pycom17g23680

Glucan endo-1,3-beta-glucosidase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0011944
Physical Location & Seq
Reverse (-)
21974239 .. 21975759
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g23680.1

Sequence Viewer

Length: 531 bp
ATGCACAAAAACATCGCTTTCTCTTTCATCCTCTCACTCGCCCTCTTCTGCCTTTTGATCCTGCCTTCCACGGCCAAGGACGGCTTGGTTACGACGGAGCTGTGGTGCGTGGCCAAGAACAACGCGGAGGACGCCCCCCTGCTGACGGCGCTGAACTGGGCATGCGGGCCTGGTGGGGCGGACTGCAGCCCAATCCAGCAAGGTGGGCGCTGCTACGACCCAACCGACATCCAGAACACTGCCTCCTACGCTTTCAACGACTACTTTCTGAAACACGGCATGACCGACGATAGTTGCAACTTTGATAACACTGCCGCACTCACTTCTCTGAATCCCAGTCATGATAAGTGCAAACTCCCATCCAGCTTATCAGCGAGCAATGCAAGCATCTCCAGTCCATCAACAGCATATGAAAATGGAATGGGACCTAGTGAAAATTTGAACGGCAGCAATAAGATTTCTGAGCAATGGATTTGGCCCCTCGTTACCAGTTTTCTGGTCATTGCATATAATACATGGGCAATGGGTTAG

Protein Analysis

177

Amino Acids

18.93

Weight (kDa)

4.63

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
X8 PF07983 34 - 105 1.2e-19 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G58100 AT3G58100
fragaria_vesca FvH4_6g27020 FvH4_6g27020 FvH4_6g27020
malus_domestica MD09G1240800.v1.1 MD17G1233700.v1.1
prunus_persica Prupe.3G125100_v2.0.a1
pyrus_communis pycom09g15960 pycom17g23680
rosa_chinensis RchiOBHm_Chr2g0128881
rosa_laevigata RLG00000019037
rosa_multiflora Rmu_sc0001643.1_g000031
rosa_roxburghii Rroxscaffold_2G00115140
rosa_rugosa Rorug02G0282100
rosa_samantha Rh2AG334500 Rh2CG321500 Rh2DG359200
rosa_wichuraiana Rw2G027070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 125
AciI CCGC 4 cut(s) 125, 165, 179, 315
AclWI GGATC 1 cut(s) 52
AcoI YGGCCR 2 cut(s) 72, 111
AcsI RAATTY 1 cut(s) 436
AcyI GRCGYC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 145
AgsI TTSAA 2 cut(s) 256, 442
AjnI CCWGG 1 cut(s) 169
AloI GAACNNNNNNTCC 2 cut(s) 227, 259
AluBI AGCT 2 cut(s) 100, 366
AluI AGCT 2 cut(s) 100, 366
AlwI GGATC 1 cut(s) 52
AoxI GGCC 4 cut(s) 72, 111, 167, 476
ApeKI GCWGC 3 cut(s) 186, 210, 447
ApoI RAATTY 1 cut(s) 436
AspLEI GCGC 2 cut(s) 151, 210
AspS9I GGNCC 3 cut(s) 167, 425, 477
AvaII GGWCC 1 cut(s) 425
BalI TGGCCA 1 cut(s) 113
BbvI GCAGC 3 cut(s) 197, 198, 459
BccI CCATC 2 cut(s) 367, 406
BceAI ACGGC 5 cut(s) 87, 97, 162, 292, 460
BciT130I CCWGG 1 cut(s) 171
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 184
BfoI RGCGCY 2 cut(s) 152, 211
BisI GCNGC 4 cut(s) 187, 211, 315, 448
BlsI GCNGC 4 cut(s) 188, 212, 316, 449
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 3 cut(s) 167, 425, 477
BmiI GGNNCC 2 cut(s) 426, 479
BmrFI CCNGG 1 cut(s) 171
BmrI ACTGGG 2 cut(s) 166, 330
BmsI GCATC 1 cut(s) 396
BmuI ACTGGG 2 cut(s) 166, 330
BpmI CTGGAG 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 69, 75
BsaXI ACNNNNNCTCC 4 cut(s) 89, 119, 227, 257
Bsc4I CCNNNNNNNGG 1 cut(s) 145
Bse1I ACTGG 4 cut(s) 161, 336, 393, 489
Bse3DI GCAATG 4 cut(s) 385, 473, 501, 528
BseBI CCWGG 1 cut(s) 171
BseDI CCNNGG 2 cut(s) 69, 75
BseGI GGATG 3 cut(s) 27, 228, 359
BseLI CCNNNNNNNGG 1 cut(s) 145
BseMI GCAATG 4 cut(s) 385, 473, 501, 528
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 4 cut(s) 161, 336, 393, 489
BseXI GCAGC 3 cut(s) 197, 198, 459
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 4 cut(s) 74, 113, 169, 478
BslFI GGGAC 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 438
BsnI GGCC 4 cut(s) 74, 113, 169, 478
Bsp143I GATC 1 cut(s) 57
BspACI CCGC 4 cut(s) 125, 165, 179, 315
BspANI GGCC 4 cut(s) 74, 113, 169, 478
BspCNI CTCAG 1 cut(s) 454
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 1 cut(s) 340
BspLI GGNNCC 2 cut(s) 426, 479
BspMAI CTGCAG 1 cut(s) 188
BspPI GGATC 1 cut(s) 52
BsrDI GCAATG 4 cut(s) 385, 473, 501, 528
BsrI ACTGG 4 cut(s) 161, 336, 393, 489
BssECI CCNNGG 2 cut(s) 69, 75
BssMI GATC 1 cut(s) 57
BssNI GRCGYC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 50
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 4 cut(s) 163, 167, 376, 385
BstDEI CTNAG 1 cut(s) 462
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 3 cut(s) 27, 228, 359
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 2 cut(s) 152, 211
BstHHI GCGC 2 cut(s) 151, 210
BstKTI GATC 1 cut(s) 60
BstMBI GATC 1 cut(s) 57
BstMWI GCNNNNNNNGC 6 cut(s) 131, 148, 205, 248, 380, 384
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 184
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 3 cut(s) 197, 198, 459
BstXI CCANNNNNNTGG 2 cut(s) 203, 496
BsuRI GGCC 4 cut(s) 74, 113, 169, 478
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 3 cut(s) 27, 228, 359
BtsI GCAGTG 2 cut(s) 237, 309
BtsIMutI CAGTG 2 cut(s) 237, 309
Cac8I GCNNGC 4 cut(s) 163, 167, 376, 385
CciI TCATGA 1 cut(s) 340
CfoI GCGC 2 cut(s) 151, 210
Cfr13I GGNCC 3 cut(s) 167, 425, 477
CseI GACGC 1 cut(s) 140
CviAII CATG 4 cut(s) 162, 280, 341, 516
CviJI RGCY 8 cut(s) 74, 84, 100, 113, 169, 189, 366, 478
CviKI_1 RGCY 8 cut(s) 74, 84, 100, 113, 169, 189, 366, 478
DdeI CTNAG 1 cut(s) 462
DpnI GATC 1 cut(s) 59
DpnII GATC 1 cut(s) 57
EaeI YGGCCR 2 cut(s) 72, 111
Eam1104I CTCTTC 1 cut(s) 50
EarI CTCTTC 1 cut(s) 50
EciI GGCGGA 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 75
Eco47I GGWCC 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 75
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 165, 283, 344, 519
FaiI YATR 8 cut(s) 163, 281, 342, 409, 411, 508, 510, 517
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 438
FatI CATG 4 cut(s) 161, 279, 340, 515
FauI CCCGC 1 cut(s) 158
FauNDI CATATG 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 187, 211, 315, 448
FokI GGATG 3 cut(s) 14, 215, 346
Fsp4HI GCNGC 4 cut(s) 187, 211, 315, 448
FspBI CTAG 1 cut(s) 429
GlaI GCGC 2 cut(s) 150, 209
GluI GCNGC 4 cut(s) 187, 211, 315, 448
GsuI CTGGAG 1 cut(s) 376
HaeII RGCGCY 2 cut(s) 152, 211
HaeIII GGCC 4 cut(s) 74, 113, 169, 478
HgaI GACGC 1 cut(s) 140
HhaI GCGC 2 cut(s) 151, 210
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 4 cut(s) 165, 283, 344, 519
Hin6I GCGC 2 cut(s) 149, 208
HinP1I GCGC 2 cut(s) 149, 208
HinfI GANTC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 270, 330, 463
Hpy188III TCNNGA 2 cut(s) 232, 341
Hpy99I CGWCG 2 cut(s) 97, 290
HpyAV CCTTC 1 cut(s) 75
HpyCH4V TGCA 6 cut(s) 4, 186, 297, 351, 383, 506
HpyF10VI GCNNNNNNNGC 6 cut(s) 131, 148, 205, 248, 380, 384
HpyF3I CTNAG 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 4 cut(s) 165, 283, 344, 519
HspAI GCGC 2 cut(s) 149, 208
Kzo9I GATC 1 cut(s) 57
LmnI GCTCC 1 cut(s) 97
Lsp1109I GCAGC 3 cut(s) 197, 198, 459
LweI GCATC 1 cut(s) 396
MaeI CTAG 1 cut(s) 429
MaeIII GTNAC 2 cut(s) 88, 484
MalI GATC 1 cut(s) 59
MboI GATC 1 cut(s) 57
MboII GAAGA 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 1 cut(s) 436
MluNI TGGCCA 1 cut(s) 113
MnlI CCTC 5 cut(s) 41, 53, 121, 253, 491
Mox20I TGGCCA 1 cut(s) 113
MscI TGGCCA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 6 cut(s) 131, 148, 205, 248, 380, 384
NdeI CATATG 1 cut(s) 409
NdeII GATC 1 cut(s) 57
NlaIII CATG 4 cut(s) 165, 283, 344, 519
NlaIV GGNNCC 2 cut(s) 426, 479
NspI RCATGY 1 cut(s) 165
PaeI GCATGC 1 cut(s) 165
PagI TCATGA 1 cut(s) 340
PcsI WCGNNNNNNNCGW 3 cut(s) 222, 255, 282
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 4 cut(s) 188, 212, 316, 449
PpuMI RGGWCCY 1 cut(s) 425
Psp5II RGGWCCY 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 426, 479
PspPI GGNCC 3 cut(s) 167, 425, 477
PspPPI RGGWCCY 1 cut(s) 425
PstI CTGCAG 1 cut(s) 188
SatI GCNGC 4 cut(s) 187, 211, 315, 448
Sau3AI GATC 1 cut(s) 57
Sau96I GGNCC 3 cut(s) 167, 425, 477
ScrFI CCNGG 1 cut(s) 171
SetI ASST 4 cut(s) 102, 205, 368, 430
SfaNI GCATC 1 cut(s) 396
SfcI CTRYAG 1 cut(s) 184
SinI GGWCC 1 cut(s) 425
SphI GCATGC 1 cut(s) 165
Sse9I AATT 1 cut(s) 436
SsiI CCGC 4 cut(s) 125, 165, 179, 315
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 75
TaqII GACCGA 1 cut(s) 299
TasI AATT 1 cut(s) 436
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 1 cut(s) 331
TscAI CASTG 2 cut(s) 244, 316
TseI GCWGC 3 cut(s) 186, 210, 447
TspDTI ATGAA 2 cut(s) 16, 426
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 2 cut(s) 244, 316
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 1 cut(s) 436
XceI RCATGY 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 82
XspI CTAG 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.