MD00G1100500.v1.1

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007952
Physical Location & Seq
Reverse (-)
21234195 .. 21235472
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1100500.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGCTCTTTCTGCGAACCAGTCGCAGATTCTATAATGCCGCCATTGCCATAACCTCGAATGATTTCCCTGCAGCTGTGTCCAATCGAGTTTTGAATACGAAACCATTAGAAGAGCCTACACTCGTTAAGCTTAAAGCCGAGAGAGACCCCGAAAAACTGTTTCATTTGTTTAAGGCGAACGCGCACAATAGACTTGTCATTGAGAACCGCTTTGCATTTGAAGACACTGTGTCTCGGCTTGCTGGAGCTTGCCGGTTTGATTACATTGAACATTTGCTTGAGCATCAGAAGAGTCTTCCACAAGGTCGGCGAGAAGGGTTTATTATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAGCATGCTATTGATAGTTTTTGTGATATGCATTTGTATGGTTGTTCTAGGACTGTTAAATCTTTCAATGCTGCACTCAAGGTTCTGACTCAAACCCGTGATTTAGGAGCTGTTGAGGCATTTCTGAGGGAAATTCCGGAGAAGTTTGACATTGAAGTGGATATATATTCGTTGAATATTGTTATCAAGGCTTTTTGCGAAATGGGTATTTTGGTTAAAGCATATCTGTTCATGGTACATATGGAGAAGTTGGGAATAAAACCAGACGTCATTACATACACAACACTGATGTCAGCATTTTATAAGGACAACCGGTGGGAAATTGGTAATGGTTTATGGAATCTTATGATACTGAAGGGGTGTTTGCCTAATCTTGCGACTTTCAATGTTAGAATCCAGTTTCTGGTACATAGGAGACGAGCCTGGGAAGCTAATAGATTGATGGGTTTGATGCAGAATATTGAGATGACACCTGATGAGGTTACATATAATTTGGTCATCAAAGGTTTTTGTCAGGCAGGGTATCTTGAAATGGCCAAAAGGGTGTATTCTGCTCTACATGTGAAGGGATACAACCCTAATGTCAAAATATATCAGACTATGATTCATTATCTTTGTAAAGGAGGGGATTTTGATTTGGCGTATACTATGTGTAAAGATTGCATGCAAAAGAATTGGTTTCCGAATGTGGATACCATTCATACATTGCTTGAAGGCCTAAAGAAGGCAAACCAGCTTGGTAAGGCTAAGGCAATCATGATGTTGGTTCAAAAAAGGGTGCCTCCATTTTCATCAAAACAATTGGGTGCTTTGCAAACCATACTGACCAAGAGTCGAAATAAGATGAAGGACTAG

Protein Analysis

406

Amino Acids

46.61

Weight (kDa)

9.48

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 175 - 223 1.6e-10 PPR repeat family
PPR_3 PF13812 201 - 256 2.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 209 - 254 1.6e-09 PPR repeat family
PPR_1 PF12854 277 - 303 3.4e-07 PPR repeat
PPR_2 PF13041 279 - 316 1.9e-07 PPR repeat family
PPR_2 PF13041 314 - 362 4.2e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 335, 666
AatII GACGTC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 766
AccI GTMKAC 1 cut(s) 1007
AccII CGCG 1 cut(s) 182
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 2 cut(s) 39, 208
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 630
AfaI GTAC 2 cut(s) 600, 771
AfiI CCNNNNNNNGG 2 cut(s) 766, 1087
AflIII ACRYGT 1 cut(s) 922
AgeI ACCGGT 1 cut(s) 675
AhdI GACNNNNNGTC 2 cut(s) 229, 1194
AjnI CCWGG 1 cut(s) 785
AluBI AGCT 6 cut(s) 74, 130, 248, 473, 794, 1099
AluI AGCT 6 cut(s) 74, 130, 248, 473, 794, 1099
Alw26I GTCTC 3 cut(s) 138, 237, 772
AlwNI CAGNNNCTG 1 cut(s) 766
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 2 cut(s) 897, 1078
ApeKI GCWGC 2 cut(s) 71, 434
ApoI RAATTY 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 675
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 1 cut(s) 184
BalI TGGCCA 1 cut(s) 899
BanI GGYRCC 1 cut(s) 1141
BbsI GAAGAC 2 cut(s) 228, 287
BbvI GCAGC 2 cut(s) 83, 421
BccI CCATC 1 cut(s) 799
BciT130I CCWGG 1 cut(s) 787
BciVI GTATCC 2 cut(s) 926, 1048
BcoDI GTCTC 3 cut(s) 138, 237, 772
BfaI CTAG 2 cut(s) 411, 1216
BfmI CTRYAG 1 cut(s) 69
BfuI GTATCC 2 cut(s) 926, 1048
BisI GCNGC 3 cut(s) 39, 72, 435
BlsI GCNGC 3 cut(s) 40, 73, 436
Bme1390I CCNGG 1 cut(s) 787
BmeRI GACNNNNNGTC 2 cut(s) 229, 1194
BmiI GGNNCC 1 cut(s) 1143
BmrFI CCNGG 1 cut(s) 787
BmsI GCATC 2 cut(s) 292, 804
BpiI GAAGAC 2 cut(s) 228, 287
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 299, 425
BsaHI GRCGYC 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 786
BsaWI WCCGGW 2 cut(s) 499, 675
Bsc4I CCNNNNNNNGG 2 cut(s) 766, 1087
Bse118I RCCGGY 2 cut(s) 252, 675
Bse1I ACTGG 2 cut(s) 18, 760
Bse3DI GCAATG 2 cut(s) 42, 1067
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 1 cut(s) 787
BseDI CCNNGG 1 cut(s) 786
BseGI GGATG 1 cut(s) 363
BseLI CCNNNNNNNGG 2 cut(s) 766, 1087
BseMI GCAATG 2 cut(s) 42, 1067
BseMII CTCAG 1 cut(s) 479
BseNI ACTGG 2 cut(s) 18, 760
BseXI GCAGC 2 cut(s) 83, 421
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 899, 1080
BshNI GGYRCC 1 cut(s) 1141
BshTI ACCGGT 1 cut(s) 675
BsiSI CCGG 3 cut(s) 253, 500, 676
BslI CCNNNNNNNGG 2 cut(s) 766, 1087
BsmAI GTCTC 3 cut(s) 138, 237, 772
BsmBI CGTCTC 1 cut(s) 772
BsnI GGCC 2 cut(s) 899, 1080
Bso31I GGTCTC 1 cut(s) 138
Bsp13I TCCGGA 1 cut(s) 499
BspACI CCGC 2 cut(s) 39, 208
BspANI GGCC 2 cut(s) 899, 1080
BspCNI CTCAG 1 cut(s) 480
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 1 cut(s) 182
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 1 cut(s) 1143
BspMAI CTGCAG 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 1141
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 2 cut(s) 42, 1067
BsrFI RCCGGY 2 cut(s) 252, 675
BsrI ACTGG 2 cut(s) 18, 760
BssAI RCCGGY 2 cut(s) 252, 675
BssECI CCNNGG 1 cut(s) 786
BssNAI GTATAC 1 cut(s) 1008
BssNI GRCGYC 1 cut(s) 630
Bst1107I GTATAC 1 cut(s) 1008
Bst2UI CCWGG 1 cut(s) 787
Bst4CI ACNGT 3 cut(s) 159, 229, 418
Bst6I CTCTTC 2 cut(s) 105, 284
BstACI GRCGYC 1 cut(s) 630
BstC8I GCNNGC 4 cut(s) 240, 250, 369, 1028
BstDEI CTNAG 3 cut(s) 363, 488, 1110
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstF5I GGATG 1 cut(s) 363
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 184
BstMAI GTCTC 3 cut(s) 138, 237, 772
BstMWI GCNNNNNNNGC 4 cut(s) 10, 44, 479, 791
BstNI CCWGG 1 cut(s) 787
BstNSI RCATGY 3 cut(s) 371, 926, 1030
BstSCI CCNGG 1 cut(s) 785
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 83, 421
BstV2I GAAGAC 2 cut(s) 228, 287
BstZ17I GTATAC 1 cut(s) 1008
BsuI GTATCC 2 cut(s) 926, 1048
BsuRI GGCC 2 cut(s) 899, 1080
BtsCI GGATG 1 cut(s) 363
BtsIMutI CAGTG 2 cut(s) 225, 647
Cac8I GCNNGC 4 cut(s) 240, 250, 369, 1028
CaiI CAGNNNCTG 1 cut(s) 766
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 2 cut(s) 252, 675
Csp6I GTAC 2 cut(s) 599, 770
CspAI ACCGGT 1 cut(s) 675
CviAII CATG 5 cut(s) 368, 595, 923, 1027, 1120
CviQI GTAC 2 cut(s) 599, 770
DdeI CTNAG 3 cut(s) 363, 488, 1110
DriI GACNNNNNGTC 2 cut(s) 229, 1194
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 2 cut(s) 105, 284
Eam1105I GACNNNNNGTC 2 cut(s) 229, 1194
EarI CTCTTC 2 cut(s) 105, 284
Eco147I AGGCCT 1 cut(s) 1080
Eco31I GGTCTC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 737
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoRII CCWGG 1 cut(s) 785
EcoT22I ATGCAT 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 772
FaeI CATG 5 cut(s) 371, 598, 926, 1030, 1123
FatI CATG 5 cut(s) 367, 594, 922, 1026, 1119
FauNDI CATATG 1 cut(s) 603
FblI GTMKAC 1 cut(s) 1007
Fnu4HI GCNGC 3 cut(s) 39, 72, 435
FokI GGATG 1 cut(s) 370
Fsp4HI GCNGC 3 cut(s) 39, 72, 435
FspBI CTAG 2 cut(s) 411, 1216
GlaI GCGC 1 cut(s) 183
GluI GCNGC 3 cut(s) 39, 72, 435
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 2 cut(s) 899, 1080
HapII CCGG 3 cut(s) 253, 500, 676
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 630
Hin1II CATG 5 cut(s) 371, 598, 926, 1030, 1123
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 7 cut(s) 27, 292, 451, 703, 756, 967, 1195
HpaII CCGG 3 cut(s) 253, 500, 676
Hpy166II GTNNAC 1 cut(s) 1008
Hpy188I TCNGA 5 cut(s) 288, 450, 489, 960, 1047
Hpy188III TCNNGA 3 cut(s) 500, 890, 1120
Hpy8I GTNNAC 1 cut(s) 1008
HpyAV CCTTC 6 cut(s) 308, 712, 922, 1070, 1081, 1204
HpyCH4III ACNGT 3 cut(s) 159, 229, 418
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 8 cut(s) 71, 215, 394, 437, 817, 1026, 1030, 1177
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 44, 479, 791
HpyF3I CTNAG 3 cut(s) 363, 488, 1110
HpySE526I ACGT 1 cut(s) 630
Hsp92I GRCGYC 1 cut(s) 630
Hsp92II CATG 5 cut(s) 371, 598, 926, 1030, 1123
HspAI GCGC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 499
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 2 cut(s) 245, 470
Lsp1109I GCAGC 2 cut(s) 83, 421
LweI GCATC 2 cut(s) 292, 804
MaeI CTAG 2 cut(s) 411, 1216
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 1 cut(s) 844
MboII GAAGA 4 cut(s) 122, 233, 287, 301
MfeI CAATTG 1 cut(s) 1163
MlsI TGGCCA 1 cut(s) 899
MluCI AATT 5 cut(s) 495, 684, 853, 1036, 1163
MluNI TGGCCA 1 cut(s) 899
MlyI GAGTC 3 cut(s) 301, 445, 1204
MnlI CCTC 7 cut(s) 64, 321, 472, 483, 835, 980, 1155
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 396
MroI TCCGGA 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 899
MseI TTAA 5 cut(s) 126, 132, 171, 420, 579
MslI CAYNNNNRTG 2 cut(s) 399, 518
Msp20I TGGCCA 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 3 cut(s) 253, 500, 676
MspR9I CCNGG 1 cut(s) 787
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 1 cut(s) 787
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 10, 44, 479, 791
NdeI CATATG 1 cut(s) 603
NlaIII CATG 5 cut(s) 371, 598, 926, 1030, 1123
NlaIV GGNNCC 1 cut(s) 1143
NmeAIII GCCGAG 2 cut(s) 163, 214
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 3 cut(s) 371, 926, 1030
PaeI GCATGC 2 cut(s) 371, 1030
PagI TCATGA 1 cut(s) 1119
PceI AGGCCT 1 cut(s) 1080
PciI ACATGT 1 cut(s) 922
PciSI GCTCTTC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 4 cut(s) 27, 703, 756, 967
PflMI CCANNNNNTGG 1 cut(s) 766
PinAI ACCGGT 1 cut(s) 675
PkrI GCNGC 3 cut(s) 40, 73, 436
PleI GAGTC 3 cut(s) 300, 445, 1203
PpsI GAGTC 3 cut(s) 300, 445, 1203
PscI ACATGT 1 cut(s) 922
PsiI TTATAA 2 cut(s) 335, 666
Psp6I CCWGG 1 cut(s) 785
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 1 cut(s) 785
PspN4I GGNNCC 1 cut(s) 1143
PstI CTGCAG 1 cut(s) 73
PstNI CAGNNNCTG 1 cut(s) 766
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 2 cut(s) 600, 771
RsaNI GTAC 2 cut(s) 599, 770
RseI CAYNNNNRTG 2 cut(s) 399, 518
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 5 cut(s) 126, 132, 171, 420, 579
SatI GCNGC 3 cut(s) 39, 72, 435
SchI GAGTC 3 cut(s) 301, 445, 1204
ScrFI CCNGG 1 cut(s) 787
SfaNI GCATC 2 cut(s) 292, 804
SfcI CTRYAG 1 cut(s) 69
SmiMI CAYNNNNRTG 2 cut(s) 399, 518
SmlI CTYRAG 2 cut(s) 278, 440
SmoI CTYRAG 2 cut(s) 278, 440
SphI GCATGC 2 cut(s) 371, 1030
Sse9I AATT 5 cut(s) 495, 684, 853, 1036, 1163
SseBI AGGCCT 1 cut(s) 1080
SsiI CCGC 2 cut(s) 39, 208
SspI AATATT 2 cut(s) 541, 823
SspMI CTAG 2 cut(s) 411, 1216
StuI AGGCCT 1 cut(s) 1080
StyD4I CCNGG 1 cut(s) 785
TaaI ACNGT 3 cut(s) 159, 229, 418
TaiI ACGT 1 cut(s) 633
TaqI TCGA 3 cut(s) 56, 85, 1198
TasI AATT 5 cut(s) 495, 684, 853, 1036, 1163
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 4 cut(s) 27, 703, 756, 967
Tru1I TTAA 5 cut(s) 126, 132, 171, 420, 579
Tru9I TTAA 5 cut(s) 126, 132, 171, 420, 579
TscAI CASTG 2 cut(s) 232, 654
TseI GCWGC 2 cut(s) 71, 434
TspDTI ATGAA 5 cut(s) 152, 583, 959, 1052, 1143
TspRI CASTG 2 cut(s) 232, 654
Van91I CCANNNNNTGG 1 cut(s) 766
XagI CCTNNNNNAGG 1 cut(s) 1085
XapI RAATTY 1 cut(s) 495
XceI RCATGY 3 cut(s) 371, 926, 1030
XmiI GTMKAC 1 cut(s) 1007
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 2 cut(s) 411, 1216
ZraI GACGTC 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.