Rroxscaffold_2G00108400

Protein PHLOEM PROTEIN 2-LIKE A1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011580
Physical Location & Seq
Forward (+)
32388633 .. 32389782
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108400.1

Sequence Viewer

Length: 534 bp
ATGGAGGAGGCAAATTCAGCAAAAGTCGAATTCGTGGACTACAGAACAAAGAAACTATGGATTGAAAAGATGTCCAGCTACAAATGCGTAATGTTATGTCCAAGGCGGTTACAAATCGCTCATGGTCCTCCATACTGGACCTGGAATTGTTATAAAGAGACGTGTAAAATTGACAATGTGGAGGTGGCGAAACTATCGCGTGTATGCTGGCTAAATCTGAGAGGACACATAAAGATGTCTGAGCTCTCATCCGGCGTAGAGTATGAGATTGCATACATAGTCAAACTAACAAATGGAGCATCTGGATGGGAACTCCCTATCACACTCAAACTAAGGCACCCAGATGGCAGAGAACAAACGCGCCGACAAAGTATACTCGAGAAGCCGAGAGGAGAGTGGATGGAGCTCAGTGGTGGTACCTTTCGGACCAACCAAGAAGAAACTGGAGAATTCTGGTTTGATCTTTGTGAACATGGTGGACACTGGAAAACTGGGCTCATCGTCAAGGGTATTATTGCCAAGCCGAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

177

Amino Acids

20.62

Weight (kDa)

8.86

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 28 - 175 1.4e-29 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 153
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 2 cut(s) 336, 416
AccI GTMKAC 1 cut(s) 373
AccII CGCG 2 cut(s) 199, 361
AciI CCGC 1 cut(s) 106
AcsI RAATTY 3 cut(s) 13, 29, 449
AfaI GTAC 1 cut(s) 418
AflIII ACRYGT 1 cut(s) 161
AgsI TTSAA 1 cut(s) 65
AjiI CACGTC 1 cut(s) 162
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 3 cut(s) 78, 244, 406
AluI AGCT 3 cut(s) 78, 244, 406
Alw21I GWGCWC 2 cut(s) 246, 408
Alw26I GTCTC 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 377
ApoI RAATTY 3 cut(s) 13, 29, 449
Asp718I GGTACC 1 cut(s) 416
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 3 cut(s) 125, 138, 426
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 3 cut(s) 125, 138, 426
BanI GGYRCC 2 cut(s) 336, 416
BanII GRGCYC 3 cut(s) 246, 408, 498
Bbv12I GWGCWC 2 cut(s) 246, 408
BccI CCATC 3 cut(s) 300, 338, 394
BciT130I CCWGG 1 cut(s) 142
BcoDI GTCTC 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 3 cut(s) 125, 138, 426
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 162
BmgT120I GGNCC 3 cut(s) 125, 138, 426
BmiI GGNNCC 2 cut(s) 338, 418
BmrFI CCNGG 1 cut(s) 142
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 308
BmuI ACTGGG 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 101
Bse1I ACTGG 4 cut(s) 140, 448, 488, 496
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 3 cut(s) 248, 311, 405
BseMII CTCAG 3 cut(s) 209, 231, 421
BseNI ACTGG 4 cut(s) 140, 448, 488, 496
BseRI GAGGAG 2 cut(s) 20, 405
Bsh1236I CGCG 2 cut(s) 199, 361
BshNI GGYRCC 2 cut(s) 336, 416
BsiHKAI GWGCWC 2 cut(s) 246, 408
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 1 cut(s) 252
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 246, 408, 498
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 106
BspCNI CTCAG 3 cut(s) 210, 232, 420
BspFNI CGCG 2 cut(s) 199, 361
BspLI GGNNCC 2 cut(s) 338, 418
BspT107I GGYRCC 2 cut(s) 336, 416
BsrI ACTGG 4 cut(s) 140, 448, 488, 496
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 1 cut(s) 460
BssNAI GTATAC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 101
Bst1107I GTATAC 1 cut(s) 374
Bst2UI CCWGG 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 4 cut(s) 218, 240, 332, 407
BstF5I GGATG 3 cut(s) 248, 311, 405
BstFNI CGCG 2 cut(s) 199, 361
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 2 cut(s) 17, 84
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 40
BstUI CGCG 2 cut(s) 199, 361
BstZ17I GTATAC 1 cut(s) 374
BtrI CACGTC 1 cut(s) 162
BtsCI GGATG 3 cut(s) 248, 311, 405
BtsIMutI CAGTG 2 cut(s) 415, 481
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 3 cut(s) 125, 138, 426
Csp6I GTAC 1 cut(s) 417
CviAII CATG 2 cut(s) 122, 473
CviJI RGCY 7 cut(s) 78, 211, 244, 385, 406, 496, 523
CviKI_1 RGCY 7 cut(s) 78, 211, 244, 385, 406, 496, 523
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 4 cut(s) 218, 240, 332, 407
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Ecl136II GAGCTC 2 cut(s) 244, 406
Eco130I CCWWGG 1 cut(s) 101
Eco24I GRGCYC 3 cut(s) 246, 408, 498
Eco47I GGWCC 3 cut(s) 125, 138, 426
Eco53kI GAGCTC 2 cut(s) 244, 406
Eco88I CYCGRG 1 cut(s) 377
EcoICRI GAGCTC 2 cut(s) 244, 406
EcoRI GAATTC 2 cut(s) 29, 449
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 101
EcoT38I GRGCYC 3 cut(s) 246, 408, 498
ErhI CCWWGG 1 cut(s) 101
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 2 cut(s) 125, 476
FatI CATG 2 cut(s) 121, 472
FblI GTMKAC 1 cut(s) 373
FokI GGATG 3 cut(s) 235, 318, 412
FriOI GRGCYC 3 cut(s) 246, 408, 498
GlaI GCGC 1 cut(s) 362
GsuI CTGGAG 1 cut(s) 465
HapII CCGG 1 cut(s) 252
HhaI GCGC 1 cut(s) 363
Hin1II CATG 2 cut(s) 125, 476
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HpaII CCGG 1 cut(s) 252
Hpy166II GTNNAC 4 cut(s) 37, 374, 470, 479
Hpy188I TCNGA 3 cut(s) 219, 241, 426
Hpy188III TCNNGA 2 cut(s) 303, 379
Hpy8I GTNNAC 4 cut(s) 37, 374, 470, 479
HpyCH4IV ACGT 1 cut(s) 161
HpyCH4V TGCA 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 84
HpyF3I CTNAG 4 cut(s) 218, 240, 332, 407
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 2 cut(s) 125, 476
HspAI GCGC 1 cut(s) 361
KpnI GGTACC 1 cut(s) 420
Kzo9I GATC 1 cut(s) 460
LmnI GCTCC 2 cut(s) 296, 403
LweI GCATC 1 cut(s) 308
MaeII ACGT 1 cut(s) 161
MaeIII GTNAC 1 cut(s) 108
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 1 cut(s) 449
MhlI GDGCHC 3 cut(s) 246, 408, 498
MluCI AATT 5 cut(s) 13, 29, 145, 168, 449
MnlI CCTC 4 cut(s) 138, 175, 215, 383
MslI CAYNNNNRTG 3 cut(s) 233, 304, 342
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 2 cut(s) 199, 361
MwoI GCNNNNNNNGC 2 cut(s) 17, 84
NdeII GATC 1 cut(s) 460
NlaIII CATG 2 cut(s) 125, 476
NlaIV GGNNCC 2 cut(s) 338, 418
NmeAIII GCCGAG 1 cut(s) 411
PaeR7I CTCGAG 1 cut(s) 377
PsiI TTATAA 1 cut(s) 153
Psp124BI GAGCTC 2 cut(s) 246, 408
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 2 cut(s) 338, 418
PspPI GGNCC 3 cut(s) 125, 138, 426
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
RseI CAYNNNNRTG 3 cut(s) 233, 304, 342
SacI GAGCTC 2 cut(s) 246, 408
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 3 cut(s) 125, 138, 426
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 3 cut(s) 246, 408, 498
SetI ASST 7 cut(s) 80, 143, 164, 186, 246, 408, 422
SfaNI GCATC 1 cut(s) 308
SfcI CTRYAG 1 cut(s) 40
Sfr274I CTCGAG 1 cut(s) 377
SinI GGWCC 3 cut(s) 125, 138, 426
SlaI CTCGAG 1 cut(s) 377
SmiMI CAYNNNNRTG 3 cut(s) 233, 304, 342
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 5 cut(s) 13, 29, 145, 168, 449
SsiI CCGC 1 cut(s) 106
SstI GAGCTC 2 cut(s) 246, 408
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 101
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 27, 378
TasI AATT 5 cut(s) 13, 29, 145, 168, 449
TscAI CASTG 2 cut(s) 415, 488
TspRI CASTG 2 cut(s) 415, 488
VpaK11BI GGWCC 3 cut(s) 125, 138, 426
XapI RAATTY 3 cut(s) 13, 29, 449
XcmI CCANNNNNNNNNTGG 2 cut(s) 138, 440
XhoI CTCGAG 1 cut(s) 377
XmiI GTMKAC 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.