MD17G1184600.v1.1

Protein PHLOEM PROTEIN 2-LIKE A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0011580
Physical Location & Seq
Forward (+)
21785739 .. 21787514
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1184600.v1.1.491

Sequence Viewer

Length: 534 bp
ATGAAAGATTCAACAAATGGAGAATTCCGGGACTTCAAGAAAATGAAAATATGGGTTAAGAAGAACTCTGGCTACAAATGCATGATGTTATACGCAAGGTCCTTAGCAATCACTTGGGGTGGTCCTCCGTACTGGGTTTGGAATTGTTATAAAGAGACAGGGGATGACAACATTGAGGTAGCCAAACTAACGGGTGTACGCGATCTAGATGTCCAAGGACGGTTCAAGATGTCGGAGCTCTCACCAGGAGTTGTTTATGAGATTGCCTACATTGTTAAATTAACAAACGGAGCATCTGGATGGGAACTCCCTGTTACACTCAAAATCACTCTCCCGGGGCAGGGCGGAAGAGAAAAGAAGCGCCAATACAGTCTACTCGAGAAGCCGAGAGGAGTGTGGATGGAGCTTGTTGGTGGCAGTTTTCAGTCAATGGCAAGAGAAACTGGAGAAGTGATCTTTGATTTTTATAATCATGACACGCCCTCGAAAAGTGGGCTTATCATCAAAGGTATCATTATCAGGCCAAAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

178

Amino Acids

20.12

Weight (kDa)

9.43

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 26 - 175 1.2e-29 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 150, 468
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 201
AciI CCGC 1 cut(s) 345
AcsI RAATTY 1 cut(s) 23
AfaI GTAC 2 cut(s) 131, 198
AfiI CCNNNNNNNGG 2 cut(s) 340, 341
AgsI TTSAA 3 cut(s) 12, 37, 226
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 2 cut(s) 238, 406
AluI AGCT 2 cut(s) 238, 406
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 1 cut(s) 149
Ama87I CYCGRG 2 cut(s) 334, 377
AoxI GGCC 1 cut(s) 521
ApoI RAATTY 1 cut(s) 23
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 2 cut(s) 99, 122
AsuC2I CCSGG 3 cut(s) 29, 335, 336
AsuHPI GGTGA 1 cut(s) 234
AvaI CYCGRG 2 cut(s) 334, 377
AvaII GGWCC 2 cut(s) 99, 122
BanII GRGCYC 1 cut(s) 240
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
Bbv12I GWGCWC 1 cut(s) 240
BccI CCATC 2 cut(s) 294, 394
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 3 cut(s) 29, 335, 336
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 1 cut(s) 206
BfoI RGCGCY 1 cut(s) 364
Bme1390I CCNGG 4 cut(s) 29, 246, 335, 336
Bme18I GGWCC 2 cut(s) 99, 122
BmeT110I CYCGRG 2 cut(s) 334, 377
BmgT120I GGNCC 2 cut(s) 99, 122
BmrFI CCNGG 4 cut(s) 29, 246, 335, 336
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 302
BmuI ACTGGG 1 cut(s) 142
BpmI CTGGAG 1 cut(s) 465
Bpu10I CCTNAGC 1 cut(s) 103
BpuMI CCSGG 3 cut(s) 29, 335, 336
BsaJI CCNNGG 3 cut(s) 214, 334, 335
Bsc4I CCNNNNNNNGG 2 cut(s) 340, 341
Bse1I ACTGG 2 cut(s) 137, 448
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 214, 334, 335
BseGI GGATG 3 cut(s) 169, 305, 405
BseLI CCNNNNNNNGG 2 cut(s) 340, 341
BseNI ACTGG 2 cut(s) 137, 448
BseRI GAGGAG 1 cut(s) 405
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 523
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 2 cut(s) 334, 377
BsiSI CCGG 2 cut(s) 28, 335
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 2 cut(s) 340, 341
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 1 cut(s) 523
BsoBI CYCGRG 2 cut(s) 334, 377
Bsp1286I GDGCHC 1 cut(s) 240
Bsp143I GATC 2 cut(s) 202, 453
BspACI CCGC 1 cut(s) 345
BspANI GGCC 1 cut(s) 523
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 472
BsrI ACTGG 2 cut(s) 137, 448
BssECI CCNNGG 3 cut(s) 214, 334, 335
BssMI GATC 2 cut(s) 202, 453
BssT1I CCWWGG 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 2 cut(s) 222, 371
Bst6I CTCTTC 1 cut(s) 343
BstDEI CTNAG 1 cut(s) 103
BstF5I GGATG 3 cut(s) 169, 305, 405
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 2 cut(s) 205, 456
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 2 cut(s) 202, 453
BstMWI GCNNNNNNNGC 1 cut(s) 78
BstNI CCWGG 1 cut(s) 246
BstSCI CCNGG 4 cut(s) 27, 244, 333, 334
BstUI CGCG 1 cut(s) 201
BsuRI GGCC 1 cut(s) 523
BtsCI GGATG 3 cut(s) 169, 305, 405
CciI TCATGA 1 cut(s) 472
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 2 cut(s) 99, 122
Cfr9I CCCGGG 1 cut(s) 334
Csp6I GTAC 2 cut(s) 130, 197
CviAII CATG 2 cut(s) 82, 473
CviJI RGCY 7 cut(s) 72, 182, 238, 385, 406, 496, 523
CviKI_1 RGCY 7 cut(s) 72, 182, 238, 385, 406, 496, 523
CviQI GTAC 2 cut(s) 130, 197
DdeI CTNAG 1 cut(s) 103
DpnI GATC 2 cut(s) 204, 455
DpnII GATC 2 cut(s) 202, 453
Eam1104I CTCTTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 343
EciI GGCGGA 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 2 cut(s) 99, 122
Eco53kI GAGCTC 1 cut(s) 238
Eco88I CYCGRG 2 cut(s) 334, 377
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 1 cut(s) 99
EcoRI GAATTC 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 83
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 2 cut(s) 85, 476
FaiI YATR 7 cut(s) 52, 83, 91, 150, 258, 468, 474
FaqI GGGAC 1 cut(s) 44
FatI CATG 2 cut(s) 81, 472
FblI GTMKAC 1 cut(s) 373
FokI GGATG 3 cut(s) 176, 312, 412
FriOI GRGCYC 1 cut(s) 240
FspBI CTAG 1 cut(s) 206
GlaI GCGC 1 cut(s) 362
GsuI CTGGAG 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 1 cut(s) 523
HapII CCGG 2 cut(s) 28, 335
HhaI GCGC 1 cut(s) 363
Hin1II CATG 2 cut(s) 85, 476
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HinfI GANTC 1 cut(s) 8
HpaII CCGG 2 cut(s) 28, 335
HphI GGTGA 1 cut(s) 234
Hpy166II GTNNAC 2 cut(s) 197, 374
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 6 cut(s) 37, 206, 226, 297, 379, 473
Hpy8I GTNNAC 2 cut(s) 197, 374
HpyCH4III ACNGT 2 cut(s) 222, 371
HpyCH4V TGCA 1 cut(s) 81
HpyF10VI GCNNNNNNNGC 1 cut(s) 78
HpyF3I CTNAG 1 cut(s) 103
Hsp92II CATG 2 cut(s) 85, 476
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 2 cut(s) 202, 453
LmnI GCTCC 3 cut(s) 235, 290, 403
LweI GCATC 1 cut(s) 302
MaeI CTAG 1 cut(s) 206
MaeIII GTNAC 1 cut(s) 313
MalI GATC 2 cut(s) 204, 455
MboI GATC 2 cut(s) 202, 453
MboII GAAGA 2 cut(s) 73, 360
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 4 cut(s) 23, 142, 278, 529
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 4 cut(s) 135, 169, 383, 493
Mph1103I ATGCAT 1 cut(s) 83
MseI TTAA 3 cut(s) 57, 276, 281
MslI CAYNNNNRTG 1 cut(s) 298
MspI CCGG 2 cut(s) 28, 335
MspR9I CCNGG 4 cut(s) 29, 246, 335, 336
MvaI CCWGG 1 cut(s) 246
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 1 cut(s) 78
NciI CCSGG 3 cut(s) 29, 335, 336
NdeII GATC 2 cut(s) 202, 453
NlaIII CATG 2 cut(s) 85, 476
NmeAIII GCCGAG 1 cut(s) 411
NsiI ATGCAT 1 cut(s) 83
PaeR7I CTCGAG 1 cut(s) 377
PagI TCATGA 1 cut(s) 472
PfeI GAWTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 27
PpuMI RGGWCCY 1 cut(s) 99
PsiI TTATAA 2 cut(s) 150, 468
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspPI GGNCC 2 cut(s) 99, 122
PspPPI RGGWCCY 1 cut(s) 99
PsrI GAACNNNNNNTAC 2 cut(s) 56, 88
RsaI GTAC 2 cut(s) 131, 198
RsaNI GTAC 2 cut(s) 130, 197
RseI CAYNNNNRTG 1 cut(s) 298
SacI GAGCTC 1 cut(s) 240
SaqAI TTAA 3 cut(s) 57, 276, 281
Sau3AI GATC 2 cut(s) 202, 453
Sau96I GGNCC 2 cut(s) 99, 122
ScrFI CCNGG 4 cut(s) 29, 246, 335, 336
SduI GDGCHC 1 cut(s) 240
SetI ASST 5 cut(s) 101, 180, 240, 408, 511
SfaNI GCATC 1 cut(s) 302
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 2 cut(s) 99, 122
SlaI CTCGAG 1 cut(s) 377
SmaI CCCGGG 1 cut(s) 336
SmiMI CAYNNNNRTG 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 4 cut(s) 23, 142, 278, 529
SsiI CCGC 1 cut(s) 345
SspMI CTAG 1 cut(s) 206
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 4 cut(s) 27, 244, 333, 334
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 2 cut(s) 222, 371
TaqI TCGA 2 cut(s) 378, 485
TasI AATT 4 cut(s) 23, 142, 278, 529
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 57, 276, 281
Tru9I TTAA 3 cut(s) 57, 276, 281
TspDTI ATGAA 2 cut(s) 17, 59
TspGWI ACGGA 2 cut(s) 117, 303
TspMI CCCGGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 99, 122
XapI RAATTY 1 cut(s) 23
XbaI TCTAGA 1 cut(s) 205
XhoI CTCGAG 1 cut(s) 377
XmaI CCCGGG 1 cut(s) 334
XmiI GTMKAC 1 cut(s) 373
XspI CTAG 1 cut(s) 206
Zsp2I ATGCAT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.