RLG00000019499

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007952
Physical Location & Seq
Forward (+)
52797914 .. 52799059
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019499

Sequence Viewer

Length: 1146 bp
ATGAATGATTGTGCTGCAGCTGTGCCCAAGTTGGTTTGCAATACAAAGGCCTTGGAGGAACCTGCCCTGGTTAAGCTTAAAGCAGAGAGGGACCCCGAGAAGCTATTCCAGCTATTTAAGGCCAATGCCTACAATCGGCTTGTCATTGAGAACCGGTTTGCATTTGAAGACACGGTGGCTCGGTTAGCTGGAGCTCGCCGGTTTGATTACATTGAGCATTTGCTTGAGCATCAGAAGACTCTTCCTCAAGGTCGGCGAGAAGGGTTCATTATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAGCATGCTATTGATACTTTTTGTGATATGCATTTATATGGATGTTCTAGGACTGTTAAGTCATACAATGCTACACTCAAGGTTTTGACTCAAACTCGTGATTTAAGAGCAATTGAGGCATTTGTGAGTGACATTCCACAGCAATTTGACATTCAGTTGGACGTATATTCAGTTAATATTGTTATCAAGGCTTTTTGTGAAATGGGGATTTTGACTAAAGCATATGTGATCATGGTAGAGATGGAAAAGTTGGGAATAAGACCTGATGTCATTACGTATACAACTCTGATATCAGCATTTTATAAACAGAGGCAGTGGGAGATTGCTAATGGGTTATGGAATCTTATGGTCCTGAAGAGGTGTTTACCTAATCTTGCAACTTTCAATGTTCGAATCCAATTTTTGGTTAATACAAAGCGAGCTTGGGAAGCTAATAGAGTTTTGAAGATGATGCAGAACATTGGGATAGCACCTGATGAAGTTACATTTAATTTGGTGATCAAAGGGTTTTGCCAGGCTGGGAAACTTGAAATGGCAAAAAGGGTATATTCTGCTCTACATGACAAGGGATACAAGCCCAATGTTAAAATTTATCAGACCATGATTCATTATCTTTGTAGAGAAAGGGATTTTGATCTGGCATATACATTGTGTAGAGATTCCATGCAGAAAAATTGGTATCCAAATGTGGATACCATTCATACATTGCTAAAAGGCCTGCGGATGAAAAGTCAGCTTGGCAAGGCTAAGGCTATCATGACATTGGTTCGTAGAAGGGTGCCTCCATTTCCTTCAAAACAGTTGAGTTCTTTGCAATCCATACTAACCAAGAGTTGA

Protein Analysis

382

Amino Acids

43.95

Weight (kDa)

9.62

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 124 - 290 7.8e-08 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 157 - 205 4.7e-12 PPR repeat family
PPR_3 PF13812 180 - 239 1.2e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 191 - 236 1e-10 PPR repeat family
PPR_2 PF13041 230 - 275 2e-07 PPR repeat family
TPR_24 PF23276 247 - 326 5.4e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 249 - 306 3.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 258 - 285 7.3e-08 PPR repeat
PPR_2 PF13041 261 - 298 1.4e-08 PPR repeat family
PPR_2 PF13041 296 - 343 2.3e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 281, 612
AasI GACNNNNNNGTC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 1087
AccB7I CCANNNNNTGG 1 cut(s) 712
AccI GTMKAC 1 cut(s) 587
AciI CCGC 1 cut(s) 1030
AcsI RAATTY 1 cut(s) 897
AcuI CTGAAG 1 cut(s) 683
AfiI CCNNNNNNNGG 2 cut(s) 135, 712
AgeI ACCGGT 1 cut(s) 153
AgsI TTSAA 5 cut(s) 167, 694, 754, 839, 1104
AhdI GACNNNNNGTC 1 cut(s) 575
AjnI CCWGG 2 cut(s) 66, 822
AluBI AGCT 9 cut(s) 20, 76, 103, 112, 188, 194, 731, 740, 1045
AluI AGCT 9 cut(s) 20, 76, 103, 112, 188, 194, 731, 740, 1045
Alw21I GWGCWC 1 cut(s) 196
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 3 cut(s) 48, 120, 1024
ApeKI GCWGC 2 cut(s) 14, 17
ApoI RAATTY 1 cut(s) 897
AsiGI ACCGGT 1 cut(s) 153
Asp700I GAANNNNTTC 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 91, 658
AsuHPI GGTGA 1 cut(s) 817
AsuII TTCGAA 1 cut(s) 700
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 2 cut(s) 91, 658
BaeGI GKGCMC 1 cut(s) 27
BanI GGYRCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 196
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 2 cut(s) 174, 242
Bbv12I GWGCWC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 29
BccI CCATC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 68, 824
BciVI GTATCC 3 cut(s) 872, 994, 999
BclI TGATCA 2 cut(s) 537, 807
BfaI CTAG 1 cut(s) 357
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 70
BfuI GTATCC 3 cut(s) 872, 994, 999
BisI GCNGC 2 cut(s) 15, 18
BlsI GCNGC 2 cut(s) 16, 19
Bme1390I CCNGG 2 cut(s) 68, 824
Bme18I GGWCC 2 cut(s) 91, 658
BmeRI GACNNNNNGTC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 2 cut(s) 91, 658
BmiI GGNNCC 4 cut(s) 60, 92, 93, 1089
BmrFI CCNGG 2 cut(s) 68, 824
BmsI GCATC 2 cut(s) 238, 750
BpiI GAAGAC 2 cut(s) 174, 242
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 1056
Bpu14I TTCGAA 1 cut(s) 700
BpuEI CTTGAG 3 cut(s) 231, 245, 371
BsaAI YACGTR 1 cut(s) 585
BsaBI GATNNNNATC 1 cut(s) 942
BsaJI CCNNGG 2 cut(s) 51, 66
BsaWI WCCGGW 1 cut(s) 153
Bsc4I CCNNNNNNNGG 2 cut(s) 135, 712
Bse118I RCCGGY 2 cut(s) 153, 198
Bse3DI GCAATG 1 cut(s) 1013
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 2 cut(s) 68, 824
BseDI CCNNGG 2 cut(s) 51, 66
BseGI GGATG 3 cut(s) 309, 356, 1038
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 2 cut(s) 135, 712
BseMI GCAATG 1 cut(s) 1013
BseSI GKGCMC 1 cut(s) 27
BseXI GCAGC 1 cut(s) 29
BseYI CCCAGC 2 cut(s) 299, 827
BshFI GGCC 3 cut(s) 50, 122, 1026
BshNI GGYRCC 1 cut(s) 1087
BshTI ACCGGT 1 cut(s) 153
BsiHKAI GWGCWC 1 cut(s) 196
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 154, 199
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 2 cut(s) 135, 712
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 3 cut(s) 50, 122, 1026
BsoBI CYCGRG 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 700
Bsp1286I GDGCHC 2 cut(s) 27, 196
Bsp143I GATC 3 cut(s) 537, 807, 943
BspACI CCGC 1 cut(s) 1030
BspANI GGCC 3 cut(s) 50, 122, 1026
BspHI TCATGA 1 cut(s) 1065
BspLI GGNNCC 4 cut(s) 60, 92, 93, 1089
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 70
BspT104I TTCGAA 1 cut(s) 700
BspT107I GGYRCC 1 cut(s) 1087
BsrDI GCAATG 1 cut(s) 1013
BsrFI RCCGGY 2 cut(s) 153, 198
BssAI RCCGGY 2 cut(s) 153, 198
BssECI CCNNGG 2 cut(s) 51, 66
BssMI GATC 3 cut(s) 537, 807, 943
BssNAI GTATAC 1 cut(s) 588
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 51
Bst1107I GTATAC 1 cut(s) 588
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 2 cut(s) 68, 824
Bst4CI ACNGT 3 cut(s) 175, 364, 1110
Bst6I CTCTTC 2 cut(s) 246, 659
BstBAI YACGTR 1 cut(s) 585
BstBI TTCGAA 1 cut(s) 700
BstC8I GCNNGC 4 cut(s) 196, 315, 729, 1028
BstDEI CTNAG 2 cut(s) 309, 1056
BstF5I GGATG 3 cut(s) 309, 356, 1038
BstKTI GATC 3 cut(s) 540, 810, 946
BstMBI GATC 3 cut(s) 537, 807, 943
BstMWI GCNNNNNNNGC 4 cut(s) 109, 185, 425, 737
BstNI CCWGG 2 cut(s) 68, 824
BstNSI RCATGY 1 cut(s) 317
BstSCI CCNGG 2 cut(s) 66, 822
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 27
BstSNI TACGTA 1 cut(s) 585
BstV1I GCAGC 1 cut(s) 29
BstV2I GAAGAC 2 cut(s) 174, 242
BstZ17I GTATAC 1 cut(s) 588
BsuI GTATCC 3 cut(s) 872, 994, 999
BsuRI GGCC 3 cut(s) 50, 122, 1026
BtsCI GGATG 3 cut(s) 309, 356, 1038
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 1 cut(s) 629
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 4 cut(s) 196, 315, 729, 1028
CciI TCATGA 1 cut(s) 1065
Cfr10I RCCGGY 2 cut(s) 153, 198
Cfr13I GGNCC 2 cut(s) 91, 658
CspAI ACCGGT 1 cut(s) 153
CviAII CATG 6 cut(s) 314, 541, 869, 910, 973, 1066
DdeI CTNAG 2 cut(s) 309, 1056
DpnI GATC 3 cut(s) 539, 809, 945
DpnII GATC 3 cut(s) 537, 807, 943
DrdI GACNNNNNNGTC 1 cut(s) 367
DriI GACNNNNNGTC 1 cut(s) 575
DseDI GACNNNNNNGTC 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 246, 659
Eam1105I GACNNNNNGTC 1 cut(s) 575
EarI CTCTTC 2 cut(s) 246, 659
Ecl136II GAGCTC 1 cut(s) 194
Eco105I TACGTA 1 cut(s) 585
Eco130I CCWWGG 1 cut(s) 51
Eco147I AGGCCT 2 cut(s) 50, 1026
Eco24I GRGCYC 1 cut(s) 196
Eco32I GATATC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 91, 658
Eco53kI GAGCTC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 683
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 66, 822
EcoRV GATATC 1 cut(s) 600
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 196
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 6 cut(s) 317, 544, 872, 913, 976, 1069
FaqI GGGAC 1 cut(s) 104
FatI CATG 6 cut(s) 313, 540, 868, 909, 972, 1065
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 2 cut(s) 537, 807
FblI GTMKAC 1 cut(s) 587
Fnu4HI GCNGC 2 cut(s) 15, 18
FokI GGATG 3 cut(s) 316, 363, 1045
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 2 cut(s) 15, 18
FspBI CTAG 1 cut(s) 357
GluI GCNGC 2 cut(s) 15, 18
GsaI CCCAGC 2 cut(s) 303, 831
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 3 cut(s) 50, 122, 1026
HapII CCGG 2 cut(s) 154, 199
Hin1II CATG 6 cut(s) 317, 544, 872, 913, 976, 1069
HindIII AAGCTT 1 cut(s) 74
HinfI GANTC 6 cut(s) 238, 397, 649, 702, 913, 968
HpaII CCGG 2 cut(s) 154, 199
HphI GGTGA 1 cut(s) 817
Hpy166II GTNNAC 2 cut(s) 588, 674
Hpy188I TCNGA 3 cut(s) 234, 597, 906
Hpy188III TCNNGA 3 cut(s) 407, 661, 1066
Hpy8I GTNNAC 2 cut(s) 588, 674
HpyAV CCTTC 3 cut(s) 254, 1077, 1110
HpyCH4III ACNGT 3 cut(s) 175, 364, 1110
HpyCH4IV ACGT 2 cut(s) 471, 584
HpyCH4V TGCA 8 cut(s) 17, 39, 161, 340, 686, 763, 976, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 185, 425, 737
HpyF3I CTNAG 2 cut(s) 309, 1056
HpySE526I ACGT 2 cut(s) 471, 584
Hsp92II CATG 6 cut(s) 317, 544, 872, 913, 976, 1069
KflI GGGWCCC 1 cut(s) 91
Ksp22I TGATCA 2 cut(s) 537, 807
Kzo9I GATC 3 cut(s) 537, 807, 943
LmnI GCTCC 1 cut(s) 191
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 2 cut(s) 238, 750
MaeI CTAG 1 cut(s) 357
MaeII ACGT 2 cut(s) 471, 584
MaeIII GTNAC 2 cut(s) 437, 790
MalI GATC 3 cut(s) 539, 809, 945
MboI GATC 3 cut(s) 537, 807, 943
MboII GAAGA 5 cut(s) 179, 233, 247, 676, 766
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 2 cut(s) 27, 196
MluCI AATT 6 cut(s) 420, 452, 707, 799, 897, 982
MlyI GAGTC 2 cut(s) 232, 391
MmeI TCCRAC 1 cut(s) 447
MnlI CCTC 8 cut(s) 49, 81, 255, 267, 418, 612, 660, 1101
Mph1103I ATGCAT 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 104
MseI TTAA 9 cut(s) 72, 78, 117, 366, 413, 483, 717, 798, 894
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 2 cut(s) 154, 199
MspR9I CCNGG 2 cut(s) 68, 824
MunI CAATTG 1 cut(s) 420
MvaI CCWGG 2 cut(s) 68, 824
MwoI GCNNNNNNNGC 4 cut(s) 109, 185, 425, 737
NdeI CATATG 1 cut(s) 532
NdeII GATC 3 cut(s) 537, 807, 943
NlaIII CATG 6 cut(s) 317, 544, 872, 913, 976, 1069
NlaIV GGNNCC 4 cut(s) 60, 92, 93, 1089
NmuCI GTSAC 1 cut(s) 437
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 1 cut(s) 317
NspV TTCGAA 1 cut(s) 700
PaeI GCATGC 1 cut(s) 317
PagI TCATGA 1 cut(s) 1065
PceI AGGCCT 2 cut(s) 50, 1026
PdmI GAANNNNTTC 1 cut(s) 104
PfeI GAWTC 4 cut(s) 649, 702, 913, 968
PflMI CCANNNNNTGG 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 153
PkrI GCNGC 2 cut(s) 16, 19
PleI GAGTC 2 cut(s) 232, 391
PpsI GAGTC 2 cut(s) 232, 391
Ppu21I YACGTR 1 cut(s) 585
PpuMI RGGWCCY 1 cut(s) 91
PsiI TTATAA 2 cut(s) 281, 612
Psp124BI GAGCTC 1 cut(s) 196
Psp5II RGGWCCY 1 cut(s) 91
Psp6I CCWGG 2 cut(s) 66, 822
PspFI CCCAGC 2 cut(s) 299, 827
PspGI CCWGG 2 cut(s) 66, 822
PspN4I GGNNCC 4 cut(s) 60, 92, 93, 1089
PspPI GGNCC 2 cut(s) 91, 658
PspPPI RGGWCCY 1 cut(s) 91
PstI CTGCAG 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 20
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 196
SaqAI TTAA 9 cut(s) 72, 78, 117, 366, 413, 483, 717, 798, 894
SatI GCNGC 2 cut(s) 15, 18
Sau3AI GATC 3 cut(s) 537, 807, 943
Sau96I GGNCC 2 cut(s) 91, 658
SchI GAGTC 2 cut(s) 232, 391
ScrFI CCNGG 2 cut(s) 68, 824
SduI GDGCHC 2 cut(s) 27, 196
SfaNI GCATC 2 cut(s) 238, 750
SfcI CTRYAG 1 cut(s) 15
SfuI TTCGAA 1 cut(s) 700
SinI GGWCC 2 cut(s) 91, 658
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 3 cut(s) 224, 246, 386
SmoI CTYRAG 3 cut(s) 224, 246, 386
SnaBI TACGTA 1 cut(s) 585
SphI GCATGC 1 cut(s) 317
Sse9I AATT 6 cut(s) 420, 452, 707, 799, 897, 982
SseBI AGGCCT 2 cut(s) 50, 1026
SsiI CCGC 1 cut(s) 1030
SspI AATATT 1 cut(s) 487
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 196
StuI AGGCCT 2 cut(s) 50, 1026
StyD4I CCNGG 2 cut(s) 66, 822
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 175, 364, 1110
TaiI ACGT 2 cut(s) 474, 587
TaqI TCGA 1 cut(s) 700
TasI AATT 6 cut(s) 420, 452, 707, 799, 897, 982
TfiI GAWTC 4 cut(s) 649, 702, 913, 968
Tru1I TTAA 9 cut(s) 72, 78, 117, 366, 413, 483, 717, 798, 894
Tru9I TTAA 9 cut(s) 72, 78, 117, 366, 413, 483, 717, 798, 894
TscAI CASTG 1 cut(s) 629
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 2 cut(s) 14, 17
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 6 cut(s) 17, 256, 801, 905, 998, 1049
TspRI CASTG 1 cut(s) 629
Van91I CCANNNNNTGG 1 cut(s) 712
VpaK11BI GGWCC 2 cut(s) 91, 658
XapI RAATTY 1 cut(s) 897
XceI RCATGY 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 104
XspI CTAG 1 cut(s) 357
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.