Prupe.3G090600_v2.0.a1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0013324
Physical Location & Seq
Forward (+)
6721549 .. 6731646
10098 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G090600.1

Sequence Viewer

Length: 1170 bp
ATGGCTTGGGCTCTGCTCTCTCTGCCTCTCAACCTCCCTCCCTCTTCTTCTCCTCTGCCCTCCATACTCAATCCTTTCAAACCCAAATCCCAAATAGGCTGGCTTGCAAGTTGTAGTGGCAGTAGAAGAAGAGCAAGAGTCATAGCCAGTGCTGCAGACCAGGAAAAAACCGAGGACAAAGACGAAGACAAAGACTCGTCAGCTTTCAATCCCTTTGGCTTTGTCACTGATAACCCGTCAAGCCGCAGCGCCATTCAGATCATTGAGAGCCCTGCAGAGTCTGGCAATGTCGGCCAAATGCTTTATAGGATAGAAGACAAGGGGAAGGAATATGGAAAATACGTCAGATCAGGGGAGCATGTATGGTTTGTGAGAGAAACCGGATCAGCTGAGAGCCAACGTGGAACAGTTATCTTTCTTCATGGTGCTCCCACACAATCTTATAGCTACAGAAATGTTATGTCTCAGATGTCAGAACTTGGGTTCCACTGCTTTGCACCTGATTGGCTAGGATTTGGATTCAGTGACAAGCCACAGCCTGGATATGGATTTGATTACAAAGAAGAGGAGTTCCATGAAGAATTTGACAAATTACTAGATGTGCTTGGAGTCAAATCTCCTTTCTATCTGGTCGTTCAGGGATTTCTTGTAGGTTCATATGGATTGACATGGGCCTTAAAAAGCCGAAGCAGAATATCAAAGCTTGCAATTCTAAATAGTCCACTCACAGTTTCATCTCCTGTTCCTGGACTGTTCAAGCAACTAAGAATTCCTCTTTTTGGTGAATTTACATGCCAGAATGCTGTTATGGCAGAGCGCTTTATTGAAGCAGGTAGCGCTTACGTCTTGAAGCTGGAAAAGGCTGATGTGTACCGGCTACCATATCTTTCAAGCAGTGGACCTGGATTTGCTATACTTGAAGCTACAAGAAAGGCCAATTTCAATAATATATCAACCCAAATCGCATCTGGCTTTGCATCTGGAAGTTGGGATATACCGACTCTAGTTGCTTGGGGGATTGCAGACAAGTATCTACCTCAATCTGTGGCGGAAGAGTTTCAGAAAGGAAACCCTACTGCTGTCAAGCTTAAGTTGATAGAAGGTGCTGGGCATATGCCACAGGAGGACTGGCCAGAGAGAGTTGTTACTGCTCTGAGAGTATTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

390

Amino Acids

43.06

Weight (kDa)

6.27

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 821
AccB7I CCANNNNNTGG 1 cut(s) 539
AciI CCGC 2 cut(s) 244, 1049
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 2 cut(s) 292, 1130
AcsI RAATTY 3 cut(s) 581, 768, 785
AfaI GTAC 1 cut(s) 872
AfeI AGCGCT 2 cut(s) 818, 838
AfiI CCNNNNNNNGG 4 cut(s) 539, 545, 746, 779
AflII CTTAAG 1 cut(s) 1088
AgsI TTSAA 8 cut(s) 79, 208, 757, 827, 850, 891, 920, 943
AjnI CCWGG 4 cut(s) 159, 538, 745, 901
AloI GAACNNNNNNTCC 2 cut(s) 468, 500
AluBI AGCT 7 cut(s) 203, 389, 447, 703, 853, 923, 1087
AluI AGCT 7 cut(s) 203, 389, 447, 703, 853, 923, 1087
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 1 cut(s) 281
Aor51HI AGCGCT 2 cut(s) 818, 838
AoxI GGCC 4 cut(s) 292, 672, 933, 1130
ApeKI GCWGC 2 cut(s) 152, 246
ApoI RAATTY 3 cut(s) 581, 768, 785
Asp700I GAANNNNTTC 1 cut(s) 1056
AspLEI GCGC 3 cut(s) 251, 819, 839
AspS9I GGNCC 2 cut(s) 672, 899
AsuHPI GGTGA 1 cut(s) 794
AvaII GGWCC 1 cut(s) 899
BalI TGGCCA 1 cut(s) 1132
BanII GRGCYC 2 cut(s) 13, 272
BbsI GAAGAC 2 cut(s) 192, 321
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 2 cut(s) 139, 258
BciT130I CCWGG 4 cut(s) 161, 540, 747, 903
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 3 cut(s) 509, 596, 1004
BfmI CTRYAG 3 cut(s) 153, 273, 448
BfoI RGCGCY 3 cut(s) 252, 820, 840
BfrI CTTAAG 1 cut(s) 1088
BfuAI ACCTGC 1 cut(s) 821
BisI GCNGC 3 cut(s) 153, 244, 247
BlsI GCNGC 3 cut(s) 154, 245, 248
Bme1390I CCNGG 4 cut(s) 161, 540, 747, 903
Bme18I GGWCC 1 cut(s) 899
BmgT120I GGNCC 2 cut(s) 672, 899
BmiI GGNNCC 1 cut(s) 485
BmrFI CCNGG 4 cut(s) 161, 540, 747, 903
BmsI GCATC 2 cut(s) 974, 986
BpiI GAAGAC 2 cut(s) 192, 321
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 380
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 545, 746, 779
Bse118I RCCGGY 1 cut(s) 873
Bse1I ACTGG 2 cut(s) 147, 1133
Bse3DI GCAATG 1 cut(s) 292
BseBI CCWGG 4 cut(s) 161, 540, 747, 903
BseDI CCNNGG 1 cut(s) 171
BseLI CCNNNNNNNGG 4 cut(s) 539, 545, 746, 779
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 3 cut(s) 381, 479, 1145
BseNI ACTGG 2 cut(s) 147, 1133
BseRI GAGGAG 2 cut(s) 42, 581
BseXI GCAGC 2 cut(s) 139, 258
BseYI CCCAGC 1 cut(s) 1106
BshFI GGCC 4 cut(s) 294, 674, 935, 1132
BsiHKAI GWGCWC 1 cut(s) 430
BsiSI CCGG 2 cut(s) 381, 874
BslI CCNNNNNNNGG 4 cut(s) 539, 545, 746, 779
BsmAI GTCTC 1 cut(s) 468
BsmI GAATGC 1 cut(s) 805
BsnI GGCC 4 cut(s) 294, 674, 935, 1132
Bsp1286I GDGCHC 3 cut(s) 13, 272, 430
Bsp143I GATC 3 cut(s) 258, 347, 383
BspACI CCGC 2 cut(s) 244, 1049
BspANI GGCC 4 cut(s) 294, 674, 935, 1132
BspCNI CTCAG 3 cut(s) 382, 478, 1146
BspLI GGNNCC 1 cut(s) 485
BspMAI CTGCAG 2 cut(s) 157, 277
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 1 cut(s) 391
BspQI GCTCTTC 1 cut(s) 124
BspTI CTTAAG 1 cut(s) 1088
BsrDI GCAATG 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 873
BsrI ACTGG 2 cut(s) 147, 1133
BssAI RCCGGY 1 cut(s) 873
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 3 cut(s) 258, 347, 383
Bst2UI CCWGG 4 cut(s) 161, 540, 747, 903
Bst4CI ACNGT 3 cut(s) 409, 730, 753
Bst6I CTCTTC 4 cut(s) 49, 124, 558, 1047
BstAFI CTTAAG 1 cut(s) 1088
BstC8I GCNNGC 3 cut(s) 101, 105, 705
BstDEI CTNAG 4 cut(s) 390, 465, 764, 1154
BstH2I RGCGCY 3 cut(s) 252, 820, 840
BstHHI GCGC 3 cut(s) 251, 819, 839
BstKTI GATC 3 cut(s) 261, 350, 386
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 3 cut(s) 258, 347, 383
BstMWI GCNNNNNNNGC 5 cut(s) 22, 152, 291, 809, 836
BstNI CCWGG 4 cut(s) 161, 540, 747, 903
BstNSI RCATGY 2 cut(s) 362, 795
BstSCI CCNGG 4 cut(s) 159, 538, 745, 901
BstSFI CTRYAG 3 cut(s) 153, 273, 448
BstV1I GCAGC 2 cut(s) 139, 258
BstV2I GAAGAC 2 cut(s) 192, 321
BsuRI GGCC 4 cut(s) 294, 674, 935, 1132
BtsI GCAGTG 2 cut(s) 487, 901
BtsIMutI CAGTG 5 cut(s) 154, 225, 487, 529, 901
BveI ACCTGC 1 cut(s) 821
Cac8I GCNNGC 3 cut(s) 101, 105, 705
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 3 cut(s) 251, 819, 839
Cfr10I RCCGGY 1 cut(s) 873
Cfr13I GGNCC 2 cut(s) 672, 899
Csp6I GTAC 1 cut(s) 871
CviAII CATG 5 cut(s) 359, 422, 575, 669, 792
CviQI GTAC 1 cut(s) 871
DdeI CTNAG 4 cut(s) 390, 465, 764, 1154
DpnI GATC 3 cut(s) 260, 349, 385
DpnII GATC 3 cut(s) 258, 347, 383
EaeI YGGCCR 2 cut(s) 292, 1130
Eam1104I CTCTTC 4 cut(s) 49, 124, 558, 1047
EarI CTCTTC 4 cut(s) 49, 124, 558, 1047
EciI GGCGGA 1 cut(s) 1064
Eco24I GRGCYC 2 cut(s) 13, 272
Eco47I GGWCC 1 cut(s) 899
Eco47III AGCGCT 2 cut(s) 818, 838
EcoRI GAATTC 1 cut(s) 768
EcoRII CCWGG 4 cut(s) 159, 538, 745, 901
EcoT38I GRGCYC 2 cut(s) 13, 272
FaeI CATG 5 cut(s) 362, 425, 578, 672, 795
FatI CATG 5 cut(s) 358, 421, 574, 668, 791
FauNDI CATATG 2 cut(s) 658, 1113
Fnu4HI GCNGC 3 cut(s) 153, 244, 247
FriOI GRGCYC 2 cut(s) 13, 272
Fsp4HI GCNGC 3 cut(s) 153, 244, 247
FspBI CTAG 3 cut(s) 509, 596, 1004
GlaI GCGC 3 cut(s) 250, 818, 838
GluI GCNGC 3 cut(s) 153, 244, 247
GsaI CCCAGC 1 cut(s) 1110
HaeII RGCGCY 3 cut(s) 252, 820, 840
HaeIII GGCC 4 cut(s) 294, 674, 935, 1132
HapII CCGG 2 cut(s) 381, 874
HhaI GCGC 3 cut(s) 251, 819, 839
Hin1II CATG 5 cut(s) 362, 425, 578, 672, 795
Hin6I GCGC 3 cut(s) 249, 817, 837
HinP1I GCGC 3 cut(s) 249, 817, 837
HindIII AAGCTT 2 cut(s) 701, 1085
HinfI GANTC 6 cut(s) 138, 194, 278, 519, 609, 1000
HpaII CCGG 2 cut(s) 381, 874
HphI GGTGA 1 cut(s) 794
Hpy166II GTNNAC 3 cut(s) 722, 871, 899
Hpy188I TCNGA 6 cut(s) 258, 347, 468, 475, 1062, 1155
Hpy188III TCNNGA 2 cut(s) 847, 981
Hpy8I GTNNAC 3 cut(s) 722, 871, 899
HpyAV CCTTC 2 cut(s) 319, 1094
HpyCH4III ACNGT 3 cut(s) 409, 730, 753
HpyCH4IV ACGT 3 cut(s) 342, 400, 843
HpyCH4V TGCA 7 cut(s) 107, 155, 275, 497, 707, 977, 1022
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 152, 291, 809, 836
HpyF3I CTNAG 4 cut(s) 390, 465, 764, 1154
HpySE526I ACGT 3 cut(s) 342, 400, 843
Hsp92II CATG 5 cut(s) 362, 425, 578, 672, 795
HspAI GCGC 3 cut(s) 249, 817, 837
Kzo9I GATC 3 cut(s) 258, 347, 383
LguI GCTCTTC 1 cut(s) 124
LmnI GCTCC 2 cut(s) 355, 433
Lsp1109I GCAGC 2 cut(s) 139, 258
LweI GCATC 2 cut(s) 974, 986
MaeI CTAG 3 cut(s) 509, 596, 1004
MaeII ACGT 3 cut(s) 342, 400, 843
MaeIII GTNAC 3 cut(s) 223, 524, 1144
MalI GATC 3 cut(s) 260, 349, 385
MboI GATC 3 cut(s) 258, 347, 383
MhlI GDGCHC 3 cut(s) 13, 272, 430
MlsI TGGCCA 1 cut(s) 1132
MluCI AATT 6 cut(s) 581, 590, 708, 768, 785, 937
MluNI TGGCCA 1 cut(s) 1132
MlyI GAGTC 5 cut(s) 147, 188, 287, 618, 994
Mox20I TGGCCA 1 cut(s) 1132
MroXI GAANNNNTTC 1 cut(s) 1056
MscI TGGCCA 1 cut(s) 1132
MseI TTAA 3 cut(s) 677, 1089, 1168
Msp20I TGGCCA 1 cut(s) 1132
MspA1I CMGCKG 1 cut(s) 389
MspCI CTTAAG 1 cut(s) 1088
MspI CCGG 2 cut(s) 381, 874
MspR9I CCNGG 4 cut(s) 161, 540, 747, 903
Mva1269I GAATGC 1 cut(s) 805
MvaI CCWGG 4 cut(s) 161, 540, 747, 903
MwoI GCNNNNNNNGC 5 cut(s) 22, 152, 291, 809, 836
NdeI CATATG 2 cut(s) 658, 1113
NdeII GATC 3 cut(s) 258, 347, 383
NlaIII CATG 5 cut(s) 362, 425, 578, 672, 795
NlaIV GGNNCC 1 cut(s) 485
NmuCI GTSAC 2 cut(s) 223, 524
NspI RCATGY 2 cut(s) 362, 795
PciSI GCTCTTC 1 cut(s) 124
PctI GAATGC 1 cut(s) 805
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 1 cut(s) 519
PflMI CCANNNNNTGG 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 745
PkrI GCNGC 3 cut(s) 154, 245, 248
PleI GAGTC 5 cut(s) 146, 188, 286, 617, 994
PpsI GAGTC 5 cut(s) 146, 188, 286, 617, 994
Psp6I CCWGG 4 cut(s) 159, 538, 745, 901
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 4 cut(s) 159, 538, 745, 901
PspN4I GGNNCC 1 cut(s) 485
PspPI GGNCC 2 cut(s) 672, 899
PstI CTGCAG 2 cut(s) 157, 277
PstNI CAGNNNCTG 1 cut(s) 281
PvuII CAGCTG 1 cut(s) 389
RsaI GTAC 1 cut(s) 872
RsaNI GTAC 1 cut(s) 871
SapI GCTCTTC 1 cut(s) 124
SaqAI TTAA 3 cut(s) 677, 1089, 1168
SatI GCNGC 3 cut(s) 153, 244, 247
Sau3AI GATC 3 cut(s) 258, 347, 383
Sau96I GGNCC 2 cut(s) 672, 899
SchI GAGTC 5 cut(s) 147, 188, 287, 618, 994
ScrFI CCNGG 4 cut(s) 161, 540, 747, 903
SduI GDGCHC 3 cut(s) 13, 272, 430
SfaNI GCATC 2 cut(s) 974, 986
SfcI CTRYAG 3 cut(s) 153, 273, 448
SinI GGWCC 1 cut(s) 899
SmlI CTYRAG 1 cut(s) 1088
SmoI CTYRAG 1 cut(s) 1088
Sse9I AATT 6 cut(s) 581, 590, 708, 768, 785, 937
SsiI CCGC 2 cut(s) 244, 1049
SspMI CTAG 3 cut(s) 509, 596, 1004
StyD4I CCNGG 4 cut(s) 159, 538, 745, 901
TaaI ACNGT 3 cut(s) 409, 730, 753
TaiI ACGT 3 cut(s) 345, 403, 846
TasI AATT 6 cut(s) 581, 590, 708, 768, 785, 937
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 1 cut(s) 519
Tru1I TTAA 3 cut(s) 677, 1089, 1168
Tru9I TTAA 3 cut(s) 677, 1089, 1168
TscAI CASTG 5 cut(s) 154, 232, 494, 529, 901
TseFI GTSAC 2 cut(s) 223, 524
TseI GCWGC 2 cut(s) 152, 246
Tsp45I GTSAC 2 cut(s) 223, 524
TspDTI ATGAA 4 cut(s) 410, 591, 645, 723
TspRI CASTG 5 cut(s) 154, 232, 494, 529, 901
Van91I CCANNNNNTGG 1 cut(s) 539
Vha464I CTTAAG 1 cut(s) 1088
VpaK11BI GGWCC 1 cut(s) 899
XapI RAATTY 3 cut(s) 581, 768, 785
XceI RCATGY 2 cut(s) 362, 795
XcmI CCANNNNNNNNNTGG 2 cut(s) 965, 1125
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 3 cut(s) 509, 596, 1004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.