Prupe.3G082500_v2.0.a1

Protein PHLOEM PROTEIN 2-LIKE A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0011580
Physical Location & Seq
Reverse (-)
6106217 .. 6107270
1054 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G082500.1

Sequence Viewer

Length: 564 bp
ATGGCGGATTCTTCGAAAGGCGAATTCCAGGAGTTCAAGAAAAGGAAAGTATGGTTTGAGAAGAAATCTAGATGCATGGAGTTGTACCCAAGATGGTTAGATGTCACTTGGGGTTTTCCTCCAAACTGGGTTTGGAATTGTTACAAAGAAACAAGTGAGGACAACGTCGAGGTAGTCAAGCTATTGGATGTATGCTTTCTGAATGTGAGAGGACAATTCGAGATGTCAGAGCTCTCAGCAGGAGTTGTTTATGAGATTGCATACAAAGTCAAACTAACAACCGGAGCATATGGATGGGAATTCCCGGTTACGGTTAAGATCGGATTCCCAGATGGAAGGGAGCAAAAGCGGAAATATAGTCTGTTTCATAAGCCGAGAGGGGAGTGGATAGAGCTTAGTGGTGGTAGTTTTGAGGTTAACGGAGAAGAAACTGGAAAAGTGTGGTTTGATCTTTGTCAACATGGGGGACACTGGAAGAGAGGGCTTATCATCCAAGGTATCATCATCAAGCCGGCTGAAAAGAATCAATCCACAGATACAAGTACAGGGTTTTGTTGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

188

Amino Acids

21.76

Weight (kDa)

7.57

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 349
AcsI RAATTY 2 cut(s) 23, 299
AfaI GTAC 2 cut(s) 86, 544
AfiI CCNNNNNNNGG 1 cut(s) 310
AgsI TTSAA 1 cut(s) 37
AjnI CCWGG 1 cut(s) 27
AluBI AGCT 3 cut(s) 181, 232, 394
AluI AGCT 3 cut(s) 181, 232, 394
Alw21I GWGCWC 1 cut(s) 234
ApoI RAATTY 2 cut(s) 23, 299
AsuC2I CCSGG 1 cut(s) 305
AsuII TTCGAA 1 cut(s) 14
BanII GRGCYC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 234
BccI CCATC 3 cut(s) 87, 288, 326
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 1 cut(s) 305
BfaI CTAG 1 cut(s) 69
Bme1390I CCNGG 2 cut(s) 29, 305
BmrFI CCNGG 2 cut(s) 29, 305
BmrI ACTGGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 136
Bpu14I TTCGAA 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 305
BsaJI CCNNGG 1 cut(s) 493
BsaWI WCCGGW 1 cut(s) 281
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 3 cut(s) 131, 436, 476
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 3 cut(s) 193, 299, 489
BseLI CCNNNNNNNGG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 3 cut(s) 131, 436, 476
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 3 cut(s) 282, 305, 512
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 1 cut(s) 310
BsmFI GGGAC 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 2 cut(s) 318, 448
BspACI CCGC 2 cut(s) 5, 349
BspCNI CTCAG 1 cut(s) 248
BspT104I TTCGAA 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 3 cut(s) 131, 436, 476
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 2 cut(s) 318, 448
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 313
Bst6I CTCTTC 1 cut(s) 470
BstBI TTCGAA 1 cut(s) 14
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 2 cut(s) 235, 395
BstF5I GGATG 3 cut(s) 193, 299, 489
BstKTI GATC 2 cut(s) 321, 451
BstMBI GATC 2 cut(s) 318, 448
BstNI CCWGG 1 cut(s) 29
BstSCI CCNGG 2 cut(s) 27, 303
BtsCI GGATG 3 cut(s) 193, 299, 489
BtsIMutI CAGTG 1 cut(s) 469
Cac8I GCNNGC 1 cut(s) 513
Cfr10I RCCGGY 1 cut(s) 511
Csp6I GTAC 2 cut(s) 85, 543
CviAII CATG 2 cut(s) 76, 461
CviJI RGCY 7 cut(s) 181, 232, 373, 394, 484, 511, 515
CviKI_1 RGCY 7 cut(s) 181, 232, 373, 394, 484, 511, 515
CviQI GTAC 2 cut(s) 85, 543
DdeI CTNAG 2 cut(s) 235, 395
DpnI GATC 2 cut(s) 320, 450
DpnII GATC 2 cut(s) 318, 448
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoRI GAATTC 2 cut(s) 23, 299
EcoRII CCWGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 77
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 2 cut(s) 79, 464
FaqI GGGAC 1 cut(s) 480
FatI CATG 2 cut(s) 75, 460
FauNDI CATATG 1 cut(s) 289
FokI GGATG 3 cut(s) 200, 306, 476
FriOI GRGCYC 1 cut(s) 234
FspBI CTAG 1 cut(s) 69
HapII CCGG 3 cut(s) 282, 305, 512
Hin1II CATG 2 cut(s) 79, 464
HincII GTYRAC 2 cut(s) 418, 458
HindII GTYRAC 2 cut(s) 418, 458
HinfI GANTC 3 cut(s) 8, 324, 523
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 3 cut(s) 282, 305, 512
Hpy166II GTNNAC 2 cut(s) 418, 458
Hpy188I TCNGA 3 cut(s) 201, 229, 323
Hpy188III TCNNGA 3 cut(s) 37, 69, 220
Hpy8I GTNNAC 2 cut(s) 418, 458
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 1 cut(s) 330
HpyCH4III ACNGT 1 cut(s) 313
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 2 cut(s) 75, 260
HpyF3I CTNAG 2 cut(s) 235, 395
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 2 cut(s) 79, 464
KroI GCCGGC 1 cut(s) 511
KroNI GCCGGC 1 cut(s) 513
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 2 cut(s) 318, 448
LmnI GCTCC 2 cut(s) 284, 340
LweI GCATC 1 cut(s) 62
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 3 cut(s) 103, 140, 307
MalI GATC 2 cut(s) 320, 450
MboI GATC 2 cut(s) 318, 448
MboII GAAGA 4 cut(s) 3, 73, 437, 487
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 4 cut(s) 23, 136, 215, 299
MnlI CCTC 7 cut(s) 129, 151, 163, 203, 371, 406, 473
Mph1103I ATGCAT 1 cut(s) 77
MroNI GCCGGC 1 cut(s) 511
MseI TTAA 2 cut(s) 315, 417
MslI CAYNNNNRTG 1 cut(s) 292
MspI CCGG 3 cut(s) 282, 305, 512
MspR9I CCNGG 2 cut(s) 29, 305
MvaI CCWGG 1 cut(s) 29
NaeI GCCGGC 1 cut(s) 513
NciI CCSGG 1 cut(s) 305
NdeI CATATG 1 cut(s) 289
NdeII GATC 2 cut(s) 318, 448
NgoMIV GCCGGC 1 cut(s) 511
NlaIII CATG 2 cut(s) 79, 464
NmeAIII GCCGAG 1 cut(s) 399
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 77
NspV TTCGAA 1 cut(s) 14
PdiI GCCGGC 1 cut(s) 513
PfeI GAWTC 3 cut(s) 8, 324, 523
PflFI GACNNNGTC 1 cut(s) 164
PfoI TCCNGGA 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 2 cut(s) 86, 544
RsaNI GTAC 2 cut(s) 85, 543
RseI CAYNNNNRTG 1 cut(s) 292
SacI GAGCTC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 315, 417
Sau3AI GATC 2 cut(s) 318, 448
ScrFI CCNGG 2 cut(s) 29, 305
SduI GDGCHC 1 cut(s) 234
SetI ASST 7 cut(s) 168, 174, 183, 234, 396, 417, 499
SfaNI GCATC 1 cut(s) 62
SfuI TTCGAA 1 cut(s) 14
SmiMI CAYNNNNRTG 1 cut(s) 292
Sse9I AATT 4 cut(s) 23, 136, 215, 299
SsiI CCGC 2 cut(s) 5, 349
SspMI CTAG 1 cut(s) 69
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 27, 303
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 1 cut(s) 313
TaiI ACGT 1 cut(s) 168
TaqI TCGA 3 cut(s) 14, 168, 219
TasI AATT 4 cut(s) 23, 136, 215, 299
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 3 cut(s) 8, 324, 523
Tru1I TTAA 2 cut(s) 315, 417
Tru9I TTAA 2 cut(s) 315, 417
TscAI CASTG 1 cut(s) 476
TseFI GTSAC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 1 cut(s) 356
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 1 cut(s) 476
Tth111I GACNNNGTC 1 cut(s) 164
XapI RAATTY 2 cut(s) 23, 299
XbaI TCTAGA 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 129
XspI CTAG 1 cut(s) 69
Zsp2I ATGCAT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.