FvH4_6g30020

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007952
Physical Location & Seq
Forward (+)
23193447 .. 23197374
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30020.t2

Sequence Viewer

Length: 1221 bp
ATGCTCTCTCTGCGAACCAGTCGCAGATTTTGTCATGTGGTCGCTACTGCAGTGGTAGCTAGGAATCATTGTGCTGCTGCTGCTGCTGCTTCTGTGCCGAATTCGGTTTCCAATAATATAAAGGCATCGGAAGAACCTGCCCTGGTTAAGCTTAAAGCCGAGAGGGACCCTGAAAAGCTGTTTCAGCTGTTTAAGGCAAATTCCCACAACCGGCTTGTTATTGAGAACCGGTTTGCATTTGAAGACACGGTGGCTCGGTTAGCTGGTGCTCGCCGGTTTGATTACATTGAGCATTTGCTTGAGCATCAGAAGAGTCTTCCTCAAGGTCGGCGAGAAGGGTTTGTTATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAGCATGCTGTTGATACTTTTTGTGATATGCATTTATATGGTTGTTCTAGGACTGTTAAGTCATACAATGCTACACTTAAGGTTTTGACTCAAACTCGGGATTTAAGAGCTATTGAGGCGTTTATGAGTGATATTCCGCAGCAATTTGATATTCAATTGGACGTGTATTCGGTTAATATTGTTATCAAGGCTTTTTGTGAAATGGGGGATTTGACTAGAGCATATGTGATCATGGTAGAGATGGAAAAGTTGGGAATACGACCGGATGTCATTACATATACAACTCTGATATCAGCATTTTACAAATGTAGGAGGTGGGAGATTGCTAATGGTTTATGGAATCTTATGGTCTTGAAGAGGTGTTTACCTAATCTTGCAACTTTCAATGTTCGAATCCAATTTCTGGTTGATACGAAGCGAGCATGGGAAGCTAACAGAGTGATGAGGATGATGCAGAATATTGGAATAGCACCTGATGAAGTTACATTTAATTTGGTGATCAAAGGGTTTTGCCAGGCTGGGAAACTTGACATGGCAAAAAGGGTATATTCTTTTCTACATGACAAGGGATACAAGCCCAATGTTAAAATTTATCAGACCATGATTCATTATCTTTGCAGAGAAAGAGATTTTAATCTGGCATATACAATATGTAGAAATTGCATGCAGAAAAATTGGTATCCAAATGTGGATACTATTCGTACATTGCTTGAAGGCCTGAACAGGAATAGTCAGCTTGGTAAGGCTCAAGCAATCATGACGTTGGTTCGAAGAAGGGTGCCTCCATTTCCTTCAAAACAGTTGAGTTCTTTGCAATCCATACTAACCAAGAGTTGA

Protein Analysis

407

Amino Acids

46.67

Weight (kDa)

9.73

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 149 - 316 4.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 182 - 230 7.7e-13 PPR repeat family
PPR_3 PF13812 205 - 263 1.2e-10 Pentatricopeptide repeat domain
PPR_2 PF13041 216 - 261 3.1e-10 PPR repeat family
PPR_2 PF13041 255 - 300 4.2e-07 PPR repeat family
TPR_24 PF23276 272 - 377 2.3e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 274 - 331 9.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 283 - 310 1.9e-08 PPR repeat
PPR_2 PF13041 286 - 323 3.8e-08 PPR repeat family
PPR_2 PF13041 321 - 368 1.8e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 356
AasI GACNNNNNNGTC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 145
AccB1I GGYRCC 1 cut(s) 1162
AccB7I CCANNNNNTGG 1 cut(s) 787
AciI CCGC 1 cut(s) 521
AcsI RAATTY 3 cut(s) 100, 199, 972
AfaI GTAC 1 cut(s) 1087
AfiI CCNNNNNNNGG 2 cut(s) 210, 787
AflII CTTAAG 1 cut(s) 461
AflIII ACRYGT 1 cut(s) 546
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 6 cut(s) 242, 539, 739, 769, 1097, 1179
AhdI GACNNNNNGTC 1 cut(s) 650
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 141, 897
AluBI AGCT 8 cut(s) 59, 151, 178, 187, 263, 494, 815, 1120
AluI AGCT 8 cut(s) 59, 151, 178, 187, 263, 494, 815, 1120
Alw21I GWGCWC 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 1 cut(s) 1099
ApeKI GCWGC 6 cut(s) 74, 77, 80, 83, 86, 523
ApoI RAATTY 3 cut(s) 100, 199, 972
AsiGI ACCGGT 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 892
AsuII TTCGAA 2 cut(s) 775, 1153
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 1162
BbsI GAAGAC 2 cut(s) 249, 308
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 6 cut(s) 61, 64, 67, 70, 73, 535
BccI CCATC 1 cut(s) 619
BciT130I CCWGG 2 cut(s) 143, 899
BciVI GTATCC 3 cut(s) 947, 1069, 1074
BclI TGATCA 2 cut(s) 612, 882
BfaI CTAG 3 cut(s) 60, 432, 600
BfmI CTRYAG 1 cut(s) 48
BfrI CTTAAG 1 cut(s) 461
BfuAI ACCTGC 1 cut(s) 145
BfuI GTATCC 3 cut(s) 947, 1069, 1074
BisI GCNGC 6 cut(s) 75, 78, 81, 84, 87, 524
BlsI GCNGC 6 cut(s) 76, 79, 82, 85, 88, 525
Bme1390I CCNGG 2 cut(s) 143, 899
Bme18I GGWCC 1 cut(s) 166
BmeRI GACNNNNNGTC 1 cut(s) 650
BmeT110I CYCGRG 1 cut(s) 480
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 3 cut(s) 167, 168, 1164
BmrFI CCNGG 2 cut(s) 143, 899
BmsI GCATC 3 cut(s) 134, 313, 825
BpiI GAAGAC 2 cut(s) 249, 308
BplI GAGNNNNNCTC 2 cut(s) 304, 336
Bpu14I TTCGAA 2 cut(s) 775, 1153
BpuEI CTTGAG 3 cut(s) 306, 320, 1116
BsaBI GATNNNNATC 1 cut(s) 1017
BsaJI CCNNGG 1 cut(s) 141
BsaWI WCCGGW 2 cut(s) 228, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 210, 787
Bse118I RCCGGY 3 cut(s) 210, 228, 273
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 1088
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 2 cut(s) 143, 899
BseDI CCNNGG 1 cut(s) 141
BseGI GGATG 3 cut(s) 384, 655, 837
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 2 cut(s) 210, 787
BseMI GCAATG 1 cut(s) 1088
BseNI ACTGG 1 cut(s) 18
BseXI GCAGC 6 cut(s) 61, 64, 67, 70, 73, 535
BseYI CCCAGC 2 cut(s) 374, 902
Bsh1285I CGRYCG 1 cut(s) 647
BshFI GGCC 1 cut(s) 1101
BshNI GGYRCC 1 cut(s) 1162
BshTI ACCGGT 1 cut(s) 228
BsiEI CGRYCG 1 cut(s) 647
BsiHKAI GWGCWC 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 4 cut(s) 211, 229, 274, 647
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 2 cut(s) 210, 787
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 1 cut(s) 1101
BsoBI CYCGRG 1 cut(s) 480
Bsp119I TTCGAA 2 cut(s) 775, 1153
Bsp1286I GDGCHC 1 cut(s) 271
Bsp143I GATC 2 cut(s) 612, 882
BspACI CCGC 1 cut(s) 521
BspANI GGCC 1 cut(s) 1101
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 3 cut(s) 167, 168, 1164
BspMAI CTGCAG 1 cut(s) 52
BspMI ACCTGC 1 cut(s) 145
BspT104I TTCGAA 2 cut(s) 775, 1153
BspT107I GGYRCC 1 cut(s) 1162
BspTI CTTAAG 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 1088
BsrFI RCCGGY 3 cut(s) 210, 228, 273
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 3 cut(s) 210, 228, 273
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 2 cut(s) 612, 882
Bst2UI CCWGG 2 cut(s) 143, 899
Bst4CI ACNGT 3 cut(s) 250, 439, 1185
Bst6I CTCTTC 2 cut(s) 305, 734
BstAFI CTTAAG 1 cut(s) 461
BstBI TTCGAA 2 cut(s) 775, 1153
BstC8I GCNNGC 4 cut(s) 271, 390, 804, 1049
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 3 cut(s) 384, 655, 837
BstKTI GATC 2 cut(s) 615, 885
BstMBI GATC 2 cut(s) 612, 882
BstMCI CGRYCG 1 cut(s) 647
BstMWI GCNNNNNNNGC 9 cut(s) 10, 56, 80, 83, 86, 184, 260, 500, 812
BstNI CCWGG 2 cut(s) 143, 899
BstNSI RCATGY 2 cut(s) 392, 1051
BstSCI CCNGG 2 cut(s) 141, 897
BstSFI CTRYAG 1 cut(s) 48
BstV1I GCAGC 6 cut(s) 61, 64, 67, 70, 73, 535
BstV2I GAAGAC 2 cut(s) 249, 308
BsuI GTATCC 3 cut(s) 947, 1069, 1074
BsuRI GGCC 1 cut(s) 1101
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 384, 655, 837
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 4 cut(s) 271, 390, 804, 1049
CciI TCATGA 1 cut(s) 1140
Cfr10I RCCGGY 3 cut(s) 210, 228, 273
Cfr13I GGNCC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 1086
CspAI ACCGGT 1 cut(s) 228
CviAII CATG 9 cut(s) 35, 389, 616, 807, 916, 944, 985, 1048, 1141
CviQI GTAC 1 cut(s) 1086
DdeI CTNAG 1 cut(s) 384
DpnI GATC 2 cut(s) 614, 884
DpnII GATC 2 cut(s) 612, 882
DrdI GACNNNNNNGTC 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 2 cut(s) 305, 734
Eam1105I GACNNNNNGTC 1 cut(s) 650
EarI CTCTTC 2 cut(s) 305, 734
Eco147I AGGCCT 1 cut(s) 1101
Eco32I GATATC 1 cut(s) 675
Eco47I GGWCC 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 480
EcoO109I RGGNCCY 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 100
EcoRII CCWGG 2 cut(s) 141, 897
EcoRV GATATC 1 cut(s) 675
EcoT22I ATGCAT 1 cut(s) 417
FaeI CATG 9 cut(s) 38, 392, 619, 810, 919, 947, 988, 1051, 1144
FaqI GGGAC 1 cut(s) 179
FatI CATG 9 cut(s) 34, 388, 615, 806, 915, 943, 984, 1047, 1140
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 2 cut(s) 612, 882
Fnu4HI GCNGC 6 cut(s) 75, 78, 81, 84, 87, 524
FokI GGATG 3 cut(s) 391, 662, 844
Fsp4HI GCNGC 6 cut(s) 75, 78, 81, 84, 87, 524
FspBI CTAG 3 cut(s) 60, 432, 600
GluI GCNGC 6 cut(s) 75, 78, 81, 84, 87, 524
GsaI CCCAGC 2 cut(s) 378, 906
HaeIII GGCC 1 cut(s) 1101
HapII CCGG 4 cut(s) 211, 229, 274, 647
Hin1II CATG 9 cut(s) 38, 392, 619, 810, 919, 947, 988, 1051, 1144
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 6 cut(s) 64, 313, 472, 724, 777, 988
HpaII CCGG 4 cut(s) 211, 229, 274, 647
HphI GGTGA 1 cut(s) 892
Hpy166II GTNNAC 1 cut(s) 749
Hpy188I TCNGA 4 cut(s) 130, 309, 672, 981
Hpy188III TCNNGA 3 cut(s) 482, 736, 1141
Hpy8I GTNNAC 1 cut(s) 749
HpyAV CCTTC 4 cut(s) 329, 1091, 1152, 1185
HpyCH4III ACNGT 3 cut(s) 250, 439, 1185
HpyCH4IV ACGT 2 cut(s) 546, 1145
HpyCH4V TGCA 9 cut(s) 50, 236, 415, 761, 838, 1002, 1047, 1051, 1198
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 56, 80, 83, 86, 184, 260, 500, 812
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 2 cut(s) 546, 1145
Hsp92II CATG 9 cut(s) 38, 392, 619, 810, 919, 947, 988, 1051, 1144
KflI GGGWCCC 1 cut(s) 166
Ksp22I TGATCA 2 cut(s) 612, 882
Kzo9I GATC 2 cut(s) 612, 882
Lsp1109I GCAGC 6 cut(s) 61, 64, 67, 70, 73, 535
LweI GCATC 3 cut(s) 134, 313, 825
MaeI CTAG 3 cut(s) 60, 432, 600
MaeII ACGT 2 cut(s) 546, 1145
MaeIII GTNAC 1 cut(s) 865
MalI GATC 2 cut(s) 614, 884
MboI GATC 2 cut(s) 612, 882
MboII GAAGA 6 cut(s) 143, 254, 308, 322, 751, 1167
MfeI CAATTG 1 cut(s) 539
MhlI GDGCHC 1 cut(s) 271
MluCI AATT 9 cut(s) 100, 199, 527, 539, 782, 874, 972, 1042, 1057
MlyI GAGTC 2 cut(s) 322, 466
MnlI CCTC 8 cut(s) 156, 330, 342, 493, 690, 735, 822, 1176
Mph1103I ATGCAT 1 cut(s) 417
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 1 cut(s) 187
MspCI CTTAAG 1 cut(s) 461
MspI CCGG 4 cut(s) 211, 229, 274, 647
MspR9I CCNGG 2 cut(s) 143, 899
MunI CAATTG 1 cut(s) 539
MvaI CCWGG 2 cut(s) 143, 899
MwoI GCNNNNNNNGC 9 cut(s) 10, 56, 80, 83, 86, 184, 260, 500, 812
NdeI CATATG 1 cut(s) 607
NdeII GATC 2 cut(s) 612, 882
NlaIII CATG 9 cut(s) 38, 392, 619, 810, 919, 947, 988, 1051, 1144
NlaIV GGNNCC 3 cut(s) 167, 168, 1164
NmeAIII GCCGAG 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 417
NspI RCATGY 2 cut(s) 392, 1051
NspV TTCGAA 2 cut(s) 775, 1153
PaeI GCATGC 2 cut(s) 392, 1051
PagI TCATGA 1 cut(s) 1140
PceI AGGCCT 1 cut(s) 1101
PfeI GAWTC 4 cut(s) 64, 724, 777, 988
PflMI CCANNNNNTGG 1 cut(s) 787
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 6 cut(s) 76, 79, 82, 85, 88, 525
PleI GAGTC 2 cut(s) 321, 466
PpsI GAGTC 2 cut(s) 321, 466
PpuMI RGGWCCY 1 cut(s) 166
PsiI TTATAA 1 cut(s) 356
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 2 cut(s) 141, 897
PspFI CCCAGC 2 cut(s) 374, 902
PspGI CCWGG 2 cut(s) 141, 897
PspN4I GGNNCC 3 cut(s) 167, 168, 1164
PspPI GGNCC 1 cut(s) 166
PspPPI RGGWCCY 1 cut(s) 166
PstI CTGCAG 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 1 cut(s) 1087
RsaNI GTAC 1 cut(s) 1086
RseI CAYNNNNRTG 1 cut(s) 420
SatI GCNGC 6 cut(s) 75, 78, 81, 84, 87, 524
Sau3AI GATC 2 cut(s) 612, 882
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 2 cut(s) 322, 466
ScrFI CCNGG 2 cut(s) 143, 899
SduI GDGCHC 1 cut(s) 271
SfaNI GCATC 3 cut(s) 134, 313, 825
SfcI CTRYAG 1 cut(s) 48
SfuI TTCGAA 2 cut(s) 775, 1153
SinI GGWCC 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 4 cut(s) 299, 321, 461, 1131
SmoI CTYRAG 4 cut(s) 299, 321, 461, 1131
SphI GCATGC 2 cut(s) 392, 1051
Sse9I AATT 9 cut(s) 100, 199, 527, 539, 782, 874, 972, 1042, 1057
SseBI AGGCCT 1 cut(s) 1101
SsiI CCGC 1 cut(s) 521
SspI AATATT 2 cut(s) 562, 844
SspMI CTAG 3 cut(s) 60, 432, 600
StuI AGGCCT 1 cut(s) 1101
StyD4I CCNGG 2 cut(s) 141, 897
TaaI ACNGT 3 cut(s) 250, 439, 1185
TaiI ACGT 2 cut(s) 549, 1148
TaqI TCGA 2 cut(s) 775, 1153
TasI AATT 9 cut(s) 100, 199, 527, 539, 782, 874, 972, 1042, 1057
TfiI GAWTC 4 cut(s) 64, 724, 777, 988
TscAI CASTG 1 cut(s) 57
TseI GCWGC 6 cut(s) 74, 77, 80, 83, 86, 523
TspDTI ATGAA 2 cut(s) 876, 980
TspRI CASTG 1 cut(s) 57
Van91I CCANNNNNTGG 1 cut(s) 787
Vha464I CTTAAG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 166
XapI RAATTY 3 cut(s) 100, 199, 972
XceI RCATGY 2 cut(s) 392, 1051
XspI CTAG 3 cut(s) 60, 432, 600
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.