MD17G1099100.v1.1

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007174
Physical Location & Seq
Reverse (-)
8419022 .. 8420468
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1099100.v1.1.491

Sequence Viewer

Length: 381 bp
ATGGGTGGTGCTCTCTCAATTGCAAGTTCTGTTTTGGTCCCTGAAGTTGATGCTGTTGTTGCATTTTATGGTGTGCCGTCGTCAGAGCTTGCAGACCCAGCCAAAGCTAAGGCTCCTGTTCAGGCTCATTTTGGAGAGCTTGATAGTTTCGTTGGCTTTTCAGACGTGACGGCTGCCAAATCTTTGGAGGAGAAGCTGAAAGCGTCAGGAATCCCTTACGAGGTGCACATTTATCCCGGCAATGCCCATGCGTTCATGAATAGGTCTCCAGAAGCTGTGAAGAGGAGGAAGGGCATGGGACTGGCTGATGAGGATGAAGCAGCATGTCAGCTGGCCTGGTCTCGCTTCCAGTCATGGATGTCTTGTTATTTGCCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

127

Amino Acids

13.45

Weight (kDa)

5.28

Isoelectric Point (pI)

60.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DLH PF01738 1 - 123 2e-28 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 63
AfiI CCNNNNNNNGG 1 cut(s) 220
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 1 cut(s) 335
AluBI AGCT 6 cut(s) 88, 107, 139, 196, 275, 331
AluI AGCT 6 cut(s) 88, 107, 139, 196, 275, 331
Alw21I GWGCWC 2 cut(s) 13, 228
Alw26I GTCTC 2 cut(s) 270, 345
Alw44I GTGCAC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 275
AoxI GGCC 1 cut(s) 333
ApaLI GTGCAC 1 cut(s) 224
ApeKI GCWGC 2 cut(s) 173, 320
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 37
BaeGI GKGCMC 1 cut(s) 228
Bbv12I GWGCWC 2 cut(s) 13, 228
BbvI GCAGC 2 cut(s) 160, 332
BceAI ACGGC 2 cut(s) 61, 186
BciT130I CCWGG 1 cut(s) 337
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 2 cut(s) 270, 345
BisI GCNGC 2 cut(s) 174, 321
BlsI GCNGC 2 cut(s) 175, 322
Bme1390I CCNGG 2 cut(s) 237, 337
Bme18I GGWCC 1 cut(s) 37
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 39, 114
BmrFI CCNGG 2 cut(s) 237, 337
BmsI GCATC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 108
BpuMI CCSGG 1 cut(s) 237
BsaI GGTCTC 2 cut(s) 270, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse1I ACTGG 2 cut(s) 306, 349
Bse3DI GCAATG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 337
BseGI GGATG 2 cut(s) 319, 363
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMI GCAATG 1 cut(s) 247
BseNI ACTGG 2 cut(s) 306, 349
BseRI GAGGAG 2 cut(s) 203, 298
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 2 cut(s) 160, 332
BseYI CCCAGC 1 cut(s) 97
BshFI GGCC 1 cut(s) 335
BsiHKAI GWGCWC 2 cut(s) 13, 228
BsiSI CCGG 1 cut(s) 237
BslFI GGGAC 2 cut(s) 23, 312
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 2 cut(s) 270, 345
BsmFI GGGAC 2 cut(s) 23, 312
BsnI GGCC 1 cut(s) 335
Bso31I GGTCTC 2 cut(s) 270, 345
Bsp1286I GDGCHC 2 cut(s) 13, 228
BspACI CCGC 1 cut(s) 375
BspANI GGCC 1 cut(s) 335
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 39, 114
BspTNI GGTCTC 2 cut(s) 270, 345
BsrDI GCAATG 1 cut(s) 247
BsrI ACTGG 2 cut(s) 306, 349
Bst2UI CCWGG 1 cut(s) 337
Bst6I CTCTTC 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 90, 333, 375
BstDEI CTNAG 1 cut(s) 108
BstF5I GGATG 2 cut(s) 319, 363
BstMAI GTCTC 2 cut(s) 270, 345
BstMWI GCNNNNNNNGC 2 cut(s) 59, 98
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 2 cut(s) 235, 335
BstSLI GKGCMC 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 160, 332
BstXI CCANNNNNNTGG 1 cut(s) 184
BsuRI GGCC 1 cut(s) 335
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 2 cut(s) 319, 363
Cac8I GCNNGC 3 cut(s) 90, 333, 375
CaiI CAGNNNCTG 1 cut(s) 275
CciI TCATGA 1 cut(s) 255
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 1 cut(s) 192
CviAII CATG 5 cut(s) 248, 256, 295, 324, 354
DdeI CTNAG 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Eco31I GGTCTC 2 cut(s) 270, 345
Eco47I GGWCC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 63
EcoRII CCWGG 1 cut(s) 335
FaeI CATG 5 cut(s) 251, 259, 298, 327, 357
FaiI YATR 6 cut(s) 69, 249, 257, 296, 325, 355
FaqI GGGAC 2 cut(s) 23, 312
FatI CATG 5 cut(s) 247, 255, 294, 323, 353
Fnu4HI GCNGC 2 cut(s) 174, 321
FokI GGATG 2 cut(s) 326, 370
Fsp4HI GCNGC 2 cut(s) 174, 321
GluI GCNGC 2 cut(s) 174, 321
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 1 cut(s) 335
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 192
Hin1II CATG 5 cut(s) 251, 259, 298, 327, 357
HinfI GANTC 1 cut(s) 210
HpaII CCGG 1 cut(s) 237
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 2 cut(s) 85, 163
Hpy188III TCNNGA 3 cut(s) 207, 256, 269
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 4 cut(s) 23, 62, 92, 226
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 98
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 1 cut(s) 165
Hsp92II CATG 5 cut(s) 251, 259, 298, 327, 357
LmnI GCTCC 1 cut(s) 118
Lsp1109I GCAGC 2 cut(s) 160, 332
LweI GCATC 1 cut(s) 40
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 1 cut(s) 166
MboII GAAGA 1 cut(s) 292
MfeI CAATTG 1 cut(s) 18
MhlI GDGCHC 2 cut(s) 13, 228
MluCI AATT 1 cut(s) 18
MnlI CCTC 5 cut(s) 181, 214, 276, 279, 304
MseI TTAA 1 cut(s) 379
MspA1I CMGCKG 1 cut(s) 331
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 2 cut(s) 237, 337
MunI CAATTG 1 cut(s) 18
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 2 cut(s) 59, 98
NciI CCSGG 1 cut(s) 237
NlaIII CATG 5 cut(s) 251, 259, 298, 327, 357
NlaIV GGNNCC 2 cut(s) 39, 114
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 327
PagI TCATGA 1 cut(s) 255
PfeI GAWTC 1 cut(s) 210
PkrI GCNGC 2 cut(s) 175, 322
Psp6I CCWGG 1 cut(s) 335
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 2 cut(s) 39, 114
PspPI GGNCC 1 cut(s) 37
PstNI CAGNNNCTG 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 331
SaqAI TTAA 1 cut(s) 379
SatI GCNGC 2 cut(s) 174, 321
Sau96I GGNCC 1 cut(s) 37
ScrFI CCNGG 2 cut(s) 237, 337
SduI GDGCHC 2 cut(s) 13, 228
SetI ASST 9 cut(s) 90, 109, 141, 168, 198, 225, 266, 277, 333
SfaNI GCATC 1 cut(s) 40
SinI GGWCC 1 cut(s) 37
Sse9I AATT 1 cut(s) 18
SsiI CCGC 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 235, 335
TaiI ACGT 1 cut(s) 168
TasI AATT 1 cut(s) 18
TfiI GAWTC 1 cut(s) 210
Tru1I TTAA 1 cut(s) 379
Tru9I TTAA 1 cut(s) 379
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 2 cut(s) 173, 320
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 3 cut(s) 244, 272, 330
VneI GTGCAC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 37
XceI RCATGY 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.