MD17G1099200.v1.1

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007174
Physical Location & Seq
Reverse (-)
8429422 .. 8430671
1250 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1099200.v1.1.491

Sequence Viewer

Length: 354 bp
ATGCTGACTACTGTCGCTGCTGTTAGTTTGCGATCAGCAGCTTCCCGAGCCTTCTTCTTCTCCAAAGCTCTCCCAAACTCCGCCGCCACTTCCTTCTCGCTCGCACGGACGCTCTCTCCCTCCGCCGTTTCTCGCTCCTCATTTCGCGCCATGGCCTCCGACTCTGCTGCCCCCTTCAACAAAATCCAAATCCAAAGAGACGACACTACATTTGATGCGTATGTTGTTGGCAAAGACGGCGCTCCTGGGATTGTTGTGCTTCAAGAGTGGTGGGGTGTGGATTATGAAATTAAGAACCATGCTGTCAAGATTTCTCAACTTGCTCCTGGATTCAAAGCACTTATCCCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

118

Amino Acids

12.54

Weight (kDa)

9.52

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 71 - 117 1.4e-07 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 147
AciI CCGC 3 cut(s) 81, 84, 123
AgsI TTSAA 3 cut(s) 178, 263, 334
AjnI CCWGG 2 cut(s) 244, 325
AluBI AGCT 2 cut(s) 41, 68
AluI AGCT 2 cut(s) 41, 68
Alw26I GTCTC 1 cut(s) 192
Ama87I CYCGRG 1 cut(s) 45
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 3 cut(s) 17, 38, 167
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 2 cut(s) 149, 242
AvaI CYCGRG 1 cut(s) 45
BbvI GCAGC 3 cut(s) 4, 50, 154
BceAI ACGGC 2 cut(s) 110, 253
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 2 cut(s) 246, 327
BcoDI GTCTC 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 243
BisI GCNGC 4 cut(s) 18, 39, 84, 168
BlsI GCNGC 4 cut(s) 19, 40, 85, 169
Bme1390I CCNGG 2 cut(s) 246, 327
BmeT110I CYCGRG 1 cut(s) 45
BmrFI CCNGG 2 cut(s) 246, 327
BmsI GCATC 1 cut(s) 205
BoxI GACNNNNGTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 150, 245
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
BseBI CCWGG 2 cut(s) 246, 327
BseDI CCNNGG 2 cut(s) 150, 245
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 3 cut(s) 4, 50, 154
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 45
BsmAI GTCTC 1 cut(s) 192
BsmBI CGTCTC 1 cut(s) 192
BsnI GGCC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 45
Bsp143I GATC 1 cut(s) 32
Bsp19I CCATGG 1 cut(s) 150
BspACI CCGC 3 cut(s) 81, 84, 123
BspANI GGCC 1 cut(s) 155
BspFNI CGCG 1 cut(s) 147
BssECI CCNNGG 2 cut(s) 150, 245
BssMI GATC 1 cut(s) 32
BssT1I CCWWGG 1 cut(s) 150
Bst2UI CCWGG 2 cut(s) 246, 327
Bst4CI ACNGT 1 cut(s) 13
BstC8I GCNNGC 1 cut(s) 102
BstDSI CCRYGG 1 cut(s) 150
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 2 cut(s) 149, 242
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 1 cut(s) 32
BstMWI GCNNNNNNNGC 2 cut(s) 47, 237
BstNI CCWGG 2 cut(s) 246, 327
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 2 cut(s) 244, 325
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 3 cut(s) 4, 50, 154
BsuRI GGCC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 150
Cac8I GCNNGC 1 cut(s) 102
CfoI GCGC 2 cut(s) 149, 242
CseI GACGC 1 cut(s) 118
CspCI CAANNNNNGTGG 2 cut(s) 251, 286
CviAII CATG 2 cut(s) 151, 299
CviJI RGCY 4 cut(s) 41, 50, 68, 155
CviKI_1 RGCY 4 cut(s) 41, 50, 68, 155
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
EciI GGCGGA 2 cut(s) 70, 112
Eco130I CCWWGG 1 cut(s) 150
Eco88I CYCGRG 1 cut(s) 45
EcoRII CCWGG 2 cut(s) 244, 325
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 2 cut(s) 154, 302
FaiI YATR 4 cut(s) 152, 222, 285, 300
FatI CATG 2 cut(s) 150, 298
Fnu4HI GCNGC 4 cut(s) 18, 39, 84, 168
Fsp4HI GCNGC 4 cut(s) 18, 39, 84, 168
GlaI GCGC 2 cut(s) 148, 241
GluI GCNGC 4 cut(s) 18, 39, 84, 168
HaeII RGCGCY 1 cut(s) 243
HaeIII GGCC 1 cut(s) 155
HgaI GACGC 1 cut(s) 118
HhaI GCGC 2 cut(s) 149, 242
Hin1II CATG 2 cut(s) 154, 302
Hin6I GCGC 2 cut(s) 147, 240
HinP1I GCGC 2 cut(s) 147, 240
HinfI GANTC 2 cut(s) 161, 330
Hpy188I TCNGA 1 cut(s) 160
Hpy188III TCNNGA 3 cut(s) 45, 263, 307
HpyAV CCTTC 3 cut(s) 61, 103, 184
HpyCH4III ACNGT 1 cut(s) 13
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 237
Hsp92II CATG 2 cut(s) 154, 302
HspAI GCGC 2 cut(s) 147, 240
Kzo9I GATC 1 cut(s) 32
LmnI GCTCC 3 cut(s) 140, 247, 328
LpnPI CCDG 4 cut(s) 231, 258, 312, 339
Lsp1109I GCAGC 3 cut(s) 4, 50, 154
LweI GCATC 1 cut(s) 205
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MboII GAAGA 2 cut(s) 46, 49
MluCI AATT 1 cut(s) 288
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 3 cut(s) 130, 148, 166
MseI TTAA 1 cut(s) 291
MspR9I CCNGG 2 cut(s) 246, 327
MvaI CCWGG 2 cut(s) 246, 327
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 2 cut(s) 47, 237
NcoI CCATGG 1 cut(s) 150
NdeII GATC 1 cut(s) 32
NlaIII CATG 2 cut(s) 154, 302
PfeI GAWTC 1 cut(s) 330
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 4 cut(s) 19, 40, 85, 169
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PshAI GACNNNNGTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 244, 325
PspGI CCWGG 2 cut(s) 244, 325
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 4 cut(s) 18, 39, 84, 168
Sau3AI GATC 1 cut(s) 32
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 2 cut(s) 246, 327
SetI ASST 2 cut(s) 43, 70
SfaNI GCATC 1 cut(s) 205
Sse9I AATT 1 cut(s) 288
SsiI CCGC 3 cut(s) 81, 84, 123
StyD4I CCNGG 2 cut(s) 244, 325
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 13
TasI AATT 1 cut(s) 288
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseI GCWGC 3 cut(s) 17, 38, 167
TspDTI ATGAA 1 cut(s) 300
TspGWI ACGGA 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.