Rroxscaffold_2G00116620

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006101
Physical Location & Seq
Forward (+)
43866715 .. 43874005
7291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116620.1

Sequence Viewer

Length: 1632 bp
ATGGCTGCCGTCTTCCAACACTCGCCGGCCACCGCCATTCCTCTTCACGACCCTCTCTTGCCCTCCCTCCGGTTGATAAAGCAAGCACTCGATGACTTCAACTCCGGAAACCATTCCGGGTCGGATCTCACTAACCTTCTATCCGATTGCATCGCAGCTTTCAAGGACAATGTTCAGTACCGAGACGACGCCAGATTCCTCAAGATTTGGTTCCTCTATATGGGGTTTATTGAAGATTTCGAAAGCTTGTTTATGGAAATGTTGGATAGAAAGATATGTATCGGACATTCTTTGCTTTATGTTTGGTATGCCTCCTTTCTAGAGTCCAAAGGCAAGTTATACGATGCGCACATGGTTTATCAGATGGGTATTTCAAGGAATGCTAAGCCTGCTGAGTGGGTGAAGAAAGCACATGGCTTATTTCTTGAGAGGATGTCTGAATTAGTTTCTTCAACTCACAAGGTTGACGACAGTGAATCCATTCAGTTTGACAGCCACATCAATCCATGGTCCAGTTCCACAATGAAAGCCCTATTAAAGAAAATTACTCCTCAACTTATGAAATACGATGGATATCACTCCACTACTAAAGCTTACCCAGGAAAAGTGGCATTGTCTTCTTTGAAGAACTCATCAAGGAACAAGATTATTGAGATAGGTCGTACAAAATACCAGATTACGGGTTACGCAGGTCAAGGTGGTTTTGCTCAAGTATATAAAGCATATGTCAACTGTAACCCTGATGATGTTGTTGCATTAAAGATACAAAAGCCTGCTTTCCCGTGGGAATTCTATATGTACCGTGAACTTGATCAACGAATCTCAGACAAGGAAAGGTCAAGCTTTGGTTTCGCTCAAAGAATGCATCTCTATTCTGACTGTAGTATACTCGTCTGCAACTATATTGCTAATGGGACACTTCAGGATGTAATAAACTCATTTGCAGTCACCGGAAAATCCATGGAAGAAGTGTTATGCATTTATTACACCATAGAAATGCTCTACATGTTAGAAACTCTGCATGACGTTGGCATCATTCATGGAGATTTCAAGCCTGATAATCTGCTTATTCGCTATTCTCGGGACTTCCCCACAAAAAATGGATTTCATGACAGAAATGAGCTTACAAAAGATGGATTTCAGGACAGAAGTGGCCCTTGGCTTGATCAGGGTCTTTGCCTTGTGGACTGGGGAAGAGGGATAGATCAGCGTCTCTTTCCTGACAATATGGAGTTTAAGGGAGATTGCCGAACTTCTGGATTTCGTTGTGTGGAGATGCAAGAGAATAAGCCTTGGACATTTCAGGCTAGTTCGCATTTCGTAGACACATATGGACTCTGTGTTGTTGTCCATATGATGCTGCATAATTCTTACATGGAGATTGACAAGAAACCATCACCTGATGGTGGTTACGCTTATCTACCCAAGGCATCTTTTAAAAGATACTGGAAGGTTGAGCTCTGGAAGAATTTCTTTGTGAAACTGCTTAACAGTAATCCTGGCTGCAATGACAAGAAATTGTTGCAAAATCTGAGGAGTCCTTTCAGGAATACATTGTGCCTTTCATCTCTCCTGATTATCTCTTATAGGGTATTACGCACGGTCTCTCGTTCAGGCAAGTGCAACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

543

Amino Acids

62.53

Weight (kDa)

8.59

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 16 - 124 1.9e-24 Mad3/BUB1 homology region 1
Pkinase PF00069 225 - 360 1.5e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 348
Acc36I ACCTGC 1 cut(s) 680
AccI GTMKAC 2 cut(s) 886, 1323
AccIII TCCGGA 1 cut(s) 104
AciI CCGC 1 cut(s) 33
AclWI GGATC 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 2 cut(s) 788, 1468
AcuI CTGAAG 1 cut(s) 905
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 3 cut(s) 179, 664, 800
AfiI CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1406
AflIII ACRYGT 1 cut(s) 1005
AgeI ACCGGT 1 cut(s) 1626
AgsI TTSAA 7 cut(s) 100, 163, 233, 375, 453, 625, 1051
AjnI CCWGG 2 cut(s) 598, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 1141, 1173
AluBI AGCT 6 cut(s) 158, 246, 593, 843, 1123, 1459
AluI AGCT 6 cut(s) 158, 246, 593, 843, 1123, 1459
Alw21I GWGCWC 1 cut(s) 1461
Alw26I GTCTC 3 cut(s) 177, 1217, 1609
AlwI GGATC 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 1080
Aor13HI TCCGGA 1 cut(s) 104
AoxI GGCC 2 cut(s) 27, 1153
ApeKI GCWGC 4 cut(s) 5, 155, 1360, 1503
ApoI RAATTY 2 cut(s) 788, 1468
AsiGI ACCGGT 1 cut(s) 1626
Asp700I GAANNNNTTC 3 cut(s) 112, 480, 1469
AspLEI GCGC 1 cut(s) 349
AspS9I GGNCC 2 cut(s) 510, 1154
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 3 cut(s) 412, 940, 1389
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 1080
AvaII GGWCC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 1461
BbsI GAAGAC 2 cut(s) 4, 609
Bbv12I GWGCWC 1 cut(s) 1461
BbvI GCAGC 3 cut(s) 167, 1347, 1490
BccI CCATC 5 cut(s) 358, 563, 1127, 1397, 1402
BciT130I CCWGG 2 cut(s) 600, 1500
BclI TGATCA 2 cut(s) 811, 1165
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 3 cut(s) 177, 1217, 1609
BfaI CTAG 2 cut(s) 320, 1308
BfmI CTRYAG 1 cut(s) 880
BfuAI ACCTGC 1 cut(s) 680
BisI GCNGC 4 cut(s) 6, 156, 1361, 1504
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 4 cut(s) 7, 157, 1362, 1505
Bme1390I CCNGG 3 cut(s) 118, 600, 1500
Bme18I GGWCC 1 cut(s) 510
BmeT110I CYCGRG 1 cut(s) 1080
BmgT120I GGNCC 2 cut(s) 510, 1154
BmiI GGNNCC 1 cut(s) 212
BmrFI CCNGG 3 cut(s) 118, 600, 1500
BmrI ACTGGG 1 cut(s) 1198
BmsI GCATC 7 cut(s) 159, 334, 874, 1041, 1266, 1347, 1439
BmuI ACTGGG 1 cut(s) 1198
BpiI GAAGAC 2 cut(s) 4, 609
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 185, 446, 693
BpuMI CCSGG 1 cut(s) 118
BsaBI GATNNNNATC 2 cut(s) 278, 573
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 1609
BsaJI CCNNGG 7 cut(s) 506, 598, 782, 960, 1157, 1292, 1425
BsaWI WCCGGW 4 cut(s) 69, 104, 950, 1626
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1406
Bse118I RCCGGY 2 cut(s) 25, 1626
Bse1I ACTGG 3 cut(s) 513, 1193, 1451
Bse3DI GCAATG 1 cut(s) 1513
Bse8I GATNNNNATC 2 cut(s) 278, 573
BseAI TCCGGA 1 cut(s) 104
BseBI CCWGG 2 cut(s) 600, 1500
BseDI CCNNGG 7 cut(s) 506, 598, 782, 960, 1157, 1292, 1425
BseGI GGATG 2 cut(s) 438, 931
BseJI GATNNNNATC 2 cut(s) 278, 573
BseLI CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1406
BseMI GCAATG 1 cut(s) 1513
BseMII CTCAG 3 cut(s) 384, 837, 1523
BseNI ACTGG 3 cut(s) 513, 1193, 1451
BseRI GAGGAG 2 cut(s) 540, 1549
BseXI GCAGC 3 cut(s) 167, 1347, 1490
BshFI GGCC 2 cut(s) 29, 1155
BshTI ACCGGT 1 cut(s) 1626
BsiHKAI GWGCWC 1 cut(s) 1461
BsiHKCI CYCGRG 1 cut(s) 1080
BsiSI CCGG 6 cut(s) 26, 70, 105, 117, 951, 1627
BslFI GGGAC 2 cut(s) 928, 1097
BslI CCNNNNNNNGG 4 cut(s) 69, 220, 679, 1406
BsmAI GTCTC 3 cut(s) 177, 1217, 1609
BsmBI CGTCTC 2 cut(s) 177, 1217
BsmFI GGGAC 2 cut(s) 928, 1097
BsmI GAATGC 2 cut(s) 385, 867
BsnI GGCC 2 cut(s) 29, 1155
Bso31I GGTCTC 1 cut(s) 1609
BsoBI CYCGRG 1 cut(s) 1080
Bsp119I TTCGAA 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 1461
Bsp13I TCCGGA 1 cut(s) 104
Bsp143I GATC 4 cut(s) 124, 811, 1165, 1204
Bsp1720I GCTNAGC 1 cut(s) 384
Bsp19I CCATGG 2 cut(s) 506, 960
BspACI CCGC 1 cut(s) 33
BspANI GGCC 2 cut(s) 29, 1155
BspCNI CTCAG 3 cut(s) 385, 836, 1524
BspEI TCCGGA 1 cut(s) 104
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 1 cut(s) 212
BspMI ACCTGC 1 cut(s) 680
BspPI GGATC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 240
BspTNI GGTCTC 1 cut(s) 1609
BsrDI GCAATG 1 cut(s) 1513
BsrFI RCCGGY 2 cut(s) 25, 1626
BsrI ACTGG 3 cut(s) 513, 1193, 1451
BssAI RCCGGY 2 cut(s) 25, 1626
BssECI CCNNGG 7 cut(s) 506, 598, 782, 960, 1157, 1292, 1425
BssMI GATC 4 cut(s) 124, 811, 1165, 1204
BssNAI GTATAC 1 cut(s) 887
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 5 cut(s) 506, 960, 1157, 1292, 1425
Bst1107I GTATAC 1 cut(s) 887
Bst2UI CCWGG 2 cut(s) 600, 1500
Bst4CI ACNGT 6 cut(s) 473, 734, 803, 881, 1493, 1603
Bst6I CTCTTC 2 cut(s) 48, 1189
BstACI GRCGYC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 4 cut(s) 27, 84, 390, 774
BstDEI CTNAG 4 cut(s) 384, 393, 823, 1532
BstDSI CCRYGG 3 cut(s) 506, 782, 960
BstF5I GGATG 2 cut(s) 438, 931
BstHHI GCGC 1 cut(s) 349
BstKTI GATC 4 cut(s) 127, 814, 1168, 1207
BstMAI GTCTC 3 cut(s) 177, 1217, 1609
BstMBI GATC 4 cut(s) 124, 811, 1165, 1204
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 2 cut(s) 600, 1500
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 3 cut(s) 116, 598, 1498
BstSFI CTRYAG 1 cut(s) 880
BstV1I GCAGC 3 cut(s) 167, 1347, 1490
BstV2I GAAGAC 2 cut(s) 4, 609
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 887
BsuRI GGCC 2 cut(s) 29, 1155
BtgI CCRYGG 3 cut(s) 506, 782, 960
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 2 cut(s) 438, 931
BtsIMutI CAGTG 1 cut(s) 478
BveI ACCTGC 1 cut(s) 680
Cac8I GCNNGC 4 cut(s) 27, 84, 390, 774
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 1 cut(s) 349
Cfr10I RCCGGY 2 cut(s) 25, 1626
Cfr13I GGNCC 2 cut(s) 510, 1154
CseI GACGC 2 cut(s) 197, 1199
Csp6I GTAC 3 cut(s) 178, 663, 799
CspAI ACCGGT 1 cut(s) 1626
CviAII CATG 9 cut(s) 352, 413, 507, 961, 1006, 1022, 1040, 1109, 1375
CviQI GTAC 3 cut(s) 178, 663, 799
DdeI CTNAG 4 cut(s) 384, 393, 823, 1532
DpnI GATC 4 cut(s) 126, 813, 1167, 1206
DpnII GATC 4 cut(s) 124, 811, 1165, 1204
DraI TTTAAA 1 cut(s) 1438
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 48, 1189
EarI CTCTTC 2 cut(s) 48, 1189
Ecl136II GAGCTC 1 cut(s) 1459
Eco130I CCWWGG 5 cut(s) 506, 960, 1157, 1292, 1425
Eco24I GRGCYC 1 cut(s) 1461
Eco31I GGTCTC 1 cut(s) 1609
Eco32I GATATC 1 cut(s) 575
Eco47I GGWCC 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 1459
Eco57I CTGAAG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 1080
EcoICRI GAGCTC 1 cut(s) 1459
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 2 cut(s) 598, 1498
EcoRV GATATC 1 cut(s) 575
EcoT14I CCWWGG 5 cut(s) 506, 960, 1157, 1292, 1425
EcoT22I ATGCAT 2 cut(s) 867, 980
EcoT38I GRGCYC 1 cut(s) 1461
ErhI CCWWGG 5 cut(s) 506, 960, 1157, 1292, 1425
Esp3I CGTCTC 2 cut(s) 177, 1217
FaeI CATG 9 cut(s) 355, 416, 510, 964, 1009, 1025, 1043, 1112, 1378
FalI AAGNNNNNCTT 4 cut(s) 1141, 1173, 1457, 1489
FaqI GGGAC 2 cut(s) 928, 1097
FatI CATG 9 cut(s) 351, 412, 506, 960, 1005, 1021, 1039, 1108, 1374
FauNDI CATATG 3 cut(s) 724, 1330, 1353
FbaI TGATCA 2 cut(s) 811, 1165
FblI GTMKAC 2 cut(s) 886, 1323
Fnu4HI GCNGC 4 cut(s) 6, 156, 1361, 1504
FokI GGATG 2 cut(s) 445, 938
FriOI GRGCYC 1 cut(s) 1461
Fsp4HI GCNGC 4 cut(s) 6, 156, 1361, 1504
FspAI RTGCGCAY 1 cut(s) 348
FspBI CTAG 2 cut(s) 320, 1308
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 1 cut(s) 348
GluI GCNGC 4 cut(s) 6, 156, 1361, 1504
HaeIII GGCC 2 cut(s) 29, 1155
HapII CCGG 6 cut(s) 26, 70, 105, 117, 951, 1627
HgaI GACGC 2 cut(s) 197, 1199
HhaI GCGC 1 cut(s) 349
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 9 cut(s) 355, 416, 510, 964, 1009, 1025, 1043, 1112, 1378
Hin6I GCGC 1 cut(s) 347
HinP1I GCGC 1 cut(s) 347
HincII GTYRAC 2 cut(s) 466, 730
HindII GTYRAC 2 cut(s) 466, 730
HindIII AAGCTT 3 cut(s) 244, 591, 841
HinfI GANTC 6 cut(s) 195, 323, 476, 819, 1335, 1537
HpaII CCGG 6 cut(s) 26, 70, 105, 117, 951, 1627
HphI GGTGA 3 cut(s) 412, 940, 1389
Hpy166II GTNNAC 6 cut(s) 466, 730, 806, 887, 1186, 1324
Hpy188I TCNGA 8 cut(s) 124, 145, 284, 363, 439, 826, 877, 1533
Hpy8I GTNNAC 6 cut(s) 466, 730, 806, 887, 1186, 1324
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 146, 1444
HpyCH4III ACNGT 6 cut(s) 473, 734, 803, 881, 1493, 1603
HpyCH4IV ACGT 1 cut(s) 1026
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 4 cut(s) 384, 393, 823, 1532
HpySE526I ACGT 1 cut(s) 1026
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 9 cut(s) 355, 416, 510, 964, 1009, 1025, 1043, 1112, 1378
HspAI GCGC 1 cut(s) 347
Kpn2I TCCGGA 1 cut(s) 104
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 811, 1165
Kzo9I GATC 4 cut(s) 124, 811, 1165, 1204
Lsp1109I GCAGC 3 cut(s) 167, 1347, 1490
LweI GCATC 7 cut(s) 159, 334, 874, 1041, 1266, 1347, 1439
MaeI CTAG 2 cut(s) 320, 1308
MaeII ACGT 1 cut(s) 1026
MaeIII GTNAC 4 cut(s) 683, 734, 946, 1409
MalI GATC 4 cut(s) 126, 813, 1167, 1206
MboI GATC 4 cut(s) 124, 811, 1165, 1204
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 1461
MluCI AATT 6 cut(s) 440, 543, 788, 1366, 1468, 1517
MlyI GAGTC 3 cut(s) 332, 1329, 1546
MmeI TCCRAC 3 cut(s) 40, 102, 243
Mph1103I ATGCAT 2 cut(s) 867, 980
MroI TCCGGA 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 3 cut(s) 112, 480, 1469
MseI TTAA 5 cut(s) 536, 758, 1236, 1437, 1488
MslI CAYNNNNRTG 1 cut(s) 995
MspI CCGG 6 cut(s) 26, 70, 105, 117, 951, 1627
MspR9I CCNGG 3 cut(s) 118, 600, 1500
Mva1269I GAATGC 2 cut(s) 385, 867
MvaI CCWGG 2 cut(s) 600, 1500
MwoI GCNNNNNNNGC 1 cut(s) 389
NaeI GCCGGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 2 cut(s) 506, 960
NdeI CATATG 3 cut(s) 724, 1330, 1353
NdeII GATC 4 cut(s) 124, 811, 1165, 1204
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 9 cut(s) 355, 416, 510, 964, 1009, 1025, 1043, 1112, 1378
NlaIV GGNNCC 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 946
NsbI TGCGCA 1 cut(s) 348
NsiI ATGCAT 2 cut(s) 867, 980
NspI RCATGY 1 cut(s) 1009
NspV TTCGAA 1 cut(s) 240
PagI TCATGA 1 cut(s) 1108
PciI ACATGT 1 cut(s) 1005
PctI GAATGC 2 cut(s) 385, 867
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 3 cut(s) 112, 480, 1469
PfeI GAWTC 3 cut(s) 195, 476, 819
PinAI ACCGGT 1 cut(s) 1626
PkrI GCNGC 4 cut(s) 7, 157, 1362, 1505
PleI GAGTC 3 cut(s) 331, 1329, 1545
PpsI GAGTC 3 cut(s) 331, 1329, 1545
PscI ACATGT 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 1461
Psp6I CCWGG 2 cut(s) 598, 1498
PspGI CCWGG 2 cut(s) 598, 1498
PspN4I GGNNCC 1 cut(s) 212
PspPI GGNCC 2 cut(s) 510, 1154
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 3 cut(s) 179, 664, 800
RsaNI GTAC 3 cut(s) 178, 663, 799
RseI CAYNNNNRTG 1 cut(s) 995
SacI GAGCTC 1 cut(s) 1461
SaqAI TTAA 5 cut(s) 536, 758, 1236, 1437, 1488
SatI GCNGC 4 cut(s) 6, 156, 1361, 1504
Sau3AI GATC 4 cut(s) 124, 811, 1165, 1204
Sau96I GGNCC 2 cut(s) 510, 1154
SchI GAGTC 3 cut(s) 332, 1329, 1546
ScrFI CCNGG 3 cut(s) 118, 600, 1500
SduI GDGCHC 1 cut(s) 1461
SfaNI GCATC 7 cut(s) 159, 334, 874, 1041, 1266, 1347, 1439
SfcI CTRYAG 1 cut(s) 880
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 3 cut(s) 200, 425, 708
SmoI CTYRAG 3 cut(s) 200, 425, 708
Sse9I AATT 6 cut(s) 440, 543, 788, 1366, 1468, 1517
SsiI CCGC 1 cut(s) 33
SspMI CTAG 2 cut(s) 320, 1308
SstI GAGCTC 1 cut(s) 1461
StyD4I CCNGG 3 cut(s) 116, 598, 1498
StyI CCWWGG 5 cut(s) 506, 960, 1157, 1292, 1425
TaaI ACNGT 6 cut(s) 473, 734, 803, 881, 1493, 1603
TaiI ACGT 1 cut(s) 1029
TaqI TCGA 2 cut(s) 90, 240
TasI AATT 6 cut(s) 440, 543, 788, 1366, 1468, 1517
TfiI GAWTC 3 cut(s) 195, 476, 819
Tru1I TTAA 5 cut(s) 536, 758, 1236, 1437, 1488
Tru9I TTAA 5 cut(s) 536, 758, 1236, 1437, 1488
TscAI CASTG 1 cut(s) 478
TseFI GTSAC 1 cut(s) 946
TseI GCWGC 4 cut(s) 5, 155, 1360, 1503
Tsp45I GTSAC 1 cut(s) 946
TspDTI ATGAA 5 cut(s) 539, 575, 1028, 1097, 1554
TspRI CASTG 1 cut(s) 478
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 2 cut(s) 788, 1468
XbaI TCTAGA 1 cut(s) 319
XceI RCATGY 1 cut(s) 1009
XmiI GTMKAC 2 cut(s) 886, 1323
XmnI GAANNNNTTC 3 cut(s) 112, 480, 1469
XspI CTAG 2 cut(s) 320, 1308
Zsp2I ATGCAT 2 cut(s) 867, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.