FvH4_6g42850

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007174
Physical Location & Seq
Forward (+)
33284646 .. 33288093
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42850.t1

Sequence Viewer

Length: 846 bp
ATGCTAGCTGCGGTTGCTGTCAATTTGAGATCAGCAGCTTCCAGAGCCTTCTCCAAGTCTCTACCAAACTCAACTTTCTCACTCTCTCCTTTGCGTTCTAGGTTCTCGATTCGCGCCATGGCTGATTCTGCTGGGCCATTCAAGAAAGTCCAAATCCAGAGAGACGATACTGCATTTGATGCATATATTGTTGGCAAAGAAGATGCTCCTGGGATTGTTGTACTTCAAGAGTGGTGGGGTGTAGATTTTGAGATAAAGAACCACGCTGTCAAAATTTCTGAACTTGCTCCTGGATTCAAGGCACTTATCCCTGACTTGTATCGGGGAAAGGTTGGTTTGGATGTTGCTGAAGCACAACATTTGATGGATGGTCTTGACTGGCAAGGCGCGGTAAAGGATATCCAGGCTTCAGTTAATTGGCTGAAAGCAAATGGTTCGAAGAAGGTTGGTGTTACTGGATTTTGCATGGGGGGTGCTCTCTCAATTGCAAGTTCTGTTTTGGTCCCAGAGGTCGATGCTGTTGTTGCATTCTATGGAGTACCATCATCAGAGCTTGCTGATCCTGCTCAAGCTAAGGCTCCTATTCAGGCTCATTTTGGAGAGCTTGATAGTTTTGTTGGCTTTTCAGATGTAACGGCTGCCAAGTCTTTGGAGGAGAAGCTGAAAGCCTCAGGAGTCCCATATGAGGTGCACCTGTATCCAGGCAATGCCCATGCTTTCATGAATAGGTCTGAAGACGGTGTGAAGCGCAGAAAAGGCATGGGAATGCCTGATGAGGATGAAGCTGCAGTTCAGCTCGCTTGGTCTCGCTTCCAATCATGGATGGCCCGCCATTTGTCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

282

Amino Acids

30.28

Weight (kDa)

6.32

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 59 - 278 3.4e-58 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 114, 389
AciI CCGC 3 cut(s) 11, 389, 829
AclWI GGATC 1 cut(s) 554
AcsI RAATTY 1 cut(s) 273
AcuI CTGAAG 3 cut(s) 369, 393, 753
AfaI GTAC 2 cut(s) 222, 540
AfiI CCNNNNNNNGG 1 cut(s) 685
AgsI TTSAA 3 cut(s) 142, 227, 298
AjnI CCWGG 4 cut(s) 208, 289, 402, 700
AluBI AGCT 8 cut(s) 8, 38, 553, 572, 604, 661, 785, 796
AluI AGCT 8 cut(s) 8, 38, 553, 572, 604, 661, 785, 796
Alw21I GWGCWC 2 cut(s) 478, 693
Alw26I GTCTC 3 cut(s) 63, 156, 810
Alw44I GTGCAC 1 cut(s) 689
AlwI GGATC 1 cut(s) 554
AoxI GGCC 2 cut(s) 134, 825
ApaLI GTGCAC 1 cut(s) 689
ApeKI GCWGC 4 cut(s) 8, 35, 638, 785
ApoI RAATTY 1 cut(s) 273
AspLEI GCGC 3 cut(s) 116, 389, 750
AspS9I GGNCC 3 cut(s) 134, 502, 826
AsuII TTCGAA 1 cut(s) 437
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 502
AxyI CCTNAGG 1 cut(s) 670
BaeGI GKGCMC 1 cut(s) 693
BbsI GAAGAC 1 cut(s) 741
Bbv12I GWGCWC 2 cut(s) 478, 693
BbvI GCAGC 3 cut(s) 47, 625, 772
BccI CCATC 4 cut(s) 358, 362, 550, 817
BceAI ACGGC 1 cut(s) 651
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BciT130I CCWGG 4 cut(s) 210, 291, 404, 702
BciVI GTATCC 1 cut(s) 708
BcoDI GTCTC 3 cut(s) 63, 156, 810
BfaI CTAG 2 cut(s) 5, 99
BfmI CTRYAG 1 cut(s) 786
BfuI GTATCC 1 cut(s) 708
BisI GCNGC 4 cut(s) 9, 36, 639, 786
BlsI GCNGC 4 cut(s) 10, 37, 640, 787
Bme1390I CCNGG 4 cut(s) 210, 291, 404, 702
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 134, 502, 826
BmiI GGNNCC 2 cut(s) 504, 579
BmrFI CCNGG 4 cut(s) 210, 291, 404, 702
BmsI GCATC 3 cut(s) 169, 193, 505
BmtI GCTAGC 1 cut(s) 8
BpiI GAAGAC 1 cut(s) 741
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 437
BpuEI CTTGAG 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 810
BsaJI CCNNGG 2 cut(s) 117, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 685
Bse1I ACTGG 2 cut(s) 383, 460
Bse21I CCTNAGG 1 cut(s) 670
Bse3DI GCAATG 1 cut(s) 712
BseBI CCWGG 4 cut(s) 210, 291, 404, 702
BseDI CCNNGG 2 cut(s) 117, 209
BseGI GGATG 4 cut(s) 346, 373, 784, 828
BseLI CCNNNNNNNGG 1 cut(s) 685
BseMI GCAATG 1 cut(s) 712
BseMII CTCAG 1 cut(s) 684
BseNI ACTGG 2 cut(s) 383, 460
BseRI GAGGAG 1 cut(s) 668
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 3 cut(s) 47, 625, 772
BseYI CCCAGC 1 cut(s) 131
Bsh1236I CGCG 2 cut(s) 114, 389
BshFI GGCC 2 cut(s) 136, 827
BsiHKAI GWGCWC 2 cut(s) 478, 693
BslFI GGGAC 2 cut(s) 488, 662
BslI CCNNNNNNNGG 1 cut(s) 685
BsmAI GTCTC 3 cut(s) 63, 156, 810
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 2 cut(s) 488, 662
BsmI GAATGC 2 cut(s) 527, 771
BsnI GGCC 2 cut(s) 136, 827
Bso31I GGTCTC 1 cut(s) 810
Bsp119I TTCGAA 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 478, 693
Bsp143I GATC 2 cut(s) 29, 559
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 3 cut(s) 11, 389, 829
BspANI GGCC 2 cut(s) 136, 827
BspCNI CTCAG 1 cut(s) 683
BspFNI CGCG 2 cut(s) 114, 389
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 2 cut(s) 504, 579
BspMAI CTGCAG 1 cut(s) 790
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 554
BspT104I TTCGAA 1 cut(s) 437
BspTNI GGTCTC 1 cut(s) 810
BsrDI GCAATG 1 cut(s) 712
BsrI ACTGG 2 cut(s) 383, 460
BssECI CCNNGG 2 cut(s) 117, 209
BssMI GATC 2 cut(s) 29, 559
BssT1I CCWWGG 1 cut(s) 117
Bst2UI CCWGG 4 cut(s) 210, 291, 404, 702
Bst4CI ACNGT 1 cut(s) 740
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 437
BstC8I GCNNGC 4 cut(s) 6, 555, 798, 829
BstDEI CTNAG 2 cut(s) 573, 670
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 4 cut(s) 346, 373, 784, 828
BstFNI CGCG 2 cut(s) 114, 389
BstHHI GCGC 3 cut(s) 116, 389, 750
BstKTI GATC 2 cut(s) 32, 562
BstMAI GTCTC 3 cut(s) 63, 156, 810
BstMBI GATC 2 cut(s) 29, 559
BstMWI GCNNNNNNNGC 7 cut(s) 14, 44, 128, 179, 524, 563, 756
BstNI CCWGG 4 cut(s) 210, 291, 404, 702
BstSCI CCNGG 4 cut(s) 208, 289, 402, 700
BstSFI CTRYAG 1 cut(s) 786
BstSLI GKGCMC 1 cut(s) 693
BstUI CGCG 2 cut(s) 114, 389
BstV1I GCAGC 3 cut(s) 47, 625, 772
BstV2I GAAGAC 1 cut(s) 741
BstXI CCANNNNNNTGG 1 cut(s) 649
Bsu36I CCTNAGG 1 cut(s) 670
BsuI GTATCC 1 cut(s) 708
BsuRI GGCC 2 cut(s) 136, 827
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 4 cut(s) 346, 373, 784, 828
Cac8I GCNNGC 4 cut(s) 6, 555, 798, 829
CciI TCATGA 1 cut(s) 720
CfoI GCGC 3 cut(s) 116, 389, 750
Cfr13I GGNCC 3 cut(s) 134, 502, 826
Csp6I GTAC 2 cut(s) 221, 539
CspCI CAANNNNNGTGG 2 cut(s) 215, 250
CviAII CATG 6 cut(s) 118, 466, 713, 721, 760, 819
CviQI GTAC 2 cut(s) 221, 539
DdeI CTNAG 2 cut(s) 573, 670
DpnI GATC 2 cut(s) 31, 561
DpnII GATC 2 cut(s) 29, 559
Eco130I CCWWGG 1 cut(s) 117
Eco31I GGTCTC 1 cut(s) 810
Eco32I GATATC 1 cut(s) 400
Eco47I GGWCC 1 cut(s) 502
Eco57I CTGAAG 3 cut(s) 369, 393, 753
Eco81I CCTNAGG 1 cut(s) 670
EcoRII CCWGG 4 cut(s) 208, 289, 402, 700
EcoRV GATATC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 117
EcoT22I ATGCAT 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 156
FaeI CATG 6 cut(s) 121, 469, 716, 724, 763, 822
FaqI GGGAC 2 cut(s) 488, 662
FatI CATG 6 cut(s) 117, 465, 712, 720, 759, 818
FauI CCCGC 1 cut(s) 836
FauNDI CATATG 1 cut(s) 682
Fnu4HI GCNGC 4 cut(s) 9, 36, 639, 786
FokI GGATG 4 cut(s) 353, 380, 791, 835
Fsp4HI GCNGC 4 cut(s) 9, 36, 639, 786
FspBI CTAG 2 cut(s) 5, 99
GlaI GCGC 3 cut(s) 115, 388, 749
GluI GCNGC 4 cut(s) 9, 36, 639, 786
GsaI CCCAGC 1 cut(s) 135
HaeIII GGCC 2 cut(s) 136, 827
HhaI GCGC 3 cut(s) 116, 389, 750
Hin1II CATG 6 cut(s) 121, 469, 716, 724, 763, 822
Hin6I GCGC 3 cut(s) 114, 387, 748
HinP1I GCGC 3 cut(s) 114, 387, 748
HinfI GANTC 4 cut(s) 109, 125, 294, 675
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 4 cut(s) 280, 550, 628, 733
Hpy188III TCNNGA 8 cut(s) 42, 106, 142, 157, 227, 374, 672, 721
Hpy8I GTNNAC 1 cut(s) 691
HpyAV CCTTC 2 cut(s) 58, 436
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4V TGCA 7 cut(s) 173, 182, 465, 488, 527, 691, 788
HpyF10VI GCNNNNNNNGC 7 cut(s) 14, 44, 128, 179, 524, 563, 756
HpyF3I CTNAG 2 cut(s) 573, 670
Hsp92II CATG 6 cut(s) 121, 469, 716, 724, 763, 822
HspAI GCGC 3 cut(s) 114, 387, 748
Kzo9I GATC 2 cut(s) 29, 559
LmnI GCTCC 3 cut(s) 211, 292, 583
Lsp1109I GCAGC 3 cut(s) 47, 625, 772
LweI GCATC 3 cut(s) 169, 193, 505
MaeI CTAG 2 cut(s) 5, 99
MaeIII GTNAC 2 cut(s) 451, 631
MalI GATC 2 cut(s) 31, 561
MboI GATC 2 cut(s) 29, 559
MboII GAAGA 3 cut(s) 212, 451, 746
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 2 cut(s) 478, 693
MluCI AATT 4 cut(s) 22, 273, 415, 483
MlyI GAGTC 1 cut(s) 684
MnlI CCTC 5 cut(s) 502, 646, 679, 679, 769
Mph1103I ATGCAT 1 cut(s) 184
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 764
MspR9I CCNGG 4 cut(s) 210, 291, 404, 702
MunI CAATTG 1 cut(s) 483
Mva1269I GAATGC 2 cut(s) 527, 771
MvaI CCWGG 4 cut(s) 210, 291, 404, 702
MvnI CGCG 2 cut(s) 114, 389
MwoI GCNNNNNNNGC 7 cut(s) 14, 44, 128, 179, 524, 563, 756
NcoI CCATGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 682
NdeII GATC 2 cut(s) 29, 559
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 121, 469, 716, 724, 763, 822
NlaIV GGNNCC 2 cut(s) 504, 579
NsiI ATGCAT 1 cut(s) 184
NspV TTCGAA 1 cut(s) 437
PagI TCATGA 1 cut(s) 720
PctI GAATGC 2 cut(s) 527, 771
PfeI GAWTC 3 cut(s) 109, 125, 294
PfoI TCCNGGA 1 cut(s) 289
PkrI GCNGC 4 cut(s) 10, 37, 640, 787
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
Psp6I CCWGG 4 cut(s) 208, 289, 402, 700
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 4 cut(s) 208, 289, 402, 700
PspN4I GGNNCC 2 cut(s) 504, 579
PspPI GGNCC 3 cut(s) 134, 502, 826
PstI CTGCAG 1 cut(s) 790
RsaI GTAC 2 cut(s) 222, 540
RsaNI GTAC 2 cut(s) 221, 539
RseI CAYNNNNRTG 1 cut(s) 764
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 4 cut(s) 9, 36, 639, 786
Sau3AI GATC 2 cut(s) 29, 559
Sau96I GGNCC 3 cut(s) 134, 502, 826
SchI GAGTC 1 cut(s) 684
ScrFI CCNGG 4 cut(s) 210, 291, 404, 702
SduI GDGCHC 2 cut(s) 478, 693
SfaNI GCATC 3 cut(s) 169, 193, 505
SfcI CTRYAG 1 cut(s) 786
SfuI TTCGAA 1 cut(s) 437
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 567
SmoI CTYRAG 1 cut(s) 567
Sse9I AATT 4 cut(s) 22, 273, 415, 483
SsiI CCGC 3 cut(s) 11, 389, 829
SspMI CTAG 2 cut(s) 5, 99
StyD4I CCNGG 4 cut(s) 208, 289, 402, 700
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 740
TaqI TCGA 3 cut(s) 107, 437, 513
TasI AATT 4 cut(s) 22, 273, 415, 483
TatI WGTACW 1 cut(s) 220
TfiI GAWTC 3 cut(s) 109, 125, 294
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TseI GCWGC 4 cut(s) 8, 35, 638, 785
TspDTI ATGAA 3 cut(s) 709, 737, 795
VneI GTGCAC 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 1 cut(s) 273
XspI CTAG 2 cut(s) 5, 99
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.