Rorug02G0472400

Carboxymethylenebutenolidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007174
Physical Location & Seq
Forward (+)
60362578 .. 60364794
2217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0472400.1

Sequence Viewer

Length: 1281 bp
ATGGAGAGCCAGAATAAGATTCCGAGTCCTGACTTGTCATCATCCACAGCAGTTGAAGAGTTTCTGGAGCAAAGAGGAGTGGCATTCAAATGCTGGCCTAGGCTTGTTGCATGGGAGTCAAGGCTCCTATGGAGTTTATCAGCTTCATCAATCATTATCTCTATAGCCAATTACATGTTCAGCTTTGTCACTCTCGCGTTTTGTGGCCATTTGAGTCCATTGGAGCTTGCTGGAGCCTCCATTGCTTGTGTGGGAATTCAAGGCCTCGCTTATGGCATCATGTTGGGGATGGCAAGTTCTGTGCAAACAGTATGTGGACAAGCATATGGAGCCAAACACTTGGGAGCAATGGGGATCATAATGCCAAAGAGCATTCATTTTACACTTGGGAGCAGCAATCCTCCTTACACCTCTGTATTGGTGCAAAGGTTCCTTCAAGCACAGAACATAGTGAACCCGTTGGCATTCATGTCTGTCGCAGTTTTCCTTCTTCACACTCTGCTCACTTGGTTGGTAGTTGATCTTGTCAATTTTGGGCTATTGGGAGCTGCTCTTACTCTCAGCTTCTCTTGGTGGTTGCTTGTAATTGCATCTGGGGTTTACATTGTTCTGAGCCCCAAGTGCAAGGAAACTTGGACTGGCTTCTCTAACAAAGCCTTCAGGGGAATTTGGCCTTATTTTAAGTTCACTGTAGCCTCTGCTTTCATGCTGTGCATGAATTACTTGAACTGGGACATGACATTTATGTTAGGCCTAAGTGCAGCTGCCAGTGTTCGAGTCGGTAATGAACTCGGGGCCGGTCATCCAAAGGTGGCCAGATTCTCAGTTTTTGTAGTGAACGTGACCAGCGTCCTGATTAGCGCAATCTTCAGTGTGATTGTATTGATCCTCCGAGTTCAGTTAAGCAAGCTATTTGCAAGTGATTCCGAGGTTATCAAGGCAGTGTCTGATTTAACTCCACTGCTCGCCATCTCTATTTTCTTGAATGGCATTCAACCTATACTCTCAGGTGTGGCAATTGGAAGTGGATGGCAAGGTGTTGTGGCTTATGTTAACTTGTTTTGTTACTATTTCATTGGTCTTCCAATTGGGTGTGTTCTTGGCTTCAAACTTAAAATAGGAGTAGCAGGTATTTGGTGGGGGATGATTATTGGAGTTTTCTTACAGACGGCAGCTCTAGTTGTCCTCACTGCCAGAACAAATTGGAATGCTCAGGTTGAAAAAGCAGCTGAGCGGTTGAAGAAATCTGCAAGTGAAGAAAACTTAAGCTTAACACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00361 ko00364 ko00623 ko01100 ko01110 ko01120 ko01130 map00361 map00364 map00623 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
DLH

Protein Analysis

426

Amino Acids

46.14

Weight (kDa)

9.06

Isoelectric Point (pI)

22.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 51 - 133 4.1e-11 MatE
MatE PF01554 233 - 364 7.7e-24 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1118
AccBSI CCGCTC 1 cut(s) 1234
AccII CGCG 1 cut(s) 197
AciI CCGC 1 cut(s) 1234
AclWI GGATC 2 cut(s) 362, 880
AcoI YGGCCR 2 cut(s) 205, 813
AcsI RAATTY 2 cut(s) 255, 666
AcuI CTGAAG 2 cut(s) 643, 853
AflII CTTAAG 1 cut(s) 1264
AflIII ACRYGT 1 cut(s) 174
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AloI GAACNNNNNNTCC 2 cut(s) 280, 312
AlwI GGATC 2 cut(s) 362, 880
AlwNI CAGNNNCTG 1 cut(s) 947
Ama87I CYCGRG 1 cut(s) 791
AoxI GGCC 7 cut(s) 95, 205, 262, 671, 751, 795, 813
ApeKI GCWGC 6 cut(s) 393, 548, 761, 764, 1172, 1226
ApoI RAATTY 2 cut(s) 255, 666
Asp700I GAANNNNTTC 1 cut(s) 60
AspA2I CCTAGG 1 cut(s) 98
AspLEI GCGC 1 cut(s) 863
AspS9I GGNCC 1 cut(s) 795
AvaI CYCGRG 1 cut(s) 791
AvrII CCTAGG 1 cut(s) 98
BalI TGGCCA 2 cut(s) 207, 815
BanII GRGCYC 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 1073
BbvI GCAGC 6 cut(s) 405, 535, 751, 773, 1184, 1238
BccI CCATC 3 cut(s) 283, 977, 1023
BceAI ACGGC 1 cut(s) 1185
BfaI CTAG 2 cut(s) 99, 1178
BfmI CTRYAG 3 cut(s) 162, 690, 1277
BfrI CTTAAG 1 cut(s) 1264
BfuAI ACCTGC 1 cut(s) 1118
BisI GCNGC 6 cut(s) 394, 549, 762, 765, 1173, 1227
BlnI CCTAGG 1 cut(s) 98
BlpI GCTNAGC 1 cut(s) 1230
BlsI GCNGC 6 cut(s) 395, 550, 763, 766, 1174, 1228
BmeT110I CYCGRG 1 cut(s) 791
BmgT120I GGNCC 1 cut(s) 795
BmiI GGNNCC 5 cut(s) 125, 235, 331, 431, 796
BmrI ACTGGG 1 cut(s) 739
BmsI GCATC 2 cut(s) 285, 599
BmuI ACTGGG 1 cut(s) 739
BoxI GACNNNNGTC 1 cut(s) 848
BpiI GAAGAC 1 cut(s) 1073
BpmI CTGGAG 2 cut(s) 86, 252
Bpu10I CCTNAGC 1 cut(s) 1212
Bpu1102I GCTNAGC 1 cut(s) 1230
BsaJI CCNNGG 2 cut(s) 98, 927
Bse118I RCCGGY 1 cut(s) 797
Bse1I ACTGG 3 cut(s) 643, 734, 768
Bse3DI GCAATG 2 cut(s) 240, 354
BseDI CCNNGG 2 cut(s) 98, 927
BseGI GGATG 5 cut(s) 41, 294, 802, 1034, 1149
BseMI GCAATG 2 cut(s) 240, 354
BseMII CTCAG 6 cut(s) 574, 602, 837, 1020, 1221, 1226
BseNI ACTGG 3 cut(s) 643, 734, 768
BseRI GAGGAG 1 cut(s) 90
BseXI GCAGC 6 cut(s) 405, 535, 751, 773, 1184, 1238
BsgI GTGCAG 1 cut(s) 780
Bsh1236I CGCG 1 cut(s) 197
BshFI GGCC 7 cut(s) 97, 207, 264, 673, 753, 797, 815
BsiHKCI CYCGRG 1 cut(s) 791
BsiSI CCGG 1 cut(s) 798
BslFI GGGAC 1 cut(s) 746
BsmFI GGGAC 1 cut(s) 746
BsmI GAATGC 5 cut(s) 83, 372, 464, 990, 1213
BsnI GGCC 7 cut(s) 97, 207, 264, 673, 753, 797, 815
BsoBI CYCGRG 1 cut(s) 791
Bsp1286I GDGCHC 1 cut(s) 617
Bsp143I GATC 3 cut(s) 354, 520, 885
Bsp1720I GCTNAGC 1 cut(s) 1230
BspACI CCGC 1 cut(s) 1234
BspANI GGCC 7 cut(s) 97, 207, 264, 673, 753, 797, 815
BspCNI CTCAG 6 cut(s) 573, 603, 836, 1019, 1222, 1225
BspFNI CGCG 1 cut(s) 197
BspLI GGNNCC 5 cut(s) 125, 235, 331, 431, 796
BspMI ACCTGC 1 cut(s) 1118
BspPI GGATC 2 cut(s) 362, 880
BspTI CTTAAG 1 cut(s) 1264
BsrBI CCGCTC 1 cut(s) 1234
BsrDI GCAATG 2 cut(s) 240, 354
BsrFI RCCGGY 1 cut(s) 797
BsrI ACTGG 3 cut(s) 643, 734, 768
BssAI RCCGGY 1 cut(s) 797
BssECI CCNNGG 2 cut(s) 98, 927
BssMI GATC 3 cut(s) 354, 520, 885
BssT1I CCWWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 310, 691
Bst6I CTCTTC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 1264
BstC8I GCNNGC 4 cut(s) 95, 228, 908, 966
BstDEI CTNAG 7 cut(s) 560, 611, 755, 823, 1006, 1212, 1230
BstF5I GGATG 5 cut(s) 41, 294, 802, 1034, 1149
BstFNI CGCG 1 cut(s) 197
BstHHI GCGC 1 cut(s) 863
BstKTI GATC 3 cut(s) 357, 523, 888
BstMBI GATC 3 cut(s) 354, 520, 885
BstMWI GCNNNNNNNGC 3 cut(s) 242, 329, 621
BstNSI RCATGY 1 cut(s) 178
BstPAI GACNNNNGTC 1 cut(s) 848
BstSFI CTRYAG 3 cut(s) 162, 690, 1277
BstUI CGCG 1 cut(s) 197
BstV1I GCAGC 6 cut(s) 405, 535, 751, 773, 1184, 1238
BstV2I GAAGAC 1 cut(s) 1073
BstXI CCANNNNNNTGG 1 cut(s) 340
BsuRI GGCC 7 cut(s) 97, 207, 264, 673, 753, 797, 815
BtsCI GGATG 5 cut(s) 41, 294, 802, 1034, 1149
BtsI GCAGTG 3 cut(s) 948, 959, 1188
BtsIMutI CAGTG 6 cut(s) 687, 775, 877, 948, 959, 1188
BveI ACCTGC 1 cut(s) 1118
Cac8I GCNNGC 4 cut(s) 95, 228, 908, 966
CaiI CAGNNNCTG 1 cut(s) 947
CfoI GCGC 1 cut(s) 863
Cfr10I RCCGGY 1 cut(s) 797
Cfr13I GGNCC 1 cut(s) 795
CseI GACGC 1 cut(s) 838
CspCI CAANNNNNGTGG 2 cut(s) 34, 69
CviAII CATG 7 cut(s) 111, 175, 280, 469, 706, 715, 736
DdeI CTNAG 7 cut(s) 560, 611, 755, 823, 1006, 1212, 1230
DpnI GATC 3 cut(s) 356, 522, 887
DpnII GATC 3 cut(s) 354, 520, 885
EaeI YGGCCR 2 cut(s) 205, 813
Eam1104I CTCTTC 1 cut(s) 51
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 98
Eco147I AGGCCT 2 cut(s) 264, 753
Eco24I GRGCYC 1 cut(s) 617
Eco57I CTGAAG 2 cut(s) 643, 853
Eco88I CYCGRG 1 cut(s) 791
EcoRI GAATTC 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 98
FaeI CATG 7 cut(s) 114, 178, 283, 472, 709, 718, 739
FaqI GGGAC 1 cut(s) 746
FatI CATG 7 cut(s) 110, 174, 279, 468, 705, 714, 735
FauNDI CATATG 1 cut(s) 325
Fnu4HI GCNGC 6 cut(s) 394, 549, 762, 765, 1173, 1227
FokI GGATG 5 cut(s) 28, 301, 789, 1041, 1156
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 6 cut(s) 394, 549, 762, 765, 1173, 1227
FspBI CTAG 2 cut(s) 99, 1178
GlaI GCGC 1 cut(s) 862
GluI GCNGC 6 cut(s) 394, 549, 762, 765, 1173, 1227
GsuI CTGGAG 2 cut(s) 86, 252
HaeIII GGCC 7 cut(s) 97, 207, 264, 673, 753, 797, 815
HapII CCGG 1 cut(s) 798
HgaI GACGC 1 cut(s) 838
HhaI GCGC 1 cut(s) 863
Hin1II CATG 7 cut(s) 114, 178, 283, 472, 709, 718, 739
Hin6I GCGC 1 cut(s) 861
HinP1I GCGC 1 cut(s) 861
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 1 cut(s) 1267
HinfI GANTC 7 cut(s) 19, 25, 116, 214, 777, 819, 923
HpaI GTTAAC 1 cut(s) 1054
HpaII CCGG 1 cut(s) 798
Hpy166II GTNNAC 6 cut(s) 317, 454, 601, 687, 838, 1054
Hpy188I TCNGA 5 cut(s) 24, 612, 893, 928, 949
Hpy188III TCNNGA 4 cut(s) 29, 65, 853, 982
Hpy8I GTNNAC 6 cut(s) 317, 454, 601, 687, 838, 1054
HpyAV CCTTC 3 cut(s) 443, 497, 667
HpyCH4III ACNGT 2 cut(s) 310, 691
HpyCH4IV ACGT 1 cut(s) 840
HpyCH4V TGCA 9 cut(s) 110, 304, 424, 590, 624, 714, 761, 917, 1250
HpyF10VI GCNNNNNNNGC 3 cut(s) 242, 329, 621
HpyF3I CTNAG 7 cut(s) 560, 611, 755, 823, 1006, 1212, 1230
HpySE526I ACGT 1 cut(s) 840
Hsp92II CATG 7 cut(s) 114, 178, 283, 472, 709, 718, 739
HspAI GCGC 1 cut(s) 861
KspAI GTTAAC 1 cut(s) 1054
Kzo9I GATC 3 cut(s) 354, 520, 885
LmnI GCTCC 8 cut(s) 67, 129, 223, 233, 329, 344, 390, 545
Lsp1109I GCAGC 6 cut(s) 405, 535, 751, 773, 1184, 1238
LweI GCATC 2 cut(s) 285, 599
MaeI CTAG 2 cut(s) 99, 1178
MaeII ACGT 1 cut(s) 840
MaeIII GTNAC 3 cut(s) 187, 841, 1064
MalI GATC 3 cut(s) 356, 522, 887
MbiI CCGCTC 1 cut(s) 1234
MboI GATC 3 cut(s) 354, 520, 885
MboII GAAGA 6 cut(s) 68, 482, 859, 1073, 1252, 1268
MfeI CAATTG 2 cut(s) 1017, 1086
MhlI GDGCHC 1 cut(s) 617
MlsI TGGCCA 2 cut(s) 207, 815
MluCI AATT 9 cut(s) 169, 255, 529, 585, 666, 718, 1017, 1086, 1201
MluNI TGGCCA 2 cut(s) 207, 815
MlyI GAGTC 4 cut(s) 34, 125, 223, 786
MnlI CCTC 9 cut(s) 68, 247, 275, 411, 421, 706, 899, 922, 1196
Mox20I TGGCCA 2 cut(s) 207, 815
MroXI GAANNNNTTC 1 cut(s) 60
MscI TGGCCA 2 cut(s) 207, 815
MseI TTAA 7 cut(s) 681, 902, 953, 1053, 1113, 1265, 1271
MslI CAYNNNNRTG 1 cut(s) 88
Msp20I TGGCCA 2 cut(s) 207, 815
MspA1I CMGCKG 2 cut(s) 764, 1229
MspCI CTTAAG 1 cut(s) 1264
MspI CCGG 1 cut(s) 798
MunI CAATTG 2 cut(s) 1017, 1086
Mva1269I GAATGC 5 cut(s) 83, 372, 464, 990, 1213
MvnI CGCG 1 cut(s) 197
MwoI GCNNNNNNNGC 3 cut(s) 242, 329, 621
NdeI CATATG 1 cut(s) 325
NdeII GATC 3 cut(s) 354, 520, 885
NlaIII CATG 7 cut(s) 114, 178, 283, 472, 709, 718, 739
NlaIV GGNNCC 5 cut(s) 125, 235, 331, 431, 796
NmuCI GTSAC 2 cut(s) 187, 841
NspI RCATGY 1 cut(s) 178
PceI AGGCCT 2 cut(s) 264, 753
PciI ACATGT 1 cut(s) 174
PcsI WCGNNNNNNNCGW 1 cut(s) 846
PctI GAATGC 5 cut(s) 83, 372, 464, 990, 1213
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 3 cut(s) 19, 819, 923
PkrI GCNGC 6 cut(s) 395, 550, 763, 766, 1174, 1228
PleI GAGTC 4 cut(s) 33, 124, 222, 785
PpsI GAGTC 4 cut(s) 33, 124, 222, 785
PscI ACATGT 1 cut(s) 174
PshAI GACNNNNGTC 1 cut(s) 848
PspN4I GGNNCC 5 cut(s) 125, 235, 331, 431, 796
PspPI GGNCC 1 cut(s) 795
PstNI CAGNNNCTG 1 cut(s) 947
PvuII CAGCTG 2 cut(s) 764, 1229
RseI CAYNNNNRTG 1 cut(s) 88
SaqAI TTAA 7 cut(s) 681, 902, 953, 1053, 1113, 1265, 1271
SatI GCNGC 6 cut(s) 394, 549, 762, 765, 1173, 1227
Sau3AI GATC 3 cut(s) 354, 520, 885
Sau96I GGNCC 1 cut(s) 795
SchI GAGTC 4 cut(s) 34, 125, 223, 786
SduI GDGCHC 1 cut(s) 617
SfaNI GCATC 2 cut(s) 285, 599
SfcI CTRYAG 3 cut(s) 162, 690, 1277
SmiMI CAYNNNNRTG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 1264
SmoI CTYRAG 1 cut(s) 1264
Sse9I AATT 9 cut(s) 169, 255, 529, 585, 666, 718, 1017, 1086, 1201
SseBI AGGCCT 2 cut(s) 264, 753
SsiI CCGC 1 cut(s) 1234
SspMI CTAG 2 cut(s) 99, 1178
StuI AGGCCT 2 cut(s) 264, 753
StyI CCWWGG 1 cut(s) 98
TaaI ACNGT 2 cut(s) 310, 691
TaiI ACGT 1 cut(s) 843
TaqI TCGA 1 cut(s) 775
TasI AATT 9 cut(s) 169, 255, 529, 585, 666, 718, 1017, 1086, 1201
TfiI GAWTC 3 cut(s) 19, 819, 923
Tru1I TTAA 7 cut(s) 681, 902, 953, 1053, 1113, 1265, 1271
Tru9I TTAA 7 cut(s) 681, 902, 953, 1053, 1113, 1265, 1271
TscAI CASTG 6 cut(s) 694, 775, 877, 948, 966, 1195
TseFI GTSAC 2 cut(s) 187, 841
TseI GCWGC 6 cut(s) 393, 548, 761, 764, 1172, 1226
Tsp45I GTSAC 2 cut(s) 187, 841
TspDTI ATGAA 7 cut(s) 135, 365, 457, 694, 731, 801, 1063
TspRI CASTG 6 cut(s) 694, 775, 877, 948, 966, 1195
Vha464I CTTAAG 1 cut(s) 1264
XapI RAATTY 2 cut(s) 255, 666
XceI RCATGY 1 cut(s) 178
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XmaJI CCTAGG 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 2 cut(s) 99, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.