYRAG 3 cut(s) 179, 602, 687
SphI GCATGC 1 cut(s) 395
Sse9I AATT 7 cut(s) 314, 709, 913, 1039, 1115, 1330, 1344
SspMI CTAG 5 cut(s) 68, 869, 888, 989, 1059
StyD4I CCNGG 5 cut(s) 41, 95, 614, 1462, 1509
StyI CCWWGG 4 cut(s) 485, 761, 939, 1271
TaaI ACNGT 2 cut(s) 860, 1584
TaqI TCGA 4 cut(s) 219, 300, 1164, 1419
TasI AATT 7 cut(s) 314, 709, 913, 1039, 1115, 1330, 1344
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 1 cut(s) 92
Tru1I TTAA 5 cut(s) 525, 1401, 1440, 1452, 1594
Tru9I TTAA 5 cut(s) 525, 1401, 1440, 1452, 1594
TscAI CASTG 3 cut(s) 214, 664, 1587
TseFI GTSAC 2 cut(s) 1088, 1373
TseI GCWGC 1 cut(s) 1324
Tsp45I GTSAC 2 cut(s) 1088, 1373
TspDTI ATGAA 4 cut(s) 468, 518, 906, 1007
TspGWI ACGGA 4 cut(s) 43, 811, 1125, 1311
TspRI CASTG 3 cut(s) 214, 664, 1587
VpaK11BI GGWCC 3 cut(s) 51, 489, 1133
XapI RAATTY 2 cut(s) 913, 1115
XceI RCATGY 2 cut(s) 395, 988
XmiI GTMKAC 2 cut(s) 1164, 1287
XmnI GAANNNNTTC 5 cut(s) 75, 91, 424, 459, 1116
XspI CTAG 5 cut(s) 68, 869, 888, 989, 1059
Zsp2I ATGCAT 2 cut(s) 846, 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.