Prupe.3G189400_v2.0.a1

checkpoint serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0006101
Physical Location & Seq
Forward (+)
20245288 .. 20250328
5041 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G189400.4

Sequence Viewer

Length: 1611 bp
ATGGCTGTCATCTTCCCTGACTCGCAACCCAGCACCCCTCTTCAGGACCCTCTCCTGCCCTGGCTCTGGTCGGTAAAGCAAGCTCTTGAGGATTGGAACTCTGGAAAAGAATCCGGGTCGGATCTCACCAAGCTTCTGTCAGATTGCATCGCTGCATTCAAGGACAATGATCAGTACCGAGACGACATCAGATTCCTCAAGATTTGGTTCTTTTATATGGATTTCAGTGAAGATTTTGGAAGCGTTTTTCAGAAAATGTGGGAGAGTAAGATTTGTGTTGGAAACGCTTTGCTTTATATTTGGTATGCGTCCTTTCTCGAATCCAAAGGAAAATTATACGATGCGAGCATGGTTTATCAGATGGGTATTTCGAGGAATGCTAAACCAGTTGAGTGGGTGAAGAAGGCACATGCCTTGTTTCTTGAAAGGATGTCTGAAATTGTTTCTTCAGCTCAGACTATTGGCAATGATGAATCGATTACATTTGGGAGCCTCACTAACCCATGGTCCAGCTCCACTATGAAAGTCCTATTAAAGAAAATTAATCATGAACTTATGAAATATGATGGATACCAGTGTACTACTAAACCTTATTCAGGAAAAGTGGCGTTGTCTTCTCTGAAGAACTCGTCAAGGAACAAGACAATTGAGATAGGTCGTACAAAGTACCAGATCATAGGTTGTGCAGGCCAGGGTGGTTTTGCTCAAGTGTATAAAGCATATGTCAGCTGTAATCCTGATGATGTTGTTGCATTGAAGATACAAAAGCCTGCTTTCCCTTGGGAGTTCTATATGTATCGTCAGCTGAATCAACGGATCTCAGACAAGGAAAGGTCAAGCTTTGGTGTCGCTCATAGAATGCATCTCTATTCTGACTGTAGCATACTCGTCTGCGATTATCTTGCTAATGGAACACTTCAGGATGCAATAAACTCATTTGCCGTCATTGGCAAGTCCATGGAAGAAGTATTATGCATTTATTACACCATAGAAATGCTCTATATGCTAGAAACTCTGCATGGTGTCGGCATCATTCATGGAGATTTCAAGCCTGATAATTTGCTTATTCGTTATGCTAGGGATGGCATTACAAAAGCTGGATCTCGCTACGGAAATGACCTTACAAAAGATGGATTTCGTGACAGAAGTGGTCCTTGGCATGATCAGGGTCTTTGCCTTGTCGATTGGGGAAGAGGGATAGATATGCATCTCTTTCCTGACAATATGGAGTTTAAGGGAGATTGCCGGACTTCTGGATTTCGTTGTGTAGAGATGCAAGAGAATAGGCCATGGACATTTCAGGTAGACACATATGGCCTCTGTGTTGTTGTCCATATGATGCTGCATAATTCTTACATGGAGATTGAAAAGAAACTGTCACCTGAAGGGGGTTACATTTATCAACCCAAGTCATCTTTTAAAAGATATTGGAATGGTGAACTTTGGAGGAATTTATTTAAGAAACTGCTTAACAGTAGCCCCGGCGGTAATGACAAGAAGTTGTTGCAGGATTTGAGGGAGACCTTCCAGGATTACATGTGCTCAGACCCTCAGCTTATAAAGAAACTAAGCGAATTACTGGTTAAGCAAAGGGCCTCATTGTGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0000940 GO:0000942 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007063 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009966 GO:0009967 GO:0009987 GO:0010564 GO:0010639 GO:0010646 GO:0010647 GO:0010941 GO:0010942 GO:0010948 GO:0010965 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0023051 GO:0023056 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1902531 GO:1902533 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001233 GO:2001235 GO:2001242 GO:2001244 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I Pkinase

Protein Analysis

537

Amino Acids

61.56

Weight (kDa)

7.81

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1559
AccI GTMKAC 1 cut(s) 1305
AciI CCGC 1 cut(s) 1485
AclWI GGATC 3 cut(s) 129, 824, 1108
AcsI RAATTY 1 cut(s) 1450
AcuI CTGAAG 5 cut(s) 26, 432, 641, 902, 1404
AfaI GTAC 4 cut(s) 176, 580, 661, 668
AfiI CCNNNNNNNGG 4 cut(s) 43, 66, 596, 1388
AflIII ACRYGT 1 cut(s) 1536
AgsI TTSAA 5 cut(s) 160, 425, 757, 1048, 1367
AjnI CCWGG 3 cut(s) 59, 690, 1527
AjuI GAANNNNNNNTTGG 2 cut(s) 1138, 1170
AluBI AGCT 9 cut(s) 83, 133, 452, 513, 729, 805, 840, 1097, 1555
AluI AGCT 9 cut(s) 83, 133, 452, 513, 729, 805, 840, 1097, 1555
Alw21I GWGCWC 1 cut(s) 1544
Alw26I GTCTC 2 cut(s) 174, 1514
AlwI GGATC 3 cut(s) 129, 824, 1108
AoxI GGCC 4 cut(s) 688, 1286, 1315, 1593
ApeKI GCWGC 2 cut(s) 152, 1342
ApoI RAATTY 1 cut(s) 1450
AseI ATTAAT 1 cut(s) 543
AspS9I GGNCC 4 cut(s) 46, 507, 1151, 1593
AsuC2I CCSGG 2 cut(s) 115, 1482
AsuHPI GGTGA 4 cut(s) 118, 409, 1371, 1448
AvaII GGWCC 3 cut(s) 46, 507, 1151
BaeI ACNNNNGTAYC 2 cut(s) 562, 595
BbsI GAAGAC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 1544
BbvCI CCTCAGC 1 cut(s) 1551
BbvI GCAGC 2 cut(s) 139, 1329
BccI CCATC 4 cut(s) 355, 560, 1076, 1124
BceAI ACGGC 1 cut(s) 926
BcgI CGANNNNNNTGC 2 cut(s) 884, 918
BciT130I CCWGG 3 cut(s) 61, 692, 1529
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 2 cut(s) 169, 1162
BcnI CCSGG 2 cut(s) 115, 1482
BcoDI GTCTC 2 cut(s) 174, 1514
BfaI CTAG 2 cut(s) 1007, 1077
BfmI CTRYAG 1 cut(s) 877
BfuI GTATCC 1 cut(s) 563
BisI GCNGC 2 cut(s) 153, 1343
BlsI GCNGC 2 cut(s) 154, 1344
Bme1390I CCNGG 5 cut(s) 61, 115, 692, 1482, 1529
Bme18I GGWCC 3 cut(s) 46, 507, 1151
BmgT120I GGNCC 4 cut(s) 46, 507, 1151, 1593
BmiI GGNNCC 2 cut(s) 48, 491
BmrFI CCNGG 5 cut(s) 61, 115, 692, 1482, 1529
BmsI GCATC 8 cut(s) 156, 331, 871, 913, 1038, 1216, 1263, 1329
BpiI GAAGAC 1 cut(s) 606
Bpu10I CCTNAGC 1 cut(s) 1551
BpuEI CTTGAG 3 cut(s) 107, 182, 690
BpuMI CCSGG 2 cut(s) 115, 1482
Bsa29I ATCGAT 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 1206
BsaI GGTCTC 1 cut(s) 1514
BsaJI CCNNGG 8 cut(s) 59, 503, 691, 779, 957, 1154, 1289, 1480
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 66, 596, 1388
Bse1I ACTGG 3 cut(s) 386, 574, 1584
Bse3DI GCAATG 1 cut(s) 472
Bse8I GATNNNNATC 1 cut(s) 1206
BseBI CCWGG 3 cut(s) 61, 692, 1529
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 8 cut(s) 59, 503, 691, 779, 957, 1154, 1289, 1480
BseGI GGATG 3 cut(s) 435, 928, 1087
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 4 cut(s) 43, 66, 596, 1388
BseMI GCAATG 1 cut(s) 472
BseMII CTCAG 4 cut(s) 467, 834, 1557, 1565
BseNI ACTGG 3 cut(s) 386, 574, 1584
BseXI GCAGC 2 cut(s) 139, 1329
BseYI CCCAGC 1 cut(s) 29
BsgI GTGCAG 1 cut(s) 705
BshFI GGCC 4 cut(s) 690, 1288, 1317, 1595
BshVI ATCGAT 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 1544
BsiSI CCGG 3 cut(s) 114, 1246, 1482
BslI CCNNNNNNNGG 4 cut(s) 43, 66, 596, 1388
BsmAI GTCTC 2 cut(s) 174, 1514
BsmBI CGTCTC 1 cut(s) 174
BsmI GAATGC 3 cut(s) 155, 382, 864
BsnI GGCC 4 cut(s) 690, 1288, 1317, 1595
Bso31I GGTCTC 1 cut(s) 1514
Bsp1286I GDGCHC 1 cut(s) 1544
Bsp143I GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
Bsp19I CCATGG 3 cut(s) 503, 957, 1289
BspACI CCGC 1 cut(s) 1485
BspANI GGCC 4 cut(s) 690, 1288, 1317, 1595
BspCNI CTCAG 4 cut(s) 466, 833, 1556, 1564
BspDI ATCGAT 1 cut(s) 476
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 2 cut(s) 48, 491
BspPI GGATC 3 cut(s) 129, 824, 1108
BspTNI GGTCTC 1 cut(s) 1514
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 3 cut(s) 386, 574, 1584
BssECI CCNNGG 8 cut(s) 59, 503, 691, 779, 957, 1154, 1289, 1480
BssMI GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
BssT1I CCWWGG 5 cut(s) 503, 779, 957, 1154, 1289
Bst2UI CCWGG 3 cut(s) 61, 692, 1529
Bst4CI ACNGT 3 cut(s) 878, 1377, 1475
Bst6I CTCTTC 2 cut(s) 45, 1186
BstC8I GCNNGC 4 cut(s) 81, 346, 688, 771
BstDEI CTNAG 6 cut(s) 453, 820, 1543, 1551, 1568, 1608
BstDSI CCRYGG 3 cut(s) 503, 957, 1289
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 3 cut(s) 435, 928, 1087
BstKTI GATC 6 cut(s) 124, 172, 675, 819, 1103, 1165
BstMAI GTCTC 2 cut(s) 174, 1514
BstMBI GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
BstMWI GCNNNNNNNGC 1 cut(s) 1003
BstNI CCWGG 3 cut(s) 61, 692, 1529
BstNSI RCATGY 2 cut(s) 413, 1540
BstSCI CCNGG 5 cut(s) 59, 113, 690, 1480, 1527
BstSFI CTRYAG 1 cut(s) 877
BstV1I GCAGC 2 cut(s) 139, 1329
BstV2I GAAGAC 1 cut(s) 606
BstX2I RGATCY 3 cut(s) 121, 816, 1100
BstXI CCANNNNNNTGG 1 cut(s) 393
BstYI RGATCY 3 cut(s) 121, 816, 1100
Bsu15I ATCGAT 1 cut(s) 476
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 4 cut(s) 690, 1288, 1317, 1595
BsuTUI ATCGAT 1 cut(s) 476
BtgI CCRYGG 3 cut(s) 503, 957, 1289
BtgZI GCGATG 1 cut(s) 133
BtsCI GGATG 3 cut(s) 435, 928, 1087
BtsIMutI CAGTG 2 cut(s) 232, 581
Cac8I GCNNGC 4 cut(s) 81, 346, 688, 771
CciI TCATGA 1 cut(s) 547
Cfr13I GGNCC 4 cut(s) 46, 507, 1151, 1593
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 1 cut(s) 297
Csp6I GTAC 4 cut(s) 175, 579, 660, 667
CviQI GTAC 4 cut(s) 175, 579, 660, 667
DdeI CTNAG 6 cut(s) 453, 820, 1543, 1551, 1568, 1608
DpnI GATC 6 cut(s) 123, 171, 674, 818, 1102, 1164
DpnII GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
DraI TTTAAA 1 cut(s) 1420
Eam1104I CTCTTC 2 cut(s) 45, 1186
EarI CTCTTC 2 cut(s) 45, 1186
Eco130I CCWWGG 5 cut(s) 503, 779, 957, 1154, 1289
Eco31I GGTCTC 1 cut(s) 1514
Eco47I GGWCC 3 cut(s) 46, 507, 1151
Eco57I CTGAAG 5 cut(s) 26, 432, 641, 902, 1404
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO109I RGGNCCY 2 cut(s) 46, 1593
EcoRII CCWGG 3 cut(s) 59, 690, 1527
EcoT14I CCWWGG 5 cut(s) 503, 779, 957, 1154, 1289
EcoT22I ATGCAT 3 cut(s) 864, 977, 1209
ErhI CCWWGG 5 cut(s) 503, 779, 957, 1154, 1289
Esp3I CGTCTC 1 cut(s) 174
FalI AAGNNNNNCTT 2 cut(s) 1138, 1170
FauNDI CATATG 3 cut(s) 721, 1312, 1335
FbaI TGATCA 2 cut(s) 169, 1162
FblI GTMKAC 1 cut(s) 1305
Fnu4HI GCNGC 2 cut(s) 153, 1343
FokI GGATG 3 cut(s) 442, 935, 1094
Fsp4HI GCNGC 2 cut(s) 153, 1343
FspBI CTAG 2 cut(s) 1007, 1077
GluI GCNGC 2 cut(s) 153, 1343
GsaI CCCAGC 1 cut(s) 33
HaeIII GGCC 4 cut(s) 690, 1288, 1317, 1595
HapII CCGG 3 cut(s) 114, 1246, 1482
HgaI GACGC 1 cut(s) 297
HindIII AAGCTT 2 cut(s) 131, 838
HinfI GANTC 6 cut(s) 20, 110, 192, 320, 473, 808
HpaII CCGG 3 cut(s) 114, 1246, 1482
HphI GGTGA 4 cut(s) 118, 409, 1371, 1448
Hpy166II GTNNAC 3 cut(s) 579, 1306, 1439
Hpy8I GTNNAC 3 cut(s) 579, 1306, 1439
HpyAV CCTTC 3 cut(s) 397, 1379, 1534
HpyCH4III ACNGT 3 cut(s) 878, 1377, 1475
HpyF10VI GCNNNNNNNGC 1 cut(s) 1003
HpyF3I CTNAG 6 cut(s) 453, 820, 1543, 1551, 1568, 1608
Ksp22I TGATCA 2 cut(s) 169, 1162
Kzo9I GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
LmnI GCTCC 2 cut(s) 489, 518
Lsp1109I GCAGC 2 cut(s) 139, 1329
LweI GCATC 8 cut(s) 156, 331, 871, 913, 1038, 1216, 1263, 1329
MaeI CTAG 2 cut(s) 1007, 1077
MaeIII GTNAC 3 cut(s) 1139, 1377, 1391
MalI GATC 6 cut(s) 123, 171, 674, 818, 1102, 1164
MboI GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
MfeI CAATTG 1 cut(s) 645
MflI RGATCY 3 cut(s) 121, 816, 1100
MhlI GDGCHC 1 cut(s) 1544
MluCI AATT 8 cut(s) 332, 438, 540, 645, 1057, 1348, 1450, 1574
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 99, 259
Mph1103I ATGCAT 3 cut(s) 864, 977, 1209
MseI TTAA 7 cut(s) 533, 543, 1233, 1419, 1458, 1470, 1584
MslI CAYNNNNRTG 1 cut(s) 992
MspA1I CMGCKG 2 cut(s) 729, 805
MspI CCGG 3 cut(s) 114, 1246, 1482
MspR9I CCNGG 5 cut(s) 61, 115, 692, 1482, 1529
MunI CAATTG 1 cut(s) 645
Mva1269I GAATGC 3 cut(s) 155, 382, 864
MvaI CCWGG 3 cut(s) 61, 692, 1529
MwoI GCNNNNNNNGC 1 cut(s) 1003
NciI CCSGG 2 cut(s) 115, 1482
NcoI CCATGG 3 cut(s) 503, 957, 1289
NdeI CATATG 3 cut(s) 721, 1312, 1335
NdeII GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
NlaIV GGNNCC 2 cut(s) 48, 491
NmuCI GTSAC 2 cut(s) 1139, 1377
NsiI ATGCAT 3 cut(s) 864, 977, 1209
NspI RCATGY 2 cut(s) 413, 1540
PagI TCATGA 1 cut(s) 547
PciI ACATGT 1 cut(s) 1536
PctI GAATGC 3 cut(s) 155, 382, 864
PfeI GAWTC 5 cut(s) 110, 192, 320, 473, 808
PfoI TCCNGGA 1 cut(s) 1527
PkrI GCNGC 2 cut(s) 154, 1344
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
PpuMI RGGWCCY 1 cut(s) 46
PscI ACATGT 1 cut(s) 1536
PshBI ATTAAT 1 cut(s) 543
PsiI TTATAA 1 cut(s) 1559
Psp5II RGGWCCY 1 cut(s) 46
Psp6I CCWGG 3 cut(s) 59, 690, 1527
PspFI CCCAGC 1 cut(s) 29
PspGI CCWGG 3 cut(s) 59, 690, 1527
PspN4I GGNNCC 2 cut(s) 48, 491
PspPI GGNCC 4 cut(s) 46, 507, 1151, 1593
PspPPI RGGWCCY 1 cut(s) 46
PsuI RGATCY 3 cut(s) 121, 816, 1100
PvuII CAGCTG 2 cut(s) 729, 805
RsaI GTAC 4 cut(s) 176, 580, 661, 668
RsaNI GTAC 4 cut(s) 175, 579, 660, 667
RseI CAYNNNNRTG 1 cut(s) 992
SaqAI TTAA 7 cut(s) 533, 543, 1233, 1419, 1458, 1470, 1584
SatI GCNGC 2 cut(s) 153, 1343
Sau3AI GATC 6 cut(s) 121, 169, 672, 816, 1100, 1162
Sau96I GGNCC 4 cut(s) 46, 507, 1151, 1593
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 5 cut(s) 61, 115, 692, 1482, 1529
SduI GDGCHC 1 cut(s) 1544
SfaNI GCATC 8 cut(s) 156, 331, 871, 913, 1038, 1216, 1263, 1329
SfcI CTRYAG 1 cut(s) 877
SinI GGWCC 3 cut(s) 46, 507, 1151
SmiMI CAYNNNNRTG 1 cut(s) 992
SmlI CTYRAG 3 cut(s) 86, 197, 705
SmoI CTYRAG 3 cut(s) 86, 197, 705
Sse9I AATT 8 cut(s) 332, 438, 540, 645, 1057, 1348, 1450, 1574
SsiI CCGC 1 cut(s) 1485
SspMI CTAG 2 cut(s) 1007, 1077
StyD4I CCNGG 5 cut(s) 59, 113, 690, 1480, 1527
StyI CCWWGG 5 cut(s) 503, 779, 957, 1154, 1289
TaaI ACNGT 3 cut(s) 878, 1377, 1475
TaqI TCGA 4 cut(s) 318, 371, 476, 1182
TasI AATT 8 cut(s) 332, 438, 540, 645, 1057, 1348, 1450, 1574
TatI WGTACW 1 cut(s) 578
TfiI GAWTC 5 cut(s) 110, 192, 320, 473, 808
Tru1I TTAA 7 cut(s) 533, 543, 1233, 1419, 1458, 1470, 1584
Tru9I TTAA 7 cut(s) 533, 543, 1233, 1419, 1458, 1470, 1584
TscAI CASTG 2 cut(s) 232, 581
TseFI GTSAC 2 cut(s) 1139, 1377
TseI GCWGC 2 cut(s) 152, 1342
Tsp45I GTSAC 2 cut(s) 1139, 1377
TspDTI ATGAA 5 cut(s) 486, 536, 564, 572, 1025
TspGWI ACGGA 2 cut(s) 829, 1125
TspRI CASTG 2 cut(s) 232, 581
VpaK11BI GGWCC 3 cut(s) 46, 507, 1151
VspI ATTAAT 1 cut(s) 543
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 1 cut(s) 1450
XceI RCATGY 2 cut(s) 413, 1540
XmiI GTMKAC 1 cut(s) 1305
XspI CTAG 2 cut(s) 1007, 1077
Zsp2I ATGCAT 3 cut(s) 864, 977, 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.