MD09G1240300.v1.1

Protein of unknown function (DUF455)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0014019
Physical Location & Seq
Reverse (-)
30559457 .. 30563502
4046 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1240300.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGCAGCGTCTCAAAGTAAAGGCCATGCTTTCTCCCACCTTCCAAGCCTCCTCCTTTTCTTCTTCTTCTTCTTCTTGCTTGCAATACTCACCTTGGTCCGGCCTGCAAACCTGGAGAGAGTGCCCGCTCAATGAGAACCGTTGGTGGGGTACCAAGGGCCCACAGCCTCAGCCGCTACAGTCACAGCCCTCCTCCATTGACAAGCATTACAGTCAAATGGTGTCGTCAGCTTCCTCCCTCGCAGAGCTCGGCGCTCTGGTTCTTTCTACCAGCGACCCCCTCGCCAAGTCAAGGATTTCCCACCTCGCTTATTCCAGGTGGCGCCATGACCGCCTACCGCTCGGTGCATCTGATCCCCCTCCTCCGCCTGCTCGCCCCTTCAAACCCCAACTGGTTTCTCCTAAAGAAATTCCAGCTCCCAAGAACTCGGGTTTGCCTCTTAATGCTTATATGCTTCATAATCTTGCTCACGTTGAGCTCAATGCAATTGATTTGGCGTGGGATACTGTTGTTCGTTTTTCACCTTTTAGTGAGACTCTAGGGGAGGGTCTTTTTGCCGACTTTGCTCATGTCGCTGACGATGAGAGTCACCATTTTGCTTGGTGTTCTCAGAGGCTTGCTGAACTTGGTTTCAATTACGGAGATATGCCTGCTCATAATCTGCTTTGGAGGGAGTGTGAGAAATCATCTGACAATGTTGCTGCACGTTTGGCAGTGATCCCCTTAGTCCAGGAAGCTAGAGGACTAGATGCTGGACCTCGACTTGTACAAAAATTGGTTGGTTTTGGAGATCTTCGGACATCTAACATTGTTGCTAGAATCGCTGATGAAGAAGTTGCACATGTAGCTGTTGGTGTCCACTGGTTTGTCGATGTCTGTCAAAAAATGGGTTGTGCCCCATCTTCCGCATTTAAAGACTTGTTGAAAGAGTATAATGTGGAGTTGAGAGGGCCCTTCAATTATTCAGCTCGTGATGAAGCTGGCATTCCTCGTGATTGGTACGACTTATGCGCAAATAACCCGCATAAGGATAAGAAAACGGATAAAAGTGAGAAGCTTTCTGAGGTCTATGAAAGGCTTGCTTCCATAATTTCCATGGAGAGTGAGAACTCAAGTTTGAACAAGCCTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF455

Protein Analysis

378

Amino Acids

41.92

Weight (kDa)

6.19

Isoelectric Point (pI)

51.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF455 PF04305 90 - 335 2e-83 Protein of unknown function (DUF455)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06240
fragaria_vesca FvH4_6g27080 FvH4_6g27080
malus_domestica MD09G1240300.v1.1
prunus_persica Prupe.3G124300_v2.0.a1
pyrus_communis pycom09g15890
rosa_chinensis RchiOBHm_Chr2g0129041
rosa_laevigata RLG00000019046
rosa_multiflora Rmu_co8451771.1_g000001
rosa_roxburghii Rroxscaffold_2G00114940
rosa_rugosa Rorug02G0283600 Rorug02G0283700
rosa_samantha Rh2AG336100 Rh2BG344100 Rh2CG323000 Rh2DG360700
rosa_wichuraiana Rw2G027250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1012
Acc65I GGTACC 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 149, 321
AccBSI CCGCTC 2 cut(s) 127, 340
AciI CCGC 7 cut(s) 125, 173, 331, 338, 365, 906, 1022
AclWI GGATC 2 cut(s) 347, 712
AcsI RAATTY 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 322
AfaI GTAC 3 cut(s) 151, 768, 1001
AfiI CCNNNNNNNGG 4 cut(s) 98, 145, 291, 1027
AflIII ACRYGT 1 cut(s) 841
AgsI TTSAA 5 cut(s) 382, 634, 925, 958, 1120
AjnI CCWGG 3 cut(s) 110, 314, 729
AluBI AGCT 9 cut(s) 230, 247, 416, 478, 737, 848, 968, 980, 1057
AluI AGCT 9 cut(s) 230, 247, 416, 478, 737, 848, 968, 980, 1057
Alw21I GWGCWC 2 cut(s) 249, 480
Alw26I GTCTC 2 cut(s) 14, 527
AlwI GGATC 2 cut(s) 347, 712
Ama87I CYCGRG 1 cut(s) 427
AoxI GGCC 4 cut(s) 21, 100, 157, 950
ApaI GGGCCC 2 cut(s) 161, 954
ApeKI GCWGC 2 cut(s) 4, 701
ApoI RAATTY 1 cut(s) 408
Asp718I GGTACC 1 cut(s) 149
AspLEI GCGC 3 cut(s) 254, 324, 1013
AspS9I GGNCC 6 cut(s) 96, 157, 158, 755, 950, 951
AsuHPI GGTGA 3 cut(s) 81, 513, 581
AvaI CYCGRG 1 cut(s) 427
AvaII GGWCC 2 cut(s) 96, 755
BaeGI GKGCMC 4 cut(s) 125, 161, 898, 954
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BanI GGYRCC 2 cut(s) 149, 321
BanII GRGCYC 4 cut(s) 161, 249, 480, 954
BauI CACGAG 2 cut(s) 969, 990
Bbv12I GWGCWC 2 cut(s) 249, 480
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 16, 688
BccI CCATC 1 cut(s) 907
BciT130I CCWGG 3 cut(s) 112, 316, 731
BciVI GTATCC 1 cut(s) 496
BcoDI GTCTC 2 cut(s) 14, 527
BfaI CTAG 4 cut(s) 539, 738, 746, 816
BfmI CTRYAG 1 cut(s) 176
BfoI RGCGCY 2 cut(s) 255, 325
BfuI GTATCC 1 cut(s) 496
BglII AGATCT 1 cut(s) 790
BisI GCNGC 3 cut(s) 5, 173, 702
BlsI GCNGC 3 cut(s) 6, 174, 703
Bme1390I CCNGG 3 cut(s) 112, 316, 731
Bme18I GGWCC 2 cut(s) 96, 755
BmeT110I CYCGRG 1 cut(s) 427
BmgT120I GGNCC 6 cut(s) 96, 157, 158, 755, 950, 951
BmiI GGNNCC 4 cut(s) 151, 159, 323, 952
BmrFI CCNGG 3 cut(s) 112, 316, 731
BmsI GCATC 2 cut(s) 356, 739
BpmI CTGGAG 1 cut(s) 133
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 1096
BsaHI GRCGYC 1 cut(s) 322
BsaJI CCNNGG 3 cut(s) 92, 153, 1095
BsaXI ACNNNNNCTCC 2 cut(s) 661, 691
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 145, 291, 1027
Bse1I ACTGG 2 cut(s) 396, 866
BseBI CCWGG 3 cut(s) 112, 316, 731
BseDI CCNNGG 3 cut(s) 92, 153, 1095
BseLI CCNNNNNNNGG 4 cut(s) 98, 145, 291, 1027
BseMII CTCAG 4 cut(s) 182, 623, 1053, 1119
BseNI ACTGG 2 cut(s) 396, 866
BseRI GAGGAG 3 cut(s) 40, 181, 351
BseSI GKGCMC 4 cut(s) 125, 161, 898, 954
BseXI GCAGC 2 cut(s) 16, 688
BsgI GTGCAG 1 cut(s) 687
BshFI GGCC 4 cut(s) 23, 102, 159, 952
BshNI GGYRCC 2 cut(s) 149, 321
BsiHKAI GWGCWC 2 cut(s) 249, 480
BsiHKCI CYCGRG 1 cut(s) 427
BsiSI CCGG 1 cut(s) 99
BslI CCNNNNNNNGG 4 cut(s) 98, 145, 291, 1027
BsmAI GTCTC 2 cut(s) 14, 527
BsmBI CGTCTC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 984
BsnI GGCC 4 cut(s) 23, 102, 159, 952
BsoBI CYCGRG 1 cut(s) 427
Bsp120I GGGCCC 2 cut(s) 157, 950
Bsp1286I GDGCHC 6 cut(s) 125, 161, 249, 480, 898, 954
Bsp1407I TGTACA 1 cut(s) 766
Bsp143I GATC 3 cut(s) 352, 717, 790
Bsp19I CCATGG 1 cut(s) 1095
BspACI CCGC 7 cut(s) 125, 173, 331, 338, 365, 906, 1022
BspANI GGCC 4 cut(s) 23, 102, 159, 952
BspCNI CTCAG 4 cut(s) 181, 622, 1054, 1120
BspLI GGNNCC 4 cut(s) 151, 159, 323, 952
BspPI GGATC 2 cut(s) 347, 712
BspT107I GGYRCC 2 cut(s) 149, 321
BsrBI CCGCTC 2 cut(s) 127, 340
BsrGI TGTACA 1 cut(s) 766
BsrI ACTGG 2 cut(s) 396, 866
BssECI CCNNGG 3 cut(s) 92, 153, 1095
BssMI GATC 3 cut(s) 352, 717, 790
BssNI GRCGYC 1 cut(s) 322
BssSI CACGAG 2 cut(s) 969, 990
BssT1I CCWWGG 3 cut(s) 92, 153, 1095
Bst2BI CACGAG 2 cut(s) 969, 990
Bst2UI CCWGG 3 cut(s) 112, 316, 731
Bst4CI ACNGT 4 cut(s) 140, 180, 212, 508
BstACI GRCGYC 1 cut(s) 322
BstAUI TGTACA 1 cut(s) 766
BstC8I GCNNGC 9 cut(s) 80, 104, 125, 369, 373, 618, 651, 982, 1080
BstDEI CTNAG 5 cut(s) 168, 609, 724, 1062, 1128
BstDSI CCRYGG 1 cut(s) 1095
BstH2I RGCGCY 2 cut(s) 255, 325
BstHHI GCGC 3 cut(s) 254, 324, 1013
BstKTI GATC 3 cut(s) 355, 720, 793
BstMAI GTCTC 2 cut(s) 14, 527
BstMBI GATC 3 cut(s) 352, 717, 790
BstMWI GCNNNNNNNGC 7 cut(s) 172, 330, 563, 572, 710, 821, 845
BstNI CCWGG 3 cut(s) 112, 316, 731
BstNSI RCATGY 1 cut(s) 845
BstSCI CCNGG 3 cut(s) 110, 314, 729
BstSFI CTRYAG 1 cut(s) 176
BstSLI GKGCMC 4 cut(s) 125, 161, 898, 954
BstV1I GCAGC 2 cut(s) 16, 688
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 4 cut(s) 23, 102, 159, 952
BtgI CCRYGG 1 cut(s) 1095
BtsI GCAGTG 1 cut(s) 720
BtsIMutI CAGTG 2 cut(s) 720, 859
Cac8I GCNNGC 9 cut(s) 80, 104, 125, 369, 373, 618, 651, 982, 1080
CfoI GCGC 3 cut(s) 254, 324, 1013
Cfr13I GGNCC 6 cut(s) 96, 157, 158, 755, 950, 951
Csp6I GTAC 3 cut(s) 150, 767, 1000
CviAII CATG 5 cut(s) 25, 326, 569, 842, 1096
CviQI GTAC 3 cut(s) 150, 767, 1000
DdeI CTNAG 5 cut(s) 168, 609, 724, 1062, 1128
DinI GGCGCC 1 cut(s) 323
DpnI GATC 3 cut(s) 354, 719, 792
DpnII GATC 3 cut(s) 352, 717, 790
DraI TTTAAA 1 cut(s) 913
EciI GGCGGA 1 cut(s) 354
Ecl136II GAGCTC 2 cut(s) 247, 478
Eco130I CCWWGG 3 cut(s) 92, 153, 1095
Eco24I GRGCYC 4 cut(s) 161, 249, 480, 954
Eco47I GGWCC 2 cut(s) 96, 755
Eco53kI GAGCTC 2 cut(s) 247, 478
Eco88I CYCGRG 1 cut(s) 427
EcoICRI GAGCTC 2 cut(s) 247, 478
EcoO109I RGGNCCY 3 cut(s) 157, 950, 951
EcoRII CCWGG 3 cut(s) 110, 314, 729
EcoT14I CCWWGG 3 cut(s) 92, 153, 1095
EcoT38I GRGCYC 4 cut(s) 161, 249, 480, 954
EgeI GGCGCC 1 cut(s) 323
EheI GGCGCC 1 cut(s) 323
ErhI CCWWGG 3 cut(s) 92, 153, 1095
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 5 cut(s) 28, 329, 572, 845, 1099
FalI AAGNNNNNCTT 2 cut(s) 1066, 1098
FatI CATG 5 cut(s) 24, 325, 568, 841, 1095
FauI CCCGC 2 cut(s) 132, 1029
Fnu4HI GCNGC 3 cut(s) 5, 173, 702
FriOI GRGCYC 4 cut(s) 161, 249, 480, 954
Fsp4HI GCNGC 3 cut(s) 5, 173, 702
FspBI CTAG 4 cut(s) 539, 738, 746, 816
FspI TGCGCA 1 cut(s) 1012
GlaI GCGC 3 cut(s) 253, 323, 1012
GluI GCNGC 3 cut(s) 5, 173, 702
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 2 cut(s) 255, 325
HaeIII GGCC 4 cut(s) 23, 102, 159, 952
HapII CCGG 1 cut(s) 99
HhaI GCGC 3 cut(s) 254, 324, 1013
Hin1I GRCGYC 1 cut(s) 322
Hin1II CATG 5 cut(s) 28, 329, 572, 845, 1099
Hin6I GCGC 3 cut(s) 252, 322, 1011
HinP1I GCGC 3 cut(s) 252, 322, 1011
HindIII AAGCTT 1 cut(s) 1055
HinfI GANTC 3 cut(s) 535, 586, 819
HpaII CCGG 1 cut(s) 99
HphI GGTGA 3 cut(s) 81, 513, 581
Hpy166II GTNNAC 1 cut(s) 859
Hpy188I TCNGA 5 cut(s) 352, 612, 691, 798, 1063
Hpy188III TCNNGA 2 cut(s) 971, 992
Hpy8I GTNNAC 1 cut(s) 859
HpyAV CCTTC 3 cut(s) 49, 388, 964
HpyCH4III ACNGT 4 cut(s) 140, 180, 212, 508
HpyCH4IV ACGT 2 cut(s) 471, 706
HpyCH4V TGCA 7 cut(s) 4, 82, 106, 347, 485, 704, 839
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 330, 563, 572, 710, 821, 845
HpyF3I CTNAG 5 cut(s) 168, 609, 724, 1062, 1128
HpySE526I ACGT 2 cut(s) 471, 706
Hsp92I GRCGYC 1 cut(s) 322
Hsp92II CATG 5 cut(s) 28, 329, 572, 845, 1099
HspAI GCGC 3 cut(s) 252, 322, 1011
KasI GGCGCC 1 cut(s) 321
KpnI GGTACC 1 cut(s) 153
Kzo9I GATC 3 cut(s) 352, 717, 790
LmnI GCTCC 1 cut(s) 421
Lsp1109I GCAGC 2 cut(s) 16, 688
LweI GCATC 2 cut(s) 356, 739
MaeI CTAG 4 cut(s) 539, 738, 746, 816
MaeII ACGT 2 cut(s) 471, 706
MaeIII GTNAC 2 cut(s) 180, 587
MalI GATC 3 cut(s) 354, 719, 792
MbiI CCGCTC 2 cut(s) 127, 340
MboI GATC 3 cut(s) 352, 717, 790
MboII GAAGA 8 cut(s) 51, 54, 57, 60, 63, 785, 842, 894
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 6 cut(s) 125, 161, 249, 480, 898, 954
MluCI AATT 6 cut(s) 408, 486, 634, 773, 958, 1089
Mly113I GGCGCC 1 cut(s) 322
MlyI GAGTC 2 cut(s) 529, 595
MseI TTAA 2 cut(s) 441, 912
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 3 cut(s) 112, 316, 731
MunI CAATTG 1 cut(s) 486
Mva1269I GAATGC 1 cut(s) 984
MvaI CCWGG 3 cut(s) 112, 316, 731
MwoI GCNNNNNNNGC 7 cut(s) 172, 330, 563, 572, 710, 821, 845
NarI GGCGCC 1 cut(s) 322
NcoI CCATGG 1 cut(s) 1095
NdeII GATC 3 cut(s) 352, 717, 790
NlaIII CATG 5 cut(s) 28, 329, 572, 845, 1099
NlaIV GGNNCC 4 cut(s) 151, 159, 323, 952
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 2 cut(s) 180, 587
NsbI TGCGCA 1 cut(s) 1012
NspI RCATGY 1 cut(s) 845
PciI ACATGT 1 cut(s) 841
PctI GAATGC 1 cut(s) 984
PfeI GAWTC 1 cut(s) 819
PfoI TCCNGGA 1 cut(s) 729
PkrI GCNGC 3 cut(s) 6, 174, 703
PleI GAGTC 2 cut(s) 529, 594
PluTI GGCGCC 1 cut(s) 325
PpsI GAGTC 2 cut(s) 529, 594
PscI ACATGT 1 cut(s) 841
Psp124BI GAGCTC 2 cut(s) 249, 480
Psp6I CCWGG 3 cut(s) 110, 314, 729
PspGI CCWGG 3 cut(s) 110, 314, 729
PspN4I GGNNCC 4 cut(s) 151, 159, 323, 952
PspOMI GGGCCC 2 cut(s) 157, 950
PspPI GGNCC 6 cut(s) 96, 157, 158, 755, 950, 951
PsuI RGATCY 1 cut(s) 790
RsaI GTAC 3 cut(s) 151, 768, 1001
RsaNI GTAC 3 cut(s) 150, 767, 1000
SacI GAGCTC 2 cut(s) 249, 480
SaqAI TTAA 2 cut(s) 441, 912
SatI GCNGC 3 cut(s) 5, 173, 702
Sau3AI GATC 3 cut(s) 352, 717, 790
Sau96I GGNCC 6 cut(s) 96, 157, 158, 755, 950, 951
SchI GAGTC 2 cut(s) 529, 595
ScrFI CCNGG 3 cut(s) 112, 316, 731
SduI GDGCHC 6 cut(s) 125, 161, 249, 480, 898, 954
SfaNI GCATC 2 cut(s) 356, 739
SfcI CTRYAG 1 cut(s) 176
SfoI GGCGCC 1 cut(s) 323
SinI GGWCC 2 cut(s) 96, 755
SmlI CTYRAG 1 cut(s) 1111
SmoI CTYRAG 1 cut(s) 1111
Sse9I AATT 6 cut(s) 408, 486, 634, 773, 958, 1089
SsiI CCGC 7 cut(s) 125, 173, 331, 338, 365, 906, 1022
SspDI GGCGCC 1 cut(s) 321
SspMI CTAG 4 cut(s) 539, 738, 746, 816
SstI GAGCTC 2 cut(s) 249, 480
StyD4I CCNGG 3 cut(s) 110, 314, 729
StyI CCWWGG 3 cut(s) 92, 153, 1095
TaaI ACNGT 4 cut(s) 140, 180, 212, 508
TaiI ACGT 2 cut(s) 474, 709
TaqI TCGA 2 cut(s) 760, 870
TasI AATT 6 cut(s) 408, 486, 634, 773, 958, 1089
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 175
TfiI GAWTC 1 cut(s) 819
Tru1I TTAA 2 cut(s) 441, 912
Tru9I TTAA 2 cut(s) 441, 912
TscAI CASTG 2 cut(s) 720, 866
TseFI GTSAC 2 cut(s) 180, 587
TseI GCWGC 2 cut(s) 4, 701
Tsp45I GTSAC 2 cut(s) 180, 587
TspDTI ATGAA 4 cut(s) 446, 843, 990, 1086
TspGWI ACGGA 2 cut(s) 654, 1055
TspRI CASTG 2 cut(s) 720, 866
VpaK11BI GGWCC 2 cut(s) 96, 755
XapI RAATTY 1 cut(s) 408
XceI RCATGY 1 cut(s) 845
XcmI CCANNNNNNNNNTGG 1 cut(s) 1093
XspI CTAG 4 cut(s) 539, 738, 746, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.