RLG00000019426

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0013324
Physical Location & Seq
Reverse (-)
51928628 .. 51943548
14921 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019426

Sequence Viewer

Length: 1083 bp
ATGGCTTGGGCTTCTCCTCCTCTGCTCTCTCTCCCTCTCAACCTCCTCCCACCCTCCTCTTCTCCTCTCAACTCCAACTCCCACACACGCAGCAGCTGGCTCATAACCACTAGAACATCATCAAGAGTCACAGCCAGTGCTTCAGACCAAGACCAAACCAACAACAAAGAAAAACAACAAGACTCACCAGCTTTCAATCCCTTTGGTTTTGTCACTGATAACCCGTCAAGCCGTAGCGCTATTCAGCTCCCTGATAACCCTGCAGACGATGGCAATGTTGGCCAAATGCTTAATAGAATCGAAGACAAGGGGAGGGATTATGGAAAATACATCAGGTCAGGGGAGCATGTATGGTTCGTGAGAGAAACTGGATCAGCTGAGAGCCAACGTGGAACAATTGTCTTCCTTCACGGGGCTCCCACTCAGTCTTATAGCTACAGAAATGTTATGTCTCAGATGTCGGACCTTGGGTTCCACTGCTTTGCACCTGATTGGCTAGGATTTGGTTTCAGTGACAAACCACAGCCAGGATATGGATTTGACTACACAGAAAAGGAGTTCCACGACGAATTTGACAGATTACTAGATGTGCTAGGGGTCACATCTCCTTTCTATCTAGTCGTTCAGGGATTTCTTGTAGGTTCATATGGATTGACATGGGCAGTAAAAAATCCTAGCAGACTAGAGAAGCTTGCAATTCTAAATAGTCCACTCACCGTGTCATCTCCTCTTCCAGGACTGTTTAAGCAACTAAGTTATGCATTGAAGCTGGAAAAGGCTGATGTGTATCGGTTACCTTATCTGTCAAGCAGTGGCCCTGGATTTGCTATACTTGAAGCTGCAAGGAAAGCTAATTTCCAAAGCATCCTGAGCCAAATAGCATCTGGCTTTGCATCTGGAAGATGGGATATACCGACTCTTGTTGCATGGGGAATTTCAGACAAGTATCTACCTCAATCTGTGGCGGAAGAGTTTAAGAAAGCAAACCCCAATGTTGTCAACCTTAAGTTGATAGAAGGTGCCGGGCATATGCCACAGGAGGACTGGCCTGAAAGAGTTGTTACTGCTCTGCGAAATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

361

Amino Acids

39.83

Weight (kDa)

6.1

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 128 - 243 4.9e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 132 - 276 6.1e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 133 - 355 6.9e-12 Alpha/beta hydrolase family
Abhydrolase_1 PF00561 281 - 348 1.8e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1019
AccB7I CCANNNNNTGG 1 cut(s) 533
AciI CCGC 1 cut(s) 965
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 2 cut(s) 569, 933
AcuI CTGAAG 1 cut(s) 126
AfeI AGCGCT 1 cut(s) 238
AfiI CCNNNNNNNGG 4 cut(s) 412, 527, 533, 734
AflII CTTAAG 1 cut(s) 1004
AgsI TTSAA 3 cut(s) 196, 766, 836
AjnI CCWGG 3 cut(s) 526, 733, 817
AloI GAACNNNNNNTCC 2 cut(s) 455, 487
AluBI AGCT 9 cut(s) 96, 191, 247, 377, 435, 691, 769, 839, 851
AluI AGCT 9 cut(s) 96, 191, 247, 377, 435, 691, 769, 839, 851
Alw26I GTCTC 1 cut(s) 456
AlwI GGATC 1 cut(s) 379
AlwNI CAGNNNCTG 1 cut(s) 96
Aor51HI AGCGCT 1 cut(s) 238
AoxI GGCC 3 cut(s) 280, 814, 1046
ApeKI GCWGC 3 cut(s) 90, 93, 839
ApoI RAATTY 2 cut(s) 569, 933
AspLEI GCGC 1 cut(s) 239
AspS9I GGNCC 2 cut(s) 463, 815
AsuC2I CCSGG 1 cut(s) 1024
AsuHPI GGTGA 2 cut(s) 177, 706
AvaII GGWCC 1 cut(s) 463
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 1 cut(s) 1019
BanII GRGCYC 1 cut(s) 418
BbsI GAAGAC 2 cut(s) 309, 394
BbvI GCAGC 3 cut(s) 102, 105, 826
BccI CCATC 2 cut(s) 263, 897
BceAI ACGGC 1 cut(s) 216
BciT130I CCWGG 3 cut(s) 528, 735, 819
BcnI CCSGG 1 cut(s) 1024
BcoDI GTCTC 1 cut(s) 456
BfaI CTAG 7 cut(s) 111, 497, 584, 593, 617, 675, 683
BfmI CTRYAG 2 cut(s) 261, 436
BfoI RGCGCY 1 cut(s) 240
BfrI CTTAAG 1 cut(s) 1004
BisI GCNGC 3 cut(s) 91, 94, 840
BlsI GCNGC 3 cut(s) 92, 95, 841
Bme1390I CCNGG 4 cut(s) 528, 735, 819, 1024
Bme18I GGWCC 1 cut(s) 463
BmgT120I GGNCC 2 cut(s) 463, 815
BmiI GGNNCC 3 cut(s) 417, 473, 1021
BmrFI CCNGG 4 cut(s) 528, 735, 819, 1024
BmsI GCATC 3 cut(s) 873, 890, 902
BpiI GAAGAC 2 cut(s) 309, 394
Bpu10I CCTNAGC 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 1024
BsaBI GATNNNNATC 1 cut(s) 786
BsaJI CCNNGG 2 cut(s) 466, 817
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 4 cut(s) 412, 527, 533, 734
Bse1I ACTGG 3 cut(s) 135, 373, 1049
Bse3DI GCAATG 1 cut(s) 280
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 3 cut(s) 528, 735, 819
BseDI CCNNGG 2 cut(s) 466, 817
BseGI GGATG 1 cut(s) 864
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 4 cut(s) 412, 527, 533, 734
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 4 cut(s) 369, 437, 467, 860
BseNI ACTGG 3 cut(s) 135, 373, 1049
BseRI GAGGAG 6 cut(s) 6, 9, 35, 46, 54, 717
BseXI GCAGC 3 cut(s) 102, 105, 826
BshFI GGCC 3 cut(s) 282, 816, 1048
BshNI GGYRCC 1 cut(s) 1019
BsiSI CCGG 1 cut(s) 1023
BslI CCNNNNNNNGG 4 cut(s) 412, 527, 533, 734
BsmAI GTCTC 1 cut(s) 456
BsnI GGCC 3 cut(s) 282, 816, 1048
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 1 cut(s) 371
BspACI CCGC 1 cut(s) 965
BspANI GGCC 3 cut(s) 282, 816, 1048
BspCNI CTCAG 4 cut(s) 370, 436, 466, 861
BspLI GGNNCC 3 cut(s) 417, 473, 1021
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 1019
BspTI CTTAAG 1 cut(s) 1004
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 3 cut(s) 135, 373, 1049
BssECI CCNNGG 2 cut(s) 466, 817
BssMI GATC 1 cut(s) 371
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 3 cut(s) 528, 735, 819
Bst4CI ACNGT 2 cut(s) 718, 741
Bst6I CTCTTC 3 cut(s) 64, 735, 963
BstAFI CTTAAG 1 cut(s) 1004
BstC8I GCNNGC 2 cut(s) 98, 693
BstDEI CTNAG 5 cut(s) 378, 423, 453, 752, 869
BstEII GGTNACC 1 cut(s) 792
BstENI CCTNNNNNAGG 1 cut(s) 732
BstF5I GGATG 1 cut(s) 864
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 1 cut(s) 374
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 1 cut(s) 371
BstMWI GCNNNNNNNGC 3 cut(s) 279, 848, 870
BstNI CCWGG 3 cut(s) 528, 735, 819
BstNSI RCATGY 1 cut(s) 350
BstPI GGTNACC 1 cut(s) 792
BstSCI CCNGG 4 cut(s) 526, 733, 817, 1022
BstSFI CTRYAG 2 cut(s) 261, 436
BstV1I GCAGC 3 cut(s) 102, 105, 826
BstV2I GAAGAC 2 cut(s) 309, 394
BsuRI GGCC 3 cut(s) 282, 816, 1048
BtsCI GGATG 1 cut(s) 864
BtsI GCAGTG 2 cut(s) 475, 817
BtsIMutI CAGTG 5 cut(s) 142, 213, 475, 517, 817
Cac8I GCNNGC 2 cut(s) 98, 693
CaiI CAGNNNCTG 1 cut(s) 96
CfoI GCGC 1 cut(s) 239
Cfr13I GGNCC 2 cut(s) 463, 815
CviAII CATG 3 cut(s) 347, 657, 927
DdeI CTNAG 5 cut(s) 378, 423, 453, 752, 869
DpnI GATC 1 cut(s) 373
DpnII GATC 1 cut(s) 371
EaeI YGGCCR 1 cut(s) 280
Eam1104I CTCTTC 3 cut(s) 64, 735, 963
EarI CTCTTC 3 cut(s) 64, 735, 963
EciI GGCGGA 1 cut(s) 980
Eco130I CCWWGG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 418
Eco47I GGWCC 1 cut(s) 463
Eco47III AGCGCT 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 126
Eco91I GGTNACC 1 cut(s) 792
EcoNI CCTNNNNNAGG 1 cut(s) 732
EcoO65I GGTNACC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 526, 733, 817
EcoT14I CCWWGG 1 cut(s) 466
EcoT22I ATGCAT 1 cut(s) 763
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 3 cut(s) 350, 660, 930
FatI CATG 3 cut(s) 346, 656, 926
FauNDI CATATG 2 cut(s) 646, 1029
Fnu4HI GCNGC 3 cut(s) 91, 94, 840
FokI GGATG 1 cut(s) 851
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 3 cut(s) 91, 94, 840
FspBI CTAG 7 cut(s) 111, 497, 584, 593, 617, 675, 683
GlaI GCGC 1 cut(s) 238
GluI GCNGC 3 cut(s) 91, 94, 840
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 3 cut(s) 282, 816, 1048
HapII CCGG 1 cut(s) 1023
HhaI GCGC 1 cut(s) 239
Hin1II CATG 3 cut(s) 350, 660, 930
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HincII GTYRAC 1 cut(s) 1000
HindII GTYRAC 1 cut(s) 1000
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 126, 182, 297, 916
HpaII CCGG 1 cut(s) 1023
HphI GGTGA 2 cut(s) 177, 706
Hpy166II GTNNAC 2 cut(s) 710, 1000
Hpy188I TCNGA 4 cut(s) 145, 456, 463, 940
Hpy188III TCNNGA 4 cut(s) 123, 358, 868, 897
Hpy8I GTNNAC 2 cut(s) 710, 1000
Hpy99I CGWCG 1 cut(s) 569
HpyAV CCTTC 2 cut(s) 416, 1010
HpyCH4III ACNGT 2 cut(s) 718, 741
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 7 cut(s) 263, 485, 695, 761, 842, 893, 926
HpyF10VI GCNNNNNNNGC 3 cut(s) 279, 848, 870
HpyF3I CTNAG 5 cut(s) 378, 423, 453, 752, 869
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 3 cut(s) 350, 660, 930
HspAI GCGC 1 cut(s) 237
Kzo9I GATC 1 cut(s) 371
LmnI GCTCC 3 cut(s) 252, 343, 421
Lsp1109I GCAGC 3 cut(s) 102, 105, 826
LweI GCATC 3 cut(s) 873, 890, 902
MaeI CTAG 7 cut(s) 111, 497, 584, 593, 617, 675, 683
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 6 cut(s) 127, 211, 512, 598, 792, 1060
MalI GATC 1 cut(s) 373
MboI GATC 1 cut(s) 371
MboII GAAGA 6 cut(s) 51, 314, 394, 722, 912, 980
MfeI CAATTG 1 cut(s) 396
MhlI GDGCHC 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 5 cut(s) 396, 569, 696, 853, 933
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 3 cut(s) 135, 176, 910
MmeI TCCRAC 2 cut(s) 99, 441
Mox20I TGGCCA 1 cut(s) 282
Mph1103I ATGCAT 1 cut(s) 763
MscI TGGCCA 1 cut(s) 282
MseI TTAA 4 cut(s) 291, 744, 975, 1005
Msp20I TGGCCA 1 cut(s) 282
MspA1I CMGCKG 2 cut(s) 96, 377
MspCI CTTAAG 1 cut(s) 1004
MspI CCGG 1 cut(s) 1023
MspR9I CCNGG 4 cut(s) 528, 735, 819, 1024
MunI CAATTG 1 cut(s) 396
MvaI CCWGG 3 cut(s) 528, 735, 819
MwoI GCNNNNNNNGC 3 cut(s) 279, 848, 870
NciI CCSGG 1 cut(s) 1024
NdeI CATATG 2 cut(s) 646, 1029
NdeII GATC 1 cut(s) 371
NlaIII CATG 3 cut(s) 350, 660, 930
NlaIV GGNNCC 3 cut(s) 417, 473, 1021
NmuCI GTSAC 4 cut(s) 127, 211, 512, 598
NsiI ATGCAT 1 cut(s) 763
NspI RCATGY 1 cut(s) 350
PfeI GAWTC 1 cut(s) 297
PflMI CCANNNNNTGG 1 cut(s) 533
PfoI TCCNGGA 1 cut(s) 733
PkrI GCNGC 3 cut(s) 92, 95, 841
PleI GAGTC 3 cut(s) 134, 176, 910
PpsI GAGTC 3 cut(s) 134, 176, 910
Psp6I CCWGG 3 cut(s) 526, 733, 817
PspEI GGTNACC 1 cut(s) 792
PspGI CCWGG 3 cut(s) 526, 733, 817
PspN4I GGNNCC 3 cut(s) 417, 473, 1021
PspPI GGNCC 2 cut(s) 463, 815
PstI CTGCAG 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 96
PvuII CAGCTG 2 cut(s) 96, 377
SaqAI TTAA 4 cut(s) 291, 744, 975, 1005
SatI GCNGC 3 cut(s) 91, 94, 840
Sau3AI GATC 1 cut(s) 371
Sau96I GGNCC 2 cut(s) 463, 815
SchI GAGTC 3 cut(s) 135, 176, 910
ScrFI CCNGG 4 cut(s) 528, 735, 819, 1024
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 3 cut(s) 873, 890, 902
SfcI CTRYAG 2 cut(s) 261, 436
SinI GGWCC 1 cut(s) 463
SmlI CTYRAG 1 cut(s) 1004
SmoI CTYRAG 1 cut(s) 1004
Sse9I AATT 5 cut(s) 396, 569, 696, 853, 933
SsiI CCGC 1 cut(s) 965
SspMI CTAG 7 cut(s) 111, 497, 584, 593, 617, 675, 683
StyD4I CCNGG 4 cut(s) 526, 733, 817, 1022
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 2 cut(s) 718, 741
TaiI ACGT 1 cut(s) 391
TaqI TCGA 1 cut(s) 300
TasI AATT 5 cut(s) 396, 569, 696, 853, 933
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 4 cut(s) 291, 744, 975, 1005
Tru9I TTAA 4 cut(s) 291, 744, 975, 1005
TscAI CASTG 5 cut(s) 142, 220, 482, 517, 817
TseFI GTSAC 4 cut(s) 127, 211, 512, 598
TseI GCWGC 3 cut(s) 90, 93, 839
Tsp45I GTSAC 4 cut(s) 127, 211, 512, 598
TspDTI ATGAA 1 cut(s) 633
TspRI CASTG 5 cut(s) 142, 220, 482, 517, 817
Van91I CCANNNNNTGG 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 1004
VpaK11BI GGWCC 1 cut(s) 463
XagI CCTNNNNNAGG 1 cut(s) 732
XapI RAATTY 2 cut(s) 569, 933
XceI RCATGY 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 2 cut(s) 881, 1041
XspI CTAG 7 cut(s) 111, 497, 584, 593, 617, 675, 683
Zsp2I ATGCAT 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.