pycom17g21940

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013324
Physical Location & Seq
Forward (+)
20352951 .. 20356883
3933 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g21940.11

Sequence Viewer

Length: 1215 bp
ATGAAGATGAGTATACTCCAACAGCCACAACAACTAGTGAGAATGGCTTCGGCTCTGCTCTCTCTGCCCTTCAACCTCCCAGCCTCTTCTTCTCCTCTCTCCGCCGTACCCAATTCTCCTAAACCCAAATCCCACATCCCCCTTTTTACAAGCAGTAGGAGAAGAGCAAGAGTCACAGCCAGTGCTGCAGACCAAGAAAAAACCAAGGACACAGAGAAAGACTCAGACTCAGACTCACCGGCTTTCAACCCCTTTGGCTTTGTCACCGATAACCCATCAAGCCGTAGTGCCATTCAGATCAGTGAGAGCCCTGCAGAGTCTGGCAATGTTGGCCAAATGCTTTATAGGATAGAAGACAAGGGGAAGGAATATGGTAAATACTTCAGAGCAGGGGAGTATGTATGGTTTGTGAGAGAAACTGGATCAGCTGAGAGCGAACGTGGAACAGTTGTCTTTCTTCACGGTGCTCCCACGCAGTCTTACAGCTACAGAAGTGTCATGTCACAGCTGTCAGAAGACTTCGGCTTCCACTGCTTAGCACCAGATTGGCTAGGATTTGGTTTCAGTGACAAGCCACAGGCTGGATATGATGACTTTGATTACAAAGAAGAGGAGTTCCACAAGGAATTTGACAAATTGCTGGATAAGCTCCGCGTCAAATCTCCTTTCTATCTAGTCGTTCAGGGATTTCTTGTAGGTTCATATGGACTGACATGGGCATTAAAGAACCCAAGCAGAATATCAAAGCTTGCAATTCTAAACACTCCACTGACAGTTTCATCTCTCGTTCCTGGACTGTTTAAGCAATTAAGATTCCCTTTTTTAGGTGAATTTACATGCCAGAATGCAGTTATGGCTGAGCGCTTCATTGAAGCAGGTAGCGCTTACGTGTTGAAGCTCGAAAAGGCTGATGTGTATCGGTTACCATATCTTTCAAGCAGTGGACCTGGATTTGCGTTACTTGAAACTACAAGGAAAGCCGATTTCAATGGTATATTAAGCCAAATATCATCTGGGTTTGCATCTGGAAGATGGGATATGCCGACTCTGGTTGTATGGGGGATTTCAGACAAGTATCTCCCTCAGTCTATAGCAGAAGAGTTTCAGAAAGCAAACCCTACTGCGGTCCAGCTTAAGTTGATAGAAGGTGCCGGGCATATGCCACAGGAGGATTGGCCCGAGAGAGTTGTTACTGCTCTACGAGTATTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6 Hydrolase_4

Protein Analysis

405

Amino Acids

45.02

Weight (kDa)

6.56

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013324)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52510 AT1G52510
fragaria_vesca FvH4_6g29410 FvH4_6g29410
malus_domestica MD17G1214900.v1.1
prunus_persica Prupe.3G090600_v2.0.a1
pyrus_communis pycom17g21940
rosa_chinensis RchiOBHm_Chr2g0134871
rosa_laevigata RLG00000019426
rosa_multiflora Rmu_sc0004406.1_g000013 Rmu_sc0004564.1_g000002
rosa_roxburghii Rroxscaffold_2G00109700
rosa_rugosa Rorug02G0320600
rosa_samantha Rh2AG373200 Rh2BG378200 Rh2CG356700 Rh2DG395000
rosa_wichuraiana Rw2G030330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 1148
AccB7I CCANNNNNTGG 1 cut(s) 581
AccI GTMKAC 1 cut(s) 13
AccII CGCG 1 cut(s) 654
AciI CCGC 3 cut(s) 102, 652, 1124
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 2 cut(s) 626, 830
AcuI CTGAAG 1 cut(s) 367
AfaI GTAC 1 cut(s) 108
AfeI AGCGCT 2 cut(s) 863, 883
AfiI CCNNNNNNNGG 3 cut(s) 581, 824, 1123
AflII CTTAAG 1 cut(s) 1133
AflIII ACRYGT 1 cut(s) 888
AgsI TTSAA 7 cut(s) 73, 247, 872, 895, 936, 965, 988
AhlI ACTAGT 1 cut(s) 34
AjnI CCWGG 2 cut(s) 790, 946
AluBI AGCT 7 cut(s) 428, 486, 508, 649, 748, 898, 1132
AluI AGCT 7 cut(s) 428, 486, 508, 649, 748, 898, 1132
Alw21I GWGCWC 1 cut(s) 469
AlwI GGATC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 1178
Aor51HI AGCGCT 2 cut(s) 863, 883
AoxI GGCC 2 cut(s) 331, 1175
ApeKI GCWGC 1 cut(s) 185
ApoI RAATTY 2 cut(s) 626, 830
Asp700I GAANNNNTTC 2 cut(s) 46, 1101
AspLEI GCGC 2 cut(s) 864, 884
AspS9I GGNCC 3 cut(s) 944, 1126, 1176
AsuC2I CCSGG 1 cut(s) 1153
AsuHPI GGTGA 3 cut(s) 228, 256, 839
AvaI CYCGRG 1 cut(s) 1178
AvaII GGWCC 2 cut(s) 944, 1126
BalI TGGCCA 1 cut(s) 333
BanI GGYRCC 1 cut(s) 1148
BanII GRGCYC 1 cut(s) 311
BbsI GAAGAC 2 cut(s) 360, 522
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 1 cut(s) 172
BccI CCATC 2 cut(s) 283, 1026
BceAI ACGGC 2 cut(s) 89, 267
BciT130I CCWGG 2 cut(s) 792, 948
BcnI CCSGG 1 cut(s) 1153
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 3 cut(s) 35, 551, 674
BfmI CTRYAG 4 cut(s) 186, 312, 487, 1089
BfoI RGCGCY 2 cut(s) 865, 885
BfrI CTTAAG 1 cut(s) 1133
BfuAI ACCTGC 1 cut(s) 866
BisI GCNGC 1 cut(s) 186
BlpI GCTNAGC 2 cut(s) 535, 858
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 3 cut(s) 792, 948, 1153
Bme18I GGWCC 2 cut(s) 944, 1126
BmeT110I CYCGRG 1 cut(s) 1178
BmgT120I GGNCC 3 cut(s) 944, 1126, 1176
BmiI GGNNCC 1 cut(s) 1150
BmrFI CCNGG 3 cut(s) 792, 948, 1153
BmsI GCATC 1 cut(s) 1031
BpiI GAAGAC 2 cut(s) 360, 522
BplI GAGNNNNNCTC 2 cut(s) 206, 238
Bpu1102I GCTNAGC 2 cut(s) 535, 858
BpuMI CCSGG 1 cut(s) 1153
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 204
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 824, 1123
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 2 cut(s) 180, 424
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 915
BseBI CCWGG 2 cut(s) 792, 948
BseDI CCNNGG 1 cut(s) 204
BseGI GGATG 1 cut(s) 135
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 3 cut(s) 581, 824, 1123
BseMI GCAATG 1 cut(s) 331
BseMII CTCAG 5 cut(s) 237, 243, 420, 849, 1097
BseNI ACTGG 2 cut(s) 180, 424
BseRI GAGGAG 2 cut(s) 84, 626
BseXI GCAGC 1 cut(s) 172
BseYI CCCAGC 1 cut(s) 79
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 2 cut(s) 333, 1177
BshNI GGYRCC 1 cut(s) 1148
BsiHKAI GWGCWC 1 cut(s) 469
BsiHKCI CYCGRG 1 cut(s) 1178
BsiSI CCGG 2 cut(s) 239, 1152
BslI CCNNNNNNNGG 3 cut(s) 581, 824, 1123
BsmI GAATGC 1 cut(s) 850
BsnI GGCC 2 cut(s) 333, 1177
BsoBI CYCGRG 1 cut(s) 1178
Bsp1286I GDGCHC 2 cut(s) 311, 469
Bsp143I GATC 2 cut(s) 297, 422
Bsp1720I GCTNAGC 2 cut(s) 535, 858
BspACI CCGC 3 cut(s) 102, 652, 1124
BspANI GGCC 2 cut(s) 333, 1177
BspCNI CTCAG 5 cut(s) 236, 242, 421, 850, 1096
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 1 cut(s) 1150
BspMAI CTGCAG 2 cut(s) 190, 316
BspMI ACCTGC 1 cut(s) 866
BspPI GGATC 1 cut(s) 430
BspQI GCTCTTC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 1148
BspTI CTTAAG 1 cut(s) 1133
BsrDI GCAATG 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 238
BsrI ACTGG 2 cut(s) 180, 424
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 204
BssMI GATC 2 cut(s) 297, 422
BssNAI GTATAC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 204
Bst1107I GTATAC 1 cut(s) 14
Bst2UI CCWGG 2 cut(s) 792, 948
Bst4CI ACNGT 4 cut(s) 448, 464, 775, 798
Bst6I CTCTTC 4 cut(s) 91, 157, 603, 1092
BstAFI CTTAAG 1 cut(s) 1133
BstBAI YACGTR 1 cut(s) 889
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 6 cut(s) 223, 229, 429, 535, 858, 1083
BstEII GGTNACC 1 cut(s) 921
BstENI CCTNNNNNAGG 1 cut(s) 822
BstF5I GGATG 1 cut(s) 135
BstFNI CGCG 1 cut(s) 654
BstH2I RGCGCY 2 cut(s) 865, 885
BstHHI GCGC 2 cut(s) 864, 884
BstKTI GATC 2 cut(s) 300, 425
BstMBI GATC 2 cut(s) 297, 422
BstMWI GCNNNNNNNGC 7 cut(s) 64, 185, 330, 531, 646, 854, 881
BstNI CCWGG 2 cut(s) 792, 948
BstNSI RCATGY 1 cut(s) 840
BstPI GGTNACC 1 cut(s) 921
BstSCI CCNGG 3 cut(s) 790, 946, 1151
BstSFI CTRYAG 4 cut(s) 186, 312, 487, 1089
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 172
BstV2I GAAGAC 2 cut(s) 360, 522
BstZ17I GTATAC 1 cut(s) 14
BsuRI GGCC 2 cut(s) 333, 1177
BtsCI GGATG 1 cut(s) 135
BtsI GCAGTG 2 cut(s) 529, 946
BtsIMutI CAGTG 6 cut(s) 187, 307, 529, 571, 767, 946
BveI ACCTGC 1 cut(s) 866
Cac8I GCNNGC 1 cut(s) 750
CaiI CAGNNNCTG 1 cut(s) 320
CfoI GCGC 2 cut(s) 864, 884
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 3 cut(s) 944, 1126, 1176
CseI GACGC 1 cut(s) 643
Csp6I GTAC 1 cut(s) 107
CviAII CATG 3 cut(s) 499, 714, 837
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 6 cut(s) 223, 229, 429, 535, 858, 1083
DpnI GATC 2 cut(s) 299, 424
DpnII GATC 2 cut(s) 297, 422
EaeI YGGCCR 1 cut(s) 331
Eam1104I CTCTTC 4 cut(s) 91, 157, 603, 1092
EarI CTCTTC 4 cut(s) 91, 157, 603, 1092
EciI GGCGGA 1 cut(s) 91
Eco130I CCWWGG 1 cut(s) 204
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 2 cut(s) 944, 1126
Eco47III AGCGCT 2 cut(s) 863, 883
Eco57I CTGAAG 1 cut(s) 367
Eco88I CYCGRG 1 cut(s) 1178
Eco91I GGTNACC 1 cut(s) 921
EcoNI CCTNNNNNAGG 1 cut(s) 822
EcoO65I GGTNACC 1 cut(s) 921
EcoRII CCWGG 2 cut(s) 790, 946
EcoT14I CCWWGG 1 cut(s) 204
EcoT38I GRGCYC 1 cut(s) 311
ErhI CCWWGG 1 cut(s) 204
FaeI CATG 3 cut(s) 502, 717, 840
FalI AAGNNNNNCTT 2 cut(s) 802, 834
FatI CATG 3 cut(s) 498, 713, 836
FauNDI CATATG 2 cut(s) 703, 1158
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 186
FspBI CTAG 3 cut(s) 35, 551, 674
GlaI GCGC 2 cut(s) 863, 883
GluI GCNGC 1 cut(s) 186
GsaI CCCAGC 1 cut(s) 83
HaeII RGCGCY 2 cut(s) 865, 885
HaeIII GGCC 2 cut(s) 333, 1177
HapII CCGG 2 cut(s) 239, 1152
HgaI GACGC 1 cut(s) 643
HhaI GCGC 2 cut(s) 864, 884
Hin1II CATG 3 cut(s) 502, 717, 840
Hin6I GCGC 2 cut(s) 862, 882
HinP1I GCGC 2 cut(s) 862, 882
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 7 cut(s) 171, 221, 227, 233, 317, 813, 1045
HpaII CCGG 2 cut(s) 239, 1152
HphI GGTGA 3 cut(s) 228, 256, 839
Hpy166II GTNNAC 2 cut(s) 14, 944
Hpy188I TCNGA 7 cut(s) 226, 232, 297, 386, 514, 1069, 1107
Hpy188III TCNNGA 1 cut(s) 1026
Hpy8I GTNNAC 2 cut(s) 14, 944
HpyAV CCTTC 3 cut(s) 79, 358, 1139
HpyCH4III ACNGT 4 cut(s) 448, 464, 775, 798
HpyCH4IV ACGT 2 cut(s) 439, 888
HpyCH4V TGCA 5 cut(s) 188, 314, 752, 848, 1022
HpyF10VI GCNNNNNNNGC 7 cut(s) 64, 185, 330, 531, 646, 854, 881
HpyF3I CTNAG 6 cut(s) 223, 229, 429, 535, 858, 1083
HpySE526I ACGT 2 cut(s) 439, 888
Hsp92II CATG 3 cut(s) 502, 717, 840
HspAI GCGC 2 cut(s) 862, 882
Kzo9I GATC 2 cut(s) 297, 422
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 472, 654
Lsp1109I GCAGC 1 cut(s) 172
LweI GCATC 1 cut(s) 1031
MaeI CTAG 3 cut(s) 35, 551, 674
MaeII ACGT 2 cut(s) 439, 888
MaeIII GTNAC 7 cut(s) 172, 262, 501, 566, 921, 957, 1189
MalI GATC 2 cut(s) 299, 424
MboI GATC 2 cut(s) 297, 422
MhlI GDGCHC 2 cut(s) 311, 469
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 6 cut(s) 112, 626, 635, 753, 806, 830
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 6 cut(s) 180, 215, 221, 227, 326, 1039
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 6 cut(s) 86, 94, 105, 604, 1092, 1162
Mox20I TGGCCA 1 cut(s) 333
MroXI GAANNNNTTC 2 cut(s) 46, 1101
MscI TGGCCA 1 cut(s) 333
MseI TTAA 6 cut(s) 722, 801, 809, 998, 1134, 1213
Msp20I TGGCCA 1 cut(s) 333
MspA1I CMGCKG 2 cut(s) 428, 508
MspCI CTTAAG 1 cut(s) 1133
MspI CCGG 2 cut(s) 239, 1152
MspR9I CCNGG 3 cut(s) 792, 948, 1153
Mva1269I GAATGC 1 cut(s) 850
MvaI CCWGG 2 cut(s) 792, 948
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 7 cut(s) 64, 185, 330, 531, 646, 854, 881
NciI CCSGG 1 cut(s) 1153
NdeI CATATG 2 cut(s) 703, 1158
NdeII GATC 2 cut(s) 297, 422
NlaIII CATG 3 cut(s) 502, 717, 840
NlaIV GGNNCC 1 cut(s) 1150
NmuCI GTSAC 4 cut(s) 172, 262, 501, 566
NspI RCATGY 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 157
PctI GAATGC 1 cut(s) 850
PdmI GAANNNNTTC 2 cut(s) 46, 1101
PfeI GAWTC 1 cut(s) 813
PflMI CCANNNNNTGG 1 cut(s) 581
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 6 cut(s) 179, 215, 221, 227, 325, 1039
PpsI GAGTC 6 cut(s) 179, 215, 221, 227, 325, 1039
Ppu21I YACGTR 1 cut(s) 889
Psp6I CCWGG 2 cut(s) 790, 946
PspEI GGTNACC 1 cut(s) 921
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 2 cut(s) 790, 946
PspN4I GGNNCC 1 cut(s) 1150
PspPI GGNCC 3 cut(s) 944, 1126, 1176
PstI CTGCAG 2 cut(s) 190, 316
PstNI CAGNNNCTG 1 cut(s) 320
PvuII CAGCTG 2 cut(s) 428, 508
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 6 cut(s) 722, 801, 809, 998, 1134, 1213
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 297, 422
Sau96I GGNCC 3 cut(s) 944, 1126, 1176
SchI GAGTC 6 cut(s) 180, 215, 221, 227, 326, 1039
ScrFI CCNGG 3 cut(s) 792, 948, 1153
SduI GDGCHC 2 cut(s) 311, 469
SfaNI GCATC 1 cut(s) 1031
SfcI CTRYAG 4 cut(s) 186, 312, 487, 1089
SinI GGWCC 2 cut(s) 944, 1126
SmlI CTYRAG 1 cut(s) 1133
SmoI CTYRAG 1 cut(s) 1133
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 6 cut(s) 112, 626, 635, 753, 806, 830
SsiI CCGC 3 cut(s) 102, 652, 1124
SspMI CTAG 3 cut(s) 35, 551, 674
StyD4I CCNGG 3 cut(s) 790, 946, 1151
StyI CCWWGG 1 cut(s) 204
TaaI ACNGT 4 cut(s) 448, 464, 775, 798
TaiI ACGT 2 cut(s) 442, 891
TaqI TCGA 1 cut(s) 900
TasI AATT 6 cut(s) 112, 626, 635, 753, 806, 830
TfiI GAWTC 1 cut(s) 813
Tru1I TTAA 6 cut(s) 722, 801, 809, 998, 1134, 1213
Tru9I TTAA 6 cut(s) 722, 801, 809, 998, 1134, 1213
TscAI CASTG 6 cut(s) 187, 307, 536, 571, 774, 946
TseFI GTSAC 4 cut(s) 172, 262, 501, 566
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 4 cut(s) 172, 262, 501, 566
TspDTI ATGAA 4 cut(s) 17, 690, 768, 856
TspRI CASTG 6 cut(s) 187, 307, 536, 571, 774, 946
Van91I CCANNNNNTGG 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 1133
VpaK11BI GGWCC 2 cut(s) 944, 1126
XagI CCTNNNNNAGG 1 cut(s) 822
XapI RAATTY 2 cut(s) 626, 830
XceI RCATGY 1 cut(s) 840
XcmI CCANNNNNNNNNTGG 2 cut(s) 1010, 1170
XmiI GTMKAC 1 cut(s) 13
XmnI GAANNNNTTC 2 cut(s) 46, 1101
XspI CTAG 3 cut(s) 35, 551, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.