MD17G1208400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007952
Physical Location & Seq
Reverse (-)
25117736 .. 25119432
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1208400.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGCTCTCTCTGCGAACCAGTCGCAGATTCTGTAATGCCGCCATTGCCATAACCTCGAATGATTGCCCTGCAGCTGCGTCCAATCGCGTTTTGAATACGAAACCATTAGAAGAGCCTGCCCTGGTTAAGCTTAAAGCTGAGAGAGACCCGGAAAAGCTGTTTCATTTGTTTAAGGCCAACGCGCACAATAGGCTTGTCATTGAGAACCGGTTTGCATTTGACGACATGGTGTCTCGACTTGCTGGAGCTCACCGGTTTGATTACATTGAACATTTGCTTGAGCATCAGAAGAGCCTTCCCCAAGGTCGGCGAGAAGGGTTTATAATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAACATGCTATTGATACTTTTTGTGATATGCATTTATATGGTTGTTCTAGGACTGTTAAATCTTTCAACGCTGCACTCAAGGTTCTGACTCAAACCCGTGATTTAGGAGCCCTAGAGGCATTTTTGAGTGAGATTCCGGAGAAGTTTGACATTGAACTGGATATATATTCGGTCAATATTGTCATAAAGGCTTTTTGCGAAATGGGTATTTTGGTTAAAGCATATCAGATCATGGTACAGATGGAGAAGTTGGGGATAAAGCCAGACGTCATTACATACACAACACTGATGTCAGCATTTTATAAGGACAACCGGTGGGAAATTGGTAATGGTTTGTGGAATCTTATGATATTGAAGGGCTGTTTGCCTAATCTTGCGACTTTCAATGTTAGAATCCAGTATCTGGTATATAGGAGACGAGCCTGGGAAGCGAATAGATTGATGGGTTTGATGCAGAATATTGAGATAACACCCGATGAGGTTACATATAATTTGGTCATCAAAGGTTTTTGTCAGGCGGGGTATCTTGAAATGGCCAAAAGGGTGTATTCTGCTCTACATGGAAAGGGATACAAGCCCAATGTCAAAATATATCAGACCATGATTCATTATCTTTGTAAAGGAGGAGATTTTGATTTGGCATATACTATGTGTAAAGATTGCATGCAAAAGAATTGGTTTCCGAATGTGGATACCATTCGTACATTGCTTGAAGGCCTAAAGAAGGCAAACCAGCTTGGTAAGGCTAAGGCAATCATGATGTTAGTTCGAAAAAGGGCGCCTCCATTTTCTACGAAACAATTGGGTGTTTTGCAAACCATACTGACCAAGAGTTGA

Protein Analysis

400

Amino Acids

45.65

Weight (kDa)

9.45

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 171 - 221 3.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 175 - 223 8.8e-12 PPR repeat family
PPR_3 PF13812 199 - 254 1.7e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 209 - 253 1.8e-09 PPR repeat family
PPR_2 PF13041 244 - 293 4.3e-08 PPR repeat family
PPR_1 PF12854 277 - 303 2.6e-07 PPR repeat
PPR_2 PF13041 279 - 316 8.4e-08 PPR repeat family
PPR_2 PF13041 314 - 362 1.5e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 323, 335, 666
AatII GACGTC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 766
AccII CGCG 2 cut(s) 87, 182
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 2 cut(s) 39, 881
AcoI YGGCCR 1 cut(s) 897
AcyI GRCGYC 2 cut(s) 630, 1142
AfaI GTAC 2 cut(s) 600, 1066
AfiI CCNNNNNNNGG 3 cut(s) 306, 766, 1087
AgeI ACCGGT 3 cut(s) 207, 252, 675
AgsI TTSAA 8 cut(s) 94, 269, 430, 518, 718, 748, 893, 1076
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 2 cut(s) 120, 785
AluBI AGCT 6 cut(s) 74, 130, 137, 157, 248, 1099
AluI AGCT 6 cut(s) 74, 130, 137, 157, 248, 1099
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 3 cut(s) 138, 237, 772
AlwNI CAGNNNCTG 2 cut(s) 30, 766
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 3 cut(s) 174, 897, 1078
ApeKI GCWGC 3 cut(s) 71, 74, 434
AsiGI ACCGGT 3 cut(s) 207, 252, 675
AspLEI GCGC 2 cut(s) 184, 1144
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 1132
BalI TGGCCA 1 cut(s) 899
BanI GGYRCC 1 cut(s) 1141
BanII GRGCYC 2 cut(s) 250, 475
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 3 cut(s) 61, 83, 421
BccI CCATC 2 cut(s) 598, 799
BciT130I CCWGG 2 cut(s) 122, 787
BciVI GTATCC 2 cut(s) 926, 1048
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 3 cut(s) 138, 237, 772
BfaI CTAG 2 cut(s) 411, 476
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 1145
BfuI GTATCC 2 cut(s) 926, 1048
BglI GCCNNNNNGGC 1 cut(s) 479
BisI GCNGC 4 cut(s) 39, 72, 75, 435
BlsI GCNGC 4 cut(s) 40, 73, 76, 436
Bme1390I CCNGG 3 cut(s) 122, 149, 787
BmeRI GACNNNNNGTC 1 cut(s) 229
BmiI GGNNCC 2 cut(s) 472, 1143
BmrFI CCNGG 3 cut(s) 122, 149, 787
BmsI GCATC 2 cut(s) 292, 804
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 1 cut(s) 1132
BpuEI CTTGAG 2 cut(s) 299, 425
BpuMI CCSGG 1 cut(s) 149
BsaHI GRCGYC 2 cut(s) 630, 1142
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 120, 301, 786
BsaWI WCCGGW 4 cut(s) 207, 252, 499, 675
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 766, 1087
Bse118I RCCGGY 3 cut(s) 207, 252, 675
Bse1I ACTGG 3 cut(s) 18, 525, 760
Bse3DI GCAATG 2 cut(s) 42, 1067
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 2 cut(s) 122, 787
BseDI CCNNGG 3 cut(s) 120, 301, 786
BseGI GGATG 1 cut(s) 363
BseLI CCNNNNNNNGG 3 cut(s) 306, 766, 1087
BseMI GCAATG 2 cut(s) 42, 1067
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 3 cut(s) 18, 525, 760
BseRI GAGGAG 1 cut(s) 1002
BseXI GCAGC 3 cut(s) 61, 83, 421
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 87, 182
BshFI GGCC 3 cut(s) 176, 899, 1080
BshNI GGYRCC 1 cut(s) 1141
BshTI ACCGGT 3 cut(s) 207, 252, 675
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 5 cut(s) 149, 208, 253, 500, 676
BslI CCNNNNNNNGG 3 cut(s) 306, 766, 1087
BsmAI GTCTC 3 cut(s) 138, 237, 772
BsmBI CGTCTC 1 cut(s) 772
BsnI GGCC 3 cut(s) 176, 899, 1080
Bso31I GGTCTC 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 1132
Bsp1286I GDGCHC 2 cut(s) 250, 475
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 1 cut(s) 591
BspACI CCGC 2 cut(s) 39, 881
BspANI GGCC 3 cut(s) 176, 899, 1080
BspCNI CTCAG 1 cut(s) 130
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 2 cut(s) 87, 182
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 2 cut(s) 472, 1143
BspMAI CTGCAG 1 cut(s) 73
BspQI GCTCTTC 2 cut(s) 105, 284
BspT104I TTCGAA 1 cut(s) 1132
BspT107I GGYRCC 1 cut(s) 1141
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 2 cut(s) 42, 1067
BsrFI RCCGGY 3 cut(s) 207, 252, 675
BsrI ACTGG 3 cut(s) 18, 525, 760
BssAI RCCGGY 3 cut(s) 207, 252, 675
BssECI CCNNGG 3 cut(s) 120, 301, 786
BssMI GATC 1 cut(s) 591
BssNI GRCGYC 2 cut(s) 630, 1142
BssT1I CCWWGG 1 cut(s) 301
Bst2UI CCWGG 2 cut(s) 122, 787
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 2 cut(s) 105, 284
BstACI GRCGYC 2 cut(s) 630, 1142
BstBI TTCGAA 1 cut(s) 1132
BstC8I GCNNGC 2 cut(s) 117, 1028
BstDEI CTNAG 2 cut(s) 138, 1110
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstF5I GGATG 1 cut(s) 363
BstFNI CGCG 2 cut(s) 87, 182
BstH2I RGCGCY 1 cut(s) 1145
BstHHI GCGC 2 cut(s) 184, 1144
BstKTI GATC 1 cut(s) 594
BstMAI GTCTC 3 cut(s) 138, 237, 772
BstMBI GATC 1 cut(s) 591
BstMWI GCNNNNNNNGC 5 cut(s) 10, 44, 190, 479, 791
BstNI CCWGG 2 cut(s) 122, 787
BstNSI RCATGY 2 cut(s) 371, 1030
BstSCI CCNGG 3 cut(s) 120, 147, 785
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 2 cut(s) 87, 182
BstV1I GCAGC 3 cut(s) 61, 83, 421
BsuI GTATCC 2 cut(s) 926, 1048
BsuRI GGCC 3 cut(s) 176, 899, 1080
BtsCI GGATG 1 cut(s) 363
BtsIMutI CAGTG 1 cut(s) 647
Cac8I GCNNGC 2 cut(s) 117, 1028
CaiI CAGNNNCTG 2 cut(s) 30, 766
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 2 cut(s) 184, 1144
Cfr10I RCCGGY 3 cut(s) 207, 252, 675
CseI GACGC 1 cut(s) 66
Csp6I GTAC 2 cut(s) 599, 1065
CspAI ACCGGT 3 cut(s) 207, 252, 675
CviAII CATG 7 cut(s) 226, 368, 595, 923, 964, 1027, 1120
CviQI GTAC 2 cut(s) 599, 1065
DdeI CTNAG 2 cut(s) 138, 1110
DinI GGCGCC 1 cut(s) 1143
DpnI GATC 1 cut(s) 593
DpnII GATC 1 cut(s) 591
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 2 cut(s) 105, 284
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 2 cut(s) 105, 284
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 301
Eco147I AGGCCT 1 cut(s) 1080
Eco24I GRGCYC 2 cut(s) 250, 475
Eco31I GGTCTC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoRII CCWGG 2 cut(s) 120, 785
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 2 cut(s) 250, 475
EgeI GGCGCC 1 cut(s) 1143
EheI GGCGCC 1 cut(s) 1143
ErhI CCWWGG 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 772
FaeI CATG 7 cut(s) 229, 371, 598, 926, 967, 1030, 1123
FatI CATG 7 cut(s) 225, 367, 594, 922, 963, 1026, 1119
FauI CCCGC 1 cut(s) 874
Fnu4HI GCNGC 4 cut(s) 39, 72, 75, 435
FokI GGATG 1 cut(s) 370
FriOI GRGCYC 2 cut(s) 250, 475
Fsp4HI GCNGC 4 cut(s) 39, 72, 75, 435
FspBI CTAG 2 cut(s) 411, 476
GlaI GCGC 2 cut(s) 183, 1143
GluI GCNGC 4 cut(s) 39, 72, 75, 435
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 264
HaeII RGCGCY 1 cut(s) 1145
HaeIII GGCC 3 cut(s) 176, 899, 1080
HapII CCGG 5 cut(s) 149, 208, 253, 500, 676
HgaI GACGC 1 cut(s) 66
HhaI GCGC 2 cut(s) 184, 1144
Hin1I GRCGYC 2 cut(s) 630, 1142
Hin1II CATG 7 cut(s) 229, 371, 598, 926, 967, 1030, 1123
Hin6I GCGC 2 cut(s) 182, 1142
HinP1I GCGC 2 cut(s) 182, 1142
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 6 cut(s) 27, 451, 496, 703, 756, 967
HpaII CCGG 5 cut(s) 149, 208, 253, 500, 676
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 5 cut(s) 288, 450, 591, 960, 1047
Hpy188III TCNNGA 4 cut(s) 234, 500, 890, 1120
HpyAV CCTTC 5 cut(s) 305, 308, 712, 1070, 1081
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 8 cut(s) 71, 215, 394, 437, 817, 1026, 1030, 1177
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 44, 190, 479, 791
HpyF3I CTNAG 2 cut(s) 138, 1110
HpySE526I ACGT 1 cut(s) 630
Hsp92I GRCGYC 2 cut(s) 630, 1142
Hsp92II CATG 7 cut(s) 229, 371, 598, 926, 967, 1030, 1123
HspAI GCGC 2 cut(s) 182, 1142
KasI GGCGCC 1 cut(s) 1141
Kpn2I TCCGGA 1 cut(s) 499
Kzo9I GATC 1 cut(s) 591
LguI GCTCTTC 2 cut(s) 105, 284
LmnI GCTCC 2 cut(s) 245, 470
Lsp1109I GCAGC 3 cut(s) 61, 83, 421
LweI GCATC 2 cut(s) 292, 804
MaeI CTAG 2 cut(s) 411, 476
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 1 cut(s) 844
MalI GATC 1 cut(s) 593
MboI GATC 1 cut(s) 591
MboII GAAGA 2 cut(s) 122, 301
MfeI CAATTG 1 cut(s) 1163
MhlI GDGCHC 2 cut(s) 250, 475
MlsI TGGCCA 1 cut(s) 899
MluCI AATT 4 cut(s) 684, 853, 1036, 1163
MluNI TGGCCA 1 cut(s) 899
Mly113I GGCGCC 1 cut(s) 1142
MlyI GAGTC 1 cut(s) 445
MnlI CCTC 6 cut(s) 64, 321, 472, 835, 980, 1155
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 396
MroI TCCGGA 1 cut(s) 499
MscI TGGCCA 1 cut(s) 899
MseI TTAA 5 cut(s) 126, 132, 171, 420, 579
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 5 cut(s) 149, 208, 253, 500, 676
MspR9I CCNGG 3 cut(s) 122, 149, 787
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 2 cut(s) 122, 787
MvnI CGCG 2 cut(s) 87, 182
MwoI GCNNNNNNNGC 5 cut(s) 10, 44, 190, 479, 791
NarI GGCGCC 1 cut(s) 1142
NciI CCSGG 1 cut(s) 149
NdeII GATC 1 cut(s) 591
NlaIII CATG 7 cut(s) 229, 371, 598, 926, 967, 1030, 1123
NlaIV GGNNCC 2 cut(s) 472, 1143
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 2 cut(s) 371, 1030
NspV TTCGAA 1 cut(s) 1132
PaeI GCATGC 1 cut(s) 1030
PagI TCATGA 1 cut(s) 1119
PceI AGGCCT 1 cut(s) 1080
PciSI GCTCTTC 2 cut(s) 105, 284
PfeI GAWTC 5 cut(s) 27, 496, 703, 756, 967
PflMI CCANNNNNTGG 1 cut(s) 766
PinAI ACCGGT 3 cut(s) 207, 252, 675
PkrI GCNGC 4 cut(s) 40, 73, 76, 436
PleI GAGTC 1 cut(s) 445
PluTI GGCGCC 1 cut(s) 1145
PpsI GAGTC 1 cut(s) 445
PsiI TTATAA 3 cut(s) 323, 335, 666
Psp124BI GAGCTC 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 120, 785
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 2 cut(s) 120, 785
PspN4I GGNNCC 2 cut(s) 472, 1143
PstI CTGCAG 1 cut(s) 73
PstNI CAGNNNCTG 2 cut(s) 30, 766
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 2 cut(s) 600, 1066
RsaNI GTAC 2 cut(s) 599, 1065
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 250
SapI GCTCTTC 2 cut(s) 105, 284
SaqAI TTAA 5 cut(s) 126, 132, 171, 420, 579
SatI GCNGC 4 cut(s) 39, 72, 75, 435
Sau3AI GATC 1 cut(s) 591
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 3 cut(s) 122, 149, 787
SduI GDGCHC 2 cut(s) 250, 475
SfaNI GCATC 2 cut(s) 292, 804
SfcI CTRYAG 1 cut(s) 69
SfoI GGCGCC 1 cut(s) 1143
SfuI TTCGAA 1 cut(s) 1132
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 278, 440
SmoI CTYRAG 2 cut(s) 278, 440
SphI GCATGC 1 cut(s) 1030
Sse9I AATT 4 cut(s) 684, 853, 1036, 1163
SseBI AGGCCT 1 cut(s) 1080
SsiI CCGC 2 cut(s) 39, 881
SspDI GGCGCC 1 cut(s) 1141
SspI AATATT 2 cut(s) 541, 823
SspMI CTAG 2 cut(s) 411, 476
SstI GAGCTC 1 cut(s) 250
StuI AGGCCT 1 cut(s) 1080
StyD4I CCNGG 3 cut(s) 120, 147, 785
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 1 cut(s) 633
TaqI TCGA 3 cut(s) 56, 235, 1132
TaqII GACCGA 1 cut(s) 523
TasI AATT 4 cut(s) 684, 853, 1036, 1163
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 5 cut(s) 27, 496, 703, 756, 967
Tru1I TTAA 5 cut(s) 126, 132, 171, 420, 579
Tru9I TTAA 5 cut(s) 126, 132, 171, 420, 579
TscAI CASTG 1 cut(s) 654
TseI GCWGC 3 cut(s) 71, 74, 434
TspDTI ATGAA 2 cut(s) 152, 959
TspRI CASTG 1 cut(s) 654
Van91I CCANNNNNTGG 1 cut(s) 766
XagI CCTNNNNNAGG 1 cut(s) 1085
XceI RCATGY 2 cut(s) 371, 1030
XspI CTAG 2 cut(s) 411, 476
ZraI GACGTC 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.