Rmu_sc0038467.1_g000003

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0038467.1
OG0007174
Physical Location & Seq
Forward (+)
1918 .. 3918
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0038467.1_g000003.1.cds

Sequence Viewer

Length: 573 bp
atggcggattctgctgccccgttcaagaaagtccaaatccagagagacgataccgcatttgatgcatatgttgttggcaaagaaggtgctcctgggattgtcgtagttcaagagtggtggggtgtagattttgaaataaagaaccatgctgtcaaaatttctgaacttgctcctggattcaaagcacttatccctgacttgtatcggggaaaggttggtttggatgttgcagaagcacaacatttgatggaaggtcttgactggcaagctgcagtaaaggatatccaggcttcagttaattggctgaaagcaaatggttcaaagaaggttggagttactggattctgcatgggaggtgctctctcaattgcaagttctgttttggtccctgaggtcgatgctgttgttgcattttatggaataccatcatcagagcttgcagaccctgcaaaagctaaggctcctattcaggctcattttggagagcttgatagttttgttggcttttcagatgtcacggctcaatttgttgggcaccttggttgtgattttggaaaaaggctcattggataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

190

Amino Acids

20.16

Weight (kDa)

5.15

Isoelectric Point (pI)

13.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 534
AciI CCGC 2 cut(s) 5, 54
AcsI RAATTY 1 cut(s) 156
AcuI CTGAAG 1 cut(s) 276
AgsI TTSAA 5 cut(s) 25, 110, 134, 181, 321
AjnI CCWGG 3 cut(s) 91, 172, 285
AluBI AGCT 4 cut(s) 269, 436, 455, 487
AluI AGCT 4 cut(s) 269, 436, 455, 487
Alw21I GWGCWC 2 cut(s) 91, 361
Alw26I GTCTC 1 cut(s) 39
AlwNI CAGNNNCTG 1 cut(s) 446
ApeKI GCWGC 2 cut(s) 14, 269
ApoI RAATTY 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 385
AvaII GGWCC 1 cut(s) 385
AxyI CCTNAGG 1 cut(s) 390
BaeGI GKGCMC 1 cut(s) 537
BanI GGYRCC 1 cut(s) 534
Bbv12I GWGCWC 2 cut(s) 91, 361
BbvI GCAGC 1 cut(s) 256
BccI CCATC 2 cut(s) 241, 433
BceAI ACGGC 1 cut(s) 534
BciT130I CCWGG 3 cut(s) 93, 174, 287
BcoDI GTCTC 1 cut(s) 39
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 2 cut(s) 15, 270
BlsI GCNGC 2 cut(s) 16, 271
Bme1390I CCNGG 3 cut(s) 93, 174, 287
Bme18I GGWCC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 385
BmiI GGNNCC 3 cut(s) 387, 462, 536
BmrFI CCNGG 3 cut(s) 93, 174, 287
BmsI GCATC 2 cut(s) 52, 388
BplI GAGNNNNNCTC 2 cut(s) 345, 377
Bpu10I CCTNAGC 1 cut(s) 456
BsaJI CCNNGG 2 cut(s) 92, 538
Bse1I ACTGG 2 cut(s) 266, 343
Bse21I CCTNAGG 1 cut(s) 390
BseBI CCWGG 3 cut(s) 93, 174, 287
BseDI CCNNGG 2 cut(s) 92, 538
BseGI GGATG 1 cut(s) 229
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 2 cut(s) 266, 343
BseSI GKGCMC 1 cut(s) 537
BseXI GCAGC 1 cut(s) 256
BshNI GGYRCC 1 cut(s) 534
BsiHKAI GWGCWC 2 cut(s) 91, 361
BslFI GGGAC 1 cut(s) 371
BsmAI GTCTC 1 cut(s) 39
BsmBI CGTCTC 1 cut(s) 39
BsmFI GGGAC 1 cut(s) 371
Bsp1286I GDGCHC 3 cut(s) 91, 361, 537
BspACI CCGC 2 cut(s) 5, 54
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 387, 462, 536
BspMAI CTGCAG 1 cut(s) 274
BspT107I GGYRCC 1 cut(s) 534
BsrI ACTGG 2 cut(s) 266, 343
BssECI CCNNGG 2 cut(s) 92, 538
BssT1I CCWWGG 1 cut(s) 538
Bst2UI CCWGG 3 cut(s) 93, 174, 287
BstAPI GCANNNNNTGC 2 cut(s) 62, 446
BstC8I GCNNGC 2 cut(s) 267, 438
BstDEI CTNAG 2 cut(s) 390, 456
BstF5I GGATG 1 cut(s) 229
BstMAI GTCTC 1 cut(s) 39
BstMWI GCNNNNNNNGC 4 cut(s) 11, 62, 407, 446
BstNI CCWGG 3 cut(s) 93, 174, 287
BstSCI CCNGG 3 cut(s) 91, 172, 285
BstSFI CTRYAG 1 cut(s) 270
BstSLI GKGCMC 1 cut(s) 537
BstV1I GCAGC 1 cut(s) 256
Bsu36I CCTNAGG 1 cut(s) 390
BtsCI GGATG 1 cut(s) 229
Cac8I GCNNGC 2 cut(s) 267, 438
CaiI CAGNNNCTG 1 cut(s) 446
Cfr13I GGNCC 1 cut(s) 385
CspCI CAANNNNNGTGG 2 cut(s) 98, 133
CviAII CATG 2 cut(s) 146, 349
DdeI CTNAG 2 cut(s) 390, 456
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 538
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 276
Eco81I CCTNAGG 1 cut(s) 390
EcoRII CCWGG 3 cut(s) 91, 172, 285
EcoRV GATATC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 538
EcoT22I ATGCAT 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 538
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 2 cut(s) 149, 352
FaiI YATR 5 cut(s) 67, 69, 147, 350, 417
FaqI GGGAC 1 cut(s) 371
FatI CATG 2 cut(s) 145, 348
FauNDI CATATG 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 15, 270
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 15, 270
GluI GCNGC 2 cut(s) 15, 270
Hin1II CATG 2 cut(s) 149, 352
HinfI GANTC 3 cut(s) 8, 177, 342
Hpy188I TCNGA 3 cut(s) 163, 433, 511
Hpy188III TCNNGA 4 cut(s) 25, 40, 110, 257
HpyAV CCTTC 3 cut(s) 77, 245, 319
HpyCH4V TGCA 8 cut(s) 65, 230, 272, 348, 371, 410, 440, 449
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 62, 407, 446
HpyF3I CTNAG 2 cut(s) 390, 456
Hsp92II CATG 2 cut(s) 149, 352
LmnI GCTCC 3 cut(s) 94, 175, 466
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 2 cut(s) 52, 388
MaeIII GTNAC 2 cut(s) 334, 514
MfeI CAATTG 1 cut(s) 366
MhlI GDGCHC 3 cut(s) 91, 361, 537
MluCI AATT 4 cut(s) 156, 298, 366, 524
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 2 cut(s) 347, 385
Mph1103I ATGCAT 1 cut(s) 67
MseI TTAA 1 cut(s) 297
MspR9I CCNGG 3 cut(s) 93, 174, 287
MunI CAATTG 1 cut(s) 366
MvaI CCWGG 3 cut(s) 93, 174, 287
MwoI GCNNNNNNNGC 4 cut(s) 11, 62, 407, 446
NdeI CATATG 1 cut(s) 67
NlaIII CATG 2 cut(s) 149, 352
NlaIV GGNNCC 3 cut(s) 387, 462, 536
NmuCI GTSAC 1 cut(s) 514
NsiI ATGCAT 1 cut(s) 67
PfeI GAWTC 3 cut(s) 8, 177, 342
PfoI TCCNGGA 1 cut(s) 172
PkrI GCNGC 2 cut(s) 16, 271
Psp6I CCWGG 3 cut(s) 91, 172, 285
PspGI CCWGG 3 cut(s) 91, 172, 285
PspN4I GGNNCC 3 cut(s) 387, 462, 536
PspPI GGNCC 1 cut(s) 385
PstI CTGCAG 1 cut(s) 274
PstNI CAGNNNCTG 1 cut(s) 446
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 2 cut(s) 15, 270
Sau96I GGNCC 1 cut(s) 385
ScrFI CCNGG 3 cut(s) 93, 174, 287
SduI GDGCHC 3 cut(s) 91, 361, 537
SfaNI GCATC 2 cut(s) 52, 388
SfcI CTRYAG 1 cut(s) 270
SinI GGWCC 1 cut(s) 385
Sse9I AATT 4 cut(s) 156, 298, 366, 524
SsiI CCGC 2 cut(s) 5, 54
StyD4I CCNGG 3 cut(s) 91, 172, 285
StyI CCWWGG 1 cut(s) 538
TaqI TCGA 1 cut(s) 396
TasI AATT 4 cut(s) 156, 298, 366, 524
TfiI GAWTC 3 cut(s) 8, 177, 342
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 2 cut(s) 14, 269
Tsp45I GTSAC 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.