pycom09g13930

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0007952
Physical Location & Seq
Reverse (-)
13099507 .. 13100706
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g13930.1

Sequence Viewer

Length: 1077 bp
ATGCTCTCTCTGCGAACCAGTCGCAGATTCTGTAATACCGCCATTGCCACAACCTCGAATGATTTCCCTGCAGCCGTGTCCAATCGAGTTTTGAATACGAAACCATTAGAAGAGCCTGCCCTGGTTAAGCTTAAAGCCGAGAGAGACCCCGAAAAACTGTTTCATTTGTTTAAGGCGAACGCGCACAATAGACTTGTCATTGAGAACCGCTTTGCATTTGAAGACACTGTGTCTCGTCTTGCTGGAGCTTGCCGGTTTGATTACATTGAACATTTGCTTGAGCATCAGAAGAGTCTTCCACAAGGTCGGCGGGAAGGGTTTATTATGAGGATTATAACGTTGTATGGAGCTGTTGAGGCATTTCTGAGGGAGATTCCAGAGAAGTTTGACATTGAAGTGGATATATATTCGGTGAATATTGTTATCAAGGCTTTTTGCGAAATGGGTATTTTGGTTAAAGCATATCTGATCATGGTACATATGGAGAAGTTGGGAATAAAACCAGACGTCTATACATACACAACACTGATGTCAGCATTTTATAAGGACAACCAGTGGGAAATTGGTAATGGTTTATGGAATCTTATGATACTGAAGGGGTGTTTGCCTAATCTTGCGACTTTCAATGTTAGAATCCAGTTTCTGGTACATAGGAGACGAGCATGGGAAGCTAATAGATTGATGGGTTTGATGCAGAATATTGAGATGACACCTGATGAGGTTACATATAATTTGGTCATCAAAGGTTTTTGTCAGGCAGGGTATCTTGAAATGGCCAAAAGGGTGTATTCTGCTCTACATGCAAAGGGATACAACCCTAATGTCAAAATATATCAGACTATGATTCATTATCTTTGTAAAGGAAGGGATTTTGATTTGGCGTATACTATGTGTAAAGATTGCATGCAAAAGAATTGGTTTCCGAATGTGGATACCATTCATACATTGCTTGAAGGCCTAAAGAAGGCAAACCAGCTTGGTAAGGCTAAGGCAATCATGATGTTGGTTCAAAAAAGGGTGCCTCCATTTTCATCAAAACAATTGGGTGCTTTGCAAACCATACTGACCAAGAGTTGA

Protein Analysis

359

Amino Acids

41.25

Weight (kDa)

9.35

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 134 - 182 3.4e-11 PPR repeat family
PPR_3 PF13812 157 - 215 2.3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 173 - 213 2.9e-06 PPR repeat family
PPR_1 PF12854 236 - 262 2.9e-07 PPR repeat
PPR_2 PF13041 238 - 275 1.2e-07 PPR repeat family
PPR_2 PF13041 273 - 321 1.6e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 335, 543
AatII GACGTC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 1018
AccB7I CCANNNNNTGG 1 cut(s) 643
AccI GTMKAC 1 cut(s) 884
AccII CGCG 1 cut(s) 182
AciI CCGC 3 cut(s) 39, 208, 310
AclI AACGTT 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 774
AcuI CTGAAG 1 cut(s) 614
AcyI GRCGYC 1 cut(s) 507
AfaI GTAC 2 cut(s) 477, 648
AfiI CCNNNNNNNGG 2 cut(s) 643, 964
AgsI TTSAA 8 cut(s) 94, 221, 269, 395, 625, 770, 953, 1010
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 120
AluBI AGCT 5 cut(s) 130, 248, 350, 671, 976
AluI AGCT 5 cut(s) 130, 248, 350, 671, 976
Alw26I GTCTC 3 cut(s) 138, 237, 649
AlwNI CAGNNNCTG 2 cut(s) 30, 643
AoxI GGCC 2 cut(s) 774, 955
ApeKI GCWGC 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 424
BalI TGGCCA 1 cut(s) 776
BanI GGYRCC 1 cut(s) 1018
BbsI GAAGAC 2 cut(s) 228, 287
BbvI GCAGC 1 cut(s) 83
BccI CCATC 1 cut(s) 676
BceAI ACGGC 1 cut(s) 59
BciT130I CCWGG 1 cut(s) 122
BciVI GTATCC 2 cut(s) 803, 925
BclI TGATCA 1 cut(s) 468
BcoDI GTCTC 3 cut(s) 138, 237, 649
BfmI CTRYAG 1 cut(s) 69
BfuI GTATCC 2 cut(s) 803, 925
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 1 cut(s) 122
BmeRI GACNNNNNGTC 1 cut(s) 229
BmiI GGNNCC 1 cut(s) 1020
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 2 cut(s) 292, 681
BpiI GAAGAC 2 cut(s) 228, 287
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 299
BsaHI GRCGYC 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 2 cut(s) 643, 964
Bse118I RCCGGY 1 cut(s) 252
Bse1I ACTGG 3 cut(s) 18, 553, 637
Bse3DI GCAATG 2 cut(s) 42, 944
BseBI CCWGG 1 cut(s) 122
BseDI CCNNGG 1 cut(s) 120
BseLI CCNNNNNNNGG 2 cut(s) 643, 964
BseMI GCAATG 2 cut(s) 42, 944
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 3 cut(s) 18, 553, 637
BseXI GCAGC 1 cut(s) 83
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 776, 957
BshNI GGYRCC 1 cut(s) 1018
BsiSI CCGG 1 cut(s) 253
BslI CCNNNNNNNGG 2 cut(s) 643, 964
BsmAI GTCTC 3 cut(s) 138, 237, 649
BsmBI CGTCTC 1 cut(s) 649
BsnI GGCC 2 cut(s) 776, 957
Bso31I GGTCTC 1 cut(s) 138
Bsp143I GATC 1 cut(s) 468
BspACI CCGC 3 cut(s) 39, 208, 310
BspANI GGCC 2 cut(s) 776, 957
BspCNI CTCAG 1 cut(s) 357
BspFNI CGCG 1 cut(s) 182
BspHI TCATGA 1 cut(s) 996
BspLI GGNNCC 1 cut(s) 1020
BspMAI CTGCAG 1 cut(s) 73
BspQI GCTCTTC 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 1018
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 2 cut(s) 42, 944
BsrFI RCCGGY 1 cut(s) 252
BsrI ACTGG 3 cut(s) 18, 553, 637
BssAI RCCGGY 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 1 cut(s) 468
BssNAI GTATAC 1 cut(s) 885
BssNI GRCGYC 1 cut(s) 507
Bst1107I GTATAC 1 cut(s) 885
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 159, 229
Bst6I CTCTTC 2 cut(s) 105, 284
BstACI GRCGYC 1 cut(s) 507
BstC8I GCNNGC 3 cut(s) 117, 250, 905
BstDEI CTNAG 2 cut(s) 365, 987
BstENI CCTNNNNNAGG 1 cut(s) 962
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 1 cut(s) 471
BstMAI GTCTC 3 cut(s) 138, 237, 649
BstMBI GATC 1 cut(s) 468
BstMWI GCNNNNNNNGC 4 cut(s) 10, 356, 668, 800
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 2 cut(s) 803, 907
BstSCI CCNGG 1 cut(s) 120
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 83
BstV2I GAAGAC 2 cut(s) 228, 287
BstZ17I GTATAC 1 cut(s) 885
BsuI GTATCC 2 cut(s) 803, 925
BsuRI GGCC 2 cut(s) 776, 957
BtsIMutI CAGTG 3 cut(s) 225, 524, 560
Cac8I GCNNGC 3 cut(s) 117, 250, 905
CaiI CAGNNNCTG 2 cut(s) 30, 643
CciI TCATGA 1 cut(s) 996
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 252
Csp6I GTAC 2 cut(s) 476, 647
CviAII CATG 5 cut(s) 472, 663, 800, 904, 997
CviQI GTAC 2 cut(s) 476, 647
DdeI CTNAG 2 cut(s) 365, 987
DpnI GATC 1 cut(s) 470
DpnII GATC 1 cut(s) 468
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 774
Eam1104I CTCTTC 2 cut(s) 105, 284
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 2 cut(s) 105, 284
Eco147I AGGCCT 1 cut(s) 957
Eco31I GGTCTC 1 cut(s) 138
Eco57I CTGAAG 1 cut(s) 614
EcoNI CCTNNNNNAGG 1 cut(s) 962
EcoRII CCWGG 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 649
FaeI CATG 5 cut(s) 475, 666, 803, 907, 1000
FatI CATG 5 cut(s) 471, 662, 799, 903, 996
FauI CCCGC 1 cut(s) 303
FauNDI CATATG 1 cut(s) 480
FbaI TGATCA 1 cut(s) 468
FblI GTMKAC 1 cut(s) 884
Fnu4HI GCNGC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 72
GlaI GCGC 1 cut(s) 183
GluI GCNGC 1 cut(s) 72
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 2 cut(s) 776, 957
HapII CCGG 1 cut(s) 253
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 5 cut(s) 475, 666, 803, 907, 1000
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 6 cut(s) 27, 292, 373, 580, 633, 844
HpaII CCGG 1 cut(s) 253
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 1 cut(s) 885
Hpy188I TCNGA 5 cut(s) 288, 366, 468, 837, 924
Hpy188III TCNNGA 3 cut(s) 377, 767, 997
Hpy8I GTNNAC 1 cut(s) 885
HpyAV CCTTC 5 cut(s) 308, 589, 858, 947, 958
HpyCH4III ACNGT 2 cut(s) 159, 229
HpyCH4IV ACGT 2 cut(s) 338, 507
HpyCH4V TGCA 7 cut(s) 71, 215, 694, 803, 903, 907, 1054
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 356, 668, 800
HpyF3I CTNAG 2 cut(s) 365, 987
HpySE526I ACGT 2 cut(s) 338, 507
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 5 cut(s) 475, 666, 803, 907, 1000
HspAI GCGC 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 468
Kzo9I GATC 1 cut(s) 468
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 2 cut(s) 245, 347
Lsp1109I GCAGC 1 cut(s) 83
LweI GCATC 2 cut(s) 292, 681
MaeII ACGT 2 cut(s) 338, 507
MaeIII GTNAC 1 cut(s) 721
MalI GATC 1 cut(s) 470
MboI GATC 1 cut(s) 468
MboII GAAGA 4 cut(s) 122, 233, 287, 301
MfeI CAATTG 1 cut(s) 1040
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 4 cut(s) 561, 730, 913, 1040
MluNI TGGCCA 1 cut(s) 776
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 6 cut(s) 64, 321, 349, 360, 712, 1032
Mox20I TGGCCA 1 cut(s) 776
MroXI GAANNNNTTC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 776
MseI TTAA 4 cut(s) 126, 132, 171, 456
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 776
MspI CCGG 1 cut(s) 253
MspR9I CCNGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 1040
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 10, 356, 668, 800
NdeI CATATG 1 cut(s) 480
NdeII GATC 1 cut(s) 468
NlaIII CATG 5 cut(s) 475, 666, 803, 907, 1000
NlaIV GGNNCC 1 cut(s) 1020
NmeAIII GCCGAG 1 cut(s) 163
NspI RCATGY 2 cut(s) 803, 907
PaeI GCATGC 1 cut(s) 907
PagI TCATGA 1 cut(s) 996
PceI AGGCCT 1 cut(s) 957
PciSI GCTCTTC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 5 cut(s) 27, 373, 580, 633, 844
PflMI CCANNNNNTGG 1 cut(s) 643
PkrI GCNGC 1 cut(s) 73
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PsiI TTATAA 2 cut(s) 335, 543
Psp1406I AACGTT 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 1020
PstI CTGCAG 1 cut(s) 73
PstNI CAGNNNCTG 2 cut(s) 30, 643
RsaI GTAC 2 cut(s) 477, 648
RsaNI GTAC 2 cut(s) 476, 647
RseI CAYNNNNRTG 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 4 cut(s) 126, 132, 171, 456
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 1 cut(s) 468
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 122
SfaNI GCATC 2 cut(s) 292, 681
SfcI CTRYAG 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SphI GCATGC 1 cut(s) 907
Sse9I AATT 4 cut(s) 561, 730, 913, 1040
SseBI AGGCCT 1 cut(s) 957
SsiI CCGC 3 cut(s) 39, 208, 310
SspI AATATT 2 cut(s) 418, 700
StuI AGGCCT 1 cut(s) 957
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 159, 229
TaiI ACGT 2 cut(s) 341, 510
TaqI TCGA 2 cut(s) 56, 85
TasI AATT 4 cut(s) 561, 730, 913, 1040
TfiI GAWTC 5 cut(s) 27, 373, 580, 633, 844
Tru1I TTAA 4 cut(s) 126, 132, 171, 456
Tru9I TTAA 4 cut(s) 126, 132, 171, 456
TscAI CASTG 3 cut(s) 232, 531, 560
TseI GCWGC 1 cut(s) 71
TspDTI ATGAA 4 cut(s) 152, 836, 929, 1020
TspRI CASTG 3 cut(s) 232, 531, 560
Van91I CCANNNNNTGG 1 cut(s) 643
XagI CCTNNNNNAGG 1 cut(s) 962
XceI RCATGY 2 cut(s) 803, 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmiI GTMKAC 1 cut(s) 884
XmnI GAANNNNTTC 1 cut(s) 62
ZraI GACGTC 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.