FvH4_6g29410

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013324
Physical Location & Seq
Reverse (-)
22690749 .. 22695292
4544 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29410.t2

Sequence Viewer

Length: 1155 bp
ATGGCTTGGGCTGCTCCTCCTCTGCTCTCTCTCCCTCTCAACCTCCTCCCACCCTCTTCTCCTCTCATCTCCAAATCCCACACGCCCAGCTGCCTCATAACCACTAGAAGATCATCGTTAAGAGTCACAGCCAGCGCTTCAGACCAAGACCAAACCAAGAACAAAGAAACAGAACAAGACTCGCCAGCCTTCAACCCATTTGGGTTCGTCACTGATAACCCCTCAAGCCGCACCGCCATTCAGCTCCCTGATACCCCTGCAGACGATGGCAATGTCGGCCAAATGCTTAACAGAATAGAAGAAAAGGGGAGGGATTATGGAAAATACATCAGGTCGGGGGAGCATGTATGGTTCGTGAGAGAAACTGGATCGGCTGAGAGCCAACGCGGAACAATTGTATTCCTTCATGGCGCTCCCACTCAGTCTTATAGCTACAGAAATGTCATGTCTCAGATGTCCGAACTTGGATTCCACTGCTTTGCACCTGATTGGCTAGGATTTGGTTTTAGTGACAAGCCTCAGCCAGGATACGATTTTGACTACACAGAAAAGGAGTTCCACGATGAATTTGACAGATTACTAGATGTGTTGGGGGTCACTTCTCCTTTCTATCTAGTTGTTCAGGGATTTCTTGTAGGTTCATATGGATTGACCTGGGCCATAAAAAATCCTAGCAGATTACAAAAGCTTGCAATTCTAAATAGTCCACTCACTGTGTCATCTCCTCTTCCAGGACTATTTAAACAACTAAGGTTTCCTCTACTTGGTGAGTTTACATGCCAGAATGCTATTATGGCTGAGCGCTTTATTGAAGCAGGTAGCGCTTACGCGTTGAAGCTGGAAAAGGCTGATGTGTATCGTTTACCATATCTATCAAGCAGTGGCCCTGGATTTGCTATACTTGAAGCTGCAAGGAAAGCTGATTTCCGAAGCATCCTGAGCCAAATAGCATCTGGCTTTGCATCTGGAAGATGGGATGTACCAACTCTTGTTGCATGGGGAATTTCAGACAAGTATCTACCTCAATCTGTGGCAGAGGAGTTTAAGAAAGCAAACCCCTCTGTTGTCAACCTTAAGTTGATAGAAGGTGCCGGGCATATGCCACAGGAGGACTGGCCTGAGAGAGTTGTTACTGCTCTAAGAGTGTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

385

Amino Acids

42.59

Weight (kDa)

5.93

Isoelectric Point (pI)

53.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 127 - 367 3.9e-11 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 131 - 371 1.4e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 132 - 378 3.2e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 806
AccB1I GGYRCC 1 cut(s) 1088
AccII CGCG 2 cut(s) 387, 830
AciI CCGC 3 cut(s) 229, 234, 387
AclWI GGATC 1 cut(s) 376
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 2 cut(s) 566, 1002
AcuI CTGAAG 1 cut(s) 123
AfaI GTAC 1 cut(s) 981
AfeI AGCGCT 3 cut(s) 136, 803, 823
AfiI CCNNNNNNNGG 3 cut(s) 524, 731, 764
AflII CTTAAG 1 cut(s) 1073
AflIII ACRYGT 1 cut(s) 828
AgsI TTSAA 4 cut(s) 193, 812, 835, 905
AjnI CCWGG 4 cut(s) 523, 653, 730, 886
AloI GAACNNNNNNTCC 2 cut(s) 453, 485
AluBI AGCT 7 cut(s) 90, 244, 432, 688, 838, 908, 920
AluI AGCT 7 cut(s) 90, 244, 432, 688, 838, 908, 920
Alw26I GTCTC 1 cut(s) 453
AlwI GGATC 1 cut(s) 376
Aor51HI AGCGCT 3 cut(s) 136, 803, 823
AoxI GGCC 4 cut(s) 277, 657, 883, 1115
ApeKI GCWGC 3 cut(s) 11, 90, 908
ApoI RAATTY 2 cut(s) 566, 1002
AspLEI GCGC 4 cut(s) 137, 413, 804, 824
AspS9I GGNCC 2 cut(s) 657, 884
AsuC2I CCSGG 1 cut(s) 1093
AsuHPI GGTGA 1 cut(s) 779
BanI GGYRCC 1 cut(s) 1088
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 2 cut(s) 77, 895
BccI CCATC 2 cut(s) 260, 966
BciT130I CCWGG 4 cut(s) 525, 655, 732, 888
BciVI GTATCC 1 cut(s) 521
BcnI CCSGG 1 cut(s) 1093
BcoDI GTCTC 1 cut(s) 453
BfaI CTAG 5 cut(s) 105, 494, 581, 614, 672
BfmI CTRYAG 2 cut(s) 258, 433
BfoI RGCGCY 4 cut(s) 138, 414, 805, 825
BfrI CTTAAG 1 cut(s) 1073
BfuAI ACCTGC 1 cut(s) 806
BfuI GTATCC 1 cut(s) 521
BisI GCNGC 4 cut(s) 12, 91, 229, 909
BlpI GCTNAGC 1 cut(s) 798
BlsI GCNGC 4 cut(s) 13, 92, 230, 910
Bme1390I CCNGG 5 cut(s) 525, 655, 732, 888, 1093
BmgT120I GGNCC 2 cut(s) 657, 884
BmiI GGNNCC 1 cut(s) 1090
BmrFI CCNGG 5 cut(s) 525, 655, 732, 888, 1093
BmsI GCATC 3 cut(s) 942, 959, 971
Bpu10I CCTNAGC 2 cut(s) 519, 938
Bpu1102I GCTNAGC 1 cut(s) 798
BpuEI CTTGAG 1 cut(s) 208
BpuMI CCSGG 1 cut(s) 1093
BsaBI GATNNNNATC 1 cut(s) 855
BsaJI CCNNGG 2 cut(s) 654, 886
Bsc4I CCNNNNNNNGG 3 cut(s) 524, 731, 764
Bse1I ACTGG 2 cut(s) 370, 1118
Bse3DI GCAATG 1 cut(s) 277
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 4 cut(s) 525, 655, 732, 888
BseDI CCNNGG 2 cut(s) 654, 886
BseGI GGATG 2 cut(s) 933, 982
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 3 cut(s) 524, 731, 764
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 7 cut(s) 366, 434, 464, 533, 789, 929, 1110
BseNI ACTGG 2 cut(s) 370, 1118
BseRI GAGGAG 6 cut(s) 6, 9, 35, 51, 714, 1052
BseXI GCAGC 2 cut(s) 77, 895
BseYI CCCAGC 1 cut(s) 86
Bsh1236I CGCG 2 cut(s) 387, 830
BshFI GGCC 4 cut(s) 279, 659, 885, 1117
BshNI GGYRCC 1 cut(s) 1088
BsiSI CCGG 1 cut(s) 1092
BslI CCNNNNNNNGG 3 cut(s) 524, 731, 764
BsmAI GTCTC 1 cut(s) 453
BsmI GAATGC 1 cut(s) 790
BsnI GGCC 4 cut(s) 279, 659, 885, 1117
Bsp143I GATC 2 cut(s) 110, 368
Bsp1720I GCTNAGC 1 cut(s) 798
BspACI CCGC 3 cut(s) 229, 234, 387
BspANI GGCC 4 cut(s) 279, 659, 885, 1117
BspCNI CTCAG 7 cut(s) 367, 433, 463, 532, 790, 930, 1111
BspFNI CGCG 2 cut(s) 387, 830
BspLI GGNNCC 1 cut(s) 1090
BspMAI CTGCAG 1 cut(s) 262
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 1 cut(s) 376
BspT107I GGYRCC 1 cut(s) 1088
BspTI CTTAAG 1 cut(s) 1073
BsrDI GCAATG 1 cut(s) 277
BsrI ACTGG 2 cut(s) 370, 1118
BssECI CCNNGG 2 cut(s) 654, 886
BssMI GATC 2 cut(s) 110, 368
Bst2UI CCWGG 4 cut(s) 525, 655, 732, 888
Bst4CI ACNGT 1 cut(s) 715
Bst6I CTCTTC 2 cut(s) 61, 732
BstAFI CTTAAG 1 cut(s) 1073
BstC8I GCNNGC 3 cut(s) 133, 186, 690
BstDEI CTNAG 9 cut(s) 375, 420, 450, 519, 749, 798, 938, 1119, 1139
BstENI CCTNNNNNAGG 2 cut(s) 522, 729
BstF5I GGATG 2 cut(s) 933, 982
BstFNI CGCG 2 cut(s) 387, 830
BstH2I RGCGCY 4 cut(s) 138, 414, 805, 825
BstHHI GCGC 4 cut(s) 137, 413, 804, 824
BstKTI GATC 2 cut(s) 113, 371
BstMAI GTCTC 1 cut(s) 453
BstMBI GATC 2 cut(s) 110, 368
BstMWI GCNNNNNNNGC 6 cut(s) 11, 276, 794, 821, 917, 939
BstNI CCWGG 4 cut(s) 525, 655, 732, 888
BstNSI RCATGY 2 cut(s) 347, 780
BstSCI CCNGG 5 cut(s) 523, 653, 730, 886, 1091
BstSFI CTRYAG 2 cut(s) 258, 433
BstUI CGCG 2 cut(s) 387, 830
BstV1I GCAGC 2 cut(s) 77, 895
BsuI GTATCC 1 cut(s) 521
BsuRI GGCC 4 cut(s) 279, 659, 885, 1117
BtsCI GGATG 2 cut(s) 933, 982
BtsI GCAGTG 2 cut(s) 472, 886
BtsIMutI CAGTG 4 cut(s) 210, 472, 711, 886
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 3 cut(s) 133, 186, 690
CfoI GCGC 4 cut(s) 137, 413, 804, 824
Cfr13I GGNCC 2 cut(s) 657, 884
Csp6I GTAC 1 cut(s) 980
CviAII CATG 5 cut(s) 344, 407, 445, 777, 996
CviQI GTAC 1 cut(s) 980
DdeI CTNAG 9 cut(s) 375, 420, 450, 519, 749, 798, 938, 1119, 1139
DpnI GATC 2 cut(s) 112, 370
DpnII GATC 2 cut(s) 110, 368
DraI TTTAAA 1 cut(s) 742
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 2 cut(s) 61, 732
EarI CTCTTC 2 cut(s) 61, 732
Eco47III AGCGCT 3 cut(s) 136, 803, 823
Eco57I CTGAAG 1 cut(s) 123
EcoNI CCTNNNNNAGG 2 cut(s) 522, 729
EcoRII CCWGG 4 cut(s) 523, 653, 730, 886
FaeI CATG 5 cut(s) 347, 410, 448, 780, 999
FatI CATG 5 cut(s) 343, 406, 444, 776, 995
FauNDI CATATG 2 cut(s) 643, 1098
Fnu4HI GCNGC 4 cut(s) 12, 91, 229, 909
FokI GGATG 2 cut(s) 920, 989
Fsp4HI GCNGC 4 cut(s) 12, 91, 229, 909
FspBI CTAG 5 cut(s) 105, 494, 581, 614, 672
GlaI GCGC 4 cut(s) 136, 412, 803, 823
GluI GCNGC 4 cut(s) 12, 91, 229, 909
GsaI CCCAGC 1 cut(s) 90
HaeII RGCGCY 4 cut(s) 138, 414, 805, 825
HaeIII GGCC 4 cut(s) 279, 659, 885, 1117
HapII CCGG 1 cut(s) 1092
HhaI GCGC 4 cut(s) 137, 413, 804, 824
Hin1II CATG 5 cut(s) 347, 410, 448, 780, 999
Hin6I GCGC 4 cut(s) 135, 411, 802, 822
HinP1I GCGC 4 cut(s) 135, 411, 802, 822
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 3 cut(s) 123, 179, 468
HpaII CCGG 1 cut(s) 1092
HphI GGTGA 1 cut(s) 779
Hpy166II GTNNAC 4 cut(s) 707, 774, 863, 1069
Hpy188I TCNGA 5 cut(s) 142, 453, 460, 929, 1009
Hpy188III TCNNGA 3 cut(s) 355, 937, 966
Hpy8I GTNNAC 4 cut(s) 707, 774, 863, 1069
HpyAV CCTTC 3 cut(s) 199, 413, 1079
HpyCH4III ACNGT 1 cut(s) 715
HpyCH4V TGCA 6 cut(s) 260, 482, 692, 911, 962, 995
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 276, 794, 821, 917, 939
HpyF3I CTNAG 9 cut(s) 375, 420, 450, 519, 749, 798, 938, 1119, 1139
Hsp92II CATG 5 cut(s) 347, 410, 448, 780, 999
HspAI GCGC 4 cut(s) 135, 411, 802, 822
Kzo9I GATC 2 cut(s) 110, 368
LmnI GCTCC 4 cut(s) 19, 249, 340, 418
Lsp1109I GCAGC 2 cut(s) 77, 895
LweI GCATC 3 cut(s) 942, 959, 971
MaeI CTAG 5 cut(s) 105, 494, 581, 614, 672
MaeIII GTNAC 5 cut(s) 124, 208, 509, 595, 1129
MalI GATC 2 cut(s) 112, 370
MboI GATC 2 cut(s) 110, 368
MboII GAAGA 5 cut(s) 48, 120, 311, 719, 981
MfeI CAATTG 1 cut(s) 393
MluCI AATT 4 cut(s) 393, 566, 693, 1002
MluI ACGCGT 1 cut(s) 828
MlyI GAGTC 2 cut(s) 132, 173
MseI TTAA 6 cut(s) 119, 288, 741, 1044, 1074, 1153
MspA1I CMGCKG 1 cut(s) 90
MspCI CTTAAG 1 cut(s) 1073
MspI CCGG 1 cut(s) 1092
MspR9I CCNGG 5 cut(s) 525, 655, 732, 888, 1093
MunI CAATTG 1 cut(s) 393
Mva1269I GAATGC 1 cut(s) 790
MvaI CCWGG 4 cut(s) 525, 655, 732, 888
MvnI CGCG 2 cut(s) 387, 830
MwoI GCNNNNNNNGC 6 cut(s) 11, 276, 794, 821, 917, 939
NciI CCSGG 1 cut(s) 1093
NdeI CATATG 2 cut(s) 643, 1098
NdeII GATC 2 cut(s) 110, 368
NlaIII CATG 5 cut(s) 347, 410, 448, 780, 999
NlaIV GGNNCC 1 cut(s) 1090
NmuCI GTSAC 4 cut(s) 124, 208, 509, 595
NspI RCATGY 2 cut(s) 347, 780
PctI GAATGC 1 cut(s) 790
PfeI GAWTC 1 cut(s) 468
PfoI TCCNGGA 1 cut(s) 730
PkrI GCNGC 4 cut(s) 13, 92, 230, 910
PleI GAGTC 2 cut(s) 131, 173
PpsI GAGTC 2 cut(s) 131, 173
Psp6I CCWGG 4 cut(s) 523, 653, 730, 886
PspFI CCCAGC 1 cut(s) 86
PspGI CCWGG 4 cut(s) 523, 653, 730, 886
PspN4I GGNNCC 1 cut(s) 1090
PspPI GGNCC 2 cut(s) 657, 884
PstI CTGCAG 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 90
RsaI GTAC 1 cut(s) 981
RsaNI GTAC 1 cut(s) 980
SaqAI TTAA 6 cut(s) 119, 288, 741, 1044, 1074, 1153
SatI GCNGC 4 cut(s) 12, 91, 229, 909
Sau3AI GATC 2 cut(s) 110, 368
Sau96I GGNCC 2 cut(s) 657, 884
SchI GAGTC 2 cut(s) 132, 173
ScrFI CCNGG 5 cut(s) 525, 655, 732, 888, 1093
SfaNI GCATC 3 cut(s) 942, 959, 971
SfcI CTRYAG 2 cut(s) 258, 433
SmlI CTYRAG 2 cut(s) 223, 1073
SmoI CTYRAG 2 cut(s) 223, 1073
Sse9I AATT 4 cut(s) 393, 566, 693, 1002
SsiI CCGC 3 cut(s) 229, 234, 387
SspMI CTAG 5 cut(s) 105, 494, 581, 614, 672
StyD4I CCNGG 5 cut(s) 523, 653, 730, 886, 1091
TaaI ACNGT 1 cut(s) 715
TasI AATT 4 cut(s) 393, 566, 693, 1002
TauI GCSGC 1 cut(s) 231
TfiI GAWTC 1 cut(s) 468
Tru1I TTAA 6 cut(s) 119, 288, 741, 1044, 1074, 1153
Tru9I TTAA 6 cut(s) 119, 288, 741, 1044, 1074, 1153
TscAI CASTG 4 cut(s) 217, 479, 718, 886
TseFI GTSAC 4 cut(s) 124, 208, 509, 595
TseI GCWGC 3 cut(s) 11, 90, 908
Tsp45I GTSAC 4 cut(s) 124, 208, 509, 595
TspDTI ATGAA 3 cut(s) 395, 579, 630
TspRI CASTG 4 cut(s) 217, 479, 718, 886
Vha464I CTTAAG 1 cut(s) 1073
XagI CCTNNNNNAGG 2 cut(s) 522, 729
XapI RAATTY 2 cut(s) 566, 1002
XceI RCATGY 2 cut(s) 347, 780
XcmI CCANNNNNNNNNTGG 2 cut(s) 950, 1110
XspI CTAG 5 cut(s) 105, 494, 581, 614, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.