MD09G1240800.v1.1

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011944
Physical Location & Seq
Reverse (-)
30775915 .. 30777737
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1240800.v1.1.491

Sequence Viewer

Length: 531 bp
ATGCACAAAAGCATCGGTTTATCTTTGATCCTTTCACTGACCCTCTTCTGCCACTTGATCCCGCCGTCCAAGGCCAAGGAAGGCGTCGTCACGATGGAGCTCTGGTGCGTGGCCAAGAACAACGCGGAGGACGCCCCCCTGCTGACGGCGCTGAACTGGGCCTGCGGGCCTGGTGGAGTCGACTGCCGCCCAATCCAGCAAGGTGGACCCTGCTACGACCCCGCCGACATCCAGAACACTGCCTCCTACGCTTTCAACGACTACTTTCTGAAACACGGCATGACCGCCGATAGTTGCAGCTTTGATAACACTGCGGCGCTTACTTCTCTGAATCCCAGTCATGATAAGTGCAAATTGCCATCCAGCTTATCAGCTAGCAATGCAAGCATCTCCAGTGCATCAACAGCAATTGGAAATGGAATGGGACGTAGTGAAGATTTGAACGGCAGCAATAAGATTTCTGAGCACTGGATTTGGCCCGTCCTTACCAGTTTTCTGGTCATTGCATATAATACATGGGCAATGGGTTAG

Protein Analysis

177

Amino Acids

18.77

Weight (kDa)

5.62

Isoelectric Point (pI)

36.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 34 - 105 4.3e-19 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58100 AT3G58100
fragaria_vesca FvH4_6g27020 FvH4_6g27020 FvH4_6g27020
malus_domestica MD09G1240800.v1.1 MD17G1233700.v1.1
prunus_persica Prupe.3G125100_v2.0.a1
pyrus_communis pycom09g15960 pycom17g23680
rosa_chinensis RchiOBHm_Chr2g0128881
rosa_laevigata RLG00000019037
rosa_multiflora Rmu_sc0001643.1_g000031
rosa_roxburghii Rroxscaffold_2G00115140
rosa_rugosa Rorug02G0282100
rosa_samantha Rh2AG334500 Rh2CG321500 Rh2DG359200
rosa_wichuraiana Rw2G027070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 125
AciI CCGC 7 cut(s) 62, 125, 165, 187, 222, 285, 314
AclWI GGATC 2 cut(s) 22, 52
AcoI YGGCCR 1 cut(s) 111
AcyI GRCGYC 2 cut(s) 84, 132
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 256, 442
AjnI CCWGG 1 cut(s) 169
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 4 cut(s) 100, 300, 366, 374
AluI AGCT 4 cut(s) 100, 300, 366, 374
Alw21I GWGCWC 2 cut(s) 102, 468
AlwI GGATC 2 cut(s) 22, 52
AoxI GGCC 5 cut(s) 72, 111, 159, 167, 476
ApeKI GCWGC 2 cut(s) 297, 447
AspLEI GCGC 2 cut(s) 151, 319
AspS9I GGNCC 4 cut(s) 159, 167, 206, 477
AsuNHI GCTAGC 1 cut(s) 374
AvaII GGWCC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 113
BanII GRGCYC 1 cut(s) 102
Bbv12I GWGCWC 2 cut(s) 102, 468
BbvI GCAGC 2 cut(s) 309, 459
BccI CCATC 2 cut(s) 88, 367
BceAI ACGGC 4 cut(s) 49, 162, 292, 460
BciT130I CCWGG 1 cut(s) 171
BfaI CTAG 1 cut(s) 375
BfoI RGCGCY 2 cut(s) 152, 320
BisI GCNGC 4 cut(s) 187, 298, 315, 448
BlsI GCNGC 4 cut(s) 188, 299, 316, 449
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 4 cut(s) 159, 167, 206, 477
BmiI GGNNCC 1 cut(s) 208
BmrFI CCNGG 1 cut(s) 171
BmrI ACTGGG 2 cut(s) 166, 330
BmsI GCATC 3 cut(s) 21, 396, 407
BmtI GCTAGC 1 cut(s) 378
BmuI ACTGGG 2 cut(s) 166, 330
BpmI CTGGAG 1 cut(s) 376
BsaHI GRCGYC 2 cut(s) 84, 132
BsaJI CCNNGG 2 cut(s) 69, 75
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 5 cut(s) 161, 336, 393, 473, 489
Bse3DI GCAATG 3 cut(s) 385, 501, 528
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 69, 75
BseGI GGATG 2 cut(s) 228, 359
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 3 cut(s) 385, 501, 528
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 5 cut(s) 161, 336, 393, 473, 489
BseXI GCAGC 2 cut(s) 309, 459
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 5 cut(s) 74, 113, 161, 169, 478
BsiHKAI GWGCWC 2 cut(s) 102, 468
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 5 cut(s) 74, 113, 161, 169, 478
Bsp1286I GDGCHC 2 cut(s) 102, 468
Bsp143I GATC 2 cut(s) 27, 57
BspACI CCGC 7 cut(s) 62, 125, 165, 187, 222, 285, 314
BspANI GGCC 5 cut(s) 74, 113, 161, 169, 478
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 208
BspOI GCTAGC 1 cut(s) 378
BspPI GGATC 2 cut(s) 22, 52
BsrDI GCAATG 3 cut(s) 385, 501, 528
BsrI ACTGG 5 cut(s) 161, 336, 393, 473, 489
BssECI CCNNGG 2 cut(s) 69, 75
BssMI GATC 2 cut(s) 27, 57
BssNI GRCGYC 2 cut(s) 84, 132
BssT1I CCWWGG 2 cut(s) 69, 75
Bst2UI CCWGG 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 2 cut(s) 84, 132
BstC8I GCNNGC 4 cut(s) 163, 167, 376, 385
BstDEI CTNAG 1 cut(s) 462
BstF5I GGATG 2 cut(s) 228, 359
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 2 cut(s) 152, 320
BstHHI GCGC 2 cut(s) 151, 319
BstKTI GATC 2 cut(s) 30, 60
BstMBI GATC 2 cut(s) 27, 57
BstMWI GCNNNNNNNGC 6 cut(s) 131, 148, 248, 380, 384, 404
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 2 cut(s) 309, 459
BstXI CCANNNNNNTGG 2 cut(s) 203, 496
BsuRI GGCC 5 cut(s) 74, 113, 161, 169, 478
BtsCI GGATG 2 cut(s) 228, 359
BtsI GCAGTG 2 cut(s) 237, 309
BtsIMutI CAGTG 5 cut(s) 35, 237, 309, 400, 466
Cac8I GCNNGC 4 cut(s) 163, 167, 376, 385
CciI TCATGA 1 cut(s) 340
CfoI GCGC 2 cut(s) 151, 319
Cfr13I GGNCC 4 cut(s) 159, 167, 206, 477
CseI GACGC 2 cut(s) 73, 140
CviAII CATG 3 cut(s) 280, 341, 516
CviJI RGCY 9 cut(s) 74, 100, 113, 161, 169, 300, 366, 374, 478
CviKI_1 RGCY 9 cut(s) 74, 100, 113, 161, 169, 300, 366, 374, 478
DdeI CTNAG 1 cut(s) 462
DpnI GATC 2 cut(s) 29, 59
DpnII GATC 2 cut(s) 27, 57
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 69, 75
Eco24I GRGCYC 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 2 cut(s) 69, 75
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 2 cut(s) 69, 75
FaeI CATG 3 cut(s) 283, 344, 519
FaiI YATR 5 cut(s) 281, 342, 508, 510, 517
FaqI GGGAC 1 cut(s) 438
FatI CATG 3 cut(s) 279, 340, 515
FauI CCCGC 3 cut(s) 69, 158, 229
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 187, 298, 315, 448
FokI GGATG 2 cut(s) 215, 346
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 187, 298, 315, 448
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 150, 318
GluI GCNGC 4 cut(s) 187, 298, 315, 448
GsuI CTGGAG 1 cut(s) 376
HaeII RGCGCY 2 cut(s) 152, 320
HaeIII GGCC 5 cut(s) 74, 113, 161, 169, 478
HgaI GACGC 2 cut(s) 73, 140
HhaI GCGC 2 cut(s) 151, 319
Hin1I GRCGYC 2 cut(s) 84, 132
Hin1II CATG 3 cut(s) 283, 344, 519
Hin6I GCGC 2 cut(s) 149, 317
HinP1I GCGC 2 cut(s) 149, 317
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 2 cut(s) 177, 331
Hpy166II GTNNAC 2 cut(s) 181, 206
Hpy188I TCNGA 3 cut(s) 270, 330, 463
Hpy188III TCNNGA 3 cut(s) 91, 232, 341
Hpy8I GTNNAC 2 cut(s) 181, 206
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 74
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 6 cut(s) 4, 297, 351, 383, 398, 506
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 148, 248, 380, 384, 404
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 2 cut(s) 84, 132
Hsp92II CATG 3 cut(s) 283, 344, 519
HspAI GCGC 2 cut(s) 149, 317
Kzo9I GATC 2 cut(s) 27, 57
LmnI GCTCC 1 cut(s) 97
Lsp1109I GCAGC 2 cut(s) 309, 459
LweI GCATC 3 cut(s) 21, 396, 407
MaeI CTAG 1 cut(s) 375
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 88
MalI GATC 2 cut(s) 29, 59
MboI GATC 2 cut(s) 27, 57
MboII GAAGA 2 cut(s) 37, 446
MfeI CAATTG 1 cut(s) 408
MhlI GDGCHC 2 cut(s) 102, 468
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 2 cut(s) 353, 408
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 1 cut(s) 186
MnlI CCTC 3 cut(s) 53, 121, 253
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 171
MunI CAATTG 1 cut(s) 408
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 6 cut(s) 131, 148, 248, 380, 384, 404
NdeII GATC 2 cut(s) 27, 57
NheI GCTAGC 1 cut(s) 374
NlaIII CATG 3 cut(s) 283, 344, 519
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 88
PagI TCATGA 1 cut(s) 340
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 255
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 4 cut(s) 188, 299, 316, 449
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 4 cut(s) 159, 167, 206, 477
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 179
SatI GCNGC 4 cut(s) 187, 298, 315, 448
Sau3AI GATC 2 cut(s) 27, 57
Sau96I GGNCC 4 cut(s) 159, 167, 206, 477
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 2 cut(s) 102, 468
SetI ASST 6 cut(s) 102, 205, 302, 368, 376, 430
SfaNI GCATC 3 cut(s) 21, 396, 407
SinI GGWCC 1 cut(s) 206
Sse9I AATT 2 cut(s) 353, 408
SsiI CCGC 7 cut(s) 62, 125, 165, 187, 222, 285, 314
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 2 cut(s) 69, 75
TaiI ACGT 1 cut(s) 430
TaqI TCGA 1 cut(s) 180
TasI AATT 2 cut(s) 353, 408
TauI GCSGC 2 cut(s) 189, 317
TfiI GAWTC 1 cut(s) 331
TscAI CASTG 5 cut(s) 42, 244, 316, 400, 473
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 297, 447
Tsp45I GTSAC 1 cut(s) 88
TspRI CASTG 5 cut(s) 42, 244, 316, 400, 473
VpaK11BI GGWCC 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.