Rroxscaffold_2G00115140

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011944
Physical Location & Seq
Reverse (-)
41381305 .. 41383308
2004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115140.1

Sequence Viewer

Length: 522 bp
ATGTACACAAGCTTCCTTCTTCTCTCTCTCCTCCTCTCACTCGCCGCCCTCTCTACCGCCCAAAACAGCGTTGTCACGATGGAGCTGTGGTGCGTGGCCAAGAACAACGCCGACGACTCTGCCCTACAGTCCGCTCTCGACTGGGCCTGCGGTGCCGGCGGCGCCAACTGCAGCCCTATCCAGCAAGGTGGGCCCTGCTACGACCCTTCTGACATCCAGAACACCGCGTCGTACGCCTTCAACGACTACTTTCTCAAACACGGTATGACCGATGACAGCTGCAACTTCGATAACACTGCCGCAGTCACCTCTTTGAATCCCAGTTTTGGTAATTGCAAGTTCCCATCCAGCTTGTCAGGGAGCAATAGAAGTTCGAGTTCATCGACAGCATCATATGGGTTGGGGCCTAGTCAAGATCTGAATGGCAGCAATAAAATTTCGCAGCAACTGGTTTTGCTGCCCTTGATTACCAGTCTTTTGCTCATTGCCTCCTACAATACATGGGTCATCTGTTTATCTTGA

Protein Analysis

173

Amino Acids

18.23

Weight (kDa)

4.19

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 29 - 100 7.4e-21 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58100 AT3G58100
fragaria_vesca FvH4_6g27020 FvH4_6g27020 FvH4_6g27020
malus_domestica MD09G1240800.v1.1 MD17G1233700.v1.1
prunus_persica Prupe.3G125100_v2.0.a1
pyrus_communis pycom09g15960 pycom17g23680
rosa_chinensis RchiOBHm_Chr2g0128881
rosa_laevigata RLG00000019037
rosa_multiflora Rmu_sc0001643.1_g000031
rosa_roxburghii Rroxscaffold_2G00115140
rosa_rugosa Rorug02G0282100
rosa_samantha Rh2AG334500 Rh2CG321500 Rh2DG359200
rosa_wichuraiana Rw2G027070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 152, 161
AccBSI CCGCTC 1 cut(s) 134
AccII CGCG 1 cut(s) 227
AciI CCGC 7 cut(s) 45, 57, 132, 150, 159, 225, 300
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 2 cut(s) 5, 233
AfiI CCNNNNNNNGG 1 cut(s) 326
AgsI TTSAA 2 cut(s) 241, 316
AluBI AGCT 4 cut(s) 12, 85, 279, 351
AluI AGCT 4 cut(s) 12, 85, 279, 351
AlwNI CAGNNNCTG 1 cut(s) 448
AoxI GGCC 4 cut(s) 96, 144, 191, 404
ApaI GGGCCC 1 cut(s) 195
ApeKI GCWGC 5 cut(s) 171, 279, 426, 442, 457
ApoI RAATTY 1 cut(s) 435
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 4 cut(s) 144, 191, 192, 404
AsuHPI GGTGA 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 195
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 2 cut(s) 152, 161
BanII GRGCYC 1 cut(s) 195
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbvI GCAGC 5 cut(s) 183, 266, 438, 444, 454
BccI CCATC 2 cut(s) 73, 352
BcgI CGANNNNNNTGC 4 cut(s) 101, 135, 278, 312
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 125, 169
BfoI RGCGCY 1 cut(s) 165
BglII AGATCT 1 cut(s) 415
BisI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
BlsI GCNGC 8 cut(s) 46, 161, 173, 281, 301, 428, 444, 459
BmgT120I GGNCC 4 cut(s) 144, 191, 192, 404
BmiI GGNNCC 4 cut(s) 154, 163, 193, 405
BmrI ACTGGG 2 cut(s) 151, 315
BmsI GCATC 1 cut(s) 398
BmuI ACTGGG 2 cut(s) 151, 315
BsaHI GRCGYC 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse118I RCCGGY 1 cut(s) 155
Bse1I ACTGG 4 cut(s) 146, 321, 453, 471
Bse3DI GCAATG 1 cut(s) 483
BseGI GGATG 2 cut(s) 213, 344
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMI GCAATG 1 cut(s) 483
BseNI ACTGG 4 cut(s) 146, 321, 453, 471
BseRI GAGGAG 2 cut(s) 20, 23
BseSI GKGCMC 1 cut(s) 195
BseXI GCAGC 5 cut(s) 183, 266, 438, 444, 454
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 4 cut(s) 98, 146, 193, 406
BshNI GGYRCC 2 cut(s) 152, 161
BsiSI CCGG 1 cut(s) 156
BsiWI CGTACG 1 cut(s) 231
BslI CCNNNNNNNGG 1 cut(s) 326
BsnI GGCC 4 cut(s) 98, 146, 193, 406
Bsp120I GGGCCC 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 7 cut(s) 45, 57, 132, 150, 159, 225, 300
BspANI GGCC 4 cut(s) 98, 146, 193, 406
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 4 cut(s) 154, 163, 193, 405
BspMAI CTGCAG 1 cut(s) 173
BspT107I GGYRCC 2 cut(s) 152, 161
BsrBI CCGCTC 1 cut(s) 134
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 146, 321, 453, 471
BssAI RCCGGY 1 cut(s) 155
BssMI GATC 1 cut(s) 415
BssNI GRCGYC 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 129, 263
BstACI GRCGYC 1 cut(s) 162
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 148, 157
BstF5I GGATG 2 cut(s) 213, 344
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 418
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 6 cut(s) 152, 156, 161, 168, 190, 233
BstSFI CTRYAG 2 cut(s) 125, 169
BstSLI GKGCMC 1 cut(s) 195
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 5 cut(s) 183, 266, 438, 444, 454
BstX2I RGATCY 1 cut(s) 415
BstXI CCANNNNNNTGG 1 cut(s) 188
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 4 cut(s) 98, 146, 193, 406
BtsCI GGATG 2 cut(s) 213, 344
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 2 cut(s) 148, 157
CaiI CAGNNNCTG 1 cut(s) 448
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 4 cut(s) 144, 191, 192, 404
CseI GACGC 1 cut(s) 216
Csp6I GTAC 2 cut(s) 4, 232
CviAII CATG 1 cut(s) 501
CviJI RGCY 9 cut(s) 12, 85, 98, 146, 174, 193, 279, 351, 406
CviKI_1 RGCY 9 cut(s) 12, 85, 98, 146, 174, 193, 279, 351, 406
CviQI GTAC 2 cut(s) 4, 232
DinI GGCGCC 1 cut(s) 163
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 96
Eco24I GRGCYC 1 cut(s) 195
EcoO109I RGGNCCY 2 cut(s) 192, 404
EcoT38I GRGCYC 1 cut(s) 195
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 1 cut(s) 504
FaiI YATR 4 cut(s) 266, 394, 396, 502
FatI CATG 1 cut(s) 500
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
FokI GGATG 2 cut(s) 200, 331
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
FspBI CTAG 1 cut(s) 408
GlaI GCGC 1 cut(s) 163
GluI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 4 cut(s) 98, 146, 193, 406
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 216
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 1 cut(s) 504
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 10
HinfI GANTC 2 cut(s) 116, 316
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 211, 420
Hpy188III TCNNGA 5 cut(s) 76, 137, 217, 413, 519
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 116, 232
HpyAV CCTTC 3 cut(s) 26, 216, 247
HpyCH4III ACNGT 2 cut(s) 129, 263
HpyCH4V TGCA 3 cut(s) 171, 282, 336
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 156, 161, 168, 190, 233
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 1 cut(s) 504
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 2 cut(s) 82, 360
Lsp1109I GCAGC 5 cut(s) 183, 266, 438, 444, 454
LweI GCATC 1 cut(s) 398
MaeI CTAG 1 cut(s) 408
MaeIII GTNAC 2 cut(s) 73, 304
MalI GATC 1 cut(s) 417
MbiI CCGCTC 1 cut(s) 134
MboI GATC 1 cut(s) 415
MboII GAAGA 1 cut(s) 11
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 195
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 2 cut(s) 331, 435
MluNI TGGCCA 1 cut(s) 98
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 5 cut(s) 41, 44, 59, 319, 499
Mox20I TGGCCA 1 cut(s) 98
MroNI GCCGGC 1 cut(s) 155
MscI TGGCCA 1 cut(s) 98
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 1 cut(s) 156
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 6 cut(s) 152, 156, 161, 168, 190, 233
NaeI GCCGGC 1 cut(s) 157
NarI GGCGCC 1 cut(s) 162
NdeI CATATG 1 cut(s) 394
NdeII GATC 1 cut(s) 415
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 1 cut(s) 504
NlaIV GGNNCC 4 cut(s) 154, 163, 193, 405
NmuCI GTSAC 2 cut(s) 73, 304
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 267, 380
PdiI GCCGGC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 316
Pfl23II CGTACG 1 cut(s) 231
PkrI GCNGC 8 cut(s) 46, 161, 173, 281, 301, 428, 444, 459
PleI GAGTC 1 cut(s) 110
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 110
PspLI CGTACG 1 cut(s) 231
PspN4I GGNNCC 4 cut(s) 154, 163, 193, 405
PspOMI GGGCCC 1 cut(s) 191
PspPI GGNCC 4 cut(s) 144, 191, 192, 404
PstI CTGCAG 1 cut(s) 173
PstNI CAGNNNCTG 1 cut(s) 448
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 279
RsaI GTAC 2 cut(s) 5, 233
RsaNI GTAC 2 cut(s) 4, 232
SatI GCNGC 8 cut(s) 45, 160, 172, 280, 300, 427, 443, 458
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 4 cut(s) 144, 191, 192, 404
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 195
SetI ASST 6 cut(s) 14, 87, 190, 281, 311, 353
SfaNI GCATC 1 cut(s) 398
SfcI CTRYAG 2 cut(s) 125, 169
SfoI GGCGCC 1 cut(s) 163
Sse9I AATT 2 cut(s) 331, 435
SsiI CCGC 7 cut(s) 45, 57, 132, 150, 159, 225, 300
SspDI GGCGCC 1 cut(s) 161
SspMI CTAG 1 cut(s) 408
TaaI ACNGT 2 cut(s) 129, 263
TaqI TCGA 4 cut(s) 138, 288, 374, 383
TaqII GACCGA 1 cut(s) 284
TasI AATT 2 cut(s) 331, 435
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 47, 162, 302
TfiI GAWTC 1 cut(s) 316
TscAI CASTG 1 cut(s) 301
TseFI GTSAC 2 cut(s) 73, 304
TseI GCWGC 5 cut(s) 171, 279, 426, 442, 457
Tsp45I GTSAC 2 cut(s) 73, 304
TspDTI ATGAA 1 cut(s) 369
TspRI CASTG 1 cut(s) 301
XapI RAATTY 1 cut(s) 435
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.