Rroxscaffold_2G00109700

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013324
Physical Location & Seq
Forward (+)
33883006 .. 33889264
6259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00109700.1

Sequence Viewer

Length: 1257 bp
ATGACACCATTTCGTGCCTTGTATGGTTATGAGCCACCAACAATACGAGGATACTCTGCGGGTTCTACAACTATAGAGCTCCAACAGCCACAACCAGAAAATGGCTTGGGCTCGTCCTCCTCTGCTTTCTCTCCCTCTCAAACTCCTCCCACCTCCTCTTCTCCTCTCAACTCCAACTCCCACACACGCAGCAGCTGGCTCATAACCACTAGAAAATCATCAAGAGTCACAGCCAGTGCTTCAGACCAAGACCAAACCAAAAACAATGAAAAACAACAAGACTCACCAGCTTTCAATCCCTTTGGTTTTGTCACTGATAACCCCTCAAGCCGTAGCGCTATTCAGCTCCCTGATAACCCTGCAGACGATGGCAATGTCGGCCAAATGCTTAATAGAATAGAAGACAAGGGGAGGGATTATGGAAAATACATCAGGTCAGGGGAGCATGTATGGTTCGTGAGAGAAACTGGATCAGCTGAGAGCCAACGTGGAACAATTGTCTTCCTTCACGGGGCTCCCACTCAGTCTTATAGCTACAGAAATGTTATGTCTCAGATGTCGGACCTTGGGTTCCACTGCTTTGCACCTGATTGGCTAGGATTTGGTTTCAGTGACAAACCACAGCCAGGATATGGATTTGACTACACAGAAAAGGAGTTCCACGACGAATTTGACAGATTACTAGATGTGCTGGGCGTCACATCTCCTTTCTATCTAGTCGTTCAGGGATTTCTTGTAGGTTCATATGGATTGACATGGGCAGTAAAAAATCCTAGCAGACTAGAGAAGCTTGCAATTCTAAATAGTCCACTCACCGTGTCATCTCCTCTTCCAGGACTGTTTAAGCAACTAAGATTTCCTCTACTTGGTGAGTTTACATGTCAGAATGCTATTATGGCTGAGCGCTTTATTGAAGCAGGTAGCGCTTATGCATTGAAGCTGGAAAAGGCTGATGTGTATCGGTTACCATATCTGTCAAGCAGTGGCCCTGGATTTGCTATACTTGAAGCTGCAAGGAAAGCTAATTTCCAAAGCATCCTGAGCCAAATAGCATCTGGCTTTGCATCTGGAAGATGGGATATACCGACTCTTGTTGCATGGGGAATTTCAGACAAGTATCTACCTCAATCTGTGGCAGAAGAGTTTAAGAAAGCAAACCCCAATGTTGTCAACCTTAAGTTGATAGAAGGTGCTGGGCATATGCCACAGGAGGACTGGCCTGAGAGAGTTGTTACTGCTCTGAGAGTGTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

418

Amino Acids

46.17

Weight (kDa)

5.94

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 161 - 401 2.6e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 165 - 405 2.5e-21 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 166 - 412 2e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 908
AccB7I CCANNNNNTGG 2 cut(s) 101, 632
AciI CCGC 1 cut(s) 59
AclWI GGATC 1 cut(s) 478
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 2 cut(s) 668, 1104
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 696
AfeI AGCGCT 3 cut(s) 337, 905, 925
AfiI CCNNNNNNNGG 6 cut(s) 101, 511, 626, 632, 833, 866
AflII CTTAAG 1 cut(s) 1175
AflIII ACRYGT 1 cut(s) 878
AgsI TTSAA 4 cut(s) 295, 914, 937, 1007
AjnI CCWGG 3 cut(s) 625, 832, 988
AloI GAACNNNNNNTCC 2 cut(s) 554, 586
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 555
AlwI GGATC 1 cut(s) 478
AlwNI CAGNNNCTG 1 cut(s) 195
Aor51HI AGCGCT 3 cut(s) 337, 905, 925
AoxI GGCC 3 cut(s) 379, 985, 1217
ApeKI GCWGC 3 cut(s) 189, 192, 1010
ApoI RAATTY 2 cut(s) 668, 1104
AspLEI GCGC 3 cut(s) 338, 906, 926
AspS9I GGNCC 2 cut(s) 562, 986
AsuHPI GGTGA 3 cut(s) 276, 805, 881
AvaII GGWCC 1 cut(s) 562
BanII GRGCYC 3 cut(s) 81, 113, 517
BbsI GAAGAC 2 cut(s) 408, 493
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 3 cut(s) 201, 204, 997
BccI CCATC 2 cut(s) 362, 1068
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 3 cut(s) 627, 834, 990
BciVI GTATCC 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 555
BfaI CTAG 6 cut(s) 210, 596, 683, 716, 774, 782
BfmI CTRYAG 3 cut(s) 72, 360, 535
BfoI RGCGCY 3 cut(s) 339, 907, 927
BfrI CTTAAG 1 cut(s) 1175
BfuAI ACCTGC 1 cut(s) 908
BfuI GTATCC 1 cut(s) 44
BisI GCNGC 3 cut(s) 190, 193, 1011
BlpI GCTNAGC 1 cut(s) 900
BlsI GCNGC 3 cut(s) 191, 194, 1012
Bme1390I CCNGG 3 cut(s) 627, 834, 990
Bme18I GGWCC 1 cut(s) 562
BmgT120I GGNCC 2 cut(s) 562, 986
BmiI GGNNCC 2 cut(s) 516, 572
BmrFI CCNGG 3 cut(s) 627, 834, 990
BmsI GCATC 3 cut(s) 1044, 1061, 1073
BpiI GAAGAC 2 cut(s) 408, 493
Bpu10I CCTNAGC 1 cut(s) 1040
Bpu1102I GCTNAGC 1 cut(s) 900
BpuEI CTTGAG 1 cut(s) 310
BsaBI GATNNNNATC 1 cut(s) 957
BsaHI GRCGYC 1 cut(s) 696
BsaJI CCNNGG 2 cut(s) 565, 988
BsaXI ACNNNNNCTCC 2 cut(s) 161, 191
Bsc4I CCNNNNNNNGG 6 cut(s) 101, 511, 626, 632, 833, 866
Bse1I ACTGG 3 cut(s) 234, 472, 1220
Bse3DI GCAATG 1 cut(s) 379
Bse8I GATNNNNATC 1 cut(s) 957
BseBI CCWGG 3 cut(s) 627, 834, 990
BseDI CCNNGG 2 cut(s) 565, 988
BseGI GGATG 1 cut(s) 1035
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 6 cut(s) 101, 511, 626, 632, 833, 866
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 7 cut(s) 468, 536, 566, 891, 1031, 1212, 1232
BseNI ACTGG 3 cut(s) 234, 472, 1220
BseRI GAGGAG 5 cut(s) 109, 135, 145, 153, 816
BseXI GCAGC 3 cut(s) 201, 204, 997
BseYI CCCAGC 2 cut(s) 691, 1193
BshFI GGCC 3 cut(s) 381, 987, 1219
BsiHKAI GWGCWC 1 cut(s) 81
BslI CCNNNNNNNGG 6 cut(s) 101, 511, 626, 632, 833, 866
BsmAI GTCTC 1 cut(s) 555
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 3 cut(s) 381, 987, 1219
Bsp1286I GDGCHC 3 cut(s) 81, 113, 517
Bsp143I GATC 1 cut(s) 470
Bsp1720I GCTNAGC 1 cut(s) 900
BspACI CCGC 1 cut(s) 59
BspANI GGCC 3 cut(s) 381, 987, 1219
BspCNI CTCAG 7 cut(s) 469, 535, 565, 892, 1032, 1213, 1233
BspLI GGNNCC 2 cut(s) 516, 572
BspMAI CTGCAG 1 cut(s) 364
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 1 cut(s) 478
BspTI CTTAAG 1 cut(s) 1175
BsrDI GCAATG 1 cut(s) 379
BsrI ACTGG 3 cut(s) 234, 472, 1220
BssECI CCNNGG 2 cut(s) 565, 988
BssMI GATC 1 cut(s) 470
BssNI GRCGYC 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 565
Bst2UI CCWGG 3 cut(s) 627, 834, 990
Bst4CI ACNGT 2 cut(s) 817, 840
Bst6I CTCTTC 3 cut(s) 163, 834, 1134
BstACI GRCGYC 1 cut(s) 696
BstAFI CTTAAG 1 cut(s) 1175
BstC8I GCNNGC 2 cut(s) 197, 792
BstDEI CTNAG 8 cut(s) 477, 522, 552, 851, 900, 1040, 1221, 1241
BstEII GGTNACC 1 cut(s) 963
BstENI CCTNNNNNAGG 1 cut(s) 831
BstF5I GGATG 1 cut(s) 1035
BstH2I RGCGCY 3 cut(s) 339, 907, 927
BstHHI GCGC 3 cut(s) 338, 906, 926
BstKTI GATC 1 cut(s) 473
BstMAI GTCTC 1 cut(s) 555
BstMBI GATC 1 cut(s) 470
BstMWI GCNNNNNNNGC 6 cut(s) 85, 378, 896, 923, 1019, 1041
BstNI CCWGG 3 cut(s) 627, 834, 990
BstNSI RCATGY 2 cut(s) 449, 882
BstPI GGTNACC 1 cut(s) 963
BstSCI CCNGG 3 cut(s) 625, 832, 988
BstSFI CTRYAG 3 cut(s) 72, 360, 535
BstV1I GCAGC 3 cut(s) 201, 204, 997
BstV2I GAAGAC 2 cut(s) 408, 493
BsuI GTATCC 1 cut(s) 44
BsuRI GGCC 3 cut(s) 381, 987, 1219
BtsCI GGATG 1 cut(s) 1035
BtsI GCAGTG 2 cut(s) 574, 988
BtsIMutI CAGTG 5 cut(s) 241, 312, 574, 616, 988
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 2 cut(s) 197, 792
CaiI CAGNNNCTG 1 cut(s) 195
CfoI GCGC 3 cut(s) 338, 906, 926
Cfr13I GGNCC 2 cut(s) 562, 986
CseI GACGC 1 cut(s) 685
CviAII CATG 4 cut(s) 446, 756, 879, 1098
DdeI CTNAG 8 cut(s) 477, 522, 552, 851, 900, 1040, 1221, 1241
DpnI GATC 1 cut(s) 472
DpnII GATC 1 cut(s) 470
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 3 cut(s) 163, 834, 1134
EarI CTCTTC 3 cut(s) 163, 834, 1134
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 565
Eco24I GRGCYC 3 cut(s) 81, 113, 517
Eco47I GGWCC 1 cut(s) 562
Eco47III AGCGCT 3 cut(s) 337, 905, 925
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 963
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 831
EcoO65I GGTNACC 1 cut(s) 963
EcoRII CCWGG 3 cut(s) 625, 832, 988
EcoT14I CCWWGG 1 cut(s) 565
EcoT22I ATGCAT 1 cut(s) 934
EcoT38I GRGCYC 3 cut(s) 81, 113, 517
ErhI CCWWGG 1 cut(s) 565
FaeI CATG 4 cut(s) 449, 759, 882, 1101
FatI CATG 4 cut(s) 445, 755, 878, 1097
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 2 cut(s) 745, 1200
Fnu4HI GCNGC 3 cut(s) 190, 193, 1011
FokI GGATG 1 cut(s) 1022
FriOI GRGCYC 3 cut(s) 81, 113, 517
Fsp4HI GCNGC 3 cut(s) 190, 193, 1011
FspBI CTAG 6 cut(s) 210, 596, 683, 716, 774, 782
GlaI GCGC 3 cut(s) 337, 905, 925
GluI GCNGC 3 cut(s) 190, 193, 1011
GsaI CCCAGC 2 cut(s) 695, 1197
HaeII RGCGCY 3 cut(s) 339, 907, 927
HaeIII GGCC 3 cut(s) 381, 987, 1219
HgaI GACGC 1 cut(s) 685
HhaI GCGC 3 cut(s) 338, 906, 926
Hin1I GRCGYC 1 cut(s) 696
Hin1II CATG 4 cut(s) 449, 759, 882, 1101
Hin6I GCGC 3 cut(s) 336, 904, 924
HinP1I GCGC 3 cut(s) 336, 904, 924
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 3 cut(s) 225, 281, 1087
HphI GGTGA 3 cut(s) 276, 805, 881
Hpy166II GTNNAC 3 cut(s) 809, 876, 1171
Hpy188I TCNGA 6 cut(s) 244, 555, 562, 885, 1111, 1242
Hpy188III TCNNGA 4 cut(s) 222, 457, 1039, 1068
Hpy8I GTNNAC 3 cut(s) 809, 876, 1171
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 2 cut(s) 515, 1181
HpyCH4III ACNGT 2 cut(s) 817, 840
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 7 cut(s) 362, 584, 794, 932, 1013, 1064, 1097
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 378, 896, 923, 1019, 1041
HpyF3I CTNAG 8 cut(s) 477, 522, 552, 851, 900, 1040, 1221, 1241
HpySE526I ACGT 1 cut(s) 487
Hsp92I GRCGYC 1 cut(s) 696
Hsp92II CATG 4 cut(s) 449, 759, 882, 1101
HspAI GCGC 3 cut(s) 336, 904, 924
Kzo9I GATC 1 cut(s) 470
LmnI GCTCC 4 cut(s) 84, 351, 442, 520
Lsp1109I GCAGC 3 cut(s) 201, 204, 997
LweI GCATC 3 cut(s) 1044, 1061, 1073
MaeI CTAG 6 cut(s) 210, 596, 683, 716, 774, 782
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 6 cut(s) 226, 310, 611, 697, 963, 1231
MalI GATC 1 cut(s) 472
MboI GATC 1 cut(s) 470
MboII GAAGA 6 cut(s) 150, 413, 493, 821, 1083, 1151
MfeI CAATTG 1 cut(s) 495
MhlI GDGCHC 3 cut(s) 81, 113, 517
MluCI AATT 5 cut(s) 495, 668, 795, 1024, 1104
MlyI GAGTC 3 cut(s) 234, 275, 1081
MmeI TCCRAC 3 cut(s) 106, 198, 540
Mph1103I ATGCAT 1 cut(s) 934
MseI TTAA 5 cut(s) 390, 843, 1146, 1176, 1255
MspA1I CMGCKG 2 cut(s) 195, 476
MspCI CTTAAG 1 cut(s) 1175
MspR9I CCNGG 3 cut(s) 627, 834, 990
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 3 cut(s) 627, 834, 990
MwoI GCNNNNNNNGC 6 cut(s) 85, 378, 896, 923, 1019, 1041
NdeI CATATG 2 cut(s) 745, 1200
NdeII GATC 1 cut(s) 470
NlaIII CATG 4 cut(s) 449, 759, 882, 1101
NlaIV GGNNCC 2 cut(s) 516, 572
NmuCI GTSAC 4 cut(s) 226, 310, 611, 697
NsiI ATGCAT 1 cut(s) 934
NspI RCATGY 2 cut(s) 449, 882
PciI ACATGT 1 cut(s) 878
PctI GAATGC 1 cut(s) 892
PflMI CCANNNNNTGG 2 cut(s) 101, 632
PfoI TCCNGGA 1 cut(s) 832
PkrI GCNGC 3 cut(s) 191, 194, 1012
PleI GAGTC 3 cut(s) 233, 275, 1081
PpsI GAGTC 3 cut(s) 233, 275, 1081
PscI ACATGT 1 cut(s) 878
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 3 cut(s) 625, 832, 988
PspEI GGTNACC 1 cut(s) 963
PspFI CCCAGC 2 cut(s) 691, 1193
PspGI CCWGG 3 cut(s) 625, 832, 988
PspN4I GGNNCC 2 cut(s) 516, 572
PspPI GGNCC 2 cut(s) 562, 986
PstI CTGCAG 1 cut(s) 364
PstNI CAGNNNCTG 1 cut(s) 195
PvuII CAGCTG 2 cut(s) 195, 476
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 5 cut(s) 390, 843, 1146, 1176, 1255
SatI GCNGC 3 cut(s) 190, 193, 1011
Sau3AI GATC 1 cut(s) 470
Sau96I GGNCC 2 cut(s) 562, 986
SchI GAGTC 3 cut(s) 234, 275, 1081
ScrFI CCNGG 3 cut(s) 627, 834, 990
SduI GDGCHC 3 cut(s) 81, 113, 517
SfaNI GCATC 3 cut(s) 1044, 1061, 1073
SfcI CTRYAG 3 cut(s) 72, 360, 535
SinI GGWCC 1 cut(s) 562
SmlI CTYRAG 2 cut(s) 325, 1175
SmoI CTYRAG 2 cut(s) 325, 1175
Sse9I AATT 5 cut(s) 495, 668, 795, 1024, 1104
SsiI CCGC 1 cut(s) 59
SspMI CTAG 6 cut(s) 210, 596, 683, 716, 774, 782
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 3 cut(s) 625, 832, 988
StyI CCWWGG 1 cut(s) 565
TaaI ACNGT 2 cut(s) 817, 840
TaiI ACGT 1 cut(s) 490
TasI AATT 5 cut(s) 495, 668, 795, 1024, 1104
Tru1I TTAA 5 cut(s) 390, 843, 1146, 1176, 1255
Tru9I TTAA 5 cut(s) 390, 843, 1146, 1176, 1255
TscAI CASTG 5 cut(s) 241, 319, 581, 616, 988
TseFI GTSAC 4 cut(s) 226, 310, 611, 697
TseI GCWGC 3 cut(s) 189, 192, 1010
Tsp45I GTSAC 4 cut(s) 226, 310, 611, 697
TspDTI ATGAA 2 cut(s) 282, 732
TspRI CASTG 5 cut(s) 241, 319, 581, 616, 988
Van91I CCANNNNNTGG 2 cut(s) 101, 632
Vha464I CTTAAG 1 cut(s) 1175
VpaK11BI GGWCC 1 cut(s) 562
XagI CCTNNNNNAGG 1 cut(s) 831
XapI RAATTY 2 cut(s) 668, 1104
XceI RCATGY 2 cut(s) 449, 882
XcmI CCANNNNNNNNNTGG 2 cut(s) 1052, 1212
XspI CTAG 6 cut(s) 210, 596, 683, 716, 774, 782
Zsp2I ATGCAT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.