FvH4_6g30090

Protein PHLOEM PROTEIN 2-LIKE A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011580
Physical Location & Seq
Reverse (-)
23231260 .. 23232386
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30090.t1

Sequence Viewer

Length: 528 bp
ATGGCAGAGGCAAATTCTGTAAAAGTCGAATTTGTGGACTACAAAACAAAGAAACTATGGGTTGAAAAGATGTCCAACTACAAATGCGTGATGTTATTTCCAAGGCGGTTACATATCTCTAATCTGGTTCCTCCTTACTGGGCCTGGAATTGTTATAAAGAGACAAGTGACAACGTGGAGGTTGCCAAACTAAGGACTGTATGCTGGCTAGATGTGAGAGGCCACATAAAGATGTCTGAGCTCTCATGCGGCGTTGTTTATGAGATTGCGTACATAGTCAAATTAACAAACGGGGCATCTGGATGGGAGCTACCCATCACACTCAAACTCAGGTACCCCGATGGCAGAGAACAAATTCGCCAACAAAGTTTACTCGAGAAGCCGAGAGGAGAGTGGATGGAGCTCAGTGGTGGCACTTTTCTGACCAACCAAGAAGAAACCGGAGAGTTCTGGTTTGATCTCTGTGAACATGGTGGACACTGGAAAACTGGGCTTATCATCAAAGGTATTATTGCCAAGCCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

176

Amino Acids

20.32

Weight (kDa)

8.53

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 28 - 174 1.1e-30 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
Acc65I GGTACC 1 cut(s) 333
AccB1I GGYRCC 1 cut(s) 333
AciI CCGC 2 cut(s) 106, 249
AcsI RAATTY 3 cut(s) 13, 29, 354
AfaI GTAC 2 cut(s) 272, 335
AfiI CCNNNNNNNGG 1 cut(s) 192
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 3 cut(s) 241, 310, 403
AluI AGCT 3 cut(s) 241, 310, 403
Alw21I GWGCWC 2 cut(s) 243, 405
Alw26I GTCTC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 2 cut(s) 141, 220
ApoI RAATTY 3 cut(s) 13, 29, 354
Asp700I GAANNNNTTC 1 cut(s) 354
Asp718I GGTACC 1 cut(s) 333
AspS9I GGNCC 1 cut(s) 141
AvaI CYCGRG 1 cut(s) 374
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BanI GGYRCC 1 cut(s) 333
BanII GRGCYC 2 cut(s) 243, 405
Bbv12I GWGCWC 2 cut(s) 243, 405
BccI CCATC 4 cut(s) 297, 323, 335, 391
BciT130I CCWGG 1 cut(s) 145
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 1 cut(s) 209
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 145
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 2 cut(s) 129, 335
BmrFI CCNGG 1 cut(s) 145
BmrI ACTGGG 2 cut(s) 148, 498
BmsI GCATC 1 cut(s) 305
BmuI ACTGGG 2 cut(s) 148, 498
BsaJI CCNNGG 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 440
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 3 cut(s) 143, 485, 493
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 2 cut(s) 308, 402
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMII CTCAG 3 cut(s) 228, 343, 418
BseNI ACTGG 3 cut(s) 143, 485, 493
BseRI GAGGAG 1 cut(s) 402
BshFI GGCC 2 cut(s) 143, 222
BshNI GGYRCC 1 cut(s) 333
BsiHKAI GWGCWC 2 cut(s) 243, 405
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 1 cut(s) 441
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 143, 222
BsoBI CYCGRG 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 243, 405
Bsp143I GATC 1 cut(s) 457
BspACI CCGC 2 cut(s) 106, 249
BspANI GGCC 2 cut(s) 143, 222
BspCNI CTCAG 3 cut(s) 229, 342, 417
BspLI GGNNCC 2 cut(s) 129, 335
BspT107I GGYRCC 1 cut(s) 333
BsrI ACTGG 3 cut(s) 143, 485, 493
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 101
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 4 cut(s) 191, 237, 329, 404
BstF5I GGATG 2 cut(s) 308, 402
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 1 cut(s) 457
BstNI CCWGG 1 cut(s) 145
BstSCI CCNGG 1 cut(s) 143
BsuRI GGCC 2 cut(s) 143, 222
BtsCI GGATG 2 cut(s) 308, 402
BtsIMutI CAGTG 2 cut(s) 412, 478
Cac8I GCNNGC 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 271, 334
CviAII CATG 2 cut(s) 246, 470
CviJI RGCY 9 cut(s) 143, 208, 222, 241, 310, 382, 403, 493, 520
CviKI_1 RGCY 9 cut(s) 143, 208, 222, 241, 310, 382, 403, 493, 520
CviQI GTAC 2 cut(s) 271, 334
DdeI CTNAG 4 cut(s) 191, 237, 329, 404
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
Ecl136II GAGCTC 2 cut(s) 241, 403
Eco130I CCWWGG 1 cut(s) 101
Eco24I GRGCYC 2 cut(s) 243, 405
Eco53kI GAGCTC 2 cut(s) 241, 403
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 2 cut(s) 241, 403
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 101
EcoT38I GRGCYC 2 cut(s) 243, 405
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 2 cut(s) 249, 473
FaiI YATR 9 cut(s) 58, 114, 156, 202, 227, 247, 261, 275, 471
FatI CATG 2 cut(s) 245, 469
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 2 cut(s) 315, 409
FriOI GRGCYC 2 cut(s) 243, 405
Fsp4HI GCNGC 1 cut(s) 250
FspBI CTAG 1 cut(s) 209
GluI GCNGC 1 cut(s) 250
HaeIII GGCC 2 cut(s) 143, 222
HapII CCGG 1 cut(s) 441
Hin1II CATG 2 cut(s) 249, 473
HpaII CCGG 1 cut(s) 441
Hpy166II GTNNAC 4 cut(s) 37, 371, 467, 476
Hpy188I TCNGA 2 cut(s) 238, 423
Hpy188III TCNNGA 2 cut(s) 300, 376
Hpy8I GTNNAC 4 cut(s) 37, 371, 467, 476
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 174
HpyF3I CTNAG 4 cut(s) 191, 237, 329, 404
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 2 cut(s) 249, 473
KpnI GGTACC 1 cut(s) 337
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 2 cut(s) 307, 400
LweI GCATC 1 cut(s) 305
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 108, 167
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 1 cut(s) 446
MhlI GDGCHC 2 cut(s) 243, 405
MluCI AATT 5 cut(s) 13, 29, 148, 281, 354
MmeI TCCRAC 1 cut(s) 99
MnlI CCTC 4 cut(s) 141, 172, 212, 380
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 2 cut(s) 230, 301
MspI CCGG 1 cut(s) 441
MspR9I CCNGG 1 cut(s) 145
MvaI CCWGG 1 cut(s) 145
NdeII GATC 1 cut(s) 457
NlaIII CATG 2 cut(s) 249, 473
NlaIV GGNNCC 2 cut(s) 129, 335
NmeAIII GCCGAG 1 cut(s) 408
NmuCI GTSAC 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 374
PdmI GAANNNNTTC 1 cut(s) 354
PkrI GCNGC 1 cut(s) 251
PsiI TTATAA 1 cut(s) 156
Psp124BI GAGCTC 2 cut(s) 243, 405
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 129, 335
PspPI GGNCC 1 cut(s) 141
RsaI GTAC 2 cut(s) 272, 335
RsaNI GTAC 2 cut(s) 271, 334
RseI CAYNNNNRTG 2 cut(s) 230, 301
SacI GAGCTC 2 cut(s) 243, 405
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 145
SduI GDGCHC 2 cut(s) 243, 405
SetI ASST 7 cut(s) 177, 183, 243, 312, 335, 405, 508
SfaNI GCATC 1 cut(s) 305
Sfr274I CTCGAG 1 cut(s) 374
SlaI CTCGAG 1 cut(s) 374
SmiMI CAYNNNNRTG 2 cut(s) 230, 301
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 5 cut(s) 13, 29, 148, 281, 354
SsiI CCGC 2 cut(s) 106, 249
SspMI CTAG 1 cut(s) 209
SstI GAGCTC 2 cut(s) 243, 405
StyD4I CCNGG 1 cut(s) 143
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 177
TaqI TCGA 2 cut(s) 27, 375
TasI AATT 5 cut(s) 13, 29, 148, 281, 354
TauI GCSGC 1 cut(s) 252
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TscAI CASTG 2 cut(s) 412, 485
TseFI GTSAC 1 cut(s) 167
Tsp45I GTSAC 1 cut(s) 167
TspRI CASTG 2 cut(s) 412, 485
XapI RAATTY 3 cut(s) 13, 29, 354
XhoI CTCGAG 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.