Rh2AG324200

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006101
Physical Location & Seq
Reverse (-)
46632817 .. 46637817
5001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG324200.1

Sequence Viewer

Length: 1218 bp
ATGGCTGCCGTCTTCCAACACTCGCCGGCCACCGCCATTCCTCTTCACGACCCTCTCTTGCCCTCCCTCCGGTTGATAAAGCAAGCGCTCGATGACTTCAACTCCGGAAACCCTTCCGGGTCGGATCTCACTAAGCTTCTATCCAATTGCATCGCAGCTTTCAAGGACAGTGTTCGGTACCGAGACGACACCAGATTCCTCAAGATTTGGTTCCTCTATATGGGGTTTATTGAGGATTTCGAAAGCTTGTTTATGGAAATGTTGGATAGAAAGATATGTATTGGACAGTCTTTGCTTTATGTTTGGTATGCCTCCTTTCTAGAGTCCAAGGGCAAGTTATACGATGCGCACATGGTTTATCAGATGGGTTTTTCAAGGAATGCTAAGCCTGCTGAGTGGGTGAAGAAAGCACACGGCTTATTTCTTGAGAGGATGTCTGAATTAGTTTCTTCAACTCACAAGGTTGACGACGGTGAATCCATTCAGTTTGACAGCCACATCAATCCATGGTCCAGTTCCACAATGAAAGCCCTATTAAAGAAAATTACTCCTCAACTTATGAAATACGATGGATATCACTCCACTATTAAAGCTTACCCAGGAAAAGTGGCATTGTCTTCTTTGAAGAACTCATCAAGGAACAAGATTATTGAGATAGGTCGTACAAAATACCAGATTACGGGTTACGCAGGTCAAGGTGGTTTTGCTCAAGTATATAAAGCAAATGTCAACTGTAACCCTGATGATGTTGTTGCATTAAAGATACAAAAGCCTGCTTTCCCGTGGGAATTCTATATGTACCGTGAACTTGATCAACGAATCTCAGACAAGGAAAGGTCAAGCTTTGGTTTCGCTCAAAGAATGCATCTCTATTCTGACTGTAGTATACTTGTCTGCAACTATATTGCTAATGGGACACTTCAGGATGTAATAAACTCATTTGCAGTCACCGGAAAATCCATGGAAGAGGTGTTATGCATTTATTACACCATAGAAATGCTCTACATGTTAGAAACTCTGCATGACGTTGGCATCATTCATGGAGATTTCAAGCCTGATAATCTGCTTATTCGCTATTCTCGGGACTTCCCCACAAAAAATGGATTTCATGACAGAAATGAGCTTACAAAAGATGGATTTCGGGACAGAAGTGGCCCTTGGCTTGATCAGGTCATTTGTGGTTCCAGAAATCTAGTACTTGGTTTAGTTAATTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

405

Amino Acids

46.28

Weight (kDa)

8.03

Isoelectric Point (pI)

34.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 16 - 123 1.4e-23 Mad3/BUB1 homology region 1
Pkinase PF00069 225 - 359 3.1e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 348
Acc36I ACCTGC 1 cut(s) 680
Acc65I GGTACC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 886
AccIII TCCGGA 1 cut(s) 104
AciI CCGC 1 cut(s) 33
AclWI GGATC 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 27
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 1 cut(s) 905
AfaI GTAC 4 cut(s) 179, 664, 800, 1197
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 3 cut(s) 69, 220, 679
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 6 cut(s) 100, 163, 375, 453, 625, 1051
AjnI CCWGG 1 cut(s) 598
AjuI GAANNNNNNNTTGG 2 cut(s) 1141, 1173
AluBI AGCT 6 cut(s) 136, 158, 246, 593, 843, 1123
AluI AGCT 6 cut(s) 136, 158, 246, 593, 843, 1123
Alw26I GTCTC 1 cut(s) 177
AlwI GGATC 1 cut(s) 132
Ama87I CYCGRG 1 cut(s) 1080
Aor13HI TCCGGA 1 cut(s) 104
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 2 cut(s) 27, 1153
ApeKI GCWGC 2 cut(s) 5, 155
ApoI RAATTY 1 cut(s) 788
Asp700I GAANNNNTTC 2 cut(s) 112, 480
Asp718I GGTACC 1 cut(s) 177
AspLEI GCGC 2 cut(s) 88, 349
AspS9I GGNCC 2 cut(s) 510, 1154
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 3 cut(s) 412, 485, 940
AsuII TTCGAA 1 cut(s) 240
AvaI CYCGRG 1 cut(s) 1080
AvaII GGWCC 1 cut(s) 510
BanI GGYRCC 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 4, 609
BbvI GCAGC 1 cut(s) 167
BccI CCATC 3 cut(s) 358, 563, 1127
BceAI ACGGC 1 cut(s) 430
BciT130I CCWGG 1 cut(s) 600
BclI TGATCA 2 cut(s) 811, 1165
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 177
BfaI CTAG 2 cut(s) 320, 1193
BfmI CTRYAG 1 cut(s) 880
BfoI RGCGCY 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 680
BisI GCNGC 2 cut(s) 6, 156
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 2 cut(s) 7, 157
BmcAI AGTACT 1 cut(s) 1197
Bme1390I CCNGG 2 cut(s) 118, 600
Bme18I GGWCC 1 cut(s) 510
BmeT110I CYCGRG 1 cut(s) 1080
BmgT120I GGNCC 2 cut(s) 510, 1154
BmiI GGNNCC 3 cut(s) 179, 212, 1183
BmrFI CCNGG 2 cut(s) 118, 600
BmsI GCATC 4 cut(s) 159, 334, 874, 1041
BpiI GAAGAC 2 cut(s) 4, 609
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 3 cut(s) 185, 446, 693
BpuMI CCSGG 1 cut(s) 118
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 6 cut(s) 327, 506, 598, 782, 960, 1157
BsaWI WCCGGW 3 cut(s) 69, 104, 950
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 220, 679
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 513
Bse8I GATNNNNATC 1 cut(s) 573
BseAI TCCGGA 1 cut(s) 104
BseBI CCWGG 1 cut(s) 600
BseDI CCNNGG 6 cut(s) 327, 506, 598, 782, 960, 1157
BseGI GGATG 2 cut(s) 438, 931
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 3 cut(s) 69, 220, 679
BseMII CTCAG 2 cut(s) 384, 837
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 540
BseXI GCAGC 1 cut(s) 167
BshFI GGCC 2 cut(s) 29, 1155
BshNI GGYRCC 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 1080
BsiSI CCGG 5 cut(s) 26, 70, 105, 117, 951
BslFI GGGAC 3 cut(s) 928, 1097, 1157
BslI CCNNNNNNNGG 3 cut(s) 69, 220, 679
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
BsmFI GGGAC 3 cut(s) 928, 1097, 1157
BsmI GAATGC 2 cut(s) 385, 867
BsnI GGCC 2 cut(s) 29, 1155
BsoBI CYCGRG 1 cut(s) 1080
Bsp119I TTCGAA 1 cut(s) 240
Bsp13I TCCGGA 1 cut(s) 104
Bsp143I GATC 3 cut(s) 124, 811, 1165
Bsp1720I GCTNAGC 1 cut(s) 384
Bsp19I CCATGG 2 cut(s) 506, 960
BspACI CCGC 1 cut(s) 33
BspANI GGCC 2 cut(s) 29, 1155
BspCNI CTCAG 2 cut(s) 385, 836
BspEI TCCGGA 1 cut(s) 104
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 3 cut(s) 179, 212, 1183
BspMI ACCTGC 1 cut(s) 680
BspPI GGATC 1 cut(s) 132
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 513
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 6 cut(s) 327, 506, 598, 782, 960, 1157
BssMI GATC 3 cut(s) 124, 811, 1165
BssNAI GTATAC 1 cut(s) 887
BssT1I CCWWGG 4 cut(s) 327, 506, 960, 1157
Bst1107I GTATAC 1 cut(s) 887
Bst2UI CCWGG 1 cut(s) 600
Bst4CI ACNGT 6 cut(s) 170, 288, 473, 734, 803, 881
Bst6I CTCTTC 2 cut(s) 48, 960
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 4 cut(s) 27, 84, 390, 774
BstDEI CTNAG 4 cut(s) 132, 384, 393, 823
BstDSI CCRYGG 3 cut(s) 506, 782, 960
BstF5I GGATG 2 cut(s) 438, 931
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 2 cut(s) 88, 349
BstKTI GATC 3 cut(s) 127, 814, 1168
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 3 cut(s) 124, 811, 1165
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 600
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 2 cut(s) 116, 598
BstSFI CTRYAG 1 cut(s) 880
BstV1I GCAGC 1 cut(s) 167
BstV2I GAAGAC 2 cut(s) 4, 609
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 887
BsuRI GGCC 2 cut(s) 29, 1155
BtgI CCRYGG 3 cut(s) 506, 782, 960
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 2 cut(s) 438, 931
BtsIMutI CAGTG 1 cut(s) 175
BveI ACCTGC 1 cut(s) 680
Cac8I GCNNGC 4 cut(s) 27, 84, 390, 774
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 2 cut(s) 88, 349
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 510, 1154
Csp6I GTAC 4 cut(s) 178, 663, 799, 1196
CviAII CATG 7 cut(s) 352, 507, 961, 1006, 1022, 1040, 1109
CviQI GTAC 4 cut(s) 178, 663, 799, 1196
DdeI CTNAG 4 cut(s) 132, 384, 393, 823
DpnI GATC 3 cut(s) 126, 813, 1167
DpnII GATC 3 cut(s) 124, 811, 1165
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 2 cut(s) 48, 960
EarI CTCTTC 2 cut(s) 48, 960
Eco130I CCWWGG 4 cut(s) 327, 506, 960, 1157
Eco32I GATATC 1 cut(s) 575
Eco47I GGWCC 1 cut(s) 510
Eco47III AGCGCT 1 cut(s) 87
Eco57I CTGAAG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 1080
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 598
EcoRV GATATC 1 cut(s) 575
EcoT14I CCWWGG 4 cut(s) 327, 506, 960, 1157
EcoT22I ATGCAT 2 cut(s) 867, 980
ErhI CCWWGG 4 cut(s) 327, 506, 960, 1157
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 7 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112
FalI AAGNNNNNCTT 2 cut(s) 1141, 1173
FaqI GGGAC 3 cut(s) 928, 1097, 1157
FatI CATG 7 cut(s) 351, 506, 960, 1005, 1021, 1039, 1108
FbaI TGATCA 2 cut(s) 811, 1165
FblI GTMKAC 1 cut(s) 886
Fnu4HI GCNGC 2 cut(s) 6, 156
FokI GGATG 2 cut(s) 445, 938
Fsp4HI GCNGC 2 cut(s) 6, 156
FspAI RTGCGCAY 1 cut(s) 348
FspBI CTAG 2 cut(s) 320, 1193
FspI TGCGCA 1 cut(s) 348
GlaI GCGC 2 cut(s) 87, 348
GluI GCNGC 2 cut(s) 6, 156
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 2 cut(s) 29, 1155
HapII CCGG 5 cut(s) 26, 70, 105, 117, 951
HhaI GCGC 2 cut(s) 88, 349
Hin1II CATG 7 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112
Hin6I GCGC 2 cut(s) 86, 347
HinP1I GCGC 2 cut(s) 86, 347
HincII GTYRAC 2 cut(s) 466, 730
HindII GTYRAC 2 cut(s) 466, 730
HindIII AAGCTT 4 cut(s) 134, 244, 591, 841
HinfI GANTC 4 cut(s) 195, 323, 476, 819
HpaII CCGG 5 cut(s) 26, 70, 105, 117, 951
HphI GGTGA 3 cut(s) 412, 485, 940
Hpy166II GTNNAC 4 cut(s) 466, 730, 806, 887
Hpy188I TCNGA 5 cut(s) 124, 363, 439, 826, 877
Hpy8I GTNNAC 4 cut(s) 466, 730, 806, 887
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 1 cut(s) 123
HpyCH4III ACNGT 6 cut(s) 170, 288, 473, 734, 803, 881
HpyCH4IV ACGT 1 cut(s) 1026
HpyCH4V TGCA 7 cut(s) 150, 755, 865, 897, 944, 978, 1021
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 4 cut(s) 132, 384, 393, 823
HpySE526I ACGT 1 cut(s) 1026
Hsp92II CATG 7 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112
HspAI GCGC 2 cut(s) 86, 347
Kpn2I TCCGGA 1 cut(s) 104
KpnI GGTACC 1 cut(s) 181
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
Ksp22I TGATCA 2 cut(s) 811, 1165
Kzo9I GATC 3 cut(s) 124, 811, 1165
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 4 cut(s) 159, 334, 874, 1041
MaeI CTAG 2 cut(s) 320, 1193
MaeII ACGT 1 cut(s) 1026
MaeIII GTNAC 3 cut(s) 683, 734, 946
MalI GATC 3 cut(s) 126, 813, 1167
MboI GATC 3 cut(s) 124, 811, 1165
MboII GAAGA 7 cut(s) 4, 35, 415, 441, 609, 637, 977
MfeI CAATTG 1 cut(s) 145
MflI RGATCY 1 cut(s) 124
MluCI AATT 5 cut(s) 145, 440, 543, 788, 1210
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 3 cut(s) 40, 102, 243
Mph1103I ATGCAT 2 cut(s) 867, 980
MroI TCCGGA 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 25
MroXI GAANNNNTTC 2 cut(s) 112, 480
MseI TTAA 4 cut(s) 536, 588, 758, 1209
MslI CAYNNNNRTG 1 cut(s) 995
MspI CCGG 5 cut(s) 26, 70, 105, 117, 951
MspR9I CCNGG 2 cut(s) 118, 600
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 2 cut(s) 385, 867
MvaI CCWGG 1 cut(s) 600
MwoI GCNNNNNNNGC 1 cut(s) 389
NaeI GCCGGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 2 cut(s) 506, 960
NdeII GATC 3 cut(s) 124, 811, 1165
NgoMIV GCCGGC 1 cut(s) 25
NlaIII CATG 7 cut(s) 355, 510, 964, 1009, 1025, 1043, 1112
NlaIV GGNNCC 3 cut(s) 179, 212, 1183
NmuCI GTSAC 1 cut(s) 946
NsbI TGCGCA 1 cut(s) 348
NsiI ATGCAT 2 cut(s) 867, 980
NspI RCATGY 1 cut(s) 1009
NspV TTCGAA 1 cut(s) 240
PagI TCATGA 1 cut(s) 1108
PciI ACATGT 1 cut(s) 1005
PctI GAATGC 2 cut(s) 385, 867
PdiI GCCGGC 1 cut(s) 27
PdmI GAANNNNTTC 2 cut(s) 112, 480
PfeI GAWTC 3 cut(s) 195, 476, 819
PkrI GCNGC 2 cut(s) 7, 157
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
PscI ACATGT 1 cut(s) 1005
Psp6I CCWGG 1 cut(s) 598
PspGI CCWGG 1 cut(s) 598
PspN4I GGNNCC 3 cut(s) 179, 212, 1183
PspPI GGNCC 2 cut(s) 510, 1154
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 4 cut(s) 179, 664, 800, 1197
RsaNI GTAC 4 cut(s) 178, 663, 799, 1196
RseI CAYNNNNRTG 1 cut(s) 995
SaqAI TTAA 4 cut(s) 536, 588, 758, 1209
SatI GCNGC 2 cut(s) 6, 156
Sau3AI GATC 3 cut(s) 124, 811, 1165
Sau96I GGNCC 2 cut(s) 510, 1154
ScaI AGTACT 1 cut(s) 1197
SchI GAGTC 1 cut(s) 332
ScrFI CCNGG 2 cut(s) 118, 600
SfaNI GCATC 4 cut(s) 159, 334, 874, 1041
SfcI CTRYAG 1 cut(s) 880
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 1 cut(s) 510
SmiMI CAYNNNNRTG 1 cut(s) 995
SmlI CTYRAG 3 cut(s) 200, 425, 708
SmoI CTYRAG 3 cut(s) 200, 425, 708
Sse9I AATT 5 cut(s) 145, 440, 543, 788, 1210
SsiI CCGC 1 cut(s) 33
SspMI CTAG 2 cut(s) 320, 1193
StyD4I CCNGG 2 cut(s) 116, 598
StyI CCWWGG 4 cut(s) 327, 506, 960, 1157
TaaI ACNGT 6 cut(s) 170, 288, 473, 734, 803, 881
TaiI ACGT 1 cut(s) 1029
TaqI TCGA 2 cut(s) 90, 240
TasI AATT 5 cut(s) 145, 440, 543, 788, 1210
TatI WGTACW 1 cut(s) 1195
TfiI GAWTC 3 cut(s) 195, 476, 819
Tru1I TTAA 4 cut(s) 536, 588, 758, 1209
Tru9I TTAA 4 cut(s) 536, 588, 758, 1209
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 1 cut(s) 946
TseI GCWGC 2 cut(s) 5, 155
Tsp45I GTSAC 1 cut(s) 946
TspDTI ATGAA 4 cut(s) 539, 575, 1028, 1097
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 510
XapI RAATTY 1 cut(s) 788
XbaI TCTAGA 1 cut(s) 319
XceI RCATGY 1 cut(s) 1009
XmiI GTMKAC 1 cut(s) 886
XmnI GAANNNNTTC 2 cut(s) 112, 480
XspI CTAG 2 cut(s) 320, 1193
ZrmI AGTACT 1 cut(s) 1197
Zsp2I ATGCAT 2 cut(s) 867, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.