Rw2G030940

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007952
Physical Location & Seq
Forward (+)
50813434 .. 50814635
1202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030940.1

Sequence Viewer

Length: 1155 bp
ATGCTCTCTCTGCGAACCAGTCGCAGATTTTGTCATGTGGCCGCTAAGGTAGTAGCTATGAATGATTGTGCTGCAGCTGTGCCCAAGTTGGTTTGCAATACAAAGGCCTTGGAGGAACCTGCCCTAGTTAAGCTTAAAGCAGAGAGGGATCCCGAGAAGCTATTCCAGCTATTTAAGGCCAATGCCCACAATCGGCTTGTCATTGAGAACCGCTTTGCATTTGAAGACACGGTGGCTCGGTTAGCTGGAGCTCGCCGGTTTGATTACATTGAGCATTTGCTTGAGCATCAGAAGACTCTTCCTCAAGGTCGGCGAGAAGGGTTCATTATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAGCATGCTATTGATACTTTTTGTGATATGCATTTATATGGTTGTTCTAGGACTGTTAAGTCATACAATGCTACACTCAGTGATATTCCACAGCAATTTGACATTCAGTTGGACATATATTCAGTTAATATTGTTATCAAGGCTTTTTGTGAAATGGGGATTTTGACGAAAGCATATGTGATCATGGTAGAGATGGAAAAGTTGGGAATAAGACCGGATGTCATTACGTATACAACTCTGATATCAGCATTTTATAAACATAGGCGGTGGGAGATTGCTAATGGGTTATGGAATCTTATGGTCCTGAAGAGGTGTTTACCTAACCTTGCAACTTTCAATGTTCGAATCCAATTTTTGGTTAATACAAAGCGAGCTTGGGAAGCTAATAGAGTTTTGAAGATGATGCAGAACATTGGGATAGCACCTGATGAAGTTACATTTAATTTGGTGATCAAAGGGTTTTGCCAGGCTGGGAAACTTGAAATGGCAAAAAGGGTATATTCTGCTCTACATGACAAGGGATACAAGCCCAATGTTAAAATTTATCAGACCATGATTCATTATCTTTGTAGAGAAAGGGATTTTGATCTGGCATATACATTGTGTAGAGATTCCATGCAGAAAAATTGGTATCCAAATGTGGATACCATTCATACATTGCTAAAAGGCCTGCGGATGAAAAGTCAGCTTGGCAAGGCTAAGGCTATCATGACGTTGGTTCGTAGAAGGTTGCCTCCATTTCCTTCAAAACAGTTGAGTTCTTTGCAATCCATACTAACCAAGAGTTGA

Protein Analysis

384

Amino Acids

44.28

Weight (kDa)

9.76

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 160 - 208 8e-12 PPR repeat family
PPR_long PF17177 162 - 293 1.8e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 183 - 242 6.3e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 194 - 239 1.3e-10 PPR repeat family
PPR_2 PF13041 229 - 278 9.5e-09 PPR repeat family
TPR_24 PF23276 250 - 329 5.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 252 - 309 3.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 261 - 288 7.3e-08 PPR repeat
PPR_2 PF13041 264 - 301 1.7e-08 PPR repeat family
PPR_2 PF13041 299 - 346 2.3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 338, 621
AasI GACNNNNNNGTC 1 cut(s) 424
Acc36I ACCTGC 1 cut(s) 127
AccB7I CCANNNNNTGG 1 cut(s) 721
AccI GTMKAC 1 cut(s) 596
AciI CCGC 4 cut(s) 42, 211, 631, 1039
AclWI GGATC 2 cut(s) 143, 156
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 906
AcuI CTGAAG 1 cut(s) 692
AdeI CACNNNGTG 1 cut(s) 446
AfiI CCNNNNNNNGG 2 cut(s) 192, 721
AgsI TTSAA 5 cut(s) 224, 703, 763, 848, 1113
AhdI GACNNNNNGTC 1 cut(s) 584
AjnI CCWGG 1 cut(s) 831
Alw21I GWGCWC 1 cut(s) 253
AlwI GGATC 2 cut(s) 143, 156
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 4 cut(s) 39, 105, 177, 1033
ApeKI GCWGC 2 cut(s) 71, 74
ApoI RAATTY 1 cut(s) 906
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 1 cut(s) 667
AsuHPI GGTGA 1 cut(s) 826
AsuII TTCGAA 1 cut(s) 709
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 667
BaeGI GKGCMC 1 cut(s) 84
BamHI GGATCC 1 cut(s) 148
BanII GRGCYC 1 cut(s) 253
BbsI GAAGAC 2 cut(s) 231, 299
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 2 cut(s) 58, 86
BccI CCATC 1 cut(s) 553
BciT130I CCWGG 1 cut(s) 833
BciVI GTATCC 3 cut(s) 881, 1003, 1008
BclI TGATCA 2 cut(s) 546, 816
BfaI CTAG 2 cut(s) 125, 414
BfmI CTRYAG 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 127
BfuI GTATCC 3 cut(s) 881, 1003, 1008
BisI GCNGC 3 cut(s) 42, 72, 75
BlsI GCNGC 3 cut(s) 43, 73, 76
Bme1390I CCNGG 1 cut(s) 833
Bme18I GGWCC 1 cut(s) 667
BmeRI GACNNNNNGTC 1 cut(s) 584
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 2 cut(s) 117, 150
BmrFI CCNGG 1 cut(s) 833
BmsI GCATC 2 cut(s) 295, 759
BpiI GAAGAC 2 cut(s) 231, 299
BpmI CTGGAG 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 45, 1065
Bpu14I TTCGAA 1 cut(s) 709
BpuEI CTTGAG 2 cut(s) 288, 302
BsaAI YACGTR 1 cut(s) 594
BsaBI GATNNNNATC 1 cut(s) 951
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 721
Bse118I RCCGGY 1 cut(s) 255
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 1022
Bse8I GATNNNNATC 1 cut(s) 951
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 1 cut(s) 108
BseGI GGATG 3 cut(s) 366, 589, 1047
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 2 cut(s) 192, 721
BseMI GCAATG 1 cut(s) 1022
BseMII CTCAG 1 cut(s) 457
BseNI ACTGG 1 cut(s) 18
BseSI GKGCMC 1 cut(s) 84
BseXI GCAGC 2 cut(s) 58, 86
BseYI CCCAGC 2 cut(s) 356, 836
BshFI GGCC 4 cut(s) 41, 107, 179, 1035
BsiHKAI GWGCWC 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 2 cut(s) 256, 581
BslI CCNNNNNNNGG 2 cut(s) 192, 721
BsnI GGCC 4 cut(s) 41, 107, 179, 1035
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 709
Bsp1286I GDGCHC 2 cut(s) 84, 253
Bsp143I GATC 4 cut(s) 148, 546, 816, 952
BspACI CCGC 4 cut(s) 42, 211, 631, 1039
BspANI GGCC 4 cut(s) 41, 107, 179, 1035
BspCNI CTCAG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 1074
BspLI GGNNCC 2 cut(s) 117, 150
BspMAI CTGCAG 1 cut(s) 76
BspMI ACCTGC 1 cut(s) 127
BspPI GGATC 2 cut(s) 143, 156
BspT104I TTCGAA 1 cut(s) 709
BsrDI GCAATG 1 cut(s) 1022
BsrFI RCCGGY 1 cut(s) 255
BsrI ACTGG 1 cut(s) 18
BssAI RCCGGY 1 cut(s) 255
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 4 cut(s) 148, 546, 816, 952
BssNAI GTATAC 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 108
Bst1107I GTATAC 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 3 cut(s) 232, 421, 1119
Bst6I CTCTTC 2 cut(s) 303, 668
BstBAI YACGTR 1 cut(s) 594
BstBI TTCGAA 1 cut(s) 709
BstC8I GCNNGC 4 cut(s) 253, 372, 738, 1037
BstDEI CTNAG 4 cut(s) 45, 366, 443, 1065
BstF5I GGATG 3 cut(s) 366, 589, 1047
BstKTI GATC 4 cut(s) 151, 549, 819, 955
BstMBI GATC 4 cut(s) 148, 546, 816, 952
BstMWI GCNNNNNNNGC 4 cut(s) 10, 166, 242, 746
BstNI CCWGG 1 cut(s) 833
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 831
BstSFI CTRYAG 1 cut(s) 72
BstSLI GKGCMC 1 cut(s) 84
BstSNI TACGTA 1 cut(s) 594
BstV1I GCAGC 2 cut(s) 58, 86
BstV2I GAAGAC 2 cut(s) 231, 299
BstX2I RGATCY 1 cut(s) 148
BstYI RGATCY 1 cut(s) 148
BstZ17I GTATAC 1 cut(s) 597
BsuI GTATCC 3 cut(s) 881, 1003, 1008
BsuRI GGCC 4 cut(s) 41, 107, 179, 1035
BtsCI GGATG 3 cut(s) 366, 589, 1047
BtsIMutI CAGTG 1 cut(s) 451
BveI ACCTGC 1 cut(s) 127
Cac8I GCNNGC 4 cut(s) 253, 372, 738, 1037
CciI TCATGA 1 cut(s) 1074
Cfr10I RCCGGY 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 667
CviAII CATG 7 cut(s) 35, 371, 550, 878, 919, 982, 1075
DdeI CTNAG 4 cut(s) 45, 366, 443, 1065
DpnI GATC 4 cut(s) 150, 548, 818, 954
DpnII GATC 4 cut(s) 148, 546, 816, 952
DraIII CACNNNGTG 1 cut(s) 446
DrdI GACNNNNNNGTC 1 cut(s) 424
DriI GACNNNNNGTC 1 cut(s) 584
DseDI GACNNNNNNGTC 1 cut(s) 424
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 303, 668
Eam1105I GACNNNNNGTC 1 cut(s) 584
EarI CTCTTC 2 cut(s) 303, 668
Ecl136II GAGCTC 1 cut(s) 251
Eco105I TACGTA 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 2 cut(s) 107, 1035
Eco24I GRGCYC 1 cut(s) 253
Eco32I GATATC 1 cut(s) 609
Eco47I GGWCC 1 cut(s) 667
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 831
EcoRV GATATC 1 cut(s) 609
EcoT14I CCWWGG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 399
EcoT38I GRGCYC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 7 cut(s) 38, 374, 553, 881, 922, 985, 1078
FatI CATG 7 cut(s) 34, 370, 549, 877, 918, 981, 1074
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 2 cut(s) 546, 816
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 3 cut(s) 42, 72, 75
FokI GGATG 3 cut(s) 373, 596, 1054
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 3 cut(s) 42, 72, 75
FspBI CTAG 2 cut(s) 125, 414
GluI GCNGC 3 cut(s) 42, 72, 75
GsaI CCCAGC 2 cut(s) 360, 840
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 4 cut(s) 41, 107, 179, 1035
HapII CCGG 2 cut(s) 256, 581
Hin1II CATG 7 cut(s) 38, 374, 553, 881, 922, 985, 1078
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 5 cut(s) 295, 658, 711, 922, 977
HpaII CCGG 2 cut(s) 256, 581
HphI GGTGA 1 cut(s) 826
Hpy166II GTNNAC 2 cut(s) 597, 683
Hpy188I TCNGA 3 cut(s) 291, 606, 915
Hpy188III TCNNGA 3 cut(s) 152, 670, 1075
Hpy8I GTNNAC 2 cut(s) 597, 683
HpyAV CCTTC 3 cut(s) 311, 1086, 1119
HpyCH4III ACNGT 3 cut(s) 232, 421, 1119
HpyCH4IV ACGT 2 cut(s) 593, 1079
HpyCH4V TGCA 8 cut(s) 74, 96, 218, 397, 695, 772, 985, 1132
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 166, 242, 746
HpyF3I CTNAG 4 cut(s) 45, 366, 443, 1065
HpySE526I ACGT 2 cut(s) 593, 1079
Hsp92II CATG 7 cut(s) 38, 374, 553, 881, 922, 985, 1078
Ksp22I TGATCA 2 cut(s) 546, 816
Kzo9I GATC 4 cut(s) 148, 546, 816, 952
LmnI GCTCC 1 cut(s) 248
Lsp1109I GCAGC 2 cut(s) 58, 86
LweI GCATC 2 cut(s) 295, 759
MaeI CTAG 2 cut(s) 125, 414
MaeII ACGT 2 cut(s) 593, 1079
MaeIII GTNAC 1 cut(s) 799
MalI GATC 4 cut(s) 150, 548, 818, 954
MboI GATC 4 cut(s) 148, 546, 816, 952
MboII GAAGA 5 cut(s) 236, 290, 304, 685, 775
MflI RGATCY 1 cut(s) 148
MhlI GDGCHC 2 cut(s) 84, 253
MluCI AATT 5 cut(s) 461, 716, 808, 906, 991
MlyI GAGTC 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 456
MnlI CCTC 6 cut(s) 106, 138, 312, 324, 669, 1110
Mph1103I ATGCAT 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 161
MseI TTAA 8 cut(s) 129, 135, 174, 423, 492, 726, 807, 903
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 2 cut(s) 256, 581
MspR9I CCNGG 1 cut(s) 833
MvaI CCWGG 1 cut(s) 833
MwoI GCNNNNNNNGC 4 cut(s) 10, 166, 242, 746
NdeI CATATG 1 cut(s) 541
NdeII GATC 4 cut(s) 148, 546, 816, 952
NlaIII CATG 7 cut(s) 38, 374, 553, 881, 922, 985, 1078
NlaIV GGNNCC 2 cut(s) 117, 150
NsiI ATGCAT 1 cut(s) 399
NspI RCATGY 1 cut(s) 374
NspV TTCGAA 1 cut(s) 709
PaeI GCATGC 1 cut(s) 374
PagI TCATGA 1 cut(s) 1074
PceI AGGCCT 2 cut(s) 107, 1035
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 4 cut(s) 658, 711, 922, 977
PflMI CCANNNNNTGG 1 cut(s) 721
PkrI GCNGC 3 cut(s) 43, 73, 76
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 594
PsiI TTATAA 2 cut(s) 338, 621
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 831
PspFI CCCAGC 2 cut(s) 356, 836
PspGI CCWGG 1 cut(s) 831
PspN4I GGNNCC 2 cut(s) 117, 150
PspPI GGNCC 1 cut(s) 667
PstI CTGCAG 1 cut(s) 76
PsuI RGATCY 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 77
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 8 cut(s) 129, 135, 174, 423, 492, 726, 807, 903
SatI GCNGC 3 cut(s) 42, 72, 75
Sau3AI GATC 4 cut(s) 148, 546, 816, 952
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 1 cut(s) 833
SduI GDGCHC 2 cut(s) 84, 253
SfaNI GCATC 2 cut(s) 295, 759
SfcI CTRYAG 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 709
SinI GGWCC 1 cut(s) 667
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 2 cut(s) 281, 303
SmoI CTYRAG 2 cut(s) 281, 303
SnaBI TACGTA 1 cut(s) 594
SphI GCATGC 1 cut(s) 374
Sse9I AATT 5 cut(s) 461, 716, 808, 906, 991
SseBI AGGCCT 2 cut(s) 107, 1035
SsiI CCGC 4 cut(s) 42, 211, 631, 1039
SspI AATATT 1 cut(s) 496
SspMI CTAG 2 cut(s) 125, 414
SstI GAGCTC 1 cut(s) 253
StuI AGGCCT 2 cut(s) 107, 1035
StyD4I CCNGG 1 cut(s) 831
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 232, 421, 1119
TaiI ACGT 2 cut(s) 596, 1082
TaqI TCGA 1 cut(s) 709
TasI AATT 5 cut(s) 461, 716, 808, 906, 991
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 4 cut(s) 658, 711, 922, 977
Tru1I TTAA 8 cut(s) 129, 135, 174, 423, 492, 726, 807, 903
Tru9I TTAA 8 cut(s) 129, 135, 174, 423, 492, 726, 807, 903
TscAI CASTG 1 cut(s) 451
TseI GCWGC 2 cut(s) 71, 74
TspDTI ATGAA 6 cut(s) 74, 313, 810, 914, 1007, 1058
TspRI CASTG 1 cut(s) 451
Van91I CCANNNNNTGG 1 cut(s) 721
VpaK11BI GGWCC 1 cut(s) 667
XapI RAATTY 1 cut(s) 906
XceI RCATGY 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 2 cut(s) 125, 414
Zsp2I ATGCAT 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.