Rw2G030990

Protein PHLOEM PROTEIN 2-LIKE A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011580
Physical Location & Seq
Reverse (-)
50912392 .. 50926297
13906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030990.1

Sequence Viewer

Length: 531 bp
ATGGCAGAGCCAACGAAGGGAGAATTCCGGGGACTTAAAAGAATGAAAATATGGAAGGAGAAGAAAACTGGCTACAAATGCATGATGTTGTTCCCAACGAGTCTCAGTGTTGTTAATGGTGGTCCTCCATATTGGGTTTGGGATTGTTATGAGGAAACAAGTGACGAGAACACTGTGGTAGCCAAACTAAATGATGTATGTTGGCTGGAGGTAACAGGACACTTCAACATGTCAGAGCTCTCAGCAGGAATTGATTATGAGATTGCGTACAGAGTCAAATTAACGACTGCAGCAACTGGATGGGAATTCCCTGTTACTCTCAATGTCAGACTCCCGAATGATTCAAAAGTCCGAAAGCGCCAATACAGTCTATTCGAGAAGCCCAGAGAAGAGTGGATAGACCTGGTCGGTGGCCATTTTAAGGTCAAGAAAGGAGAAACTGGAGAAGCGCACTTTGATCTTGCTGGAAGTGGCGGACATTGGAAGAGTGGGCTTATCATCCAAGGTATCATTATCAAGCCGAAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

176

Amino Acids

20.02

Weight (kDa)

8.67

Isoelectric Point (pI)

17.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 26 - 174 1.6e-29 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 474
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 2 cut(s) 23, 305
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 2 cut(s) 17, 421
AflIII ACRYGT 1 cut(s) 228
AgsI TTSAA 2 cut(s) 226, 345
AjnI CCWGG 1 cut(s) 402
AluBI AGCT 1 cut(s) 238
AluI AGCT 1 cut(s) 238
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 107
AlwNI CAGNNNCTG 1 cut(s) 296
AoxI GGCC 1 cut(s) 412
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 2 cut(s) 23, 305
AspLEI GCGC 2 cut(s) 360, 451
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 1 cut(s) 29
AvaII GGWCC 1 cut(s) 122
BalI TGGCCA 1 cut(s) 414
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 302
BccI CCATC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 404
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 107
BfmI CTRYAG 1 cut(s) 288
BfoI RGCGCY 1 cut(s) 361
BisI GCNGC 1 cut(s) 291
BlsI GCNGC 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 29, 404
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 29, 404
BpmI CTGGAG 2 cut(s) 227, 462
BpuMI CCSGG 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 28, 502
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 421
Bse1I ACTGG 3 cut(s) 73, 301, 445
BseBI CCWGG 1 cut(s) 404
BseDI CCNNGG 2 cut(s) 28, 502
BseGI GGATG 2 cut(s) 305, 498
BseLI CCNNNNNNNGG 2 cut(s) 17, 421
BseMII CTCAG 2 cut(s) 118, 255
BseNI ACTGG 3 cut(s) 73, 301, 445
BseXI GCAGC 1 cut(s) 302
BshFI GGCC 1 cut(s) 414
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 28
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 17, 421
BsmAI GTCTC 1 cut(s) 107
BsmFI GGGAC 1 cut(s) 45
BsnI GGCC 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 1 cut(s) 457
BspACI CCGC 1 cut(s) 474
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 2 cut(s) 117, 254
BspMAI CTGCAG 1 cut(s) 292
BsrI ACTGG 3 cut(s) 73, 301, 445
BssECI CCNNGG 2 cut(s) 28, 502
BssMI GATC 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 502
Bst2UI CCWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 175, 368
Bst6I CTCTTC 2 cut(s) 384, 479
BstDEI CTNAG 2 cut(s) 104, 241
BstF5I GGATG 2 cut(s) 305, 498
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 2 cut(s) 360, 451
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 1 cut(s) 107
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstNI CCWGG 1 cut(s) 404
BstNSI RCATGY 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 27, 402
BstSFI CTRYAG 1 cut(s) 288
BstV1I GCAGC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 414
BtsCI GGATG 2 cut(s) 305, 498
BtsIMutI CAGTG 2 cut(s) 112, 171
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 2 cut(s) 360, 451
Cfr13I GGNCC 1 cut(s) 122
CsiI ACCWGGT 1 cut(s) 402
Csp6I GTAC 1 cut(s) 268
CviAII CATG 2 cut(s) 82, 229
CviJI RGCY 9 cut(s) 10, 72, 182, 205, 238, 382, 414, 493, 520
CviKI_1 RGCY 9 cut(s) 10, 72, 182, 205, 238, 382, 414, 493, 520
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 104, 241
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 2 cut(s) 384, 479
EarI CTCTTC 2 cut(s) 384, 479
EciI GGCGGA 1 cut(s) 489
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 122
Eco53kI GAGCTC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 238
EcoRI GAATTC 2 cut(s) 23, 305
EcoRII CCWGG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 502
EcoT22I ATGCAT 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 502
FaeI CATG 2 cut(s) 85, 232
FaiI YATR 7 cut(s) 52, 83, 130, 150, 199, 230, 258
FaqI GGGAC 1 cut(s) 45
FatI CATG 2 cut(s) 81, 228
Fnu4HI GCNGC 1 cut(s) 291
FokI GGATG 2 cut(s) 312, 485
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 1 cut(s) 291
GlaI GCGC 2 cut(s) 359, 450
GluI GCNGC 1 cut(s) 291
GsuI CTGGAG 2 cut(s) 227, 462
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 1 cut(s) 414
HapII CCGG 1 cut(s) 28
HhaI GCGC 2 cut(s) 360, 451
Hin1II CATG 2 cut(s) 85, 232
Hin6I GCGC 2 cut(s) 358, 449
HinP1I GCGC 2 cut(s) 358, 449
HinfI GANTC 4 cut(s) 100, 273, 330, 341
HpaII CCGG 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 235, 329, 353
Hpy188III TCNNGA 3 cut(s) 334, 376, 427
HpyAV CCTTC 2 cut(s) 10, 49
HpyCH4III ACNGT 2 cut(s) 175, 368
HpyCH4V TGCA 2 cut(s) 81, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 2 cut(s) 104, 241
Hsp92II CATG 2 cut(s) 85, 232
HspAI GCGC 2 cut(s) 358, 449
Kzo9I GATC 1 cut(s) 457
Lsp1109I GCAGC 1 cut(s) 302
MabI ACCWGGT 1 cut(s) 402
MaeIII GTNAC 3 cut(s) 161, 211, 313
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 3 cut(s) 73, 401, 496
MhlI GDGCHC 1 cut(s) 240
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 4 cut(s) 23, 249, 278, 305
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 3 cut(s) 109, 282, 324
MnlI CCTC 3 cut(s) 135, 145, 202
Mox20I TGGCCA 1 cut(s) 414
Mph1103I ATGCAT 1 cut(s) 83
MscI TGGCCA 1 cut(s) 414
MseI TTAA 5 cut(s) 36, 114, 281, 420, 529
Msp20I TGGCCA 1 cut(s) 414
MspI CCGG 1 cut(s) 28
MspR9I CCNGG 2 cut(s) 29, 404
MvaI CCWGG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 78
NciI CCSGG 1 cut(s) 29
NdeII GATC 1 cut(s) 457
NlaIII CATG 2 cut(s) 85, 232
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 83
NspI RCATGY 1 cut(s) 232
PciI ACATGT 1 cut(s) 228
PfeI GAWTC 1 cut(s) 341
PflFI GACNNNGTC 1 cut(s) 404
PkrI GCNGC 1 cut(s) 292
PleI GAGTC 3 cut(s) 108, 281, 324
PpsI GAGTC 3 cut(s) 108, 281, 324
PscI ACATGT 1 cut(s) 228
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 402
PspGI CCWGG 1 cut(s) 402
PspPI GGNCC 1 cut(s) 122
PstI CTGCAG 1 cut(s) 292
PstNI CAGNNNCTG 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 404
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 5 cut(s) 36, 114, 281, 420, 529
SatI GCNGC 1 cut(s) 291
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 1 cut(s) 122
SchI GAGTC 3 cut(s) 109, 282, 324
ScrFI CCNGG 2 cut(s) 29, 404
SduI GDGCHC 1 cut(s) 240
SetI ASST 5 cut(s) 213, 240, 405, 426, 508
SexAI ACCWGGT 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 288
SinI GGWCC 1 cut(s) 122
Sse9I AATT 4 cut(s) 23, 249, 278, 305
SsiI CCGC 1 cut(s) 474
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 2 cut(s) 27, 402
StyI CCWWGG 1 cut(s) 502
TaaI ACNGT 2 cut(s) 175, 368
TaqI TCGA 1 cut(s) 375
TasI AATT 4 cut(s) 23, 249, 278, 305
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 5 cut(s) 36, 114, 281, 420, 529
Tru9I TTAA 5 cut(s) 36, 114, 281, 420, 529
TscAI CASTG 2 cut(s) 112, 178
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 1 cut(s) 59
TspRI CASTG 2 cut(s) 112, 178
Tth111I GACNNNGTC 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 2 cut(s) 23, 305
XceI RCATGY 1 cut(s) 232
XcmI CCANNNNNNNNNTGG 1 cut(s) 135
Zsp2I ATGCAT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.