RchiOBHm_Chr2g0136051

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0007952
Physical Location & Seq
Forward (+)
53289629 .. 53294051
4423 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50688

Sequence Viewer

Length: 1206 bp
ATGGGTGGTGAAGCTTTTCTCAGTCGCAGATTTTGTCATGTGGCAGCTAAGGTAGTAGCTATGAATGATTGTGCTGCTGCTGTGCCCAATTTGGTTTGCAATACAAAGGCCTTGAAAGAACCTGCCCTGGTTAAGCTTAAAGCAGAGAGGGACCCCGAGAAGCTATTCCAGCTATTTAAGGCCAATGCCCACAATCGGCTTGTCATTGAGAATCGGTTTGCATTTGAAGACACGGTGGCTCGGTTAGCTGGAGCTCGCCGGTTTGATTACATTGAGCATTTGCTTGAGCATCAGAAGACTCTTCCTCAAGGTCGGCGAGAAGGGTTCATTATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAGCATGCTATTGATACTTTTTGTGATATGCATTTATATGGTTGTTCTAGGACTGTTAAGTCATACAATGCTACACTCAAGGTTTTGACTCAAACTCGTGATTTAAGAGCAATTGAGGCATTTGTGAGTGATATTCCACAGCAATTTGACATTCAGTTGGACATATATTCAGTTAATATTGTTATCAAGGCTTTTTGTGAAATGGGGATTTTGACGAAAGCATATGTGATCATGGTAGAGATGGAAAAGTTGGGAATAAGACCGGATGTCATTACGTATACAACTCTGATATCAGCATTTTATAAACATAGGCGGTGGGAGATTGCTAATGGGTTATGGAATCTTATGGTCCTGAAGAGGTGTTTACCTAATCTTGCAACTTTCAATGTTCGAATCCAATTTTTGGTTAATACAAAGCGAGCTTGGGAAGCTAATAGAGTTTTGAAGATGATGCAGAACATTGGGATAGCACCTGATGAAGTTACATTTAATTTGGTGATCAAAGGGTTTTGCCAGGCTGGGAAACTTGAAATGGCAAAAAGGGTATATTCTGCTCTACATGACAAGGGATACACGCCCAATGTTAAAATTTATCAGACCATGATTCATTATCTTTGTAGAGAAAGGGATTTCGATCTGGCATATACATTGTGTAGAGATTCCATGCTGAAAAATTGGTATCCAAATGTGGATACCATTCATACATTGCTAAAAGGCCTGCGGATGAAAAGTCAGCTTGGCAAGGCTAAGGCTATCATGACATTGGTTCGTAGAAGGGTGCCTCCATTTCCTTCAAAACAGTTGAGTTCTTTGCAATCCATACTAACCAAGAGTTGA

Protein Analysis

401

Amino Acids

46.05

Weight (kDa)

9.72

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 144 - 310 1.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 177 - 225 8.6e-12 PPR repeat family
PPR_3 PF13812 200 - 259 6.7e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 211 - 256 1.4e-10 PPR repeat family
PPR_2 PF13041 250 - 295 1.3e-07 PPR repeat family
TPR_24 PF23276 267 - 333 1e-05 Fungal tetratrico peptide repeats
PPR_3 PF13812 269 - 326 5.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 278 - 305 7.8e-08 PPR repeat
PPR_2 PF13041 281 - 318 4.9e-08 PPR repeat family
PPR_2 PF13041 316 - 363 1.5e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 341, 672
AasI GACNNNNNNGTC 1 cut(s) 427
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 1147
AccB7I CCANNNNNTGG 1 cut(s) 772
AccI GTMKAC 1 cut(s) 647
AciI CCGC 2 cut(s) 682, 1090
AcsI RAATTY 1 cut(s) 957
AcuI CTGAAG 1 cut(s) 743
AfiI CCNNNNNNNGG 2 cut(s) 195, 772
AgsI TTSAA 6 cut(s) 115, 227, 754, 814, 899, 1164
AhdI GACNNNNNGTC 1 cut(s) 635
AjnI CCWGG 2 cut(s) 126, 882
Alw21I GWGCWC 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 155
AoxI GGCC 3 cut(s) 108, 180, 1084
ApeKI GCWGC 3 cut(s) 44, 74, 77
ApoI RAATTY 1 cut(s) 957
Asp700I GAANNNNTTC 2 cut(s) 15, 164
AspS9I GGNCC 2 cut(s) 151, 718
AsuHPI GGTGA 2 cut(s) 20, 877
AsuII TTCGAA 1 cut(s) 760
AvaI CYCGRG 1 cut(s) 155
AvaII GGWCC 2 cut(s) 151, 718
BaeGI GKGCMC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 1147
BanII GRGCYC 1 cut(s) 256
BauI CACGAG 1 cut(s) 465
BbsI GAAGAC 2 cut(s) 234, 302
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 3 cut(s) 56, 61, 64
BccI CCATC 1 cut(s) 604
BciT130I CCWGG 2 cut(s) 128, 884
BciVI GTATCC 3 cut(s) 932, 1054, 1059
BclI TGATCA 2 cut(s) 597, 867
BfaI CTAG 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 3 cut(s) 932, 1054, 1059
BisI GCNGC 3 cut(s) 45, 75, 78
BlsI GCNGC 3 cut(s) 46, 76, 79
Bme1390I CCNGG 2 cut(s) 128, 884
Bme18I GGWCC 2 cut(s) 151, 718
BmeRI GACNNNNNGTC 1 cut(s) 635
BmeT110I CYCGRG 1 cut(s) 155
BmgT120I GGNCC 2 cut(s) 151, 718
BmiI GGNNCC 3 cut(s) 152, 153, 1149
BmrFI CCNGG 2 cut(s) 128, 884
BmsI GCATC 2 cut(s) 298, 810
BpiI GAAGAC 2 cut(s) 234, 302
BpmI CTGGAG 1 cut(s) 270
Bpu10I CCTNAGC 2 cut(s) 48, 1116
Bpu14I TTCGAA 1 cut(s) 760
BpuEI CTTGAG 3 cut(s) 291, 305, 431
BsaAI YACGTR 1 cut(s) 645
BsaBI GATNNNNATC 1 cut(s) 1002
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 631
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 772
Bse118I RCCGGY 1 cut(s) 258
Bse3DI GCAATG 1 cut(s) 1073
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 2 cut(s) 128, 884
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 3 cut(s) 369, 640, 1098
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 2 cut(s) 195, 772
BseMI GCAATG 1 cut(s) 1073
BseMII CTCAG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 3 cut(s) 56, 61, 64
BseYI CCCAGC 2 cut(s) 359, 887
BshFI GGCC 3 cut(s) 110, 182, 1086
BshNI GGYRCC 1 cut(s) 1147
BsiHKAI GWGCWC 1 cut(s) 256
BsiHKCI CYCGRG 1 cut(s) 155
BsiSI CCGG 2 cut(s) 259, 632
BslFI GGGAC 1 cut(s) 164
BslI CCNNNNNNNGG 2 cut(s) 195, 772
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 3 cut(s) 110, 182, 1086
BsoBI CYCGRG 1 cut(s) 155
Bsp119I TTCGAA 1 cut(s) 760
Bsp1286I GDGCHC 2 cut(s) 87, 256
Bsp143I GATC 3 cut(s) 597, 867, 1003
BspACI CCGC 2 cut(s) 682, 1090
BspANI GGCC 3 cut(s) 110, 182, 1086
BspCNI CTCAG 1 cut(s) 33
BspHI TCATGA 1 cut(s) 1125
BspLI GGNNCC 3 cut(s) 152, 153, 1149
BspMI ACCTGC 1 cut(s) 130
BspT104I TTCGAA 1 cut(s) 760
BspT107I GGYRCC 1 cut(s) 1147
BsrDI GCAATG 1 cut(s) 1073
BsrFI RCCGGY 1 cut(s) 258
BssAI RCCGGY 1 cut(s) 258
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 3 cut(s) 597, 867, 1003
BssNAI GTATAC 1 cut(s) 648
BssSI CACGAG 1 cut(s) 465
Bst1107I GTATAC 1 cut(s) 648
Bst2BI CACGAG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 128, 884
Bst4CI ACNGT 3 cut(s) 235, 424, 1170
Bst6I CTCTTC 2 cut(s) 306, 719
BstBAI YACGTR 1 cut(s) 645
BstBI TTCGAA 1 cut(s) 760
BstC8I GCNNGC 4 cut(s) 256, 375, 789, 1088
BstDEI CTNAG 4 cut(s) 20, 48, 369, 1116
BstF5I GGATG 3 cut(s) 369, 640, 1098
BstKTI GATC 3 cut(s) 600, 870, 1006
BstMBI GATC 3 cut(s) 597, 867, 1003
BstMWI GCNNNNNNNGC 4 cut(s) 169, 245, 485, 797
BstNI CCWGG 2 cut(s) 128, 884
BstNSI RCATGY 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 126, 882
BstSLI GKGCMC 1 cut(s) 87
BstSNI TACGTA 1 cut(s) 645
BstV1I GCAGC 3 cut(s) 56, 61, 64
BstV2I GAAGAC 2 cut(s) 234, 302
BstZ17I GTATAC 1 cut(s) 648
BsuI GTATCC 3 cut(s) 932, 1054, 1059
BsuRI GGCC 3 cut(s) 110, 182, 1086
BtsCI GGATG 3 cut(s) 369, 640, 1098
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 4 cut(s) 256, 375, 789, 1088
CciI TCATGA 1 cut(s) 1125
Cfr10I RCCGGY 1 cut(s) 258
Cfr13I GGNCC 2 cut(s) 151, 718
CviAII CATG 7 cut(s) 38, 374, 601, 929, 970, 1033, 1126
DdeI CTNAG 4 cut(s) 20, 48, 369, 1116
DpnI GATC 3 cut(s) 599, 869, 1005
DpnII GATC 3 cut(s) 597, 867, 1003
DrdI GACNNNNNNGTC 1 cut(s) 427
DriI GACNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 427
Eam1104I CTCTTC 2 cut(s) 306, 719
Eam1105I GACNNNNNGTC 1 cut(s) 635
EarI CTCTTC 2 cut(s) 306, 719
Ecl136II GAGCTC 1 cut(s) 254
Eco105I TACGTA 1 cut(s) 645
Eco147I AGGCCT 2 cut(s) 110, 1086
Eco24I GRGCYC 1 cut(s) 256
Eco32I GATATC 1 cut(s) 660
Eco47I GGWCC 2 cut(s) 151, 718
Eco53kI GAGCTC 1 cut(s) 254
Eco57I CTGAAG 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 155
EcoICRI GAGCTC 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 126, 882
EcoRV GATATC 1 cut(s) 660
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 256
FaeI CATG 7 cut(s) 41, 377, 604, 932, 973, 1036, 1129
FaqI GGGAC 1 cut(s) 164
FatI CATG 7 cut(s) 37, 373, 600, 928, 969, 1032, 1125
FauNDI CATATG 1 cut(s) 592
FbaI TGATCA 2 cut(s) 597, 867
FblI GTMKAC 1 cut(s) 647
Fnu4HI GCNGC 3 cut(s) 45, 75, 78
FokI GGATG 3 cut(s) 376, 647, 1105
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 3 cut(s) 45, 75, 78
FspBI CTAG 1 cut(s) 417
GluI GCNGC 3 cut(s) 45, 75, 78
GsaI CCCAGC 2 cut(s) 363, 891
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 3 cut(s) 110, 182, 1086
HapII CCGG 2 cut(s) 259, 632
Hin1II CATG 7 cut(s) 41, 377, 604, 932, 973, 1036, 1129
HindIII AAGCTT 2 cut(s) 12, 134
HinfI GANTC 7 cut(s) 211, 298, 457, 709, 762, 973, 1028
HpaII CCGG 2 cut(s) 259, 632
HphI GGTGA 2 cut(s) 20, 877
Hpy166II GTNNAC 2 cut(s) 648, 734
Hpy188I TCNGA 3 cut(s) 294, 657, 966
Hpy188III TCNNGA 3 cut(s) 467, 721, 1126
Hpy8I GTNNAC 2 cut(s) 648, 734
HpyAV CCTTC 3 cut(s) 314, 1137, 1170
HpyCH4III ACNGT 3 cut(s) 235, 424, 1170
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 6 cut(s) 99, 221, 400, 746, 823, 1183
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 245, 485, 797
HpyF3I CTNAG 4 cut(s) 20, 48, 369, 1116
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 7 cut(s) 41, 377, 604, 932, 973, 1036, 1129
KflI GGGWCCC 1 cut(s) 151
Ksp22I TGATCA 2 cut(s) 597, 867
Kzo9I GATC 3 cut(s) 597, 867, 1003
LmnI GCTCC 1 cut(s) 251
Lsp1109I GCAGC 3 cut(s) 56, 61, 64
LweI GCATC 2 cut(s) 298, 810
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 1 cut(s) 850
MalI GATC 3 cut(s) 599, 869, 1005
MboI GATC 3 cut(s) 597, 867, 1003
MboII GAAGA 5 cut(s) 239, 293, 307, 736, 826
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 2 cut(s) 87, 256
MluCI AATT 7 cut(s) 88, 480, 512, 767, 859, 957, 1042
MlyI GAGTC 2 cut(s) 292, 451
MmeI TCCRAC 1 cut(s) 507
MnlI CCTC 6 cut(s) 141, 315, 327, 478, 720, 1161
Mph1103I ATGCAT 1 cut(s) 402
MroXI GAANNNNTTC 2 cut(s) 15, 164
MseI TTAA 9 cut(s) 132, 138, 177, 426, 473, 543, 777, 858, 954
MslI CAYNNNNRTG 1 cut(s) 405
MspI CCGG 2 cut(s) 259, 632
MspR9I CCNGG 2 cut(s) 128, 884
MunI CAATTG 1 cut(s) 480
MvaI CCWGG 2 cut(s) 128, 884
MwoI GCNNNNNNNGC 4 cut(s) 169, 245, 485, 797
NdeI CATATG 1 cut(s) 592
NdeII GATC 3 cut(s) 597, 867, 1003
NlaIII CATG 7 cut(s) 41, 377, 604, 932, 973, 1036, 1129
NlaIV GGNNCC 3 cut(s) 152, 153, 1149
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 1 cut(s) 377
NspV TTCGAA 1 cut(s) 760
PaeI GCATGC 1 cut(s) 377
PagI TCATGA 1 cut(s) 1125
PceI AGGCCT 2 cut(s) 110, 1086
PdmI GAANNNNTTC 2 cut(s) 15, 164
PfeI GAWTC 5 cut(s) 211, 709, 762, 973, 1028
PflMI CCANNNNNTGG 1 cut(s) 772
PkrI GCNGC 3 cut(s) 46, 76, 79
PleI GAGTC 2 cut(s) 292, 451
PpsI GAGTC 2 cut(s) 292, 451
Ppu21I YACGTR 1 cut(s) 645
PpuMI RGGWCCY 1 cut(s) 151
PsiI TTATAA 2 cut(s) 341, 672
Psp124BI GAGCTC 1 cut(s) 256
Psp5II RGGWCCY 1 cut(s) 151
Psp6I CCWGG 2 cut(s) 126, 882
PspFI CCCAGC 2 cut(s) 359, 887
PspGI CCWGG 2 cut(s) 126, 882
PspN4I GGNNCC 3 cut(s) 152, 153, 1149
PspPI GGNCC 2 cut(s) 151, 718
PspPPI RGGWCCY 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 405
SacI GAGCTC 1 cut(s) 256
SaqAI TTAA 9 cut(s) 132, 138, 177, 426, 473, 543, 777, 858, 954
SatI GCNGC 3 cut(s) 45, 75, 78
Sau3AI GATC 3 cut(s) 597, 867, 1003
Sau96I GGNCC 2 cut(s) 151, 718
SchI GAGTC 2 cut(s) 292, 451
ScrFI CCNGG 2 cut(s) 128, 884
SduI GDGCHC 2 cut(s) 87, 256
SfaNI GCATC 2 cut(s) 298, 810
SfuI TTCGAA 1 cut(s) 760
SinI GGWCC 2 cut(s) 151, 718
SmiMI CAYNNNNRTG 1 cut(s) 405
SmlI CTYRAG 3 cut(s) 284, 306, 446
SmoI CTYRAG 3 cut(s) 284, 306, 446
SnaBI TACGTA 1 cut(s) 645
SphI GCATGC 1 cut(s) 377
Sse9I AATT 7 cut(s) 88, 480, 512, 767, 859, 957, 1042
SseBI AGGCCT 2 cut(s) 110, 1086
SsiI CCGC 2 cut(s) 682, 1090
SspI AATATT 1 cut(s) 547
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 1 cut(s) 256
StuI AGGCCT 2 cut(s) 110, 1086
StyD4I CCNGG 2 cut(s) 126, 882
TaaI ACNGT 3 cut(s) 235, 424, 1170
TaiI ACGT 1 cut(s) 647
TaqI TCGA 2 cut(s) 760, 1002
TasI AATT 7 cut(s) 88, 480, 512, 767, 859, 957, 1042
TfiI GAWTC 5 cut(s) 211, 709, 762, 973, 1028
Tru1I TTAA 9 cut(s) 132, 138, 177, 426, 473, 543, 777, 858, 954
Tru9I TTAA 9 cut(s) 132, 138, 177, 426, 473, 543, 777, 858, 954
TseI GCWGC 3 cut(s) 44, 74, 77
TspDTI ATGAA 6 cut(s) 77, 316, 861, 965, 1058, 1109
Van91I CCANNNNNTGG 1 cut(s) 772
VpaK11BI GGWCC 2 cut(s) 151, 718
XapI RAATTY 1 cut(s) 957
XceI RCATGY 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 647
XmnI GAANNNNTTC 2 cut(s) 15, 164
XspI CTAG 1 cut(s) 417
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.