Rorug02G0320600

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013324
Physical Location & Seq
Forward (+)
39230903 .. 39231656
754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0320600.1

Sequence Viewer

Length: 543 bp
ATGGCTGGCATTGTGAGGGTTGCCGTGCTGCCGGACCCAGAATCTGAGGCTCGATCTCGCTACCCTGTTTCGGGTGAGGCTGTGTCCAGAGATGGGTTTAATTTGGAGTATAAGTGGGAAGCAAAAGGGTCTGGGGATTTGCTTATGCTGGCTCATCCTCTACAAGTGAAGCTTCTTAAGAATGATGACTATGCATGGGACTCGTGGGTACTGAGGCCAGAGCCTGTTTCGGTTACTTGCCATTCGATTAAGGGTGTTAAGGAAAAGTCGAAAAGGAAGTTGGTTGCCAAGGCGGCGAGGCTGGCTTTGATTGCTGAGGAGGTGGGTTATGTTGATGTGATTCTGGCGATTGAGAAGTACTTGAGGGATGCAATTGAGCCATGGTTTTGGATGAGACTTTTGGTGGGAATGGGTTTTTGTATGAGAGTGAATGGGGTGGCATTGTTACTAAACAAGGATCATTGGATTCTGGTGCAGATGTCGGGTTTGGAGTTTACAATTGAAAGTACTAGAATGGCCGGGGGGGGTGAGGGTGGGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

180

Amino Acids

20.06

Weight (kDa)

6.84

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 293
AclWI GGATC 1 cut(s) 465
AcoI YGGCCR 1 cut(s) 516
AfaI GTAC 3 cut(s) 210, 359, 508
AfiI CCNNNNNNNGG 3 cut(s) 70, 71, 93
AflII CTTAAG 1 cut(s) 176
AgsI TTSAA 1 cut(s) 503
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 1 cut(s) 465
AlwNI CAGNNNCTG 2 cut(s) 44, 224
AoxI GGCC 2 cut(s) 215, 516
ApeKI GCWGC 1 cut(s) 28
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 520
AsuHPI GGTGA 2 cut(s) 86, 539
AvaII GGWCC 1 cut(s) 34
BauI CACGAG 1 cut(s) 202
BbvCI CCTCAGC 1 cut(s) 315
BbvI GCAGC 1 cut(s) 15
BccI CCATC 1 cut(s) 86
BceAI ACGGC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 183, 217
BcnI CCSGG 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 1 cut(s) 510
BfrI CTTAAG 1 cut(s) 176
BglI GCCNNNNNGGC 1 cut(s) 293
BisI GCNGC 2 cut(s) 29, 294
BlsI GCNGC 2 cut(s) 30, 295
BmcAI AGTACT 2 cut(s) 359, 508
Bme1390I CCNGG 1 cut(s) 520
Bme18I GGWCC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 520
BmsI GCATC 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 382
BpuMI CCSGG 1 cut(s) 520
BsaJI CCNNGG 3 cut(s) 288, 380, 519
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 71, 93
BseDI CCNNGG 3 cut(s) 288, 380, 519
BseGI GGATG 3 cut(s) 154, 373, 396
BseLI CCNNNNNNNGG 3 cut(s) 70, 71, 93
BseMII CTCAG 3 cut(s) 36, 203, 306
BseRI GAGGAG 1 cut(s) 332
BseXI GCAGC 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 2 cut(s) 217, 518
BsiSI CCGG 2 cut(s) 32, 519
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 70, 71, 93
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 217, 518
Bsp143I GATC 2 cut(s) 53, 457
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 1 cut(s) 293
BspANI GGCC 2 cut(s) 217, 518
BspCNI CTCAG 3 cut(s) 37, 204, 307
BspLI GGNNCC 1 cut(s) 36
BspPI GGATC 1 cut(s) 465
BspTI CTTAAG 1 cut(s) 176
BssECI CCNNGG 3 cut(s) 288, 380, 519
BssMI GATC 2 cut(s) 53, 457
BssSI CACGAG 1 cut(s) 202
BssT1I CCWWGG 2 cut(s) 288, 380
Bst2BI CACGAG 1 cut(s) 202
BstAFI CTTAAG 1 cut(s) 176
BstC8I GCNNGC 3 cut(s) 7, 150, 303
BstDEI CTNAG 3 cut(s) 45, 212, 315
BstDSI CCRYGG 1 cut(s) 380
BstF5I GGATG 3 cut(s) 154, 373, 396
BstKTI GATC 2 cut(s) 56, 460
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 2 cut(s) 53, 457
BstMWI GCNNNNNNNGC 3 cut(s) 293, 302, 311
BstSCI CCNGG 1 cut(s) 518
BstV1I GCAGC 1 cut(s) 15
BstXI CCANNNNNNTGG 1 cut(s) 387
BsuRI GGCC 2 cut(s) 217, 518
BtgI CCRYGG 1 cut(s) 380
BtsCI GGATG 3 cut(s) 154, 373, 396
Cac8I GCNNGC 3 cut(s) 7, 150, 303
CaiI CAGNNNCTG 2 cut(s) 44, 224
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 209, 358, 507
CviAII CATG 2 cut(s) 195, 381
CviQI GTAC 3 cut(s) 209, 358, 507
DdeI CTNAG 3 cut(s) 45, 212, 315
DpnI GATC 2 cut(s) 55, 459
DpnII GATC 2 cut(s) 53, 457
EaeI YGGCCR 1 cut(s) 516
Eco130I CCWWGG 2 cut(s) 288, 380
Eco47I GGWCC 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 288, 380
EcoT22I ATGCAT 1 cut(s) 196
ErhI CCWWGG 2 cut(s) 288, 380
FaeI CATG 2 cut(s) 198, 384
FaiI YATR 7 cut(s) 111, 146, 192, 196, 330, 382, 422
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 1 cut(s) 212
FatI CATG 2 cut(s) 194, 380
Fnu4HI GCNGC 2 cut(s) 29, 294
FokI GGATG 3 cut(s) 141, 380, 403
Fsp4HI GCNGC 2 cut(s) 29, 294
FspBI CTAG 1 cut(s) 510
GluI GCNGC 2 cut(s) 29, 294
HaeIII GGCC 2 cut(s) 217, 518
HapII CCGG 2 cut(s) 32, 519
Hin1II CATG 2 cut(s) 198, 384
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 4 cut(s) 41, 200, 340, 466
HpaII CCGG 2 cut(s) 32, 519
HphI GGTGA 2 cut(s) 86, 539
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 1 cut(s) 87
Hpy8I GTNNAC 1 cut(s) 495
HpyCH4V TGCA 3 cut(s) 194, 371, 475
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 302, 311
HpyF3I CTNAG 3 cut(s) 45, 212, 315
Hsp92II CATG 2 cut(s) 198, 384
Kzo9I GATC 2 cut(s) 53, 457
Lsp1109I GCAGC 1 cut(s) 15
LweI GCATC 1 cut(s) 358
MaeI CTAG 1 cut(s) 510
MaeIII GTNAC 2 cut(s) 232, 444
MalI GATC 2 cut(s) 55, 459
MboI GATC 2 cut(s) 53, 457
MfeI CAATTG 2 cut(s) 372, 498
MluCI AATT 3 cut(s) 100, 372, 498
MlyI GAGTC 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 196
MseI TTAA 4 cut(s) 99, 177, 249, 258
MspCI CTTAAG 1 cut(s) 176
MspI CCGG 2 cut(s) 32, 519
MspR9I CCNGG 1 cut(s) 520
MunI CAATTG 2 cut(s) 372, 498
MwoI GCNNNNNNNGC 3 cut(s) 293, 302, 311
NciI CCSGG 1 cut(s) 520
NcoI CCATGG 1 cut(s) 380
NdeII GATC 2 cut(s) 53, 457
NlaIII CATG 2 cut(s) 198, 384
NlaIV GGNNCC 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 196
PfeI GAWTC 3 cut(s) 41, 340, 466
PkrI GCNGC 2 cut(s) 30, 295
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 34
PstNI CAGNNNCTG 2 cut(s) 44, 224
RsaI GTAC 3 cut(s) 210, 359, 508
RsaNI GTAC 3 cut(s) 209, 358, 507
SaqAI TTAA 4 cut(s) 99, 177, 249, 258
SatI GCNGC 2 cut(s) 29, 294
Sau3AI GATC 2 cut(s) 53, 457
Sau96I GGNCC 1 cut(s) 34
ScaI AGTACT 2 cut(s) 359, 508
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 520
SetI ASST 2 cut(s) 174, 324
SfaNI GCATC 1 cut(s) 358
SinI GGWCC 1 cut(s) 34
SmlI CTYRAG 2 cut(s) 176, 361
SmoI CTYRAG 2 cut(s) 176, 361
Sse9I AATT 3 cut(s) 100, 372, 498
SsiI CCGC 1 cut(s) 293
SspMI CTAG 1 cut(s) 510
StyD4I CCNGG 1 cut(s) 518
StyI CCWWGG 2 cut(s) 288, 380
TaqI TCGA 3 cut(s) 52, 245, 269
TasI AATT 3 cut(s) 100, 372, 498
TatI WGTACW 2 cut(s) 357, 506
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 3 cut(s) 41, 340, 466
Tru1I TTAA 4 cut(s) 99, 177, 249, 258
Tru9I TTAA 4 cut(s) 99, 177, 249, 258
TseI GCWGC 1 cut(s) 28
Vha464I CTTAAG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 34
XspI CTAG 1 cut(s) 510
ZrmI AGTACT 2 cut(s) 359, 508
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.