FvH4_6g27020

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011944
Physical Location & Seq
Forward (+)
20668624 .. 20670116
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27020.t1

Sequence Viewer

Length: 525 bp
ATGCGCACAAGCTTCCTTCTCTCTCTTCTCCTCCTCCTCTCGCTCGCCGCCCTATCTACCGCCCAGAACAGCGTAGTCACGACGGAGCTCTGGTGCGTGGCCAAGAACAACGCCGAGGACTCTGCCCTCCAGTCCGCTCTTGACTGGGCCTGCGGGGCCGGCGGCGCCAACTGCAGCCCAATCCAGCCAGGCGCACCCTGCTACGACGCTTCCGACATCCAGAACACTGCCTCCTATGCCTTCAACGACTACTTCCTCAAAAACGGCATGACCGATGACAGCTGCAACTTCGATAACACTGCTGCTCTGACATCTTTAAATCCCAGTTTTGGTAATTGCAAGATGCCATCCAGCTTGTCTGGGAGCAATAGAAGTTCGAGTTCATCAACAGCATCATATGGGTTGGGACCTAGTCAAGATCTGAATGGCAGCAATAAAGTTTCTCATCAACTGGTTTTGCTGCCCTTGATTACCAGTCTTTTGCTCATTGCCTCCTATAATACATGGGTAATCTGTCTACCTTGA

Protein Analysis

175

Amino Acids

18.26

Weight (kDa)

4.39

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 30 - 101 1.7e-19 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58100 AT3G58100
fragaria_vesca FvH4_6g27020 FvH4_6g27020 FvH4_6g27020
malus_domestica MD09G1240800.v1.1 MD17G1233700.v1.1
prunus_persica Prupe.3G125100_v2.0.a1
pyrus_communis pycom09g15960 pycom17g23680
rosa_chinensis RchiOBHm_Chr2g0128881
rosa_laevigata RLG00000019037
rosa_multiflora Rmu_sc0001643.1_g000031
rosa_roxburghii Rroxscaffold_2G00115140
rosa_rugosa Rorug02G0282100
rosa_samantha Rh2AG334500 Rh2CG321500 Rh2DG359200
rosa_wichuraiana Rw2G027070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 164
AccBSI CCGCTC 1 cut(s) 137
AccI GTMKAC 1 cut(s) 517
AciI CCGC 5 cut(s) 48, 60, 135, 153, 162
AcoI YGGCCR 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 165
AfiI CCNNNNNNNGG 1 cut(s) 329
AgsI TTSAA 1 cut(s) 244
AjnI CCWGG 1 cut(s) 187
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 4 cut(s) 12, 88, 282, 354
AluI AGCT 4 cut(s) 12, 88, 282, 354
Alw21I GWGCWC 1 cut(s) 90
AoxI GGCC 3 cut(s) 99, 147, 156
ApeKI GCWGC 5 cut(s) 174, 282, 302, 429, 460
AspLEI GCGC 3 cut(s) 6, 167, 194
AspS9I GGNCC 3 cut(s) 147, 156, 407
AvaII GGWCC 1 cut(s) 407
BalI TGGCCA 1 cut(s) 101
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 90
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 5 cut(s) 186, 269, 289, 441, 447
BccI CCATC 1 cut(s) 355
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 4 cut(s) 104, 138, 281, 315
BciT130I CCWGG 1 cut(s) 189
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 172
BfoI RGCGCY 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 155
BglII AGATCT 1 cut(s) 418
BisI GCNGC 7 cut(s) 48, 163, 175, 283, 303, 430, 461
BlsI GCNGC 7 cut(s) 49, 164, 176, 284, 304, 431, 462
Bme1390I CCNGG 1 cut(s) 189
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 147, 156, 407
BmiI GGNNCC 3 cut(s) 157, 166, 408
BmrFI CCNGG 1 cut(s) 189
BmrI ACTGGG 2 cut(s) 154, 318
BmsI GCATC 2 cut(s) 333, 401
BmuI ACTGGG 2 cut(s) 154, 318
BpmI CTGGAG 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 114
BsaXI ACNNNNNCTCC 4 cut(s) 77, 107, 215, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 329
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 5 cut(s) 130, 149, 324, 456, 474
Bse3DI GCAATG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 2 cut(s) 216, 347
BseLI CCNNNNNNNGG 1 cut(s) 329
BseMI GCAATG 1 cut(s) 486
BseNI ACTGG 5 cut(s) 130, 149, 324, 456, 474
BseRI GAGGAG 3 cut(s) 20, 23, 26
BseXI GCAGC 5 cut(s) 186, 269, 289, 441, 447
BshFI GGCC 3 cut(s) 101, 149, 158
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 420
BsnI GGCC 3 cut(s) 101, 149, 158
Bsp1286I GDGCHC 1 cut(s) 90
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 5 cut(s) 48, 60, 135, 153, 162
BspANI GGCC 3 cut(s) 101, 149, 158
BspLI GGNNCC 3 cut(s) 157, 166, 408
BspMAI CTGCAG 1 cut(s) 176
BspT107I GGYRCC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 486
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 5 cut(s) 130, 149, 324, 456, 474
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 189
Bst6I CTCTTC 1 cut(s) 30
BstACI GRCGYC 1 cut(s) 165
BstC8I GCNNGC 3 cut(s) 45, 151, 160
BstF5I GGATG 2 cut(s) 216, 347
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 6, 167, 194
BstKTI GATC 1 cut(s) 421
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 6 cut(s) 155, 159, 164, 171, 198, 236
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 172
BstV1I GCAGC 5 cut(s) 186, 269, 289, 441, 447
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 3 cut(s) 101, 149, 158
BtsCI GGATG 2 cut(s) 216, 347
BtsI GCAGTG 2 cut(s) 225, 297
BtsIMutI CAGTG 2 cut(s) 225, 297
Cac8I GCNNGC 3 cut(s) 45, 151, 160
CfoI GCGC 3 cut(s) 6, 167, 194
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 3 cut(s) 147, 156, 407
CseI GACGC 1 cut(s) 215
CviAII CATG 2 cut(s) 268, 504
CviJI RGCY 9 cut(s) 12, 88, 101, 149, 158, 177, 187, 282, 354
CviKI_1 RGCY 9 cut(s) 12, 88, 101, 149, 158, 177, 187, 282, 354
DinI GGCGCC 1 cut(s) 166
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
DraI TTTAAA 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 1 cut(s) 30
EarI CTCTTC 1 cut(s) 30
Ecl136II GAGCTC 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 407
Eco53kI GAGCTC 1 cut(s) 88
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 187
EcoT38I GRGCYC 1 cut(s) 90
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
FaeI CATG 2 cut(s) 271, 507
FaiI YATR 6 cut(s) 237, 269, 397, 399, 498, 505
FaqI GGGAC 1 cut(s) 420
FatI CATG 2 cut(s) 267, 503
FauI CCCGC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 397
FblI GTMKAC 1 cut(s) 517
Fnu4HI GCNGC 7 cut(s) 48, 163, 175, 283, 303, 430, 461
FokI GGATG 2 cut(s) 203, 334
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 7 cut(s) 48, 163, 175, 283, 303, 430, 461
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 1 cut(s) 411
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 3 cut(s) 5, 166, 193
GluI GCNGC 7 cut(s) 48, 163, 175, 283, 303, 430, 461
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 3 cut(s) 101, 149, 158
HapII CCGG 1 cut(s) 159
HgaI GACGC 1 cut(s) 215
HhaI GCGC 3 cut(s) 6, 167, 194
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 2 cut(s) 271, 507
Hin6I GCGC 3 cut(s) 4, 165, 192
HinP1I GCGC 3 cut(s) 4, 165, 192
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 1 cut(s) 119
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 518
Hpy188I TCNGA 3 cut(s) 214, 309, 423
Hpy188III TCNNGA 4 cut(s) 79, 140, 220, 416
Hpy8I GTNNAC 1 cut(s) 518
Hpy99I CGWCG 2 cut(s) 85, 209
HpyAV CCTTC 2 cut(s) 26, 250
HpyCH4V TGCA 3 cut(s) 174, 285, 339
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 159, 164, 171, 198, 236
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 2 cut(s) 271, 507
HspAI GCGC 3 cut(s) 4, 165, 192
KasI GGCGCC 1 cut(s) 164
KroI GCCGGC 1 cut(s) 158
KroNI GCCGGC 1 cut(s) 160
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 2 cut(s) 85, 363
Lsp1109I GCAGC 5 cut(s) 186, 269, 289, 441, 447
LweI GCATC 2 cut(s) 333, 401
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 1 cut(s) 76
MalI GATC 1 cut(s) 420
MbiI CCGCTC 1 cut(s) 137
MboI GATC 1 cut(s) 418
MboII GAAGA 1 cut(s) 17
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 1 cut(s) 90
MlsI TGGCCA 1 cut(s) 101
MluCI AATT 1 cut(s) 334
MluNI TGGCCA 1 cut(s) 101
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 113
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 8 cut(s) 41, 44, 47, 109, 137, 241, 266, 502
Mox20I TGGCCA 1 cut(s) 101
MroNI GCCGGC 1 cut(s) 158
MscI TGGCCA 1 cut(s) 101
MseI TTAA 1 cut(s) 317
Msp20I TGGCCA 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 6 cut(s) 155, 159, 164, 171, 198, 236
NaeI GCCGGC 1 cut(s) 160
NarI GGCGCC 1 cut(s) 165
NdeI CATATG 1 cut(s) 397
NdeII GATC 1 cut(s) 418
NgoMIV GCCGGC 1 cut(s) 158
NlaIII CATG 2 cut(s) 271, 507
NlaIV GGNNCC 3 cut(s) 157, 166, 408
NmeAIII GCCGAG 1 cut(s) 139
NmuCI GTSAC 1 cut(s) 76
NsbI TGCGCA 1 cut(s) 5
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 270
PdiI GCCGGC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 411
PkrI GCNGC 7 cut(s) 49, 164, 176, 284, 304, 431, 462
PleI GAGTC 1 cut(s) 113
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 113
PpuMI RGGWCCY 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 90
Psp5II RGGWCCY 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 157, 166, 408
PspPI GGNCC 3 cut(s) 147, 156, 407
PspPPI RGGWCCY 1 cut(s) 407
PstI CTGCAG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 418
PsyI GACNNNGTC 1 cut(s) 411
PvuII CAGCTG 1 cut(s) 282
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 7 cut(s) 48, 163, 175, 283, 303, 430, 461
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 3 cut(s) 147, 156, 407
SchI GAGTC 1 cut(s) 113
ScrFI CCNGG 1 cut(s) 189
SduI GDGCHC 1 cut(s) 90
SetI ASST 6 cut(s) 14, 90, 284, 356, 412, 523
SfaNI GCATC 2 cut(s) 333, 401
SfcI CTRYAG 1 cut(s) 172
SfiI GGCCNNNNNGGCC 1 cut(s) 155
SfoI GGCGCC 1 cut(s) 166
SinI GGWCC 1 cut(s) 407
Sse9I AATT 1 cut(s) 334
SsiI CCGC 5 cut(s) 48, 60, 135, 153, 162
SspDI GGCGCC 1 cut(s) 164
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 187
TaqI TCGA 2 cut(s) 291, 377
TaqII GACCGA 1 cut(s) 287
TasI AATT 1 cut(s) 334
TauI GCSGC 2 cut(s) 50, 165
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 2 cut(s) 232, 304
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 5 cut(s) 174, 282, 302, 429, 460
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 2 cut(s) 232, 304
Tth111I GACNNNGTC 1 cut(s) 411
VpaK11BI GGWCC 1 cut(s) 407
XmiI GTMKAC 1 cut(s) 517
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.