Prupe.3G214600_v2.0.a1

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007174
Physical Location & Seq
Forward (+)
21792655 .. 21795864
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G214600.1

Sequence Viewer

Length: 861 bp
ATGTTGGCATCGGCTGCTATTAATCTGAGATCATCATCAGCTTCCAGAGCCTTCTCCAAAGCTCTCCCAAACTCCACAGCCGCTCCTTTCTCACTCGTACGGACTCTCTCTCGCTTCTCAGTCCGCTCTATGGCCGACTCTGCTGCTGCCCCATTCAAGAAAATCCAAATCCACAGAGGTGATACTGCATTTGATGCATATGTTGTTGGAAAAGAAGATGCTCCTGGGATTGTTGTACTTCAAGAGTGGTGGGGTGTGGATTTTGAAATCAAGAACCATGCTATGAAAATTTCTCAACTTGCTCCTGGTTTCAAAGCACTTATCCCAGACTTGTATCGGGGAAAGGTTGGTTTGGATGTTGCAGAAGCACAACATTTGATGGATGGTCTTGATTGGCAAGGTGCTGTAAAGGATATCCAAGCTTCTGCTAATTGGCTGAAAGCAAATGGTTCAAAGAAGGTCGGTGTTACTGGATTTTGTTTGGGGGGTGCTCTCTCAATTGCAAGTTCTGTTTTGGTCCCTGAGGTTGATGCTGTTGTTGCATTTTACGGAGTACCGTCATCAGAGCTTGCAGACCCTGCCAAAGCTAAGGCTCCTGTTCAGGCTCATTTTGGAGAGCTTGATAATTATGTTGGCTTCTCAGATGTGGGGGCTGCCAAGTCTTTGGAAGAAAAGCTGAAAGCATCAGGAATCCCGTATGAGGTGCACATTTATCCAGGCAATGGGCATGCCTTCATGAATAGATCTCAAGAAGGTGTGAAGAGGAGGAAGAACATGGGCATGCCTGATGAGGATGAAGCTGCAGTTCAGCTGGCTTGGTCTCGCTTCGAGTCGTGGATGACTCGTTATCTGTCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

287

Amino Acids

30.76

Weight (kDa)

7.81

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 2 cut(s) 83, 126
AciI CCGC 2 cut(s) 81, 124
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 288
AfaI GTAC 3 cut(s) 99, 237, 555
AfiI CCNNNNNNNGG 3 cut(s) 130, 700, 722
AgsI TTSAA 5 cut(s) 157, 242, 266, 313, 453
AjnI CCWGG 3 cut(s) 223, 304, 715
AleI CACNNNNGTG 1 cut(s) 177
AluBI AGCT 9 cut(s) 41, 62, 422, 568, 587, 619, 676, 800, 811
AluI AGCT 9 cut(s) 41, 62, 422, 568, 587, 619, 676, 800, 811
Alw21I GWGCWC 2 cut(s) 493, 708
Alw26I GTCTC 1 cut(s) 825
Alw44I GTGCAC 1 cut(s) 704
AlwNI CAGNNNCTG 1 cut(s) 578
AoxI GGCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 704
ApeKI GCWGC 5 cut(s) 14, 143, 146, 653, 800
ApoI RAATTY 1 cut(s) 288
AseI ATTAAT 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 517
AsuHPI GGTGA 1 cut(s) 191
AvaII GGWCC 1 cut(s) 517
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 708
Bbv12I GWGCWC 2 cut(s) 493, 708
BbvI GCAGC 4 cut(s) 130, 133, 640, 787
BccI CCATC 2 cut(s) 373, 377
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 3 cut(s) 225, 306, 717
BcoDI GTCTC 1 cut(s) 825
BfmI CTRYAG 1 cut(s) 801
BglII AGATCT 1 cut(s) 743
BisI GCNGC 6 cut(s) 15, 81, 144, 147, 654, 801
BlsI GCNGC 6 cut(s) 16, 82, 145, 148, 655, 802
Bme1390I CCNGG 3 cut(s) 225, 306, 717
Bme18I GGWCC 1 cut(s) 517
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 2 cut(s) 519, 594
BmrFI CCNGG 3 cut(s) 225, 306, 717
BmsI GCATC 5 cut(s) 17, 184, 208, 520, 692
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 1 cut(s) 732
BsaBI GATNNNNATC 1 cut(s) 34
BsaI GGTCTC 1 cut(s) 825
BsaJI CCNNGG 1 cut(s) 224
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 700, 722
Bse1I ACTGG 1 cut(s) 475
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 727
Bse8I GATNNNNATC 1 cut(s) 34
BseBI CCWGG 3 cut(s) 225, 306, 717
BseDI CCNNGG 1 cut(s) 224
BseGI GGATG 4 cut(s) 361, 388, 799, 843
BseJI GATNNNNATC 1 cut(s) 34
BseLI CCNNNNNNNGG 3 cut(s) 130, 700, 722
BseMI GCAATG 1 cut(s) 727
BseMII CTCAG 4 cut(s) 17, 132, 513, 654
BseNI ACTGG 1 cut(s) 475
BseRI GAGGAG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 4 cut(s) 130, 133, 640, 787
BshFI GGCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 493, 708
BsiWI CGTACG 1 cut(s) 97
BslFI GGGAC 1 cut(s) 503
BslI CCNNNNNNNGG 3 cut(s) 130, 700, 722
BsmAI GTCTC 1 cut(s) 825
BsmFI GGGAC 1 cut(s) 503
BsnI GGCC 1 cut(s) 134
Bso31I GGTCTC 1 cut(s) 825
Bsp1286I GDGCHC 2 cut(s) 493, 708
Bsp143I GATC 2 cut(s) 29, 743
BspACI CCGC 2 cut(s) 81, 124
BspANI GGCC 1 cut(s) 134
BspCNI CTCAG 4 cut(s) 18, 131, 514, 653
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 519, 594
BspMAI CTGCAG 1 cut(s) 805
BspTNI GGTCTC 1 cut(s) 825
BsrBI CCGCTC 2 cut(s) 83, 126
BsrDI GCAATG 1 cut(s) 727
BsrI ACTGG 1 cut(s) 475
BssECI CCNNGG 1 cut(s) 224
BssMI GATC 2 cut(s) 29, 743
Bst2UI CCWGG 3 cut(s) 225, 306, 717
Bst4CI ACNGT 1 cut(s) 558
Bst6I CTCTTC 1 cut(s) 755
BstAPI GCANNNNNTGC 3 cut(s) 14, 194, 578
BstC8I GCNNGC 4 cut(s) 570, 729, 782, 813
BstDEI CTNAG 5 cut(s) 26, 118, 522, 588, 640
BstF5I GGATG 4 cut(s) 361, 388, 799, 843
BstKTI GATC 2 cut(s) 32, 746
BstMAI GTCTC 1 cut(s) 825
BstMBI GATC 2 cut(s) 29, 743
BstMWI GCNNNNNNNGC 6 cut(s) 14, 47, 140, 194, 539, 578
BstNI CCWGG 3 cut(s) 225, 306, 717
BstNSI RCATGY 2 cut(s) 731, 784
BstSCI CCNGG 3 cut(s) 223, 304, 715
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 1 cut(s) 708
BstV1I GCAGC 4 cut(s) 130, 133, 640, 787
BstX2I RGATCY 1 cut(s) 743
BstXI CCANNNNNNTGG 1 cut(s) 664
BstYI RGATCY 1 cut(s) 743
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 134
BtsCI GGATG 4 cut(s) 361, 388, 799, 843
Cac8I GCNNGC 4 cut(s) 570, 729, 782, 813
CaiI CAGNNNCTG 1 cut(s) 578
CciI TCATGA 1 cut(s) 735
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 3 cut(s) 98, 236, 554
CspCI CAANNNNNGTGG 2 cut(s) 230, 265
CviAII CATG 5 cut(s) 278, 728, 736, 775, 781
CviQI GTAC 3 cut(s) 98, 236, 554
DdeI CTNAG 5 cut(s) 26, 118, 522, 588, 640
DpnI GATC 2 cut(s) 31, 745
DpnII GATC 2 cut(s) 29, 743
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 755
EarI CTCTTC 1 cut(s) 755
Eco31I GGTCTC 1 cut(s) 825
Eco32I GATATC 1 cut(s) 415
Eco47I GGWCC 1 cut(s) 517
Eco81I CCTNAGG 1 cut(s) 522
EcoRII CCWGG 3 cut(s) 223, 304, 715
EcoRV GATATC 1 cut(s) 415
EcoT22I ATGCAT 1 cut(s) 199
FaeI CATG 5 cut(s) 281, 731, 739, 778, 784
FaqI GGGAC 1 cut(s) 503
FatI CATG 5 cut(s) 277, 727, 735, 774, 780
FauNDI CATATG 1 cut(s) 199
Fnu4HI GCNGC 6 cut(s) 15, 81, 144, 147, 654, 801
FokI GGATG 4 cut(s) 368, 395, 806, 850
Fsp4HI GCNGC 6 cut(s) 15, 81, 144, 147, 654, 801
GluI GCNGC 6 cut(s) 15, 81, 144, 147, 654, 801
HaeIII GGCC 1 cut(s) 134
Hin1II CATG 5 cut(s) 281, 731, 739, 778, 784
HindIII AAGCTT 1 cut(s) 420
HinfI GANTC 5 cut(s) 103, 137, 690, 830, 841
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 1 cut(s) 706
Hpy188I TCNGA 3 cut(s) 27, 565, 643
Hpy188III TCNNGA 8 cut(s) 45, 157, 242, 271, 389, 687, 736, 749
Hpy8I GTNNAC 1 cut(s) 706
HpyAV CCTTC 4 cut(s) 61, 451, 742, 746
HpyCH4III ACNGT 1 cut(s) 558
HpyCH4V TGCA 8 cut(s) 188, 197, 362, 503, 542, 572, 706, 803
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 47, 140, 194, 539, 578
HpyF3I CTNAG 5 cut(s) 26, 118, 522, 588, 640
Hsp92II CATG 5 cut(s) 281, 731, 739, 778, 784
Kzo9I GATC 2 cut(s) 29, 743
LmnI GCTCC 4 cut(s) 88, 226, 307, 598
Lsp1109I GCAGC 4 cut(s) 130, 133, 640, 787
LweI GCATC 5 cut(s) 17, 184, 208, 520, 692
MaeIII GTNAC 1 cut(s) 466
MalI GATC 2 cut(s) 31, 745
MbiI CCGCTC 2 cut(s) 83, 126
MboI GATC 2 cut(s) 29, 743
MboII GAAGA 4 cut(s) 227, 680, 772, 781
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 743
MhlI GDGCHC 2 cut(s) 493, 708
MluCI AATT 4 cut(s) 288, 430, 498, 625
MlyI GAGTC 4 cut(s) 97, 131, 835, 839
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 6 cut(s) 170, 517, 694, 756, 759, 784
Mph1103I ATGCAT 1 cut(s) 199
MseI TTAA 2 cut(s) 21, 859
MslI CAYNNNNRTG 2 cut(s) 177, 779
MspA1I CMGCKG 1 cut(s) 811
MspR9I CCNGG 3 cut(s) 225, 306, 717
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 3 cut(s) 225, 306, 717
MwoI GCNNNNNNNGC 6 cut(s) 14, 47, 140, 194, 539, 578
NdeI CATATG 1 cut(s) 199
NdeII GATC 2 cut(s) 29, 743
NlaIII CATG 5 cut(s) 281, 731, 739, 778, 784
NlaIV GGNNCC 2 cut(s) 519, 594
NsiI ATGCAT 1 cut(s) 199
NspI RCATGY 2 cut(s) 731, 784
OliI CACNNNNGTG 1 cut(s) 177
PaeI GCATGC 2 cut(s) 731, 784
PagI TCATGA 1 cut(s) 735
PfeI GAWTC 1 cut(s) 690
Pfl23II CGTACG 1 cut(s) 97
PflMI CCANNNNNTGG 1 cut(s) 722
PkrI GCNGC 6 cut(s) 16, 82, 145, 148, 655, 802
PleI GAGTC 4 cut(s) 97, 131, 835, 838
PpsI GAGTC 4 cut(s) 97, 131, 835, 838
PshBI ATTAAT 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 223, 304, 715
PspGI CCWGG 3 cut(s) 223, 304, 715
PspLI CGTACG 1 cut(s) 97
PspN4I GGNNCC 2 cut(s) 519, 594
PspPI GGNCC 1 cut(s) 517
PstI CTGCAG 1 cut(s) 805
PstNI CAGNNNCTG 1 cut(s) 578
PsuI RGATCY 1 cut(s) 743
PvuII CAGCTG 1 cut(s) 811
RsaI GTAC 3 cut(s) 99, 237, 555
RsaNI GTAC 3 cut(s) 98, 236, 554
RseI CAYNNNNRTG 2 cut(s) 177, 779
SaqAI TTAA 2 cut(s) 21, 859
SatI GCNGC 6 cut(s) 15, 81, 144, 147, 654, 801
Sau3AI GATC 2 cut(s) 29, 743
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 4 cut(s) 97, 131, 835, 839
ScrFI CCNGG 3 cut(s) 225, 306, 717
SduI GDGCHC 2 cut(s) 493, 708
SfaNI GCATC 5 cut(s) 17, 184, 208, 520, 692
SfcI CTRYAG 1 cut(s) 801
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 177, 779
SmlI CTYRAG 1 cut(s) 747
SmoI CTYRAG 1 cut(s) 747
SphI GCATGC 2 cut(s) 731, 784
Sse9I AATT 4 cut(s) 288, 430, 498, 625
SsiI CCGC 2 cut(s) 81, 124
StyD4I CCNGG 3 cut(s) 223, 304, 715
TaaI ACNGT 1 cut(s) 558
TaqI TCGA 1 cut(s) 828
TasI AATT 4 cut(s) 288, 430, 498, 625
TatI WGTACW 1 cut(s) 235
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 1 cut(s) 690
Tru1I TTAA 2 cut(s) 21, 859
Tru9I TTAA 2 cut(s) 21, 859
TseI GCWGC 5 cut(s) 14, 143, 146, 653, 800
TspDTI ATGAA 4 cut(s) 299, 724, 752, 810
TspGWI ACGGA 2 cut(s) 115, 564
Van91I CCANNNNNTGG 1 cut(s) 722
VneI GTGCAC 1 cut(s) 704
VpaK11BI GGWCC 1 cut(s) 517
VspI ATTAAT 1 cut(s) 21
XapI RAATTY 1 cut(s) 288
XceI RCATGY 2 cut(s) 731, 784
Zsp2I ATGCAT 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.