pycom09g15960

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0011944
Physical Location & Seq
Reverse (-)
16049014 .. 16050118
1105 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g15960.1

Sequence Viewer

Length: 531 bp
ATGCACAAAAGCATCGGTTTATCTTTGATCCTTTCACTGACCCTCTTCTGCCACTTGATCCCGCCGTCCAAGGCCAAGGACGGCGTCGTCACGAGGGAGCTCTGGTGCGTGGCCAAGAACAACGCGGAGGACGCCCCCCTGGTGACGGCGCTGAACTGGGCCTGCGGGCCTGGTGGAGCCGACTGCCGCCCGATCCAGCAAGGTGGACCCTGCTACGACCCCGTCGACATCCAGAACACTGCCTCCTACGCTTTCAACGACTACTTTCTGAAACACGGCATGACCGCCGATAGTTGCAGCTTTGATAACACTGCCGCGCTTACTTCGCTGAATCCCAGTCATGATAAGTGCAAATTCCCATCCAGCTTATCAGCTAGCAATGCAAGCATCTCCAGTGCATCAACAGCAATTGGAAATGGAATGGGACGTCGTGAAGATTTGAACGGCAGCAATATGATTTCTGAGCACTGGATTTGGCCCGTCCTTACCAGTTTTCTGGTCATTGCATATAATACATGGGCAATGGGTTAG

Protein Analysis

177

Amino Acids

18.87

Weight (kDa)

5.88

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 34 - 105 3.3e-19 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58100 AT3G58100
fragaria_vesca FvH4_6g27020 FvH4_6g27020 FvH4_6g27020
malus_domestica MD09G1240800.v1.1 MD17G1233700.v1.1
prunus_persica Prupe.3G125100_v2.0.a1
pyrus_communis pycom09g15960 pycom17g23680
rosa_chinensis RchiOBHm_Chr2g0128881
rosa_laevigata RLG00000019037
rosa_multiflora Rmu_sc0001643.1_g000031
rosa_roxburghii Rroxscaffold_2G00115140
rosa_rugosa Rorug02G0282100
rosa_samantha Rh2AG334500 Rh2CG321500 Rh2DG359200
rosa_wichuraiana Rw2G027070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 1 cut(s) 430
AccI GTMKAC 1 cut(s) 225
AccII CGCG 2 cut(s) 125, 317
AciI CCGC 6 cut(s) 62, 125, 165, 187, 285, 315
AclWI GGATC 3 cut(s) 22, 52, 187
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 353
AcyI GRCGYC 3 cut(s) 84, 132, 427
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 256, 442
AjnI CCWGG 2 cut(s) 138, 169
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 100, 300, 366, 374
AluI AGCT 4 cut(s) 100, 300, 366, 374
Alw21I GWGCWC 2 cut(s) 102, 468
AlwI GGATC 3 cut(s) 22, 52, 187
AoxI GGCC 5 cut(s) 72, 111, 159, 167, 476
ApeKI GCWGC 2 cut(s) 297, 447
ApoI RAATTY 1 cut(s) 353
AspLEI GCGC 2 cut(s) 151, 319
AspS9I GGNCC 4 cut(s) 159, 167, 206, 477
AsuHPI GGTGA 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 374
AvaII GGWCC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 113
BanII GRGCYC 1 cut(s) 102
BauI CACGAG 1 cut(s) 91
Bbv12I GWGCWC 2 cut(s) 102, 468
BbvI GCAGC 2 cut(s) 309, 459
BccI CCATC 1 cut(s) 367
BceAI ACGGC 5 cut(s) 49, 97, 162, 292, 460
BciT130I CCWGG 2 cut(s) 140, 171
BfaI CTAG 1 cut(s) 375
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 4 cut(s) 187, 298, 315, 448
BlsI GCNGC 4 cut(s) 188, 299, 316, 449
Bme1390I CCNGG 2 cut(s) 140, 171
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 4 cut(s) 159, 167, 206, 477
BmiI GGNNCC 2 cut(s) 178, 208
BmrFI CCNGG 2 cut(s) 140, 171
BmrI ACTGGG 2 cut(s) 166, 330
BmsI GCATC 3 cut(s) 21, 396, 407
BmtI GCTAGC 1 cut(s) 378
BmuI ACTGGG 2 cut(s) 166, 330
BpmI CTGGAG 1 cut(s) 376
BsaHI GRCGYC 3 cut(s) 84, 132, 427
BsaJI CCNNGG 3 cut(s) 69, 75, 138
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 5 cut(s) 161, 336, 393, 473, 489
Bse3DI GCAATG 3 cut(s) 385, 501, 528
BseBI CCWGG 2 cut(s) 140, 171
BseDI CCNNGG 3 cut(s) 69, 75, 138
BseGI GGATG 2 cut(s) 228, 359
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 3 cut(s) 385, 501, 528
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 5 cut(s) 161, 336, 393, 473, 489
BseXI GCAGC 2 cut(s) 309, 459
Bsh1236I CGCG 2 cut(s) 125, 317
BshFI GGCC 5 cut(s) 74, 113, 161, 169, 478
BsiHKAI GWGCWC 2 cut(s) 102, 468
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 5 cut(s) 74, 113, 161, 169, 478
Bsp1286I GDGCHC 2 cut(s) 102, 468
Bsp143I GATC 3 cut(s) 27, 57, 192
BspACI CCGC 6 cut(s) 62, 125, 165, 187, 285, 315
BspANI GGCC 5 cut(s) 74, 113, 161, 169, 478
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 2 cut(s) 125, 317
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 178, 208
BspOI GCTAGC 1 cut(s) 378
BspPI GGATC 3 cut(s) 22, 52, 187
BsrDI GCAATG 3 cut(s) 385, 501, 528
BsrI ACTGG 5 cut(s) 161, 336, 393, 473, 489
BssECI CCNNGG 3 cut(s) 69, 75, 138
BssMI GATC 3 cut(s) 27, 57, 192
BssNI GRCGYC 3 cut(s) 84, 132, 427
BssSI CACGAG 1 cut(s) 91
BssT1I CCWWGG 2 cut(s) 69, 75
Bst2BI CACGAG 1 cut(s) 91
Bst2UI CCWGG 2 cut(s) 140, 171
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 3 cut(s) 84, 132, 427
BstC8I GCNNGC 4 cut(s) 163, 167, 376, 385
BstDEI CTNAG 1 cut(s) 462
BstF5I GGATG 2 cut(s) 228, 359
BstFNI CGCG 2 cut(s) 125, 317
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 151, 319
BstKTI GATC 3 cut(s) 30, 60, 195
BstMBI GATC 3 cut(s) 27, 57, 192
BstMWI GCNNNNNNNGC 6 cut(s) 131, 248, 325, 380, 384, 404
BstNI CCWGG 2 cut(s) 140, 171
BstSCI CCNGG 2 cut(s) 138, 169
BstUI CGCG 2 cut(s) 125, 317
BstV1I GCAGC 2 cut(s) 309, 459
BstXI CCANNNNNNTGG 2 cut(s) 203, 496
BsuRI GGCC 5 cut(s) 74, 113, 161, 169, 478
BtsCI GGATG 2 cut(s) 228, 359
BtsI GCAGTG 2 cut(s) 237, 309
BtsIMutI CAGTG 5 cut(s) 35, 237, 309, 400, 466
Cac8I GCNNGC 4 cut(s) 163, 167, 376, 385
CciI TCATGA 1 cut(s) 340
CfoI GCGC 2 cut(s) 151, 319
Cfr13I GGNCC 4 cut(s) 159, 167, 206, 477
CseI GACGC 2 cut(s) 73, 140
CviAII CATG 3 cut(s) 280, 341, 516
DdeI CTNAG 1 cut(s) 462
DpnI GATC 3 cut(s) 29, 59, 194
DpnII GATC 3 cut(s) 27, 57, 192
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 69, 75
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 138, 169
EcoT14I CCWWGG 2 cut(s) 69, 75
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 69, 75
FaeI CATG 3 cut(s) 283, 344, 519
FaiI YATR 6 cut(s) 281, 342, 455, 508, 510, 517
FaqI GGGAC 1 cut(s) 438
FatI CATG 3 cut(s) 279, 340, 515
FauI CCCGC 2 cut(s) 69, 158
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 187, 298, 315, 448
FokI GGATG 2 cut(s) 215, 346
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 187, 298, 315, 448
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 150, 318
GluI GCNGC 4 cut(s) 187, 298, 315, 448
GsuI CTGGAG 1 cut(s) 376
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 5 cut(s) 74, 113, 161, 169, 478
HgaI GACGC 2 cut(s) 73, 140
HhaI GCGC 2 cut(s) 151, 319
Hin1I GRCGYC 3 cut(s) 84, 132, 427
Hin1II CATG 3 cut(s) 283, 344, 519
Hin6I GCGC 2 cut(s) 149, 317
HinP1I GCGC 2 cut(s) 149, 317
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 1 cut(s) 331
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 206, 226
Hpy188I TCNGA 2 cut(s) 270, 463
Hpy188III TCNNGA 4 cut(s) 91, 232, 341, 431
Hpy8I GTNNAC 2 cut(s) 206, 226
Hpy99I CGWCG 3 cut(s) 89, 227, 432
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 6 cut(s) 4, 297, 351, 383, 398, 506
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 248, 325, 380, 384, 404
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 3 cut(s) 84, 132, 427
Hsp92II CATG 3 cut(s) 283, 344, 519
HspAI GCGC 2 cut(s) 149, 317
Kzo9I GATC 3 cut(s) 27, 57, 192
LmnI GCTCC 2 cut(s) 97, 176
Lsp1109I GCAGC 2 cut(s) 309, 459
LweI GCATC 3 cut(s) 21, 396, 407
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 2 cut(s) 88, 142
MalI GATC 3 cut(s) 29, 59, 194
MboI GATC 3 cut(s) 27, 57, 192
MboII GAAGA 2 cut(s) 37, 446
MfeI CAATTG 1 cut(s) 408
MhlI GDGCHC 2 cut(s) 102, 468
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 2 cut(s) 353, 408
MluNI TGGCCA 1 cut(s) 113
MnlI CCTC 4 cut(s) 53, 87, 121, 253
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 113
MspR9I CCNGG 2 cut(s) 140, 171
MunI CAATTG 1 cut(s) 408
MvaI CCWGG 2 cut(s) 140, 171
MvnI CGCG 2 cut(s) 125, 317
MwoI GCNNNNNNNGC 6 cut(s) 131, 248, 325, 380, 384, 404
NdeII GATC 3 cut(s) 27, 57, 192
NheI GCTAGC 1 cut(s) 374
NlaIII CATG 3 cut(s) 283, 344, 519
NlaIV GGNNCC 2 cut(s) 178, 208
NmuCI GTSAC 2 cut(s) 88, 142
PagI TCATGA 1 cut(s) 340
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 255
PfeI GAWTC 1 cut(s) 331
PflFI GACNNNGTC 2 cut(s) 83, 221
PkrI GCNGC 4 cut(s) 188, 299, 316, 449
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 138, 169
PspGI CCWGG 2 cut(s) 138, 169
PspN4I GGNNCC 2 cut(s) 178, 208
PspPI GGNCC 4 cut(s) 159, 167, 206, 477
PsyI GACNNNGTC 2 cut(s) 83, 221
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 224
SatI GCNGC 4 cut(s) 187, 298, 315, 448
Sau3AI GATC 3 cut(s) 27, 57, 192
Sau96I GGNCC 4 cut(s) 159, 167, 206, 477
ScrFI CCNGG 2 cut(s) 140, 171
SduI GDGCHC 2 cut(s) 102, 468
SetI ASST 6 cut(s) 102, 205, 302, 368, 376, 430
SfaNI GCATC 3 cut(s) 21, 396, 407
SinI GGWCC 1 cut(s) 206
Sse9I AATT 2 cut(s) 353, 408
SsiI CCGC 6 cut(s) 62, 125, 165, 187, 285, 315
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 2 cut(s) 138, 169
StyI CCWWGG 2 cut(s) 69, 75
TaiI ACGT 1 cut(s) 430
TaqI TCGA 1 cut(s) 225
TasI AATT 2 cut(s) 353, 408
TauI GCSGC 2 cut(s) 189, 317
TfiI GAWTC 1 cut(s) 331
TscAI CASTG 5 cut(s) 42, 244, 316, 400, 473
TseFI GTSAC 2 cut(s) 88, 142
TseI GCWGC 2 cut(s) 297, 447
Tsp45I GTSAC 2 cut(s) 88, 142
TspRI CASTG 5 cut(s) 42, 244, 316, 400, 473
Tth111I GACNNNGTC 2 cut(s) 83, 221
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 353
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 375
ZraI GACGTC 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.