pycom17g21110

Pentatricopeptide repeat-containing protein At1g80150

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007952
Physical Location & Seq
Reverse (-)
19600047 .. 19601246
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g21110.1

Sequence Viewer

Length: 1200 bp
ATGCTCTCTCTGCGAACAAGTCGCAGATTCTGTAATGCCGCCATTGCCATAACCTCGAATGATTGCCCTGCAGCTGCGTCCAATCGCGTTTTGAATACGAAACCGTTGGAAGAGCCTGCCCTGGTTAAGCTTAAAGCTGAGAGAGACCCGGAAAAGCTGTTTCATTTGTTTAAGGCCAACGCGCACAATAGGCTTGTCATTGAGAACCGGTTTGCATTTGAAGACATGGTGTCTCGGCTTGCTGGAGCTCACCGGTTTGATTACATTGAACATTTGCTTGAGCATCAGAAGAGCCTTCCCCAAGGTCGGCGAGAAGGGTTTATAATGAGGATTATAACATTGTATGGTAAGGCTGGGATGACTAAGCATGCTATTGATACTTTTTGTGATATGCATTTATATGGTTGTTCTAGGACTGTTAAATCTTTCAACACTGCACTCAAGGTTCTGACTCAAACCCGTGATTTAGGAGCCATAGAGGCATTTTTGAGTGAGATTCCGGAGAAGTTTGACATTGAACTGGATACATATTCGGTCAATATTGTCATAAAGGCTTTTTGCGAAATAGGTATTTTGGTTAAAGCATATCAGATAATGGTACAGATGGAGAAGTTGGGGATAAAACCAGACGTCATTACATACACAACACTGATGTCAGCATTTTATAAGGACAACCGGTGGGAAATTGGTAATGGTTTGTGGAATCTTATGATATTGAAGGGCTGTTTGCCTAATCTTGCGACTTTCAATGTTAGAATCCAGTATCTGGTATATAGGAGACGAGCCTGGGAAGCGAATAGATTGATGGGTTTGATGCAGAATATTGAGATAACACCCGATGAGGTTACCTATAATTTGGTCATCAAAGGTTTTTGTCAGGCGGGGTATCTTGAAATGGCCAAAAGGGTGTATTCTGCTCTACATGGAAAGGGATACAAGCCCAATGTCAAAATTTATCAGACCATGATTCATTATCTTTGTAAAGGAGGAGATTTTGAGTTGGCATATACTATGTGTAAAGATTGCATGCAAAAGAATTGGTTTCCGAATGTGGATACCATTCATACATTGCTTGAAGGCCTAAAGAAGGCAAACCAGCTTGGTAAGGCTAAGGCAATCATGGTGTTGGTTCGAAAAAGGGCGCCTCCATTTTCTTCAAAACAATTGGGTGCTTTGCAAACCATACTGACCAAGAGTTGA

Protein Analysis

400

Amino Acids

45.59

Weight (kDa)

9.41

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 171 - 221 4.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 175 - 223 1.8e-11 PPR repeat family
PPR_3 PF13812 198 - 254 1.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 209 - 253 1.8e-09 PPR repeat family
PPR_2 PF13041 244 - 293 4.3e-08 PPR repeat family
PPR_1 PF12854 277 - 303 2.6e-07 PPR repeat
PPR_2 PF13041 279 - 316 8.4e-08 PPR repeat family
PPR_2 PF13041 314 - 362 3.1e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 323, 335, 666
AatII GACGTC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 766
AccII CGCG 2 cut(s) 87, 182
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 2 cut(s) 39, 881
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 1 cut(s) 951
AcyI GRCGYC 2 cut(s) 630, 1142
AfaI GTAC 1 cut(s) 600
AfiI CCNNNNNNNGG 3 cut(s) 306, 766, 1087
AgeI ACCGGT 3 cut(s) 207, 252, 675
AhdI GACNNNNNGTC 1 cut(s) 229
AjnI CCWGG 2 cut(s) 120, 785
AluBI AGCT 6 cut(s) 74, 130, 137, 157, 248, 1099
AluI AGCT 6 cut(s) 74, 130, 137, 157, 248, 1099
Alw21I GWGCWC 1 cut(s) 250
Alw26I GTCTC 3 cut(s) 138, 237, 772
AlwNI CAGNNNCTG 2 cut(s) 30, 766
Aor13HI TCCGGA 1 cut(s) 499
AoxI GGCC 3 cut(s) 174, 897, 1078
ApeKI GCWGC 2 cut(s) 71, 74
ApoI RAATTY 1 cut(s) 951
AsiGI ACCGGT 3 cut(s) 207, 252, 675
AspLEI GCGC 2 cut(s) 184, 1144
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 1132
BalI TGGCCA 1 cut(s) 899
BanI GGYRCC 1 cut(s) 1141
BanII GRGCYC 1 cut(s) 250
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 2 cut(s) 61, 83
BccI CCATC 2 cut(s) 598, 799
BciT130I CCWGG 2 cut(s) 122, 787
BciVI GTATCC 3 cut(s) 517, 926, 1048
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 3 cut(s) 138, 237, 772
BfaI CTAG 1 cut(s) 411
BfmI CTRYAG 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 1145
BfuI GTATCC 3 cut(s) 517, 926, 1048
BglI GCCNNNNNGGC 1 cut(s) 479
BisI GCNGC 3 cut(s) 39, 72, 75
BlsI GCNGC 3 cut(s) 40, 73, 76
Bme1390I CCNGG 3 cut(s) 122, 149, 787
BmeRI GACNNNNNGTC 1 cut(s) 229
BmiI GGNNCC 2 cut(s) 472, 1143
BmrFI CCNGG 3 cut(s) 122, 149, 787
BmsI GCATC 2 cut(s) 292, 804
BpiI GAAGAC 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 1110
Bpu14I TTCGAA 1 cut(s) 1132
BpuEI CTTGAG 2 cut(s) 299, 425
BpuMI CCSGG 1 cut(s) 149
BsaHI GRCGYC 2 cut(s) 630, 1142
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 120, 301, 786
BsaWI WCCGGW 4 cut(s) 207, 252, 499, 675
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 766, 1087
Bse118I RCCGGY 3 cut(s) 207, 252, 675
Bse1I ACTGG 2 cut(s) 525, 760
Bse3DI GCAATG 2 cut(s) 42, 1067
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 2 cut(s) 122, 787
BseDI CCNNGG 3 cut(s) 120, 301, 786
BseGI GGATG 1 cut(s) 363
BseLI CCNNNNNNNGG 3 cut(s) 306, 766, 1087
BseMI GCAATG 2 cut(s) 42, 1067
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 525, 760
BseRI GAGGAG 1 cut(s) 1002
BseXI GCAGC 2 cut(s) 61, 83
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 87, 182
BshFI GGCC 3 cut(s) 176, 899, 1080
BshNI GGYRCC 1 cut(s) 1141
BshTI ACCGGT 3 cut(s) 207, 252, 675
BsiHKAI GWGCWC 1 cut(s) 250
BsiSI CCGG 5 cut(s) 149, 208, 253, 500, 676
BslI CCNNNNNNNGG 3 cut(s) 306, 766, 1087
BsmAI GTCTC 3 cut(s) 138, 237, 772
BsmBI CGTCTC 1 cut(s) 772
BsnI GGCC 3 cut(s) 176, 899, 1080
Bso31I GGTCTC 1 cut(s) 138
Bsp119I TTCGAA 1 cut(s) 1132
Bsp1286I GDGCHC 1 cut(s) 250
Bsp13I TCCGGA 1 cut(s) 499
BspACI CCGC 2 cut(s) 39, 881
BspANI GGCC 3 cut(s) 176, 899, 1080
BspCNI CTCAG 1 cut(s) 130
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 2 cut(s) 87, 182
BspLI GGNNCC 2 cut(s) 472, 1143
BspMAI CTGCAG 1 cut(s) 73
BspQI GCTCTTC 2 cut(s) 105, 284
BspT104I TTCGAA 1 cut(s) 1132
BspT107I GGYRCC 1 cut(s) 1141
BspTNI GGTCTC 1 cut(s) 138
BsrDI GCAATG 2 cut(s) 42, 1067
BsrFI RCCGGY 3 cut(s) 207, 252, 675
BsrI ACTGG 2 cut(s) 525, 760
BssAI RCCGGY 3 cut(s) 207, 252, 675
BssECI CCNNGG 3 cut(s) 120, 301, 786
BssNI GRCGYC 2 cut(s) 630, 1142
BssT1I CCWWGG 1 cut(s) 301
Bst2UI CCWGG 2 cut(s) 122, 787
Bst4CI ACNGT 2 cut(s) 105, 418
Bst6I CTCTTC 2 cut(s) 105, 284
BstACI GRCGYC 2 cut(s) 630, 1142
BstBI TTCGAA 1 cut(s) 1132
BstC8I GCNNGC 4 cut(s) 117, 240, 369, 1028
BstDEI CTNAG 3 cut(s) 138, 363, 1110
BstEII GGTNACC 1 cut(s) 844
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstF5I GGATG 1 cut(s) 363
BstFNI CGCG 2 cut(s) 87, 182
BstH2I RGCGCY 1 cut(s) 1145
BstHHI GCGC 2 cut(s) 184, 1144
BstMAI GTCTC 3 cut(s) 138, 237, 772
BstMWI GCNNNNNNNGC 5 cut(s) 10, 44, 190, 479, 791
BstNI CCWGG 2 cut(s) 122, 787
BstNSI RCATGY 2 cut(s) 371, 1030
BstPI GGTNACC 1 cut(s) 844
BstSCI CCNGG 3 cut(s) 120, 147, 785
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 2 cut(s) 87, 182
BstV1I GCAGC 2 cut(s) 61, 83
BstV2I GAAGAC 1 cut(s) 228
BsuI GTATCC 3 cut(s) 517, 926, 1048
BsuRI GGCC 3 cut(s) 176, 899, 1080
BtsCI GGATG 1 cut(s) 363
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 432, 647
Cac8I GCNNGC 4 cut(s) 117, 240, 369, 1028
CaiI CAGNNNCTG 2 cut(s) 30, 766
CfoI GCGC 2 cut(s) 184, 1144
Cfr10I RCCGGY 3 cut(s) 207, 252, 675
CseI GACGC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 599
CspAI ACCGGT 3 cut(s) 207, 252, 675
CviAII CATG 6 cut(s) 226, 368, 923, 964, 1027, 1120
CviQI GTAC 1 cut(s) 599
DdeI CTNAG 3 cut(s) 138, 363, 1110
DinI GGCGCC 1 cut(s) 1143
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 2 cut(s) 105, 284
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 2 cut(s) 105, 284
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 301
Eco147I AGGCCT 1 cut(s) 1080
Eco24I GRGCYC 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 138
Eco53kI GAGCTC 1 cut(s) 248
Eco91I GGTNACC 1 cut(s) 844
EcoICRI GAGCTC 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoO65I GGTNACC 1 cut(s) 844
EcoRII CCWGG 2 cut(s) 120, 785
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 250
EgeI GGCGCC 1 cut(s) 1143
EheI GGCGCC 1 cut(s) 1143
ErhI CCWWGG 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 772
FaeI CATG 6 cut(s) 229, 371, 926, 967, 1030, 1123
FatI CATG 6 cut(s) 225, 367, 922, 963, 1026, 1119
FauI CCCGC 1 cut(s) 874
Fnu4HI GCNGC 3 cut(s) 39, 72, 75
FokI GGATG 1 cut(s) 370
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 3 cut(s) 39, 72, 75
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 183, 1143
GluI GCNGC 3 cut(s) 39, 72, 75
GsaI CCCAGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 264
HaeII RGCGCY 1 cut(s) 1145
HaeIII GGCC 3 cut(s) 176, 899, 1080
HapII CCGG 5 cut(s) 149, 208, 253, 500, 676
HgaI GACGC 1 cut(s) 66
HhaI GCGC 2 cut(s) 184, 1144
Hin1I GRCGYC 2 cut(s) 630, 1142
Hin1II CATG 6 cut(s) 229, 371, 926, 967, 1030, 1123
Hin6I GCGC 2 cut(s) 182, 1142
HinP1I GCGC 2 cut(s) 182, 1142
HindIII AAGCTT 1 cut(s) 128
HinfI GANTC 6 cut(s) 27, 451, 496, 703, 756, 967
HpaII CCGG 5 cut(s) 149, 208, 253, 500, 676
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 5 cut(s) 288, 450, 591, 960, 1047
Hpy188III TCNNGA 2 cut(s) 500, 890
HpyAV CCTTC 5 cut(s) 305, 308, 712, 1070, 1081
HpyCH4III ACNGT 2 cut(s) 105, 418
HpyCH4IV ACGT 1 cut(s) 630
HpyCH4V TGCA 8 cut(s) 71, 215, 394, 437, 817, 1026, 1030, 1177
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 44, 190, 479, 791
HpyF3I CTNAG 3 cut(s) 138, 363, 1110
HpySE526I ACGT 1 cut(s) 630
Hsp92I GRCGYC 2 cut(s) 630, 1142
Hsp92II CATG 6 cut(s) 229, 371, 926, 967, 1030, 1123
HspAI GCGC 2 cut(s) 182, 1142
KasI GGCGCC 1 cut(s) 1141
Kpn2I TCCGGA 1 cut(s) 499
LguI GCTCTTC 2 cut(s) 105, 284
LmnI GCTCC 2 cut(s) 245, 470
Lsp1109I GCAGC 2 cut(s) 61, 83
LweI GCATC 2 cut(s) 292, 804
MaeI CTAG 1 cut(s) 411
MaeII ACGT 1 cut(s) 630
MaeIII GTNAC 1 cut(s) 844
MboII GAAGA 4 cut(s) 122, 233, 301, 1146
MfeI CAATTG 1 cut(s) 1163
MhlI GDGCHC 1 cut(s) 250
MlsI TGGCCA 1 cut(s) 899
MluCI AATT 5 cut(s) 684, 853, 951, 1036, 1163
MluNI TGGCCA 1 cut(s) 899
Mly113I GGCGCC 1 cut(s) 1142
MlyI GAGTC 1 cut(s) 445
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 6 cut(s) 64, 321, 472, 835, 980, 1155
Mox20I TGGCCA 1 cut(s) 899
Mph1103I ATGCAT 1 cut(s) 396
MroI TCCGGA 1 cut(s) 499
MscI TGGCCA 1 cut(s) 899
MseI TTAA 5 cut(s) 126, 132, 171, 420, 579
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 899
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 5 cut(s) 149, 208, 253, 500, 676
MspR9I CCNGG 3 cut(s) 122, 149, 787
MunI CAATTG 1 cut(s) 1163
MvaI CCWGG 2 cut(s) 122, 787
MvnI CGCG 2 cut(s) 87, 182
MwoI GCNNNNNNNGC 5 cut(s) 10, 44, 190, 479, 791
NarI GGCGCC 1 cut(s) 1142
NciI CCSGG 1 cut(s) 149
NlaIII CATG 6 cut(s) 229, 371, 926, 967, 1030, 1123
NlaIV GGNNCC 2 cut(s) 472, 1143
NmeAIII GCCGAG 1 cut(s) 214
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 2 cut(s) 371, 1030
NspV TTCGAA 1 cut(s) 1132
PaeI GCATGC 2 cut(s) 371, 1030
PceI AGGCCT 1 cut(s) 1080
PciSI GCTCTTC 2 cut(s) 105, 284
PfeI GAWTC 5 cut(s) 27, 496, 703, 756, 967
PflMI CCANNNNNTGG 1 cut(s) 766
PinAI ACCGGT 3 cut(s) 207, 252, 675
PkrI GCNGC 3 cut(s) 40, 73, 76
PleI GAGTC 1 cut(s) 445
PluTI GGCGCC 1 cut(s) 1145
PpsI GAGTC 1 cut(s) 445
PsiI TTATAA 3 cut(s) 323, 335, 666
Psp124BI GAGCTC 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 120, 785
PspEI GGTNACC 1 cut(s) 844
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 2 cut(s) 120, 785
PspN4I GGNNCC 2 cut(s) 472, 1143
PstI CTGCAG 1 cut(s) 73
PstNI CAGNNNCTG 2 cut(s) 30, 766
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 600
RsaNI GTAC 1 cut(s) 599
RseI CAYNNNNRTG 1 cut(s) 399
SacI GAGCTC 1 cut(s) 250
SapI GCTCTTC 2 cut(s) 105, 284
SaqAI TTAA 5 cut(s) 126, 132, 171, 420, 579
SatI GCNGC 3 cut(s) 39, 72, 75
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 3 cut(s) 122, 149, 787
SduI GDGCHC 1 cut(s) 250
SfaNI GCATC 2 cut(s) 292, 804
SfcI CTRYAG 1 cut(s) 69
SfoI GGCGCC 1 cut(s) 1143
SfuI TTCGAA 1 cut(s) 1132
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 2 cut(s) 278, 440
SmoI CTYRAG 2 cut(s) 278, 440
SphI GCATGC 2 cut(s) 371, 1030
Sse9I AATT 5 cut(s) 684, 853, 951, 1036, 1163
SseBI AGGCCT 1 cut(s) 1080
SsiI CCGC 2 cut(s) 39, 881
SspDI GGCGCC 1 cut(s) 1141
SspI AATATT 2 cut(s) 541, 823
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 250
StuI AGGCCT 1 cut(s) 1080
StyD4I CCNGG 3 cut(s) 120, 147, 785
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 2 cut(s) 105, 418
TaiI ACGT 1 cut(s) 633
TaqI TCGA 2 cut(s) 56, 1132
TaqII GACCGA 1 cut(s) 523
TasI AATT 5 cut(s) 684, 853, 951, 1036, 1163
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 5 cut(s) 27, 496, 703, 756, 967
Tru1I TTAA 5 cut(s) 126, 132, 171, 420, 579
Tru9I TTAA 5 cut(s) 126, 132, 171, 420, 579
TscAI CASTG 2 cut(s) 439, 654
TseI GCWGC 2 cut(s) 71, 74
TspDTI ATGAA 3 cut(s) 152, 959, 1052
TspRI CASTG 2 cut(s) 439, 654
Van91I CCANNNNNTGG 1 cut(s) 766
XagI CCTNNNNNAGG 1 cut(s) 1085
XapI RAATTY 1 cut(s) 951
XceI RCATGY 2 cut(s) 371, 1030
XspI CTAG 1 cut(s) 411
ZraI GACGTC 1 cut(s) 631
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.