MD17G1214900.v1.1

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0013324
Physical Location & Seq
Forward (+)
26178790 .. 26188407
9618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1214900.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGCTTCGGCTCTGCTCTCTCTGCCCTTCAACCTCCCACCCTCTTCTTCTCCTCTCTCCGCCGTACCCAATTCTCCTAAACCCAAATCCCACATCCGCCTTTTTACGAGCAGTAGGAGAAGAGCAAGAGTCACAGCCAGTGCTGCAGACCAAGAAAAAACCAAGGACACAGAGAAAGACTCAGACTCAGACTCAGCGGCTTTCAACCCCTTTGGCTTTGTCACCGATAACCCGTCAAGCCGTAGCGCCATTCAGATCAGTGAGAGCCCTGCAGAGTCTGGCAATGTTGGCCAAATGCTTTATAGGATAGAAGACAAGGGGAAGGAATATGGTAAATACTTCAGAGCGGGGGAGTATGTATGGTTTGTGAGAGAAACTGGATCAGCTGAGAGCGAACGTGGAACAGTTGTCTTTCTTCACGGTGCTCCCACGCAGTCTTACAGCTACAGAAATGTCATGTCACAGCTGTCAGAAGACTTCGGCTTCCACTGCTTAGCACCAGATTGGCTAGGATTTGGTTTCAGTGACAAACCACAGGCTGGATATGATGACTTTGATTATAAAGAAGAGGAGTTCCACAAGGAATTTGACAAATTACTGGATAAGCTCCGCGTCAAATCTCCATTCTATCTAGTCGTTCAGGGATTTCTTGTAGGTTCATATGGATTGACATGGGCATTAAAGAACCCAAGCAAAATATCAAAGCTTGCAATTCTAAACACCCCACTGACAGTTTCATCTCCCGTTCCTGGACTGTTTAAGCAATTAAGATTCCCTTTTTTAGGAGAATTTACATGCCAGAATGCAGTTATGGCCGAGCGCTTCATTGAAGCAGGTAGCGCTTACGTGTTGAAGCTCGAAAAGGCTGATGTGTATCGGTTACCATATCTTTCAAGCAGTGGACCTGGATTTGCATTACTTGAAACTACAAGGAAAGCCAATTTCAATGGTATATTAAGCCAAATATCATCTGGGTTTGCATCTGGAAGATGGGATACGCCGACTCTGGTTGTATGGGGGATTTCAGACAAGTATCTCCCTCAGTTTACAGCAGAAGAGTTTCAGAAAGCAAACCCTACCGCGGTCCAGCTTAAGTTGATAGAAGGTGCTGGGCATATGCCACAGGAAGATTGGCCCGAGAGAGTTGTTACTGCTCTACGAGTATTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

391

Amino Acids

43.4

Weight (kDa)

6.56

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 132 - 373 1.7e-11 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 135 - 377 2e-22 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 136 - 384 2.9e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 561
Acc36I ACCTGC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 539
AccBSI CCGCTC 1 cut(s) 347
AccII CGCG 2 cut(s) 612, 1082
AciI CCGC 7 cut(s) 60, 97, 197, 347, 610, 1080, 1082
AclWI GGATC 1 cut(s) 388
AcoI YGGCCR 2 cut(s) 289, 813
AcsI RAATTY 2 cut(s) 584, 788
AcuI CTGAAG 1 cut(s) 325
AfaI GTAC 1 cut(s) 66
AfeI AGCGCT 2 cut(s) 821, 841
AfiI CCNNNNNNNGG 4 cut(s) 539, 749, 782, 1081
AflII CTTAAG 1 cut(s) 1091
AflIII ACRYGT 1 cut(s) 846
AgsI TTSAA 7 cut(s) 31, 205, 830, 853, 894, 923, 946
AjnI CCWGG 2 cut(s) 748, 904
AluBI AGCT 7 cut(s) 386, 444, 466, 607, 706, 856, 1090
AluI AGCT 7 cut(s) 386, 444, 466, 607, 706, 856, 1090
Alw21I GWGCWC 1 cut(s) 427
AlwI GGATC 1 cut(s) 388
AlwNI CAGNNNCTG 1 cut(s) 278
Ama87I CYCGRG 1 cut(s) 1136
Aor51HI AGCGCT 2 cut(s) 821, 841
AoxI GGCC 3 cut(s) 289, 813, 1133
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 2 cut(s) 584, 788
Asp700I GAANNNNTTC 1 cut(s) 1059
AspLEI GCGC 3 cut(s) 248, 822, 842
AspS9I GGNCC 3 cut(s) 902, 1084, 1134
AsuHPI GGTGA 1 cut(s) 214
AvaI CYCGRG 1 cut(s) 1136
AvaII GGWCC 2 cut(s) 902, 1084
BalI TGGCCA 1 cut(s) 291
BanII GRGCYC 1 cut(s) 269
BarI GAAGNNNNNNTAC 2 cut(s) 979, 1011
BbsI GAAGAC 2 cut(s) 318, 480
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 130
BccI CCATC 1 cut(s) 984
BceAI ACGGC 2 cut(s) 47, 225
BciT130I CCWGG 2 cut(s) 750, 906
BciVI GTATCC 1 cut(s) 988
BfaI CTAG 2 cut(s) 509, 632
BfmI CTRYAG 3 cut(s) 144, 270, 445
BfoI RGCGCY 3 cut(s) 249, 823, 843
BfrI CTTAAG 1 cut(s) 1091
BfuAI ACCTGC 1 cut(s) 824
BfuI GTATCC 1 cut(s) 988
BisI GCNGC 2 cut(s) 144, 198
BlpI GCTNAGC 1 cut(s) 493
BlsI GCNGC 2 cut(s) 145, 199
Bme1390I CCNGG 2 cut(s) 750, 906
Bme18I GGWCC 2 cut(s) 902, 1084
BmeT110I CYCGRG 1 cut(s) 1136
BmgT120I GGNCC 3 cut(s) 902, 1084, 1134
BmrFI CCNGG 2 cut(s) 750, 906
BmsI GCATC 1 cut(s) 989
BpiI GAAGAC 2 cut(s) 318, 480
BplI GAGNNNNNCTC 2 cut(s) 164, 196
Bpu1102I GCTNAGC 1 cut(s) 493
BsaAI YACGTR 1 cut(s) 847
BsaBI GATNNNNATC 1 cut(s) 873
BsaJI CCNNGG 2 cut(s) 162, 1080
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 749, 782, 1081
Bse1I ACTGG 3 cut(s) 138, 382, 603
Bse3DI GCAATG 1 cut(s) 289
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 2 cut(s) 750, 906
BseDI CCNNGG 2 cut(s) 162, 1080
BseGI GGATG 1 cut(s) 93
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 4 cut(s) 539, 749, 782, 1081
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 5 cut(s) 195, 201, 207, 378, 1055
BseNI ACTGG 3 cut(s) 138, 382, 603
BseRI GAGGAG 2 cut(s) 42, 584
BseXI GCAGC 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 1109
Bsh1236I CGCG 2 cut(s) 612, 1082
BshFI GGCC 3 cut(s) 291, 815, 1135
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 1 cut(s) 1136
BslI CCNNNNNNNGG 4 cut(s) 539, 749, 782, 1081
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 3 cut(s) 291, 815, 1135
BsoBI CYCGRG 1 cut(s) 1136
Bsp1286I GDGCHC 2 cut(s) 269, 427
Bsp143I GATC 2 cut(s) 255, 380
Bsp1720I GCTNAGC 1 cut(s) 493
BspACI CCGC 7 cut(s) 60, 97, 197, 347, 610, 1080, 1082
BspANI GGCC 3 cut(s) 291, 815, 1135
BspCNI CTCAG 5 cut(s) 194, 200, 206, 379, 1054
BspFNI CGCG 2 cut(s) 612, 1082
BspMAI CTGCAG 2 cut(s) 148, 274
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 1 cut(s) 388
BspQI GCTCTTC 1 cut(s) 115
BspTI CTTAAG 1 cut(s) 1091
BsrBI CCGCTC 1 cut(s) 347
BsrDI GCAATG 1 cut(s) 289
BsrI ACTGG 3 cut(s) 138, 382, 603
BssECI CCNNGG 2 cut(s) 162, 1080
BssMI GATC 2 cut(s) 255, 380
BssT1I CCWWGG 1 cut(s) 162
Bst2UI CCWGG 2 cut(s) 750, 906
Bst4CI ACNGT 4 cut(s) 406, 422, 733, 756
Bst6I CTCTTC 4 cut(s) 49, 115, 561, 1050
BstAFI CTTAAG 1 cut(s) 1091
BstBAI YACGTR 1 cut(s) 847
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 6 cut(s) 181, 187, 193, 387, 493, 1041
BstDSI CCRYGG 1 cut(s) 1080
BstEII GGTNACC 1 cut(s) 879
BstENI CCTNNNNNAGG 1 cut(s) 780
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 612, 1082
BstH2I RGCGCY 3 cut(s) 249, 823, 843
BstHHI GCGC 3 cut(s) 248, 822, 842
BstKTI GATC 2 cut(s) 258, 383
BstMBI GATC 2 cut(s) 255, 380
BstMWI GCNNNNNNNGC 6 cut(s) 22, 143, 288, 489, 812, 839
BstNI CCWGG 2 cut(s) 750, 906
BstNSI RCATGY 1 cut(s) 798
BstPI GGTNACC 1 cut(s) 879
BstSCI CCNGG 2 cut(s) 748, 904
BstSFI CTRYAG 3 cut(s) 144, 270, 445
BstUI CGCG 2 cut(s) 612, 1082
BstV1I GCAGC 1 cut(s) 130
BstV2I GAAGAC 2 cut(s) 318, 480
BsuI GTATCC 1 cut(s) 988
BsuRI GGCC 3 cut(s) 291, 815, 1135
BtgI CCRYGG 1 cut(s) 1080
BtsCI GGATG 1 cut(s) 93
BtsI GCAGTG 2 cut(s) 487, 904
BtsIMutI CAGTG 6 cut(s) 145, 265, 487, 529, 725, 904
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 1 cut(s) 708
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 3 cut(s) 248, 822, 842
Cfr13I GGNCC 3 cut(s) 902, 1084, 1134
Cfr42I CCGCGG 1 cut(s) 1083
CseI GACGC 1 cut(s) 601
Csp6I GTAC 1 cut(s) 65
CviAII CATG 3 cut(s) 457, 672, 795
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 6 cut(s) 181, 187, 193, 387, 493, 1041
DpnI GATC 2 cut(s) 257, 382
DpnII GATC 2 cut(s) 255, 380
EaeI YGGCCR 2 cut(s) 289, 813
Eam1104I CTCTTC 4 cut(s) 49, 115, 561, 1050
EarI CTCTTC 4 cut(s) 49, 115, 561, 1050
EciI GGCGGA 2 cut(s) 49, 86
Eco130I CCWWGG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 269
Eco47I GGWCC 2 cut(s) 902, 1084
Eco47III AGCGCT 2 cut(s) 821, 841
Eco57I CTGAAG 1 cut(s) 325
Eco88I CYCGRG 1 cut(s) 1136
Eco91I GGTNACC 1 cut(s) 879
EcoNI CCTNNNNNAGG 1 cut(s) 780
EcoO65I GGTNACC 1 cut(s) 879
EcoRII CCWGG 2 cut(s) 748, 904
EcoT14I CCWWGG 1 cut(s) 162
EcoT38I GRGCYC 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 162
FaeI CATG 3 cut(s) 460, 675, 798
FalI AAGNNNNNCTT 2 cut(s) 760, 792
FatI CATG 3 cut(s) 456, 671, 794
FauI CCCGC 1 cut(s) 340
FauNDI CATATG 2 cut(s) 661, 1116
Fnu4HI GCNGC 2 cut(s) 144, 198
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 1 cut(s) 269
Fsp4HI GCNGC 2 cut(s) 144, 198
FspBI CTAG 2 cut(s) 509, 632
GlaI GCGC 3 cut(s) 247, 821, 841
GluI GCNGC 2 cut(s) 144, 198
GsaI CCCAGC 1 cut(s) 1113
HaeII RGCGCY 3 cut(s) 249, 823, 843
HaeIII GGCC 3 cut(s) 291, 815, 1135
HgaI GACGC 1 cut(s) 601
HhaI GCGC 3 cut(s) 248, 822, 842
Hin1II CATG 3 cut(s) 460, 675, 798
Hin6I GCGC 3 cut(s) 246, 820, 840
HinP1I GCGC 3 cut(s) 246, 820, 840
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 7 cut(s) 129, 179, 185, 191, 275, 771, 1003
HphI GGTGA 1 cut(s) 214
Hpy166II GTNNAC 2 cut(s) 902, 1047
Hpy188I TCNGA 7 cut(s) 184, 190, 255, 344, 472, 1027, 1065
Hpy188III TCNNGA 1 cut(s) 984
Hpy8I GTNNAC 2 cut(s) 902, 1047
HpyAV CCTTC 3 cut(s) 37, 316, 1097
HpyCH4III ACNGT 4 cut(s) 406, 422, 733, 756
HpyCH4IV ACGT 2 cut(s) 397, 846
HpyCH4V TGCA 6 cut(s) 146, 272, 710, 806, 914, 980
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 143, 288, 489, 812, 839
HpyF3I CTNAG 6 cut(s) 181, 187, 193, 387, 493, 1041
HpySE526I ACGT 2 cut(s) 397, 846
Hsp92II CATG 3 cut(s) 460, 675, 798
HspAI GCGC 3 cut(s) 246, 820, 840
KspI CCGCGG 1 cut(s) 1083
Kzo9I GATC 2 cut(s) 255, 380
LguI GCTCTTC 1 cut(s) 115
LmnI GCTCC 2 cut(s) 430, 612
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 1 cut(s) 989
MaeI CTAG 2 cut(s) 509, 632
MaeII ACGT 2 cut(s) 397, 846
MaeIII GTNAC 6 cut(s) 130, 220, 459, 524, 879, 1147
MalI GATC 2 cut(s) 257, 382
MbiI CCGCTC 1 cut(s) 347
MboI GATC 2 cut(s) 255, 380
MhlI GDGCHC 2 cut(s) 269, 427
MlsI TGGCCA 1 cut(s) 291
MluCI AATT 7 cut(s) 70, 584, 593, 711, 764, 788, 940
MluNI TGGCCA 1 cut(s) 291
MlyI GAGTC 6 cut(s) 138, 173, 179, 185, 284, 997
MnlI CCTC 5 cut(s) 44, 52, 63, 562, 1050
Mox20I TGGCCA 1 cut(s) 291
MroXI GAANNNNTTC 1 cut(s) 1059
MscI TGGCCA 1 cut(s) 291
MseI TTAA 5 cut(s) 680, 759, 767, 956, 1092
Msp20I TGGCCA 1 cut(s) 291
MspA1I CMGCKG 4 cut(s) 197, 386, 466, 1082
MspCI CTTAAG 1 cut(s) 1091
MspR9I CCNGG 2 cut(s) 750, 906
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 2 cut(s) 750, 906
MvnI CGCG 2 cut(s) 612, 1082
MwoI GCNNNNNNNGC 6 cut(s) 22, 143, 288, 489, 812, 839
NdeI CATATG 2 cut(s) 661, 1116
NdeII GATC 2 cut(s) 255, 380
NlaIII CATG 3 cut(s) 460, 675, 798
NmeAIII GCCGAG 1 cut(s) 841
NmuCI GTSAC 4 cut(s) 130, 220, 459, 524
NspI RCATGY 1 cut(s) 798
PciSI GCTCTTC 1 cut(s) 115
PctI GAATGC 1 cut(s) 808
PdmI GAANNNNTTC 1 cut(s) 1059
PfeI GAWTC 1 cut(s) 771
PflMI CCANNNNNTGG 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 748
PkrI GCNGC 2 cut(s) 145, 199
PleI GAGTC 6 cut(s) 137, 173, 179, 185, 283, 997
PpsI GAGTC 6 cut(s) 137, 173, 179, 185, 283, 997
Ppu21I YACGTR 1 cut(s) 847
PsiI TTATAA 1 cut(s) 561
Psp6I CCWGG 2 cut(s) 748, 904
PspEI GGTNACC 1 cut(s) 879
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 2 cut(s) 748, 904
PspPI GGNCC 3 cut(s) 902, 1084, 1134
PstI CTGCAG 2 cut(s) 148, 274
PstNI CAGNNNCTG 1 cut(s) 278
PvuII CAGCTG 2 cut(s) 386, 466
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SacII CCGCGG 1 cut(s) 1083
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 5 cut(s) 680, 759, 767, 956, 1092
SatI GCNGC 2 cut(s) 144, 198
Sau3AI GATC 2 cut(s) 255, 380
Sau96I GGNCC 3 cut(s) 902, 1084, 1134
SchI GAGTC 6 cut(s) 138, 173, 179, 185, 284, 997
ScrFI CCNGG 2 cut(s) 750, 906
SduI GDGCHC 2 cut(s) 269, 427
SfaNI GCATC 1 cut(s) 989
SfcI CTRYAG 3 cut(s) 144, 270, 445
Sfr303I CCGCGG 1 cut(s) 1083
SgrBI CCGCGG 1 cut(s) 1083
SinI GGWCC 2 cut(s) 902, 1084
SmlI CTYRAG 1 cut(s) 1091
SmoI CTYRAG 1 cut(s) 1091
Sse9I AATT 7 cut(s) 70, 584, 593, 711, 764, 788, 940
SsiI CCGC 7 cut(s) 60, 97, 197, 347, 610, 1080, 1082
SspMI CTAG 2 cut(s) 509, 632
StyD4I CCNGG 2 cut(s) 748, 904
StyI CCWWGG 1 cut(s) 162
TaaI ACNGT 4 cut(s) 406, 422, 733, 756
TaiI ACGT 2 cut(s) 400, 849
TaqI TCGA 1 cut(s) 858
TasI AATT 7 cut(s) 70, 584, 593, 711, 764, 788, 940
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 771
Tru1I TTAA 5 cut(s) 680, 759, 767, 956, 1092
Tru9I TTAA 5 cut(s) 680, 759, 767, 956, 1092
TscAI CASTG 6 cut(s) 145, 265, 494, 529, 732, 904
TseFI GTSAC 4 cut(s) 130, 220, 459, 524
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 4 cut(s) 130, 220, 459, 524
TspDTI ATGAA 3 cut(s) 648, 726, 814
TspRI CASTG 6 cut(s) 145, 265, 494, 529, 732, 904
Van91I CCANNNNNTGG 1 cut(s) 539
Vha464I CTTAAG 1 cut(s) 1091
VpaK11BI GGWCC 2 cut(s) 902, 1084
XagI CCTNNNNNAGG 1 cut(s) 780
XapI RAATTY 2 cut(s) 584, 788
XceI RCATGY 1 cut(s) 798
XcmI CCANNNNNNNNNTGG 2 cut(s) 968, 1128
XmnI GAANNNNTTC 1 cut(s) 1059
XspI CTAG 2 cut(s) 509, 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.