Rw2G027250

Protein of unknown function (DUF455)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014019
Physical Location & Seq
Forward (+)
42593783 .. 42598873
5091 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027250.1

Sequence Viewer

Length: 1137 bp
ATGCAGCGTCTCAAACTCCACCACATGCTCTCTCCTCCATTGTGCTCCTCCCGTGCTCCTTTGTTATCATCATGCCTCAGAACCAAGTCTCTTTCCTCTTCTTGTATGCAACCCTCACCCTGGTCTGGTCTCGAAACCTGGAGGAATAGCCCCCTCAATGAGAACCGTTGCTGGGGACCCAAGGGCCCCCAAACAATTGACAACGCCGACACTCCACTGGTGTCGTCGGCTTCCTCTCTTGCAGAGCTTGGTGCTCTCGTTCTCTCCACAAGTGACCCCCTCGCCAAGTCCAACCTTTCCCACATCGCTTACTCCACGTGGCGCCAACACAACCTTCCACTTGGTGCATGCCAACCTCCTCCTCGTCCTGCCCGCCCTCTGAAACCCCAACTGGTCAGTTCATCCGAAAGAAATTCCAGCCCCAAGAACTCGGGTTTGCCTCTCAATGCGTATATGCTTCATAATCTTGCACATGTTGAGCTCAATGCGATTGATTTAGCATGGGATACTGTTGTCCGTTTCTCTCCTTTTAGTGAGATTCTAGGCGAAGGGTTTTTCGCTGACTTTGCTCACGTTGCCGACGATGAGAGTCGCCATTTTGCTTGGTGTTCTCAGAGGCTTGCCGAACTTGGTTTCAAGTATGGAGATATGCCTGCTCATAATCTGCTCTGGAGGGAGTGTGAGAAATCATCTGACAATGTTGCTGCACGTTTGGCTGTCATACCACTTGTCCAGGAGGCCAGAGGACTAGATGCTGGACCACGACTTGTGCAAAAATTGGTTGGCTTCGGAGATCTCAGGACATCTGAAATCGTAGCTAGAATTGCTAATGAAGAAGTGGCACATGTAGCTGTTGGTGTCCACTGGTTTGTCAGTGTCTGTCAGAAATTAGGTTGCGCACCGTCTTCTGCATTTAAAGACTTGTTAGAAGATTATAACGTTGAGTTGAAAGGGCCCTTCAATTATTCAGCTCGTGATGAAGCTGGCATTCCTCGTGATTGGTATGATTTATCTACAAATAACAAGGACAAGAACAAGAAAAGGGTAACAGTGAGAAGCTTTCTGAGGTATGTGTATGAGAGGCTTGCTTCCATAATTTCCATGGAGAGTGAGAACTCGAGTTTGAACAGGCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF455

Protein Analysis

378

Amino Acids

41.95

Weight (kDa)

8.05

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF455 PF04305 90 - 336 6.7e-84 Protein of unknown function (DUF455)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014019)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06240
fragaria_vesca FvH4_6g27080 FvH4_6g27080
malus_domestica MD09G1240300.v1.1
prunus_persica Prupe.3G124300_v2.0.a1
pyrus_communis pycom09g15890
rosa_chinensis RchiOBHm_Chr2g0129041
rosa_laevigata RLG00000019046
rosa_multiflora Rmu_co8451771.1_g000001
rosa_roxburghii Rroxscaffold_2G00114940
rosa_rugosa Rorug02G0283600 Rorug02G0283700
rosa_samantha Rh2AG336100 Rh2BG344100 Rh2CG323000 Rh2DG360700
rosa_wichuraiana Rw2G027250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 936
Acc16I TGCGCA 1 cut(s) 898
AccB1I GGYRCC 1 cut(s) 321
AciI CCGC 1 cut(s) 373
AclI AACGTT 1 cut(s) 939
AcsI RAATTY 1 cut(s) 412
AcvI CACGTG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 322
AdeI CACNNNGTG 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 120, 125, 172
AflIII ACRYGT 2 cut(s) 472, 844
AgsI TTSAA 4 cut(s) 637, 949, 961, 1126
AjnI CCWGG 3 cut(s) 119, 137, 732
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AluBI AGCT 7 cut(s) 247, 481, 818, 851, 971, 983, 1059
AluI AGCT 7 cut(s) 247, 481, 818, 851, 971, 983, 1059
Alw21I GWGCWC 4 cut(s) 47, 58, 256, 483
Alw26I GTCTC 3 cut(s) 14, 93, 134
AlwNI CAGNNNCTG 1 cut(s) 879
Ama87I CYCGRG 2 cut(s) 430, 1117
AoxI GGCC 4 cut(s) 184, 738, 953, 1130
ApaI GGGCCC 2 cut(s) 188, 957
ApeKI GCWGC 2 cut(s) 4, 704
ApoI RAATTY 1 cut(s) 412
AspLEI GCGC 2 cut(s) 324, 899
AspS9I GGNCC 6 cut(s) 176, 184, 185, 758, 953, 954
AsuHPI GGTGA 1 cut(s) 108
AvaI CYCGRG 2 cut(s) 430, 1117
AvaII GGWCC 2 cut(s) 176, 758
BaeGI GKGCMC 2 cut(s) 188, 957
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BanI GGYRCC 1 cut(s) 321
BanII GRGCYC 3 cut(s) 188, 483, 957
BauI CACGAG 2 cut(s) 972, 993
BbrPI CACGTG 1 cut(s) 318
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 4 cut(s) 47, 58, 256, 483
BbvI GCAGC 2 cut(s) 16, 691
BciT130I CCWGG 3 cut(s) 121, 139, 734
BciVI GTATCC 1 cut(s) 499
BcoDI GTCTC 3 cut(s) 14, 93, 134
BfaI CTAG 3 cut(s) 542, 749, 819
BfoI RGCGCY 1 cut(s) 325
BfuI GTATCC 1 cut(s) 499
BglII AGATCT 1 cut(s) 793
BisI GCNGC 2 cut(s) 5, 705
BlsI GCNGC 2 cut(s) 6, 706
Bme1390I CCNGG 3 cut(s) 121, 139, 734
Bme18I GGWCC 2 cut(s) 176, 758
BmeT110I CYCGRG 2 cut(s) 430, 1117
BmgT120I GGNCC 6 cut(s) 176, 184, 185, 758, 953, 954
BmiI GGNNCC 6 cut(s) 177, 178, 186, 187, 323, 955
BmrFI CCNGG 3 cut(s) 121, 139, 734
BmsI GCATC 1 cut(s) 742
BpiI GAAGAC 1 cut(s) 897
BpmI CTGGAG 2 cut(s) 160, 691
BsaAI YACGTR 1 cut(s) 318
BsaHI GRCGYC 1 cut(s) 322
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 3 cut(s) 119, 180, 1101
BsaXI ACNNNNNCTCC 2 cut(s) 664, 694
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 125, 172
Bse1I ACTGG 3 cut(s) 222, 396, 869
BseBI CCWGG 3 cut(s) 121, 139, 734
BseDI CCNNGG 3 cut(s) 119, 180, 1101
BseGI GGATG 1 cut(s) 401
BseLI CCNNNNNNNGG 3 cut(s) 120, 125, 172
BseMII CTCAG 4 cut(s) 91, 626, 811, 1055
BseNI ACTGG 3 cut(s) 222, 396, 869
BseRI GAGGAG 4 cut(s) 24, 37, 348, 351
BseSI GKGCMC 2 cut(s) 188, 957
BseXI GCAGC 2 cut(s) 16, 691
BseYI CCCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 4 cut(s) 186, 740, 955, 1132
BshNI GGYRCC 1 cut(s) 321
BsiHKAI GWGCWC 4 cut(s) 47, 58, 256, 483
BsiHKCI CYCGRG 2 cut(s) 430, 1117
BslFI GGGAC 1 cut(s) 189
BslI CCNNNNNNNGG 3 cut(s) 120, 125, 172
BsmAI GTCTC 3 cut(s) 14, 93, 134
BsmBI CGTCTC 1 cut(s) 14
BsmFI GGGAC 1 cut(s) 189
BsmI GAATGC 1 cut(s) 987
BsnI GGCC 4 cut(s) 186, 740, 955, 1132
Bso31I GGTCTC 1 cut(s) 134
BsoBI CYCGRG 2 cut(s) 430, 1117
Bsp120I GGGCCC 2 cut(s) 184, 953
Bsp1286I GDGCHC 6 cut(s) 47, 58, 188, 256, 483, 957
Bsp143I GATC 1 cut(s) 793
Bsp19I CCATGG 1 cut(s) 1101
BspACI CCGC 1 cut(s) 373
BspANI GGCC 4 cut(s) 186, 740, 955, 1132
BspCNI CTCAG 4 cut(s) 90, 625, 810, 1056
BspLI GGNNCC 6 cut(s) 177, 178, 186, 187, 323, 955
BspT107I GGYRCC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 134
BsrI ACTGG 3 cut(s) 222, 396, 869
BssECI CCNNGG 3 cut(s) 119, 180, 1101
BssMI GATC 1 cut(s) 793
BssNI GRCGYC 1 cut(s) 322
BssSI CACGAG 2 cut(s) 972, 993
BssT1I CCWWGG 2 cut(s) 180, 1101
Bst2BI CACGAG 2 cut(s) 972, 993
Bst2UI CCWGG 3 cut(s) 121, 139, 734
Bst4CI ACNGT 4 cut(s) 167, 511, 903, 1051
Bst6I CTCTTC 1 cut(s) 103
BstACI GRCGYC 1 cut(s) 322
BstBAI YACGTR 1 cut(s) 318
BstC8I GCNNGC 6 cut(s) 349, 373, 621, 654, 985, 1086
BstDEI CTNAG 5 cut(s) 77, 612, 797, 1064, 1134
BstDSI CCRYGG 1 cut(s) 1101
BstF5I GGATG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 325
BstHHI GCGC 2 cut(s) 324, 899
BstKTI GATC 1 cut(s) 796
BstMAI GTCTC 3 cut(s) 14, 93, 134
BstMBI GATC 1 cut(s) 793
BstMWI GCNNNNNNNGC 5 cut(s) 566, 575, 713, 824, 848
BstNI CCWGG 3 cut(s) 121, 139, 734
BstNSI RCATGY 4 cut(s) 28, 351, 476, 848
BstSCI CCNGG 3 cut(s) 119, 137, 732
BstSLI GKGCMC 2 cut(s) 188, 957
BstV1I GCAGC 2 cut(s) 16, 691
BstV2I GAAGAC 1 cut(s) 897
BstX2I RGATCY 1 cut(s) 793
BstYI RGATCY 1 cut(s) 793
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 4 cut(s) 186, 740, 955, 1132
BtgI CCRYGG 1 cut(s) 1101
BtgZI GCGATG 1 cut(s) 289
BtsCI GGATG 1 cut(s) 401
BtsIMutI CAGTG 4 cut(s) 215, 862, 880, 1056
Cac8I GCNNGC 6 cut(s) 349, 373, 621, 654, 985, 1086
CaiI CAGNNNCTG 1 cut(s) 879
CfoI GCGC 2 cut(s) 324, 899
Cfr13I GGNCC 6 cut(s) 176, 184, 185, 758, 953, 954
CviAII CATG 7 cut(s) 25, 72, 348, 473, 501, 845, 1102
DdeI CTNAG 5 cut(s) 77, 612, 797, 1064, 1134
DinI GGCGCC 1 cut(s) 323
DpnI GATC 1 cut(s) 795
DpnII GATC 1 cut(s) 793
DraI TTTAAA 1 cut(s) 916
DraIII CACNNNGTG 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 481
Eco130I CCWWGG 2 cut(s) 180, 1101
Eco147I AGGCCT 1 cut(s) 1132
Eco24I GRGCYC 3 cut(s) 188, 483, 957
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 2 cut(s) 176, 758
Eco53kI GAGCTC 1 cut(s) 481
Eco72I CACGTG 1 cut(s) 318
Eco88I CYCGRG 2 cut(s) 430, 1117
EcoICRI GAGCTC 1 cut(s) 481
EcoO109I RGGNCCY 5 cut(s) 176, 184, 185, 953, 954
EcoRII CCWGG 3 cut(s) 119, 137, 732
EcoT14I CCWWGG 2 cut(s) 180, 1101
EcoT38I GRGCYC 3 cut(s) 188, 483, 957
EgeI GGCGCC 1 cut(s) 323
EheI GGCGCC 1 cut(s) 323
ErhI CCWWGG 2 cut(s) 180, 1101
Esp3I CGTCTC 1 cut(s) 14
FaeI CATG 7 cut(s) 28, 75, 351, 476, 504, 848, 1105
FaqI GGGAC 1 cut(s) 189
FatI CATG 7 cut(s) 24, 71, 347, 472, 500, 844, 1101
FauI CCCGC 1 cut(s) 380
Fnu4HI GCNGC 2 cut(s) 5, 705
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 3 cut(s) 188, 483, 957
Fsp4HI GCNGC 2 cut(s) 5, 705
FspBI CTAG 3 cut(s) 542, 749, 819
FspI TGCGCA 1 cut(s) 898
GlaI GCGC 2 cut(s) 323, 898
GluI GCNGC 2 cut(s) 5, 705
GsaI CCCAGC 1 cut(s) 175
GsuI CTGGAG 2 cut(s) 160, 691
HaeII RGCGCY 1 cut(s) 325
HaeIII GGCC 4 cut(s) 186, 740, 955, 1132
HhaI GCGC 2 cut(s) 324, 899
Hin1I GRCGYC 1 cut(s) 322
Hin1II CATG 7 cut(s) 28, 75, 351, 476, 504, 848, 1105
Hin6I GCGC 2 cut(s) 322, 897
HinP1I GCGC 2 cut(s) 322, 897
HindIII AAGCTT 1 cut(s) 1057
HinfI GANTC 2 cut(s) 538, 589
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 1 cut(s) 862
Hpy188I TCNGA 9 cut(s) 80, 381, 406, 615, 694, 791, 808, 885, 1065
Hpy188III TCNNGA 5 cut(s) 131, 670, 799, 974, 995
Hpy8I GTNNAC 1 cut(s) 862
Hpy99I CGWCG 2 cut(s) 229, 584
HpyAV CCTTC 3 cut(s) 344, 542, 967
HpyCH4III ACNGT 4 cut(s) 167, 511, 903, 1051
HpyCH4IV ACGT 4 cut(s) 317, 573, 709, 939
HpyCH4V TGCA 8 cut(s) 4, 109, 242, 347, 470, 707, 772, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 566, 575, 713, 824, 848
HpyF3I CTNAG 5 cut(s) 77, 612, 797, 1064, 1134
HpySE526I ACGT 4 cut(s) 317, 573, 709, 939
Hsp92I GRCGYC 1 cut(s) 322
Hsp92II CATG 7 cut(s) 28, 75, 351, 476, 504, 848, 1105
HspAI GCGC 2 cut(s) 322, 897
KasI GGCGCC 1 cut(s) 321
KflI GGGWCCC 1 cut(s) 176
Kzo9I GATC 1 cut(s) 793
LmnI GCTCC 2 cut(s) 50, 61
Lsp1109I GCAGC 2 cut(s) 16, 691
LweI GCATC 1 cut(s) 742
MaeI CTAG 3 cut(s) 542, 749, 819
MaeII ACGT 4 cut(s) 317, 573, 709, 939
MaeIII GTNAC 2 cut(s) 272, 1045
MalI GATC 1 cut(s) 795
MboI GATC 1 cut(s) 793
MboII GAAGA 4 cut(s) 90, 845, 897, 941
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 1 cut(s) 793
MhlI GDGCHC 6 cut(s) 47, 58, 188, 256, 483, 957
MluCI AATT 7 cut(s) 195, 412, 776, 822, 887, 961, 1095
Mly113I GGCGCC 1 cut(s) 322
MlyI GAGTC 1 cut(s) 598
MmeI TCCRAC 1 cut(s) 315
MseI TTAA 1 cut(s) 915
MspR9I CCNGG 3 cut(s) 121, 139, 734
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 1 cut(s) 987
MvaI CCWGG 3 cut(s) 121, 139, 734
MwoI GCNNNNNNNGC 5 cut(s) 566, 575, 713, 824, 848
NarI GGCGCC 1 cut(s) 322
NcoI CCATGG 1 cut(s) 1101
NdeII GATC 1 cut(s) 793
NlaIII CATG 7 cut(s) 28, 75, 351, 476, 504, 848, 1105
NlaIV GGNNCC 6 cut(s) 177, 178, 186, 187, 323, 955
NmuCI GTSAC 1 cut(s) 272
NsbI TGCGCA 1 cut(s) 898
NspI RCATGY 4 cut(s) 28, 351, 476, 848
PaeI GCATGC 1 cut(s) 351
PaeR7I CTCGAG 1 cut(s) 1117
PceI AGGCCT 1 cut(s) 1132
PciI ACATGT 2 cut(s) 472, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 579
PctI GAATGC 1 cut(s) 987
PfeI GAWTC 1 cut(s) 538
PfoI TCCNGGA 1 cut(s) 732
PkrI GCNGC 2 cut(s) 6, 706
PleI GAGTC 1 cut(s) 597
PluTI GGCGCC 1 cut(s) 325
PmaCI CACGTG 1 cut(s) 318
PmlI CACGTG 1 cut(s) 318
PpsI GAGTC 1 cut(s) 597
Ppu21I YACGTR 1 cut(s) 318
PpuMI RGGWCCY 1 cut(s) 176
PscI ACATGT 2 cut(s) 472, 844
PsiI TTATAA 1 cut(s) 936
Psp124BI GAGCTC 1 cut(s) 483
Psp1406I AACGTT 1 cut(s) 939
Psp5II RGGWCCY 1 cut(s) 176
Psp6I CCWGG 3 cut(s) 119, 137, 732
PspCI CACGTG 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 3 cut(s) 119, 137, 732
PspN4I GGNNCC 6 cut(s) 177, 178, 186, 187, 323, 955
PspOMI GGGCCC 2 cut(s) 184, 953
PspPI GGNCC 6 cut(s) 176, 184, 185, 758, 953, 954
PspPPI RGGWCCY 1 cut(s) 176
PspXI VCTCGAGB 1 cut(s) 1117
PstNI CAGNNNCTG 1 cut(s) 879
PsuI RGATCY 1 cut(s) 793
SacI GAGCTC 1 cut(s) 483
SaqAI TTAA 1 cut(s) 915
SatI GCNGC 2 cut(s) 5, 705
Sau3AI GATC 1 cut(s) 793
Sau96I GGNCC 6 cut(s) 176, 184, 185, 758, 953, 954
SchI GAGTC 1 cut(s) 598
ScrFI CCNGG 3 cut(s) 121, 139, 734
SduI GDGCHC 6 cut(s) 47, 58, 188, 256, 483, 957
SfaNI GCATC 1 cut(s) 742
SfoI GGCGCC 1 cut(s) 323
Sfr274I CTCGAG 1 cut(s) 1117
SinI GGWCC 2 cut(s) 176, 758
SlaI CTCGAG 1 cut(s) 1117
SmlI CTYRAG 1 cut(s) 1117
SmoI CTYRAG 1 cut(s) 1117
SphI GCATGC 1 cut(s) 351
Sse9I AATT 7 cut(s) 195, 412, 776, 822, 887, 961, 1095
SseBI AGGCCT 1 cut(s) 1132
SsiI CCGC 1 cut(s) 373
SspDI GGCGCC 1 cut(s) 321
SspMI CTAG 3 cut(s) 542, 749, 819
SstI GAGCTC 1 cut(s) 483
StuI AGGCCT 1 cut(s) 1132
StyD4I CCNGG 3 cut(s) 119, 137, 732
StyI CCWWGG 2 cut(s) 180, 1101
TaaI ACNGT 4 cut(s) 167, 511, 903, 1051
TaiI ACGT 4 cut(s) 320, 576, 712, 942
TaqI TCGA 2 cut(s) 132, 1118
TasI AATT 7 cut(s) 195, 412, 776, 822, 887, 961, 1095
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 1 cut(s) 915
Tru9I TTAA 1 cut(s) 915
TscAI CASTG 4 cut(s) 222, 869, 880, 1056
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 2 cut(s) 4, 704
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 4 cut(s) 390, 449, 846, 993
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 4 cut(s) 222, 869, 880, 1056
VpaK11BI GGWCC 2 cut(s) 176, 758
XapI RAATTY 1 cut(s) 412
XceI RCATGY 4 cut(s) 28, 351, 476, 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XhoI CTCGAG 1 cut(s) 1117
XspI CTAG 3 cut(s) 542, 749, 819
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.