FvH4_6g27080

Protein of unknown function (DUF455)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0014019
Physical Location & Seq
Forward (+)
20784377 .. 20791370
6994 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27080.t1

Sequence Viewer

Length: 1107 bp
ATGCAGCGTATGAGACTCCACCACATGCTCTCTCCAGCACCGCACTGCCTCACAATCAAGTCCTTTTCTTCTTCGCAACATCACTCACCATGGTCTGGTCTCGAAACTTGGAGGAATAGCCCCCTCAATGACAACCGTTGCTGGGGACCCAAGGGCCCCCAAACTCACCCCTCTTCCCTTCACCCTGACGCTCCAATGCTGCCGTCATCCGCTTCCTCTCTCGCCGAGCTTGGTGCTCTCGTTCTCTCCACCCCTGACCCCCTCGCCAAGTCCCACCTCTCCCACCTCGCTTTCTCCACGTGGCGCCAACGCAACCTCCCACTTGGCTCATGCCCACCCCCTCCTCGTCCCGCCCGCCCTCTTAAACCCCAACTGGTGCATCCGAAAGAAATTCCGGCTCCCAAGAACTCGGGATTGCCGCTGAATGCGTATATGCTACATAATCTGGCCCATGTGGAGCTCAATGCTATTGATTTAGCGTGGGACACTGTTGTCCGTTTTTCTCCATTTAGTGACATTCTAGGAGAAGGGTTTTTCGCTGACTTTGCTCATGTTGCCGACGATGAGAGCCGCCATTTTGCTTGGTGTTCTCAGAGGCTTGCCGAACTTGGTTTCAAGTATGGAGATATGCCTGCTCATAATCTGCTCTGGAGGGAGTGTGAGAAGTCATCTGACAATGTTGCTGCACGTTTGGCTGTCATTCCTCTGGTCCAGGAGGCCAGAGGACTGGATGCCGGACCACGGCTTGTGCAAAAATTGGTTGGGTTTGGAGATCTCAGGACATCTGAAATCGTTGCTAGGATTGCTGATGAAGAAGTGACACATGTAGCTGTTGGTGTCCACTGGTTTGTCAGTGTCTGTCAGAAATTAGGTTGCGCCCCATCTTCTGCATTTAAAGACTTGTTAGAAGAGTATAACGTTGAGTTGAAAGGGCCCTTCAATTATTCAGCTCGTGATGAAGCTGGCATTCCTCGTGATTGGTATGATTTATCTACAAATAACAAGGACATGAAAAGGGATAACAGTGAGAAGCTTTCTGAGGTGTATGAGAGGTTGGCTTCCATAATTTCCATGGAGAATGAGAACTCGAGTTTGAACAGGCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF455

Protein Analysis

369

Amino Acids

41.06

Weight (kDa)

6.49

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF455 PF04305 84 - 329 1.1e-85 Protein of unknown function (DUF455)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06240
fragaria_vesca FvH4_6g27080 FvH4_6g27080
malus_domestica MD09G1240300.v1.1
prunus_persica Prupe.3G124300_v2.0.a1
pyrus_communis pycom09g15890
rosa_chinensis RchiOBHm_Chr2g0129041
rosa_laevigata RLG00000019046
rosa_multiflora Rmu_co8451771.1_g000001
rosa_roxburghii Rroxscaffold_2G00114940
rosa_rugosa Rorug02G0283600 Rorug02G0283700
rosa_samantha Rh2AG336100 Rh2BG344100 Rh2CG323000 Rh2DG360700
rosa_wichuraiana Rw2G027250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 95
AciI CCGC 6 cut(s) 41, 210, 351, 355, 419, 571
AclI AACGTT 1 cut(s) 918
AcsI RAATTY 1 cut(s) 390
AcvI CACGTG 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 304
AfiI CCNNNNNNNGG 3 cut(s) 95, 142, 741
AflIII ACRYGT 1 cut(s) 823
AgsI TTSAA 4 cut(s) 616, 928, 940, 1096
AjnI CCWGG 1 cut(s) 711
AluBI AGCT 6 cut(s) 229, 460, 830, 950, 962, 1033
AluI AGCT 6 cut(s) 229, 460, 830, 950, 962, 1033
Alw21I GWGCWC 2 cut(s) 238, 462
Alw26I GTCTC 2 cut(s) 7, 104
AlwNI CAGNNNCTG 1 cut(s) 858
Ama87I CYCGRG 2 cut(s) 409, 1087
AoxI GGCC 4 cut(s) 154, 447, 717, 932
ApaI GGGCCC 2 cut(s) 158, 936
ApeKI GCWGC 3 cut(s) 4, 199, 683
ApoI RAATTY 1 cut(s) 390
AspLEI GCGC 2 cut(s) 306, 878
AspS9I GGNCC 8 cut(s) 146, 154, 155, 448, 709, 737, 932, 933
AsuHPI GGTGA 3 cut(s) 78, 158, 173
AvaI CYCGRG 2 cut(s) 409, 1087
AvaII GGWCC 3 cut(s) 146, 709, 737
BaeGI GKGCMC 2 cut(s) 158, 936
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 158, 462, 936
BauI CACGAG 2 cut(s) 951, 972
BbrPI CACGTG 1 cut(s) 300
Bbv12I GWGCWC 2 cut(s) 238, 462
BbvI GCAGC 3 cut(s) 16, 186, 670
BccI CCATC 1 cut(s) 889
BceAI ACGGC 2 cut(s) 187, 758
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 1 cut(s) 713
BcoDI GTCTC 2 cut(s) 7, 104
BfaI CTAG 2 cut(s) 521, 798
BfoI RGCGCY 1 cut(s) 307
BglII AGATCT 1 cut(s) 772
BisI GCNGC 5 cut(s) 5, 200, 419, 571, 684
BlsI GCNGC 5 cut(s) 6, 201, 420, 572, 685
Bme1390I CCNGG 1 cut(s) 713
Bme18I GGWCC 3 cut(s) 146, 709, 737
BmeT110I CYCGRG 2 cut(s) 409, 1087
BmgT120I GGNCC 8 cut(s) 146, 154, 155, 448, 709, 737, 932, 933
BmiI GGNNCC 7 cut(s) 147, 148, 156, 157, 305, 399, 934
BmrFI CCNGG 1 cut(s) 713
BmsI GCATC 2 cut(s) 388, 721
BpmI CTGGAG 2 cut(s) 18, 670
BsaAI YACGTR 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 304
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 4 cut(s) 89, 150, 740, 1071
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 643, 673
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 142, 741
Bse1I ACTGG 3 cut(s) 378, 732, 848
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 4 cut(s) 89, 150, 740, 1071
BseGI GGATG 3 cut(s) 206, 379, 736
BseLI CCNNNNNNNGG 3 cut(s) 95, 142, 741
BseMII CTCAG 3 cut(s) 605, 790, 1029
BseNI ACTGG 3 cut(s) 378, 732, 848
BseRI GAGGAG 1 cut(s) 333
BseSI GKGCMC 2 cut(s) 158, 936
BseXI GCAGC 3 cut(s) 16, 186, 670
BseYI CCCAGC 1 cut(s) 141
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 4 cut(s) 156, 449, 719, 934
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 2 cut(s) 238, 462
BsiHKCI CYCGRG 2 cut(s) 409, 1087
BsiSI CCGG 2 cut(s) 395, 735
BslFI GGGAC 4 cut(s) 159, 256, 333, 497
BslI CCNNNNNNNGG 3 cut(s) 95, 142, 741
BsmAI GTCTC 2 cut(s) 7, 104
BsmFI GGGAC 4 cut(s) 159, 256, 333, 497
BsmI GAATGC 2 cut(s) 430, 966
BsnI GGCC 4 cut(s) 156, 449, 719, 934
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 2 cut(s) 409, 1087
Bsp120I GGGCCC 2 cut(s) 154, 932
Bsp1286I GDGCHC 4 cut(s) 158, 238, 462, 936
Bsp143I GATC 1 cut(s) 772
Bsp19I CCATGG 2 cut(s) 89, 1071
BspACI CCGC 6 cut(s) 41, 210, 351, 355, 419, 571
BspANI GGCC 4 cut(s) 156, 449, 719, 934
BspCNI CTCAG 3 cut(s) 604, 789, 1030
BspLI GGNNCC 7 cut(s) 147, 148, 156, 157, 305, 399, 934
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 1 cut(s) 104
BsrI ACTGG 3 cut(s) 378, 732, 848
BssECI CCNNGG 4 cut(s) 89, 150, 740, 1071
BssMI GATC 1 cut(s) 772
BssNI GRCGYC 1 cut(s) 304
BssSI CACGAG 2 cut(s) 951, 972
BssT1I CCWWGG 3 cut(s) 89, 150, 1071
Bst2BI CACGAG 2 cut(s) 951, 972
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 3 cut(s) 137, 490, 1025
Bst6I CTCTTC 2 cut(s) 178, 903
BstACI GRCGYC 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 4 cut(s) 355, 600, 633, 964
BstDEI CTNAG 3 cut(s) 591, 776, 1038
BstDSI CCRYGG 3 cut(s) 89, 740, 1071
BstF5I GGATG 3 cut(s) 206, 379, 736
BstH2I RGCGCY 1 cut(s) 307
BstHHI GCGC 2 cut(s) 306, 878
BstKTI GATC 1 cut(s) 775
BstMAI GTCTC 2 cut(s) 7, 104
BstMBI GATC 1 cut(s) 772
BstMWI GCNNNNNNNGC 4 cut(s) 545, 554, 692, 803
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 2 cut(s) 28, 827
BstSCI CCNGG 1 cut(s) 711
BstSLI GKGCMC 2 cut(s) 158, 936
BstV1I GCAGC 3 cut(s) 16, 186, 670
BstX2I RGATCY 1 cut(s) 772
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 1 cut(s) 772
BsuRI GGCC 4 cut(s) 156, 449, 719, 934
BtgI CCRYGG 3 cut(s) 89, 740, 1071
BtsCI GGATG 3 cut(s) 206, 379, 736
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 5 cut(s) 43, 486, 841, 859, 1030
Cac8I GCNNGC 4 cut(s) 355, 600, 633, 964
CaiI CAGNNNCTG 1 cut(s) 858
CfoI GCGC 2 cut(s) 306, 878
Cfr13I GGNCC 8 cut(s) 146, 154, 155, 448, 709, 737, 932, 933
CseI GACGC 1 cut(s) 197
CviAII CATG 8 cut(s) 25, 90, 330, 452, 551, 824, 1009, 1072
DdeI CTNAG 3 cut(s) 591, 776, 1038
DinI GGCGCC 1 cut(s) 305
DpnI GATC 1 cut(s) 774
DpnII GATC 1 cut(s) 772
DraI TTTAAA 1 cut(s) 895
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 2 cut(s) 178, 903
EarI CTCTTC 2 cut(s) 178, 903
Ecl136II GAGCTC 1 cut(s) 460
Eco130I CCWWGG 3 cut(s) 89, 150, 1071
Eco24I GRGCYC 3 cut(s) 158, 462, 936
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 3 cut(s) 146, 709, 737
Eco53kI GAGCTC 1 cut(s) 460
Eco72I CACGTG 1 cut(s) 300
Eco88I CYCGRG 2 cut(s) 409, 1087
EcoICRI GAGCTC 1 cut(s) 460
EcoO109I RGGNCCY 5 cut(s) 146, 154, 155, 932, 933
EcoRII CCWGG 1 cut(s) 711
EcoT14I CCWWGG 3 cut(s) 89, 150, 1071
EcoT38I GRGCYC 3 cut(s) 158, 462, 936
EgeI GGCGCC 1 cut(s) 305
EheI GGCGCC 1 cut(s) 305
ErhI CCWWGG 3 cut(s) 89, 150, 1071
FaeI CATG 8 cut(s) 28, 93, 333, 455, 554, 827, 1012, 1075
FaqI GGGAC 4 cut(s) 159, 256, 333, 497
FatI CATG 8 cut(s) 24, 89, 329, 451, 550, 823, 1008, 1071
FauI CCCGC 2 cut(s) 358, 362
Fnu4HI GCNGC 5 cut(s) 5, 200, 419, 571, 684
FokI GGATG 3 cut(s) 193, 366, 743
FriOI GRGCYC 3 cut(s) 158, 462, 936
Fsp4HI GCNGC 5 cut(s) 5, 200, 419, 571, 684
FspBI CTAG 2 cut(s) 521, 798
GlaI GCGC 2 cut(s) 305, 877
GluI GCNGC 5 cut(s) 5, 200, 419, 571, 684
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 2 cut(s) 18, 670
HaeII RGCGCY 1 cut(s) 307
HaeIII GGCC 4 cut(s) 156, 449, 719, 934
HapII CCGG 2 cut(s) 395, 735
HgaI GACGC 1 cut(s) 197
HhaI GCGC 2 cut(s) 306, 878
Hin1I GRCGYC 1 cut(s) 304
Hin1II CATG 8 cut(s) 28, 93, 333, 455, 554, 827, 1012, 1075
Hin6I GCGC 2 cut(s) 304, 876
HinP1I GCGC 2 cut(s) 304, 876
HindIII AAGCTT 1 cut(s) 1031
HinfI GANTC 1 cut(s) 15
HpaII CCGG 2 cut(s) 395, 735
HphI GGTGA 3 cut(s) 78, 158, 173
Hpy166II GTNNAC 1 cut(s) 841
Hpy188I TCNGA 6 cut(s) 384, 594, 673, 787, 864, 1039
Hpy188III TCNNGA 6 cut(s) 101, 411, 649, 778, 953, 974
Hpy8I GTNNAC 1 cut(s) 841
Hpy99I CGWCG 1 cut(s) 563
HpyAV CCTTC 3 cut(s) 188, 521, 946
HpyCH4III ACNGT 3 cut(s) 137, 490, 1025
HpyCH4IV ACGT 3 cut(s) 299, 688, 918
HpyCH4V TGCA 5 cut(s) 4, 379, 686, 751, 890
HpyF10VI GCNNNNNNNGC 4 cut(s) 545, 554, 692, 803
HpyF3I CTNAG 3 cut(s) 591, 776, 1038
HpySE526I ACGT 3 cut(s) 299, 688, 918
Hsp92I GRCGYC 1 cut(s) 304
Hsp92II CATG 8 cut(s) 28, 93, 333, 455, 554, 827, 1012, 1075
HspAI GCGC 2 cut(s) 304, 876
KasI GGCGCC 1 cut(s) 303
KflI GGGWCCC 1 cut(s) 146
Kzo9I GATC 1 cut(s) 772
LmnI GCTCC 3 cut(s) 196, 403, 457
Lsp1109I GCAGC 3 cut(s) 16, 186, 670
LweI GCATC 2 cut(s) 388, 721
MaeI CTAG 2 cut(s) 521, 798
MaeII ACGT 3 cut(s) 299, 688, 918
MaeIII GTNAC 2 cut(s) 512, 817
MalI GATC 1 cut(s) 774
MboI GATC 1 cut(s) 772
MboII GAAGA 6 cut(s) 60, 63, 165, 824, 876, 920
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 4 cut(s) 158, 238, 462, 936
MluCI AATT 5 cut(s) 390, 755, 866, 940, 1065
Mly113I GGCGCC 1 cut(s) 304
MlyI GAGTC 1 cut(s) 9
MseI TTAA 2 cut(s) 363, 894
MspA1I CMGCKG 1 cut(s) 421
MspI CCGG 2 cut(s) 395, 735
MspR9I CCNGG 1 cut(s) 713
Mva1269I GAATGC 2 cut(s) 430, 966
MvaI CCWGG 1 cut(s) 713
MwoI GCNNNNNNNGC 4 cut(s) 545, 554, 692, 803
NarI GGCGCC 1 cut(s) 304
NcoI CCATGG 2 cut(s) 89, 1071
NdeII GATC 1 cut(s) 772
NlaIII CATG 8 cut(s) 28, 93, 333, 455, 554, 827, 1012, 1075
NlaIV GGNNCC 7 cut(s) 147, 148, 156, 157, 305, 399, 934
NmeAIII GCCGAG 1 cut(s) 250
NmuCI GTSAC 2 cut(s) 512, 817
NspI RCATGY 2 cut(s) 28, 827
PaeR7I CTCGAG 1 cut(s) 1087
PciI ACATGT 1 cut(s) 823
PctI GAATGC 2 cut(s) 430, 966
PflMI CCANNNNNTGG 1 cut(s) 95
PfoI TCCNGGA 1 cut(s) 711
PkrI GCNGC 5 cut(s) 6, 201, 420, 572, 685
PleI GAGTC 1 cut(s) 9
PluTI GGCGCC 1 cut(s) 307
PmaCI CACGTG 1 cut(s) 300
PmlI CACGTG 1 cut(s) 300
PpsI GAGTC 1 cut(s) 9
Ppu21I YACGTR 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 146
PscI ACATGT 1 cut(s) 823
Psp124BI GAGCTC 1 cut(s) 462
Psp1406I AACGTT 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 711
PspCI CACGTG 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 7 cut(s) 147, 148, 156, 157, 305, 399, 934
PspOMI GGGCCC 2 cut(s) 154, 932
PspPI GGNCC 8 cut(s) 146, 154, 155, 448, 709, 737, 932, 933
PspPPI RGGWCCY 1 cut(s) 146
PspXI VCTCGAGB 1 cut(s) 1087
PstNI CAGNNNCTG 1 cut(s) 858
PsuI RGATCY 1 cut(s) 772
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 363, 894
SatI GCNGC 5 cut(s) 5, 200, 419, 571, 684
Sau3AI GATC 1 cut(s) 772
Sau96I GGNCC 8 cut(s) 146, 154, 155, 448, 709, 737, 932, 933
SchI GAGTC 1 cut(s) 9
ScrFI CCNGG 1 cut(s) 713
SduI GDGCHC 4 cut(s) 158, 238, 462, 936
SfaNI GCATC 2 cut(s) 388, 721
SfoI GGCGCC 1 cut(s) 305
Sfr274I CTCGAG 1 cut(s) 1087
SinI GGWCC 3 cut(s) 146, 709, 737
SlaI CTCGAG 1 cut(s) 1087
SmlI CTYRAG 1 cut(s) 1087
SmoI CTYRAG 1 cut(s) 1087
Sse9I AATT 5 cut(s) 390, 755, 866, 940, 1065
SsiI CCGC 6 cut(s) 41, 210, 351, 355, 419, 571
SspDI GGCGCC 1 cut(s) 303
SspMI CTAG 2 cut(s) 521, 798
SstI GAGCTC 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 711
StyI CCWWGG 3 cut(s) 89, 150, 1071
TaaI ACNGT 3 cut(s) 137, 490, 1025
TaiI ACGT 3 cut(s) 302, 691, 921
TaqI TCGA 2 cut(s) 102, 1088
TasI AATT 5 cut(s) 390, 755, 866, 940, 1065
TauI GCSGC 2 cut(s) 421, 573
Tru1I TTAA 2 cut(s) 363, 894
Tru9I TTAA 2 cut(s) 363, 894
TscAI CASTG 5 cut(s) 50, 493, 848, 859, 1030
TseFI GTSAC 2 cut(s) 512, 817
TseI GCWGC 3 cut(s) 4, 199, 683
Tsp45I GTSAC 2 cut(s) 512, 817
TspDTI ATGAA 3 cut(s) 825, 972, 1025
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 5 cut(s) 50, 493, 848, 859, 1030
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 3 cut(s) 146, 709, 737
XapI RAATTY 1 cut(s) 390
XceI RCATGY 2 cut(s) 28, 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 1069
XhoI CTCGAG 1 cut(s) 1087
XspI CTAG 2 cut(s) 521, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.