Rmu_sc0005528.1_g000003

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005528.1
OG0006101
Physical Location & Seq
Forward (+)
27365 .. 31211
3847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005528.1_g000003.1.cds

Sequence Viewer

Length: 1359 bp
atgttggatagaaagatatgtattggacactctttgctttatgtttggtatgcctcctttctagagtccaagggcaagttatacgatgcgcacatggtttatcagatgggtttttcaaggaatgctaagcctgctgagtgggtgaagaaagcacacggcttatttcttgagaggatgtctgaattagtttcttcaactcacaaggttgacgatggtgaatccattcagtttgacagccacatcaatccatggtccagttccacaatgaaagccctattacagaaaattactcctcaacttatgaaatacgatggatatcactccactaataaagcttacccaggaaaagtggcattgtcttctttgaagaactcatcaaggaacaagattattgagataggtcgtacaaaataccagattacgggttacgcaggtcaaggtggttttgctcaagtatataaagcatatgtcaactgtaaccctgatgatgttgttgcattaaagatacaaaagcctgctttcccgtgggaattctatatgtaccgtgaacttgatcaacgaatctcagacaaggaaaggtcaagctttggtttcgctcaaagaatgcatctctattctgactgtagtatacttgtctgcaactatattgctaatgggacacttcaggatgtaataaactcatttgcagtcaccggaaaatccatggaagaagtgttatgcatttattacaccatagaaatgctctacatgttagaaactctgcatgacgttggcatcattcatggagatttcaagcctgataatctgcttattcgctattctcgggacttccccacaaaaaatggatttcatgacagaaatgagcttacaaaagatggatttcgggacagaagtggcccttggcttgatcagggtctttgccttgtggactggggaagagggatagatctgcgtctctttcctaacaatatggagtttaagggagattgccgaacttctggatttcgttgtgtggagatgcaagagaataagccttggacatttcaggtagacacatatggactctgtgttgttgtccatatgatgctgcataattcttacatggagattgacaagaaaccatcacctgatggtggttacgcttatctacccaaggcatcttttaaaagatactggaaggttgagctctggaagaatttctttgtgaaactgcttaacagtaatcctggcggcaatgacaagaaattgttgcaaaatctgagggagtcctttcaggaatacatgtgctcggaccctcaccttataaagaaactaagcgatttactggcaaagcaaaggaaatcaatgtgttctgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

452

Amino Acids

52.25

Weight (kDa)

8.8

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1304
Acc16I TGCGCA 1 cut(s) 90
Acc36I ACCTGC 1 cut(s) 422
AccI GTMKAC 2 cut(s) 628, 1050
AciI CCGC 1 cut(s) 1230
AcsI RAATTY 2 cut(s) 530, 1195
AcuI CTGAAG 1 cut(s) 647
AfaI GTAC 2 cut(s) 406, 542
AfiI CCNNNNNNNGG 2 cut(s) 421, 1133
AflIII ACRYGT 2 cut(s) 747, 1281
AgsI TTSAA 4 cut(s) 117, 195, 367, 793
AjnI CCWGG 2 cut(s) 340, 1225
AjuI GAANNNNNNNTTGG 2 cut(s) 883, 915
AluBI AGCT 4 cut(s) 335, 585, 865, 1186
AluI AGCT 4 cut(s) 335, 585, 865, 1186
Alw21I GWGCWC 2 cut(s) 1188, 1289
Alw26I GTCTC 1 cut(s) 959
Ama87I CYCGRG 1 cut(s) 822
AoxI GGCC 1 cut(s) 895
ApeKI GCWGC 1 cut(s) 1087
ApoI RAATTY 2 cut(s) 530, 1195
Asp700I GAANNNNTTC 2 cut(s) 222, 1196
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 3 cut(s) 252, 896, 1291
AsuHPI GGTGA 5 cut(s) 154, 227, 682, 1116, 1289
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 2 cut(s) 252, 1291
BanII GRGCYC 1 cut(s) 1188
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 2 cut(s) 1188, 1289
BbvI GCAGC 1 cut(s) 1074
BccI CCATC 6 cut(s) 100, 206, 305, 869, 1124, 1129
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 2 cut(s) 342, 1227
BclI TGATCA 2 cut(s) 553, 907
BcoDI GTCTC 1 cut(s) 959
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 1 cut(s) 622
BfuAI ACCTGC 1 cut(s) 422
BglII AGATCT 1 cut(s) 946
BisI GCNGC 2 cut(s) 1088, 1231
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 2 cut(s) 1089, 1232
Bme1390I CCNGG 2 cut(s) 342, 1227
Bme18I GGWCC 2 cut(s) 252, 1291
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 3 cut(s) 252, 896, 1291
BmiI GGNNCC 1 cut(s) 1293
BmrFI CCNGG 2 cut(s) 342, 1227
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 6 cut(s) 76, 616, 783, 1008, 1074, 1166
BmuI ACTGGG 1 cut(s) 940
BpiI GAAGAC 1 cut(s) 351
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 188, 435
BsaBI GATNNNNATC 1 cut(s) 315
BsaJI CCNNGG 8 cut(s) 69, 248, 340, 524, 702, 899, 1034, 1152
BsaWI WCCGGW 1 cut(s) 692
Bsc4I CCNNNNNNNGG 2 cut(s) 421, 1133
Bse1I ACTGG 4 cut(s) 255, 935, 1178, 1329
Bse3DI GCAATG 1 cut(s) 1240
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 2 cut(s) 342, 1227
BseDI CCNNGG 8 cut(s) 69, 248, 340, 524, 702, 899, 1034, 1152
BseGI GGATG 2 cut(s) 180, 673
BseJI GATNNNNATC 1 cut(s) 315
BseLI CCNNNNNNNGG 2 cut(s) 421, 1133
BseMI GCAATG 1 cut(s) 1240
BseMII CTCAG 3 cut(s) 126, 579, 1250
BseNI ACTGG 4 cut(s) 255, 935, 1178, 1329
BseRI GAGGAG 1 cut(s) 282
BseXI GCAGC 1 cut(s) 1074
BshFI GGCC 1 cut(s) 897
BsiHKAI GWGCWC 2 cut(s) 1188, 1289
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 1 cut(s) 693
BslFI GGGAC 3 cut(s) 670, 839, 899
BslI CCNNNNNNNGG 2 cut(s) 421, 1133
BsmAI GTCTC 1 cut(s) 959
BsmBI CGTCTC 1 cut(s) 959
BsmFI GGGAC 3 cut(s) 670, 839, 899
BsmI GAATGC 2 cut(s) 127, 609
BsnI GGCC 1 cut(s) 897
BsoBI CYCGRG 1 cut(s) 822
Bsp1286I GDGCHC 2 cut(s) 1188, 1289
Bsp143I GATC 3 cut(s) 553, 907, 946
Bsp1720I GCTNAGC 1 cut(s) 126
Bsp19I CCATGG 2 cut(s) 248, 702
BspACI CCGC 1 cut(s) 1230
BspANI GGCC 1 cut(s) 897
BspCNI CTCAG 3 cut(s) 127, 578, 1251
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 1 cut(s) 1293
BspMI ACCTGC 1 cut(s) 422
BsrDI GCAATG 1 cut(s) 1240
BsrI ACTGG 4 cut(s) 255, 935, 1178, 1329
BssECI CCNNGG 8 cut(s) 69, 248, 340, 524, 702, 899, 1034, 1152
BssMI GATC 3 cut(s) 553, 907, 946
BssNAI GTATAC 1 cut(s) 629
BssT1I CCWWGG 6 cut(s) 69, 248, 702, 899, 1034, 1152
Bst1107I GTATAC 1 cut(s) 629
Bst2UI CCWGG 2 cut(s) 342, 1227
Bst4CI ACNGT 4 cut(s) 476, 545, 623, 1220
Bst6I CTCTTC 1 cut(s) 931
BstC8I GCNNGC 2 cut(s) 132, 516
BstDEI CTNAG 6 cut(s) 126, 135, 565, 1259, 1313, 1356
BstDSI CCRYGG 3 cut(s) 248, 524, 702
BstF5I GGATG 2 cut(s) 180, 673
BstHHI GCGC 1 cut(s) 91
BstKTI GATC 3 cut(s) 556, 910, 949
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 3 cut(s) 553, 907, 946
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 2 cut(s) 342, 1227
BstNSI RCATGY 2 cut(s) 751, 1285
BstSCI CCNGG 2 cut(s) 340, 1225
BstSFI CTRYAG 1 cut(s) 622
BstV1I GCAGC 1 cut(s) 1074
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 946
BstYI RGATCY 1 cut(s) 946
BstZ17I GTATAC 1 cut(s) 629
BsuRI GGCC 1 cut(s) 897
BtgI CCRYGG 3 cut(s) 248, 524, 702
BtsCI GGATG 2 cut(s) 180, 673
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 132, 516
CciI TCATGA 1 cut(s) 850
CfoI GCGC 1 cut(s) 91
Cfr13I GGNCC 3 cut(s) 252, 896, 1291
CseI GACGC 1 cut(s) 941
Csp6I GTAC 2 cut(s) 405, 541
CviAII CATG 9 cut(s) 94, 249, 703, 748, 764, 782, 851, 1102, 1282
CviQI GTAC 2 cut(s) 405, 541
DdeI CTNAG 6 cut(s) 126, 135, 565, 1259, 1313, 1356
DpnI GATC 3 cut(s) 555, 909, 948
DpnII GATC 3 cut(s) 553, 907, 946
DraI TTTAAA 1 cut(s) 1165
Eam1104I CTCTTC 1 cut(s) 931
EarI CTCTTC 1 cut(s) 931
Ecl136II GAGCTC 1 cut(s) 1186
Eco130I CCWWGG 6 cut(s) 69, 248, 702, 899, 1034, 1152
Eco24I GRGCYC 1 cut(s) 1188
Eco32I GATATC 1 cut(s) 317
Eco47I GGWCC 2 cut(s) 252, 1291
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 1 cut(s) 647
Eco88I CYCGRG 1 cut(s) 822
EcoICRI GAGCTC 1 cut(s) 1186
EcoRI GAATTC 1 cut(s) 530
EcoRII CCWGG 2 cut(s) 340, 1225
EcoRV GATATC 1 cut(s) 317
EcoT14I CCWWGG 6 cut(s) 69, 248, 702, 899, 1034, 1152
EcoT22I ATGCAT 2 cut(s) 609, 722
EcoT38I GRGCYC 1 cut(s) 1188
ErhI CCWWGG 6 cut(s) 69, 248, 702, 899, 1034, 1152
Esp3I CGTCTC 1 cut(s) 959
FaeI CATG 9 cut(s) 97, 252, 706, 751, 767, 785, 854, 1105, 1285
FalI AAGNNNNNCTT 4 cut(s) 883, 915, 1184, 1216
FaqI GGGAC 3 cut(s) 670, 839, 899
FatI CATG 9 cut(s) 93, 248, 702, 747, 763, 781, 850, 1101, 1281
FauNDI CATATG 3 cut(s) 466, 1057, 1080
FbaI TGATCA 2 cut(s) 553, 907
FblI GTMKAC 2 cut(s) 628, 1050
Fnu4HI GCNGC 2 cut(s) 1088, 1231
FokI GGATG 2 cut(s) 187, 680
FriOI GRGCYC 1 cut(s) 1188
Fsp4HI GCNGC 2 cut(s) 1088, 1231
FspAI RTGCGCAY 1 cut(s) 90
FspBI CTAG 1 cut(s) 62
FspI TGCGCA 1 cut(s) 90
GlaI GCGC 1 cut(s) 90
GluI GCNGC 2 cut(s) 1088, 1231
HaeIII GGCC 1 cut(s) 897
HapII CCGG 1 cut(s) 693
HgaI GACGC 1 cut(s) 941
HhaI GCGC 1 cut(s) 91
Hin1II CATG 9 cut(s) 97, 252, 706, 751, 767, 785, 854, 1105, 1285
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HincII GTYRAC 2 cut(s) 208, 472
HindII GTYRAC 2 cut(s) 208, 472
HindIII AAGCTT 2 cut(s) 333, 583
HinfI GANTC 5 cut(s) 65, 218, 561, 1062, 1265
HpaII CCGG 1 cut(s) 693
HphI GGTGA 5 cut(s) 154, 227, 682, 1116, 1289
Hpy166II GTNNAC 6 cut(s) 208, 472, 548, 629, 928, 1051
Hpy188I TCNGA 6 cut(s) 105, 181, 568, 619, 1260, 1291
Hpy188III TCNNGA 9 cut(s) 62, 167, 665, 824, 851, 884, 999, 1189, 1274
Hpy8I GTNNAC 6 cut(s) 208, 472, 548, 629, 928, 1051
HpyAV CCTTC 1 cut(s) 1171
HpyCH4III ACNGT 4 cut(s) 476, 545, 623, 1220
HpyCH4IV ACGT 1 cut(s) 768
HpyCH4V TGCA 9 cut(s) 497, 607, 639, 686, 720, 763, 1021, 1090, 1252
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 6 cut(s) 126, 135, 565, 1259, 1313, 1356
HpySE526I ACGT 1 cut(s) 768
Hsp92II CATG 9 cut(s) 97, 252, 706, 751, 767, 785, 854, 1105, 1285
HspAI GCGC 1 cut(s) 89
Ksp22I TGATCA 2 cut(s) 553, 907
Kzo9I GATC 3 cut(s) 553, 907, 946
Lsp1109I GCAGC 1 cut(s) 1074
LweI GCATC 6 cut(s) 76, 616, 783, 1008, 1074, 1166
MaeI CTAG 1 cut(s) 62
MaeII ACGT 1 cut(s) 768
MaeIII GTNAC 4 cut(s) 425, 476, 688, 1136
MalI GATC 3 cut(s) 555, 909, 948
MboI GATC 3 cut(s) 553, 907, 946
MboII GAAGA 7 cut(s) 157, 183, 351, 379, 719, 948, 1204
MflI RGATCY 1 cut(s) 946
MhlI GDGCHC 2 cut(s) 1188, 1289
MluCI AATT 6 cut(s) 182, 285, 530, 1093, 1195, 1244
MlyI GAGTC 3 cut(s) 74, 1056, 1274
MnlI CCTC 6 cut(s) 64, 165, 303, 932, 1254, 1305
Mph1103I ATGCAT 2 cut(s) 609, 722
MroXI GAANNNNTTC 2 cut(s) 222, 1196
MseI TTAA 4 cut(s) 500, 978, 1164, 1215
MslI CAYNNNNRTG 1 cut(s) 737
MspI CCGG 1 cut(s) 693
MspR9I CCNGG 2 cut(s) 342, 1227
Mva1269I GAATGC 2 cut(s) 127, 609
MvaI CCWGG 2 cut(s) 342, 1227
MwoI GCNNNNNNNGC 1 cut(s) 131
NcoI CCATGG 2 cut(s) 248, 702
NdeI CATATG 3 cut(s) 466, 1057, 1080
NdeII GATC 3 cut(s) 553, 907, 946
NlaIII CATG 9 cut(s) 97, 252, 706, 751, 767, 785, 854, 1105, 1285
NlaIV GGNNCC 1 cut(s) 1293
NmuCI GTSAC 1 cut(s) 688
NsbI TGCGCA 1 cut(s) 90
NsiI ATGCAT 2 cut(s) 609, 722
NspI RCATGY 2 cut(s) 751, 1285
PagI TCATGA 1 cut(s) 850
PciI ACATGT 2 cut(s) 747, 1281
PctI GAATGC 2 cut(s) 127, 609
PdmI GAANNNNTTC 2 cut(s) 222, 1196
PfeI GAWTC 2 cut(s) 218, 561
PkrI GCNGC 2 cut(s) 1089, 1232
PleI GAGTC 3 cut(s) 73, 1056, 1273
PpsI GAGTC 3 cut(s) 73, 1056, 1273
PscI ACATGT 2 cut(s) 747, 1281
PsiI TTATAA 1 cut(s) 1304
Psp124BI GAGCTC 1 cut(s) 1188
Psp6I CCWGG 2 cut(s) 340, 1225
PspGI CCWGG 2 cut(s) 340, 1225
PspN4I GGNNCC 1 cut(s) 1293
PspPI GGNCC 3 cut(s) 252, 896, 1291
PsuI RGATCY 1 cut(s) 946
RsaI GTAC 2 cut(s) 406, 542
RsaNI GTAC 2 cut(s) 405, 541
RseI CAYNNNNRTG 1 cut(s) 737
SacI GAGCTC 1 cut(s) 1188
SaqAI TTAA 4 cut(s) 500, 978, 1164, 1215
SatI GCNGC 2 cut(s) 1088, 1231
Sau3AI GATC 3 cut(s) 553, 907, 946
Sau96I GGNCC 3 cut(s) 252, 896, 1291
SchI GAGTC 3 cut(s) 74, 1056, 1274
ScrFI CCNGG 2 cut(s) 342, 1227
SduI GDGCHC 2 cut(s) 1188, 1289
SfaNI GCATC 6 cut(s) 76, 616, 783, 1008, 1074, 1166
SfcI CTRYAG 1 cut(s) 622
SinI GGWCC 2 cut(s) 252, 1291
SmiMI CAYNNNNRTG 1 cut(s) 737
SmlI CTYRAG 2 cut(s) 167, 450
SmoI CTYRAG 2 cut(s) 167, 450
Sse9I AATT 6 cut(s) 182, 285, 530, 1093, 1195, 1244
SsiI CCGC 1 cut(s) 1230
SspMI CTAG 1 cut(s) 62
SstI GAGCTC 1 cut(s) 1188
StyD4I CCNGG 2 cut(s) 340, 1225
StyI CCWWGG 6 cut(s) 69, 248, 702, 899, 1034, 1152
TaaI ACNGT 4 cut(s) 476, 545, 623, 1220
TaiI ACGT 1 cut(s) 771
TasI AATT 6 cut(s) 182, 285, 530, 1093, 1195, 1244
TauI GCSGC 1 cut(s) 1233
TfiI GAWTC 2 cut(s) 218, 561
Tru1I TTAA 4 cut(s) 500, 978, 1164, 1215
Tru9I TTAA 4 cut(s) 500, 978, 1164, 1215
TseFI GTSAC 1 cut(s) 688
TseI GCWGC 1 cut(s) 1087
Tsp45I GTSAC 1 cut(s) 688
TspDTI ATGAA 4 cut(s) 281, 317, 770, 839
VpaK11BI GGWCC 2 cut(s) 252, 1291
XapI RAATTY 2 cut(s) 530, 1195
XbaI TCTAGA 1 cut(s) 61
XceI RCATGY 2 cut(s) 751, 1285
XmiI GTMKAC 2 cut(s) 628, 1050
XmnI GAANNNNTTC 2 cut(s) 222, 1196
XspI CTAG 1 cut(s) 62
Zsp2I ATGCAT 2 cut(s) 609, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.